Abstract:
:Timosaponin AIII (TAIII) exhibits extensive pharmacological activities and has been reported as a potent antitumour agent for various human cancers. In the present study, a potential industrial process for producing TAIII that involves biotransformation directly in the crude extract liquid of rhizoma anemarrhenae (RA) was developed. β-D-glycosidase was used to transform timosaponin BII (TBII) into TAIII, and monofactor experiments were conducted to optimise the enzymolysis conditions. In addition, AB-8 macroporous resin column chromatography, preparative liquid chromatography, and crystallisation technique were applied for yielding TAIII crystals with a purity > 97%. Approximately, 7 g of TAIII with a high purity of > 97% was obtained from 1 kg of RA through this five-step preparation method, which can be used to produce TAIII on a large scale.
journal_name
Nat Prod Resjournal_title
Natural product researchauthors
Lu L,Liu Y,Ding Y,Hou J,Zhang Y,Xue H,Zhang Tdoi
10.1080/14786419.2016.1169416subject
Has Abstractpub_date
2016-10-01 00:00:00pages
2364-7issue
20eissn
1478-6419issn
1478-6427journal_volume
30pub_type
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