Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3.

abstract：:Type I interferons (IFNs) are multifunctional cytokines that regulate immune responses and cellular functions but also can have detrimental effects on human health. A tight regulatory network therefore controls IFN signaling, which in turn may interfere with medical interventions. The JAK-STAT signaling pathway transm...

journal_title：Nature structural & molecular biology

authors： Arimoto KI,Löchte S,Stoner SA,Burkart C,Zhang Y,Miyauchi S,Wilmes S,Fan JB,Heinisch JJ,Li Z,Yan M,Pellegrini S,Colland F,Piehler J,Zhang DE

更新日期：2017-03-01 00:00:00

abstract：:We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show...

journal_title：Nature structural & molecular biology

authors： Kortemme T,Joachimiak LA,Bullock AN,Schuler AD,Stoddard BL,Baker D

更新日期：2004-04-01 00:00:00

abstract：:Messenger RNAs produced by splicing are translated more efficiently than those produced from similar intronless precursor mRNAs (pre-mRNAs). The exon-junction complex (EJC) probably mediates this enhancement; however, the specific link between the EJC and the translation machinery has not been identified. The EJC prot...

journal_title：Nature structural & molecular biology

authors： Diem MD,Chan CC,Younis I,Dreyfuss G

更新日期：2007-12-01 00:00:00

abstract：:DNA methylation is an epigenetic modification that is generally associated with repression of transcription initiation at CpG-island promoters. Here we argue that, on the basis of recent high-throughput genomic and proteomic screenings, DNA methylation can also have different outcomes, including activation of transcri...

journal_title：Nature structural & molecular biology

authors： Spruijt CG,Vermeulen M

更新日期：2014-11-01 00:00:00

abstract：:The Saccharomyces cerevisiae proteasome comprises a 19-subunit regulatory particle and a 28-subunit core particle. To be degraded, substrates must cross the core particle-regulatory particle interface, a site for complex conformational changes and regulatory events. This interface includes two aligned heteromeric ring...

journal_title：Nature structural & molecular biology

authors： Tian G,Park S,Lee MJ,Huck B,McAllister F,Hill CP,Gygi SP,Finley D

更新日期：2011-10-30 00:00:00

abstract：:In eukaryotic cells, many introns are constitutively, rather than alternatively, spliced and therefore do not contribute to isoform diversification. It has remained unclear what functional roles such constitutive splicing provides. To explore this issue, we asked how splicing affects the efficiency with which individu...

journal_title：Nature structural & molecular biology

authors： Ding F,Elowitz MB

更新日期：2019-06-01 00:00:00

abstract：:The tandem zinc finger (TZF) domain of the protein TIS11d binds to the class II AU-rich element (ARE) in the 3' untranslated region (3' UTR) of target mRNAs and promotes their deadenylation and degradation. The NMR structure of the TIS11d TZF domain bound to the RNA sequence 5'-UUAUUUAUU-3' comprises a pair of novel C...

journal_title：Nature structural & molecular biology

authors： Hudson BP,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2004-03-01 00:00:00

abstract：:Ubiquitination is a post-translational modification (PTM) that is essential for balancing numerous physiological processes. To enable delineation of protein ubiquitination at a site-specific level, we generated an antibody, denoted UbiSite, recognizing the C-terminal 13 amino acids of ubiquitin, which remain attached ...

journal_title：Nature structural & molecular biology

authors： Akimov V,Barrio-Hernandez I,Hansen SVF,Hallenborg P,Pedersen AK,Bekker-Jensen DB,Puglia M,Christensen SDK,Vanselow JT,Nielsen MM,Kratchmarova I,Kelstrup CD,Olsen JV,Blagoev B

更新日期：2018-07-01 00:00:00

abstract：:Ribosomes catalyze the formation of peptide bonds between aminoacyl esters of transfer RNAs within a catalytic center composed of ribosomal RNA only. Here we show that the reaction of P-site formylmethionine (fMet)-tRNA(fMet) with a modified A-site tRNA substrate, Phelac-tRNA(Phe), in which the nucleophilic amino grou...

journal_title：Nature structural & molecular biology

authors： Bieling P,Beringer M,Adio S,Rodnina MV

更新日期：2006-05-01 00:00:00

abstract：:Noncoding RNAs, mobile elements, and alternative splicing are all critical for the regulation of gene expression. Here we show that a conserved noncoding RNA acquires a new function due to the insertion of a mobile element. We identified a noncoding RNA, termed 5S-OT, which is transcribed from 5S rDNA loci in eukaryot...

journal_title：Nature structural & molecular biology

authors： Hu S,Wang X,Shan G

更新日期：2016-11-01 00:00:00

abstract：:Domain 5 (D5) is the central core of group II intron ribozymes. Many base and backbone substituents of this highly conserved hairpin participate in catalysis and are crucial for binding to other intron domains. We report the solution structures of the 34-nucleotide D5 hairpin from the group II intron ai5 gamma in the ...

journal_title：Nature structural & molecular biology

authors： Sigel RK,Sashital DG,Abramovitz DL,Palmer AG,Butcher SE,Pyle AM

更新日期：2004-02-01 00:00:00

abstract：:An increasing body of evidence indicates that transcription and splicing are coupled, and it is accepted that chromatin organization regulates transcription. Little is known about the cross-talk between chromatin structure and exon-intron architecture. By analysis of genome-wide nucleosome-positioning data sets from h...

journal_title：Nature structural & molecular biology

authors： Schwartz S,Meshorer E,Ast G

更新日期：2009-09-01 00:00:00

abstract：:Complexes containing a pair of structural maintenance of chromosomes (SMC) family proteins are fundamental for the three-dimensional (3D) organization of genomes in all domains of life. The eukaryotic SMC complexes cohesin and condensin are thought to fold interphase and mitotic chromosomes, respectively, into large l...

journal_title：Nature structural & molecular biology

authors： Lee BG,Merkel F,Allegretti M,Hassler M,Cawood C,Lecomte L,O'Reilly FJ,Sinn LR,Gutierrez-Escribano P,Kschonsak M,Bravo S,Nakane T,Rappsilber J,Aragon L,Beck M,Löwe J,Haering CH

更新日期：2020-08-01 00:00:00

abstract：:Although the two B-family human DNA polymerases, pol δ and pol ε, are responsible for the bulk of nuclear genome replication, at least 14 additional polymerases have roles in nuclear DNA repair and replication. In this issue, newly reported crystal structures of two specialized A-family polymerases, pol δ and pol ε, e...

journal_title：Nature structural & molecular biology

authors： Beard WA,Wilson SH

更新日期：2015-04-01 00:00:00

abstract：:Telomeres may regulate human disease by at least two independent mechanisms. First, replicative senescence occurs once short telomeres generate DNA-damage signals that produce a barrier to tumor progression. Second, telomere position effects (TPE) could change gene expression at intermediate telomere lengths in cultur...

journal_title：Nature structural & molecular biology

authors： Stadler G,Rahimov F,King OD,Chen JC,Robin JD,Wagner KR,Shay JW,Emerson CP Jr,Wright WE

更新日期：2013-06-01 00:00:00

abstract：:Core Qβ replicase comprises the Qβ virus-encoded RNA-dependent RNA polymerase (β-subunit) and the host Escherichia coli translational elongation factors EF-Tu and EF-Ts. The functions of the host proteins in the viral replicase are not clear. Structural analyses of RNA polymerization by core Qβ replicase reveal that a...

journal_title：Nature structural & molecular biology

authors： Takeshita D,Tomita K

更新日期：2012-01-15 00:00:00

abstract：:STING functions as both an adaptor protein signaling cytoplasmic double-stranded DNA and a direct immunosensor of cyclic diguanylate monophosphate (c-di-GMP). The crystal structures of the C-terminal domain of human STING (STING(CTD)) and its complex with c-di-GMP reveal how STING recognizes c-di-GMP. In response to c...

journal_title：Nature structural & molecular biology

authors： Shang G,Zhu D,Li N,Zhang J,Zhu C,Lu D,Liu C,Yu Q,Zhao Y,Xu S,Gu L

更新日期：2012-06-24 00:00:00

abstract：:The Mre11-Rad50-NBS1 (MRN) complex has many roles in response to DNA double-strand breaks, but its functions in repair by nonhomologous end joining (NHEJ) pathways are poorly understood. We have investigated requirements for MRN in class switch recombination (CSR), a programmed DNA rearrangement in B lymphocytes that ...

journal_title：Nature structural & molecular biology

authors： Dinkelmann M,Spehalski E,Stoneham T,Buis J,Wu Y,Sekiguchi JM,Ferguson DO

更新日期：2009-08-01 00:00:00

abstract：:During homologous recombination, Rad51 forms a nucleoprotein filament with single-stranded DNA (ssDNA) that undergoes strand exchange with homologous double-stranded DNA (dsDNA). Here, we use real-time analysis to show that strand exchange by fission yeast Rad51 proceeds via two distinct three-strand intermediates, C1...

journal_title：Nature structural & molecular biology

authors： Ito K,Murayama Y,Takahashi M,Iwasaki H

更新日期：2018-01-01 00:00:00

abstract：:MCL-1 is an antiapoptotic BCL-2 family protein that has emerged as a major pathogenic factor in human cancer. Like BCL-2, MCL-1 bears a surface groove whose function is to sequester the BH3 killer domains of proapoptotic BCL-2 family members, a mechanism harnessed by cancer cells to establish formidable apoptotic bloc...

journal_title：Nature structural & molecular biology

authors： Lee S,Wales TE,Escudero S,Cohen DT,Luccarelli J,Gallagher CG,Cohen NA,Huhn AJ,Bird GH,Engen JR,Walensky LD

更新日期：2016-06-01 00:00:00

abstract：:No-go decay (NGD) targets mRNAs with stalls in translation elongation for endonucleolytic cleavage in a process involving the Dom34 and Hbs1 proteins. The crystal structure of a Schizosaccharomyces pombe Dom34-Hbs1 complex reveals an overall shape similar to that of eRF1-eRF3-GTP and EF-Tu-tRNA-GDPNP. Similarly to eRF...

journal_title：Nature structural & molecular biology

authors： Chen L,Muhlrad D,Hauryliuk V,Cheng Z,Lim MK,Shyp V,Parker R,Song H

更新日期：2010-10-01 00:00:00

abstract：:Telomere capping conceals chromosome ends from exonucleases and checkpoints, but the full range of capping mechanisms is not well defined. Telomeres have the potential to form G-quadruplex (G4) DNA, although evidence for telomere G4 DNA function in vivo is limited. In budding yeast, capping requires the Cdc13 protein ...

journal_title：Nature structural & molecular biology

authors： Smith JS,Chen Q,Yatsunyk LA,Nicoludis JM,Garcia MS,Kranaster R,Balasubramanian S,Monchaud D,Teulade-Fichou MP,Abramowitz L,Schultz DC,Johnson FB

更新日期：2011-04-01 00:00:00

abstract：:We developed an experimental system for studying genome instability caused by fragile X (CGG)n repeats in mammalian cells. Our method uses a selectable cassette carrying the HyTK gene under the control of the FMR1 promoter with (CGG)n repeats in its 5' UTR, which is integrated into the unique RL5 site in murine erythr...

journal_title：Nature structural & molecular biology

authors： Kononenko AV,Ebersole T,Vasquez KM,Mirkin SM

更新日期：2018-08-01 00:00:00

abstract：:The potassium channel selectivity filter both discriminates between K(+) and sodium ions and contributes to gating of ion flow. Static structures of conducting (open) and nonconducting (inactivated) conformations of this filter are known; however, the sequence of protein rearrangements that connect these two states is...

journal_title：Nature structural & molecular biology

authors： Wang DT,Hill AP,Mann SA,Tan PS,Vandenberg JI

更新日期：2011-01-01 00:00:00

abstract：:Myotonic dystrophy is an RNA gain-of-function disease caused by expanded CUG or CCUG repeats, which sequester the RNA binding protein MBNL1. Here we describe a newly discovered function for MBNL1 as a regulator of pre-miR-1 biogenesis and find that miR-1 processing is altered in heart samples from people with myotonic...

journal_title：Nature structural & molecular biology

authors： Rau F,Freyermuth F,Fugier C,Villemin JP,Fischer MC,Jost B,Dembele D,Gourdon G,Nicole A,Duboc D,Wahbi K,Day JW,Fujimura H,Takahashi MP,Auboeuf D,Dreumont N,Furling D,Charlet-Berguerand N

更新日期：2011-06-19 00:00:00

abstract：:Core Factor (CF) is a conserved RNA polymerase (Pol) I general transcription factor comprising Rrn6, Rrn11 and the TFIIB-related subunit Rrn7. CF binds TATA-binding protein (TBP), Pol I and the regulatory factors Rrn3 and upstream activation factor. We used chemical cross-linking-MS to determine the molecular architec...

journal_title：Nature structural & molecular biology

authors： Knutson BA,Luo J,Ranish J,Hahn S

更新日期：2014-09-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Putnam A,Cassani M,Smith J,Seydoux G

更新日期：2020-02-01 00:00:00

abstract：:Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatch...

journal_title：Nature structural & molecular biology

authors： Liu X,Zhang Q,Murata K,Baker ML,Sullivan MB,Fu C,Dougherty MT,Schmid MF,Osburne MS,Chisholm SW,Chiu W

更新日期：2010-07-01 00:00:00

abstract：:Tripeptidyl peptidase II (TPP II) is the largest known eukaryotic protease (6 MDa). It is believed to act downstream of the 26S proteasome, cleaving tripeptides from the N termini of longer peptides, and it is implicated in numerous cellular processes. Here we report the structure of Drosophila TPP II determined by a ...

journal_title：Nature structural & molecular biology

authors： Chuang CK,Rockel B,Seyit G,Walian PJ,Schönegge AM,Peters J,Zwart PH,Baumeister W,Jap BK

更新日期：2010-08-01 00:00:00

abstract：:The ≥ 10³⁰ bacteriophages on Earth relentlessly drive adaptive coevolution, forcing the generation of protective mechanisms in their bacterial hosts. One such bacterial phage-resistance system, ToxIN, consists of a protein toxin (ToxN) that is inhibited in vivo by a specific RNA antitoxin (ToxI); however, the mechanis...

journal_title：Nature structural & molecular biology

authors： Blower TR,Pei XY,Short FL,Fineran PC,Humphreys DP,Luisi BF,Salmond GP

更新日期：2011-02-01 00:00:00