Abstract：
:We investigated the telomere lengths of individual cell types in lingual mucosa using an improved tissue quantitative fluorescence in situ hybridization (Q-FISH) method. Our tissue Q-FISH method compensates for partially cut nuclei in a tissue section by using the telomere:centromere ratio (TCR). We normalized our TCR measurements (NTCR) using a section from a block of cultured cells placed on the same slide, thus improving the accuracy and reproducibility of the results. Normal lingual mucosa was obtained from 21 autopsied individuals. Immunohistochemistry showed positivity mainly for p27, p63, and CK19 in basal cells, and for Ki-67 in parabasal cells. Q-FISH revealed that NTCR was significantly highest in basal cells and lowest in prickle cells, and also that telomere length regressed at a certain rate in each cell type, firstly. Significant correlations of NTCR among the three epithelial cell types were demonstrated. The present findings appear to support the theory that stem cells exist in the basal layer of the lingual epithelium. The reduction of telomere length with age and in each cell layer is consistent with the telomere biology theory of cell proliferation and differentiation in oral mucosa.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Aida J,Izumiyama-Shimomura N,Nakamura K,Ishikawa N,Poon SS,Kammori M,Sawabe M,Arai T,Matsuura M,Fujiwara M,Kishimoto H,Takubo Kdoi
10.1016/j.exger.2008.06.001subject
Has Abstractpub_date
2008-09-01 00:00:00pages
833-9issue
9eissn
0531-5565issn
1873-6815pii
S0531-5565(08)00165-4journal_volume
43pub_type
杂志文章abstract：:Calorie restriction (CR) and a reduced growth hormone (GH) signal affect insulin sensitivity and lifespan in mammals in a similar manner. We investigated the effects of CR and moderate inhibition of GH on glucose-stimulated activation of insulin signaling and the expression of genes related to fat metabolism in white ...
journal_title：Experimental gerontology
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,随机对照试验
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更新日期：2016-06-15 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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更新日期：2007-05-01 00:00:00
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更新日期：2018-07-15 00:00:00
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doi：10.1016/j.exger.2012.04.001
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：Experimental gerontology
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更新日期：2013-12-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章
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更新日期：2014-09-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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更新日期：2005-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1988-01-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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更新日期：2019-06-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章
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更新日期：2012-12-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2014.06.017
更新日期：2014-11-01 00:00:00
abstract：:Increased lifespan can be associated with greater resistance to many different stressors, most notably thermal stress. Such hormetic effects have also been found in C. elegans where short-term exposure to heat lengthens the lifespan. Genetic investigations have been carried out using mutation perturbations in a single...
journal_title：Experimental gerontology
pub_type： 杂志文章
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