Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography.

Abstract:

:Timosaponin AIII (TAIII) exhibits extensive pharmacological activities and has been reported as a potent antitumour agent for various human cancers. In the present study, a potential industrial process for producing TAIII that involves biotransformation directly in the crude extract liquid of rhizoma anemarrhenae (RA) was developed. β-D-glycosidase was used to transform timosaponin BII (TBII) into TAIII, and monofactor experiments were conducted to optimise the enzymolysis conditions. In addition, AB-8 macroporous resin column chromatography, preparative liquid chromatography, and crystallisation technique were applied for yielding TAIII crystals with a purity > 97%. Approximately, 7 g of TAIII with a high purity of > 97% was obtained from 1 kg of RA through this five-step preparation method, which can be used to produce TAIII on a large scale.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Lu L,Liu Y,Ding Y,Hou J,Zhang Y,Xue H,Zhang T

doi

10.1080/14786419.2016.1169416

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

2364-7

issue

20

eissn

1478-6419

issn

1478-6427

journal_volume

30

pub_type

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