Betula pendula Roth leaves: gastroprotective effects of an HPLC-fingerprinted methanolic extract.

Abstract:

:In this study, a methanolic extract of Betula pendula leaves (BLE) was investigated for its gastroprotective effects against 90% ethanol-induced ulcer in rats. Oral pretreatment of rats with BLE (100, 200 and 400 mg kg(- 1)) significantly reduced the incidence of gastric lesions induced by ethanol administration as compared with misoprostol (0.50 mg kg(- 1)). Furthermore, BLE inhibited the increase in malondialdehyde (MDA) and prevented depletion of total sulhydryl and non-protein sulhydryl groups in rat stomach homogenate when compared with ethanol group. With regard to the effect of lipid peroxidation in vitro, BLE showed the ability to reduce methyl linoleate autoxidation. Chemical characterisation of the main biologically active constituents of BLE was also achieved by means of high-performance liquid chromatography with photodiode array and mass spectrometry detection, showing the presence of myricetin-3-O-galactoside, quercetin glycosides, kaempferol glycosides.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Germanò MP,Cacciola F,Donato P,Dugo P,Certo G,D'Angelo V,Mondello L,Rapisarda A

doi

10.1080/14786419.2012.740036

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

1569-75

issue

17

eissn

1478-6419

issn

1478-6427

journal_volume

27

pub_type

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