当前位置： SCI文献检索 > NATURE STRUCTURAL & MOLECULAR BIOLOGY期刊下所有文献 > Conformational restriction blocks glutamate receptor desensitization.

Conformational restriction blocks glutamate receptor desensitization.

Abstract：

:Desensitization is a universal feature of ligand-gated ion channels. Using the crystal structure of the GluR2 L483Y mutant channel as a guide, we attempted to build non-desensitizing kainate-subtype glutamate receptors. Success was achieved for GluR5, GluR6 and GluR7 with intermolecular disulfide cross-links but not by engineering the dimer interface. Crystallographic analysis of the GluR6 Y490C L752C dimer revealed relaxation from the active conformation, which functional studies reveal is not sufficient to trigger desensitization. The equivalent non-desensitizing cross-linked GluR2 mutant retained weak sensitivity to a positive allosteric modulator, which had no effect on GluR2 L483Y. These results establish that the active conformation of AMPA and kainate receptors is conserved and further show that their desensitization requires dimer rearrangements, that subtle structural differences account for their diverse functional properties and that the ligand-binding core dimer is a powerful regulator of ion-channel activity.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Weston MC,Schuck P,Ghosal A,Rosenmund C,Mayer ML

doi

10.1038/nsmb1178

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

1120-7

issue

12

eissn

1545-9993

issn

1545-9985

pii

nsmb1178

journal_volume

13

pub_type

杂志文章

相关文献

NATURE STRUCTURAL & MOLECULAR BIOLOGY文献大全
  • Structure of a kinesin-tubulin complex and implications for kinesin motility.

    abstract：:The typical function of kinesins is to transport cargo along microtubules. Binding of ATP to microtubule-attached motile kinesins leads to cargo displacement. To better understand the nature of the conformational changes that lead to the power stroke that moves a kinesin's load along a microtubule, we determined the X...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2624

    authors： Gigant B,Wang W,Dreier B,Jiang Q,Pecqueur L,Plückthun A,Wang C,Knossow M

    更新日期：2013-08-01 00:00:00

  • RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation.

    abstract：:The RNA polymerase II (RNAPII) C-terminal domain (CTD) heptapeptide repeats (1-YSPTSPS-7) undergo dynamic phosphorylation and dephosphorylation during the transcription cycle to recruit factors that regulate transcription, RNA processing and chromatin modification. We show here that RPRD1A and RPRD1B form homodimers a...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2853

    authors： Ni Z,Xu C,Guo X,Hunter GO,Kuznetsova OV,Tempel W,Marcon E,Zhong G,Guo H,Kuo WW,Li J,Young P,Olsen JB,Wan C,Loppnau P,El Bakkouri M,Senisterra GA,He H,Huang H,Sidhu SS,Emili A,Murphy S,Mosley AL,Arrowsmith CH

    更新日期：2014-08-01 00:00:00

  • Stochastic expression dynamics of a transcription factor revealed by single-molecule noise analysis.

    abstract：:Gene expression is inherently stochastic; precise gene regulation by transcription factors is important for cell-fate determination. Many transcription factors regulate their own expression, suggesting that autoregulation counters intrinsic stochasticity in gene expression. Using a new strategy, cotranslational activa...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2336

    authors： Hensel Z,Feng H,Han B,Hatem C,Wang J,Xiao J

    更新日期：2012-08-01 00:00:00

  • Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex.

    abstract：:The iota toxin produced by Clostridium perfringens type E is a binary toxin comprising two independent polypeptides: Ia, an ADP-ribosyltransferase, and Ib, which is involved in cell binding and translocation of Ia across the cell membrane. Here we report cryo-EM structures of the translocation channel Ib-pore and its ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0388-6

    authors： Yamada T,Yoshida T,Kawamoto A,Mitsuoka K,Iwasaki K,Tsuge H

    更新日期：2020-03-01 00:00:00

  • Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA.

    abstract：:In eukaryotes, the Cdt1-bound replicative helicase core MCM2-7 is loaded onto DNA by the ORC-Cdc6 ATPase to form a prereplicative complex (pre-RC) with an MCM2-7 double hexamer encircling DNA. Using purified components in the presence of ATP-γS, we have captured in vitro an intermediate in pre-RC assembly that contain...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2629

    authors： Sun J,Evrin C,Samel SA,Fernández-Cid A,Riera A,Kawakami H,Stillman B,Speck C,Li H

    更新日期：2013-08-01 00:00:00

  • A protein-based EM label for RNA identifies the location of exons in spliceosomes.

    abstract：:To locate key RNA features in the structure of the spliceosome by EM, we fused a sequence-specific RNA binding protein to a protein with a distinct donut-shaped structure. We used this fusion to label spliceosomes assembled on a pre-mRNA that contained the target sequence in the exons. The label is clearly visible in ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1378

    authors： Alcid EA,Jurica MS

    更新日期：2008-02-01 00:00:00

  • Dynamic enzyme docking to the ribosome coordinates N-terminal processing with polypeptide folding.

    abstract：:Newly synthesized polypeptides undergo various cotranslational maturation steps, including N-terminal enzymatic processing, chaperone-assisted folding and membrane targeting, but the spatial and temporal coordination of these steps is unclear. We show that Escherichia coli methionine aminopeptidase (MAP) associates wi...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2615

    authors： Sandikci A,Gloge F,Martinez M,Mayer MP,Wade R,Bukau B,Kramer G

    更新日期：2013-07-01 00:00:00

  • Publisher Correction: Tauopathies: Protein shapes at the core of chronic traumatic encephalopathy.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature structural & molecular biology

    pub_type： 已发布勘误

    doi：10.1038/s41594-019-0251-9

    authors： Fichou Y,Han S

    更新日期：2019-06-01 00:00:00

  • Structural basis for protein-RNA recognition in telomerase.

    abstract：:Telomerase is a large ribonucleoprotein complex minimally composed of a catalytic telomerase reverse transcriptase (TERT) and an RNA component (TR) that provides the template for telomeric DNA synthesis. However, it remains unclear how TERT and TR assemble into a functional telomerase. Here we report the crystal struc...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2819

    authors： Huang J,Brown AF,Wu J,Xue J,Bley CJ,Rand DP,Wu L,Zhang R,Chen JJ,Lei M

    更新日期：2014-06-01 00:00:00

  • Structural basis for SENP2 protease interactions with SUMO precursors and conjugated substrates.

    abstract：:SUMO processing and deconjugation are essential proteolytic activities for nuclear metabolism and cell-cycle progression in yeast and higher eukaryotes. To elucidate the mechanisms used during substrate lysine deconjugation, SUMO isoform processing and SUMO isoform interactions, X-ray structures were determined for a ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1168

    authors： Reverter D,Lima CD

    更新日期：2006-12-01 00:00:00

  • Misregulation of miR-1 processing is associated with heart defects in myotonic dystrophy.

    abstract：:Myotonic dystrophy is an RNA gain-of-function disease caused by expanded CUG or CCUG repeats, which sequester the RNA binding protein MBNL1. Here we describe a newly discovered function for MBNL1 as a regulator of pre-miR-1 biogenesis and find that miR-1 processing is altered in heart samples from people with myotonic...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2067

    authors： Rau F,Freyermuth F,Fugier C,Villemin JP,Fischer MC,Jost B,Dembele D,Gourdon G,Nicole A,Duboc D,Wahbi K,Day JW,Fujimura H,Takahashi MP,Auboeuf D,Dreumont N,Furling D,Charlet-Berguerand N

    更新日期：2011-06-19 00:00:00

  • MRE11-RAD50-NBS1 and ATM function as co-mediators of TRF1 in telomere length control.

    abstract：:Human telomeres are associated with ATM and the protein complex consisting of MRE11, RAD50 and NBS1 (MRN), which are central to maintaining genomic stability. Here we show that when targeted to telomeres, wild-type RAD50 downregulates telomeric association of TRF1, a negative regulator of telomere maintenance. TRF1 bi...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1286

    authors： Wu Y,Xiao S,Zhu XD

    更新日期：2007-09-01 00:00:00

  • Structural basis of the proinflammatory signaling complex mediated by TSLP.

    abstract：:Thymic stromal lymphopoietin (TSLP), a cytokine produced by epithelial cells at barrier surfaces, is pivotal for the development of widespread chronic inflammatory disorders such as asthma and atopic dermatitis. The structure of the mouse TSLP-mediated signaling complex reveals how TSLP establishes extensive interface...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2794

    authors： Verstraete K,van Schie L,Vyncke L,Bloch Y,Tavernier J,Pauwels E,Peelman F,Savvides SN

    更新日期：2014-04-01 00:00:00

  • Multiple functions of MRN in end-joining pathways during isotype class switching.

    abstract：:The Mre11-Rad50-NBS1 (MRN) complex has many roles in response to DNA double-strand breaks, but its functions in repair by nonhomologous end joining (NHEJ) pathways are poorly understood. We have investigated requirements for MRN in class switch recombination (CSR), a programmed DNA rearrangement in B lymphocytes that ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1639

    authors： Dinkelmann M,Spehalski E,Stoneham T,Buis J,Wu Y,Sekiguchi JM,Ferguson DO

    更新日期：2009-08-01 00:00:00

  • Simultaneous visualization of the extracellular and cytoplasmic domains of the epidermal growth factor receptor.

    abstract：:To our knowledge, no structural study to date has characterized, in an intact receptor, the coupling of conformational change in extracellular domains through a single-pass transmembrane domain to conformational change in cytoplasmic domains. Here we examine such coupling, and its unexpected complexity, using nearly f...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2092

    authors： Mi LZ,Lu C,Li Z,Nishida N,Walz T,Springer TA

    更新日期：2011-08-07 00:00:00

  • Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation.

    abstract：:The AAA+ ATPase Vps4 disassembles ESCRT-III and is essential for HIV-1 budding and other pathways. Vps4 is a paradigmatic member of a class of hexameric AAA+ ATPases that disassemble protein complexes without degradation. To distinguish between local displacement versus global unfolding mechanisms for complex disassem...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3015

    authors： Yang B,Stjepanovic G,Shen Q,Martin A,Hurley JH

    更新日期：2015-06-01 00:00:00

  • Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.

    abstract：:Point mutations in cysteine string protein-α (CSPα) cause dominantly inherited adult-onset neuronal ceroid lipofuscinosis (ANCL), a rapidly progressing and lethal neurodegenerative disease with no treatment. ANCL mutations are proposed to trigger CSPα aggregation/oligomerization, but the mechanism of oligomer formatio...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0375-y

    authors： Naseri NN,Ergel B,Kharel P,Na Y,Huang Q,Huang R,Dolzhanskaya N,Burré J,Velinov MT,Sharma M

    更新日期：2020-02-01 00:00:00

  • Crystal structure of the tubulin tyrosine carboxypeptidase complex VASH1-SVBP.

    abstract：:The cyclic enzymatic removal and ligation of the C-terminal tyrosine of α-tubulin generates heterogeneous microtubules and affects their functions. Here we describe the crystal and solution structure of the tubulin carboxypeptidase complex between vasohibin (VASH1) and small vasohibin-binding protein (SVBP), which fol...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-019-0254-6

    authors： Adamopoulos A,Landskron L,Heidebrecht T,Tsakou F,Bleijerveld OB,Altelaar M,Nieuwenhuis J,Celie PHN,Brummelkamp TR,Perrakis A

    更新日期：2019-07-01 00:00:00

  • Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome.

    abstract：:SIX1 interacts with EYA to form a bipartite transcription factor essential for mammalian development. Loss of function of this complex causes branchio-oto-renal (BOR) syndrome, whereas re-expression of SIX1 or EYA promotes metastasis. Here we describe the 2.0-Å structure of SIX1 bound to EYA2, which suggests a new DNA...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2505

    authors： Patrick AN,Cabrera JH,Smith AL,Chen XS,Ford HL,Zhao R

    更新日期：2013-04-01 00:00:00

  • Molecular basis of histone H3K36me3 recognition by the PWWP domain of Brpf1.

    abstract：:Trimethylation of Lys36 in histone H3 (H3K36me3) coordinates events associated with the elongation phase of transcription and is also emerging as an important epigenetic regulator of cell growth and differentiation. We have identified the PWWP domain of bromo and plant homeodomain (PHD) finger-containing protein 1 (BR...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1797

    authors： Vezzoli A,Bonadies N,Allen MD,Freund SM,Santiveri CM,Kvinlaug BT,Huntly BJ,Göttgens B,Bycroft M

    更新日期：2010-05-01 00:00:00

  • Proton-powered subunit rotation in single membrane-bound F0F1-ATP synthase.

    abstract：:Synthesis of ATP from ADP and phosphate, catalyzed by F(0)F(1)-ATP synthases, is the most abundant physiological reaction in almost any cell. F(0)F(1)-ATP synthases are membrane-bound enzymes that use the energy derived from an electrochemical proton gradient for ATP formation. We incorporated double-labeled F(0)F(1)-...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb718

    authors： Diez M,Zimmermann B,Börsch M,König M,Schweinberger E,Steigmiller S,Reuter R,Felekyan S,Kudryavtsev V,Seidel CA,Gräber P

    更新日期：2004-02-01 00:00:00

  • T-cell receptor recognition of HLA-DQ2-gliadin complexes associated with celiac disease.

    abstract：:Celiac disease is a T cell-mediated disease induced by dietary gluten, a component of which is gliadin. 95% of individuals with celiac disease carry the HLA (human leukocyte antigen)-DQ2 locus. Here we determined the T-cell receptor (TCR) usage and fine specificity of patient-derived T-cell clones specific for two epi...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2817

    authors： Petersen J,Montserrat V,Mujico JR,Loh KL,Beringer DX,van Lummel M,Thompson A,Mearin ML,Schweizer J,Kooy-Winkelaar Y,van Bergen J,Drijfhout JW,Kan WT,La Gruta NL,Anderson RP,Reid HH,Koning F,Rossjohn J

    更新日期：2014-05-01 00:00:00

  • Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus.

    abstract：:Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatch...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1823

    authors： Liu X,Zhang Q,Murata K,Baker ML,Sullivan MB,Fu C,Dougherty MT,Schmid MF,Osburne MS,Chisholm SW,Chiu W

    更新日期：2010-07-01 00:00:00

  • The opening of the two pores of the Hv1 voltage-gated proton channel is tuned by cooperativity.

    abstract：:In voltage-gated sodium, potassium and calcium channels, the functions of ion conduction and voltage sensing are performed by two distinct structural units: the pore domain and the voltage-sensing domain (VSD). In the hydrogen voltage-gated channel 1 (Hv1), the VSD, unusually, performs both functions. Hv1 was recently...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1738

    authors： Tombola F,Ulbrich MH,Kohout SC,Isacoff EY

    更新日期：2010-01-01 00:00:00

  • Messenger RNA interactions in the decoding center control the rate of translocation.

    abstract：:During protein synthesis, mRNA and tRNAs are iteratively translocated by the ribosome. Precisely what molecular event is rate limiting for translocation is not known. Here we show that disruption of the interactions between the A-site codon and the ribosome accelerates translocation, suggesting that the release of the...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2140

    authors： Khade PK,Joseph S

    更新日期：2011-10-23 00:00:00

  • Xist RNA antagonizes the SWI/SNF chromatin remodeler BRG1 on the inactive X chromosome.

    abstract：:The noncoding RNA Xist recruits silencing factors to the inactive X chromosome (Xi) and facilitates re-organization of Xi structure. Here, we examine the mouse epigenomic landscape of Xi and assess how Xist alters chromatin accessibility. Xist deletion triggers a gain of accessibility of select chromatin regions that ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-018-0176-8

    authors： Jégu T,Blum R,Cochrane JC,Yang L,Wang CY,Gilles ME,Colognori D,Szanto A,Marr SK,Kingston RE,Lee JT

    更新日期：2019-02-01 00:00:00

  • Nonsense-mediated mRNA decay occurs during eIF4F-dependent translation in human cells.

    abstract：:The nonsense-mediated mRNA decay (NMD) pathway degrades mRNAs undergoing premature termination of translation. It has been argued that in human cells, NMD is restricted to a pioneer round of translation initiated on mRNAs associated with the cap-binding complex (CBC) and that the exchange of the CBC for the eIF4F tran...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2575

    authors： Durand S,Lykke-Andersen J

    更新日期：2013-06-01 00:00:00

  • Crystal structure of the ribosome recycling factor bound to the ribosome.

    abstract：:In bacteria, disassembly of the ribosome at the end of translation is facilitated by an essential protein factor termed ribosome recycling factor (RRF), which works in concert with elongation factor G. Here we describe the crystal structure of the Thermus thermophilus RRF bound to a 70S ribosomal complex containing a ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1282

    authors： Weixlbaumer A,Petry S,Dunham CM,Selmer M,Kelley AC,Ramakrishnan V

    更新日期：2007-08-01 00:00:00

  • Evidence for a group II intron-like catalytic triplex in the spliceosome.

    abstract：:To catalyze pre-mRNA splicing, U6 small nuclear RNA positions two metals that interact directly with the scissile phosphates. U6 metal ligands correspond stereospecifically to metal ligands within the catalytic domain V of a group II self-splicing intron. Domain V ligands are organized by base-triple interactions, whi...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2815

    authors： Fica SM,Mefford MA,Piccirilli JA,Staley JP

    更新日期：2014-05-01 00:00:00

  • Evolutionary conservation of codon optimality reveals hidden signatures of cotranslational folding.

    abstract：:The choice of codons can influence local translation kinetics during protein synthesis. Whether codon preference is linked to cotranslational regulation of polypeptide folding remains unclear. Here, we derive a revised translational efficiency scale that incorporates the competition between tRNA supply and demand. App...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2466

    authors： Pechmann S,Frydman J

    更新日期：2013-02-01 00:00:00