Induction of apoptosis accompanying with G(1) phase arrest and microtubule disassembly in human hepatoma cells by jaspolide B, a new isomalabaricane-type triterpene.


:Jaspolide B is a novel isomalabaricane-type triterpene isolated from sponge Jaspis sp. We investigated its effects on human hepatoma cells in this study. After 48 h of incubation, jaspolide B inhibited the growth of Bel-7402 and HepG2 cells with IC(50) values of 29.1 and 29.5 μM, respectively. Incubation with 0.5 μM of jaspolide B caused time-dependent induction of apoptosis in up to 66.8% of Bel-7402 cells for 48 h, and the induction of apoptosis was confirmed by the enhancement of mitochondrial masses and cell membrane permeability, and nuclear condensation in Bel-7402 and HepG2 cells. Moreover, jaspolide B arrested cell cycle progression at G(1) phase of human hepatoma cells in a dose- and time-dependent manner. In addition, treatment of the compound caused dose-dependent disassembly of microtubule cytoskeleton in Bel-7402 cells at indicated concentrations, and this effect being similar but weaker than that of colchicine, a well-known microtubule-disassembly agent. We conclude that the anti-cancer effect of jaspolide B relates to the apoptosis induction, cell cycle arrest and microtubule disassembly, and these multiple mechanisms of jaspolide B, especially the induction of apoptosis, open interesting perspectives for further exploration of the isomalabaricane-type terpenes and compounds of marine sponge origin as potential anticancer agents.


Cancer Lett


Cancer letters


Wei SY,Li M,Tang SA,Sun W,Xu B,Cui JR,Lin WH




Has Abstract


2008-04-08 00:00:00














  • Inhibitory effects of monoacylated 2-O-beta-galactosylglycerols on Epstein-Barr virus activation: the significant role of the hexanoyl chain.

    abstract::Three series of monoacyl-2-O-beta-D-galactosylglycerols bearing an acyl chain of varying length, from C4 to C10, were studied due to their antitumor promoting effects on the activation of the Epstein-Barr virus early antigen (EBV-EA), such activation being induced by the tumor promoter 12-O-tetradecanoylphorbol-13-ace...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Colombo D,Compostella F,Ronchetti F,Scala A,Toma L,Mukainaka T,Nagatsu A,Konoshima T,Tokuda H,Nishino H

    更新日期:1999-08-23 00:00:00

  • 4-Methyl-3-nitro-benzoic acid, a migration inhibitor, prevents breast cancer metastasis in SCID mice.

    abstract::Metastasis remains a formidable problem in malignant tumors. In this study, MTT assay revealed that 4-methyl-3-nitro-benzoic acid (MNBA) had no effect on cell viability and did not interfere with cell cycle in any breast cancer cell lines tested. However, treatment with MNBA on breast cancer cells can inhibit EGF-indu...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Guo H,Li M,Chen P,Blake DJ,Kong X,Hao X,Niu R,Zhang N

    更新日期:2011-06-01 00:00:00

  • Accumulation of cytoplasmic CDC25A in cutaneous squamous cell carcinoma leads to a dependency on CDC25A for cancer cell survival and tumor growth.

    abstract::Despite its documented role in cell cycle regulation, over-expression of the cyclin-dependent kinase activator CDC25A does not consistently correlate with worse cancer patient outcomes or predict successful clinical response to CDC25A inhibition. The current study was undertaken to investigate CDC25A in skin cancer an...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Al-Matouq J,Holmes T,Hammiller B,Tran N,Holmes M,Freeman SC,Hansen LA

    更新日期:2017-12-01 00:00:00

  • Cells of origin and tumor-initiating cells for nonmelanoma skin cancers.

    abstract::The epidermis of the skin is a multilayered stratified epithelium whose primary function is to provide a barrier against our external environment. As a result, cells in the epidermis are subject to constant assault from environmental pathogens, many of which can cause deleterious mutations. However, most of these muta...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Thieu K,Ruiz ME,Owens DM

    更新日期:2013-09-10 00:00:00

  • The missense mutations in the candidate prostate cancer gene ELAC2 do not alter enzymatic properties of its product.

    abstract::The candidate prostate cancer gene ELAC2 encodes tRNA 3' processing endoribonuclease (tRNase ZL). We produced recombinant human tRNase ZL's, which contain one to three amino-acid substitutions from three missense mutations (Ser217Leu, Ala541Thr, and Arg781His) that are associated with the occurrence of prostate cancer...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Minagawa A,Takaku H,Takagi M,Nashimoto M

    更新日期:2005-05-26 00:00:00

  • MCF-7 breast cancer cell line grown in agarose culture for study of COX-2 inhibitors in three-dimensional growth system.

    abstract::Herein we report a method for studying slow acting pharmaceutical COX-2 inhibitors in the MCF-7 breast cancer cell line where cells are grown in a three-dimensional format within an agarose matrix. The cancer cells are suspended in agarose and plated in a cell culture dish, where they will form small multicellular 'tu...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Kinder DH,Aulthouse AL

    更新日期:2004-03-08 00:00:00

  • Tbx3 expression is related to apoptosis and cell proliferation in rat bladder both hyperplastic epithelial cells and carcinoma cells.

    abstract::In the present study, Tbx3, a member of the T-box family of transcription factors, was identified as an up-regulated gene by mRNA differential display in the regression (apoptosis) stage after uracil-induction of papillomatosis in the rat urinary bladder. Immunohistochemical analysis revealed that apoptosis cells are ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Ito A,Asamoto M,Hokaiwado N,Takahashi S,Shirai T

    更新日期:2005-02-28 00:00:00

  • Down-regulation of protein kinase Ceta by antisense oligonucleotides sensitises A549 lung cancer cells to vincristine and paclitaxel.

    abstract::Previous studies point to protein kinase C (PKC) isozyme eta as a resistance factor in cancer cells. Therefore, we investigated whether down-regulation of PKCeta with second generation antisense oligonucleotides (ODNs) would sensitise A549 human lung carcinoma cells to cytostatics. The effects were compared to the out...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Sonnemann J,Gekeler V,Ahlbrecht K,Brischwein K,Liu C,Bader P,Müller C,Niethammer D,Beck JF

    更新日期:2004-06-25 00:00:00

  • Anti-tumor-promoting effects of phenylpropanoids on Epstein-Barr virus activation and two-stage mouse skin carcinogenesis.

    abstract::In our joint project in the search for anti-tumor promoters from natural plant sources, we carried out a primary screening of 12 phenylpropanoids isolated from Boronia pinnata Sm. (Rutaceae) by examining their possible inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradeca...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Ito C,Itoigawa M,Furukawa H,Ichiishi E,Mukainaka T,Okuda M,Ogata M,Tokuda H,Nishino H

    更新日期:1999-07-19 00:00:00

  • Hexachlorophene suppresses beta-catenin expression by up-regulation of Siah-1 in EBV-infected B lymphoma cells.

    abstract::Many studies have shown that the activation of beta-catenin signaling can promote oncogenesis, and it is therefore of interest to find agents that modulate this pathway. Recent work has shown using B lymphoma cells that infection by Epstein-Barr virus (EBV) and expression of its latent membrane protein (LMP)-1, cause ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Min HJ,Cho IR,Srisuttee R,Park EH,Cho DH,Ahn JH,Lee IS,Johnston RN,Oh S,Chung YH

    更新日期:2009-04-18 00:00:00

  • A case study in misidentification of cancer cell lines: MCF-7/AdrR cells (re-designated NCI/ADR-RES) are derived from OVCAR-8 human ovarian carcinoma cells.

    abstract::Multidrug-resistant MCF-7 breast adenocarcinoma cells (originally named MCF-7/AdrR cells and later re-designated NCI/ADR-RES) have served as an important and widely used research tool during the last two decades. However, the real identity of these cells has been in doubt since 1998 and has since been debated. The ori...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Liscovitch M,Ravid D

    更新日期:2007-01-08 00:00:00

  • Gastrointestinal cancers: influence of gut microbiota, probiotics and prebiotics.

    abstract::Cancers of the gastrointestinal (GI) tract continue to represent a major health problem, despite progress in therapy. Gut microbiota is a key element related to the genesis of GI cancers, countless papers addressing this burning issue across the world. We provide an updated knowledge of the involvement of gut microbio...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Serban DE

    更新日期:2014-04-10 00:00:00

  • Mistletoe lectins I, II and III induce the production of cytokines by cultured human monocytes.

    abstract::The three mistletoe (Viscum album L.) lectins. ML I, ML II and ML III, were tested on their ability to enhance the secretion of the cytokines tumor necrosis factor (TNF)alpha, interleukin (IL)-1 alpha, IL-1 beta and IL-6 by human monocytes obtained from healthy donors. At lectin concentrations from 0.02 to 20/pg ml (1...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Ribéreau-Gayon G,Dumont S,Muller C,Jung ML,Poindron P,Anton R

    更新日期:1996-12-03 00:00:00

  • Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies.

    abstract::Monoclonal antibodies were produced and characterized in order to allow the monitoring of the urinary excretion of six modified nucleosides. The specificity of each antibody was determined and competitive solid-phase enzyme-linked immunoassays were designed, the sensitivity of which lay in the pmol range. Detection an...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Reynaud C,Bruno C,Boullanger P,Grange J,Barbesti S,Niveleau A

    更新日期:1992-01-31 00:00:00

  • Antitumor activity of nanoliposomes encapsulating the novobiocin analog 6BrCaQ in a triple-negative breast cancer model in mice.

    abstract::In this study, we investigated the anticancer efficacy of pegylated liposomes containing 6BrCaQ, an hsp90 inhibitor derived from novobiocin. 6BrCaQ has been previously identified as the most potent compound in a series of quinoleic novobiocin analogs but is poorly water-soluble. We investigated, for the first time, th...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Sauvage F,Fattal E,Al-Shaer W,Denis S,Brotin E,Denoyelle C,Blanc-Fournier C,Toussaint B,Messaoudi S,Alami M,Barratt G,Vergnaud-Gauduchon J

    更新日期:2018-09-28 00:00:00

  • Induction of cytogenetic adaptive response of mouse bone marrow cells to radiation by therapeutic doses of bleomycin sulfate and actinomycin D as assayed by the micronucleus test.

    abstract::An in vivo micronucleus assay using bone marrow cells of Syrian albino male mice for identifying the possibility of induction of adaptive response to various doses of radiation following treatment with chemotherapeutics is described. Single doses of bleomycin sulfate (BLM-S) at 300 micrograms/kg and actinomycin-D (ACT...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Mozdarani H,Saberi AH

    更新日期:1994-04-01 00:00:00

  • Marked elevation of hypusine formation activity on eukaryotic initiation factor 5A in v-HA-RAS transformed mouse NIH3T3 cells.

    abstract::Hypusine formation on the eukaryotic initiation factor 5A (eIF-5A) precursor is ubiquitously present in eukaryotic cells and archebacteria. In this reaction, deoxyhypusine synthase catalyzes the conversion of one unique lysine residue on eIF-5A to deoxyhypusine using spermidine as the substrate. Hydroxylation of the d...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Chen ZP,Chen KY

    更新日期:1997-05-19 00:00:00

  • An enzyme immunoassay for the detection of human tumor nucleolar antigens.

    abstract::An enzyme immunoassay (EIA) was developed in which nuclear extracts were bound to the wells of polystyrene microtiter plates. The presence of various antigens in the extracts could be detected using antiserum raised against HeLa cell nucleoli. Normal human cell extracts, coupled to Sepharose as a solid phase absorbent...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Kelsey DE,Busch RK,Busch H

    更新日期:1981-05-01 00:00:00

  • Abnormalities of retinoblastoma gene structure in human lung tumors.

    abstract::The retinoblastoma (Rb) gene is associated with the pathogenesis of several types of human cancer, including retinoblastoma, osteosarcoma, soft tissue sarcomas, and lung, breast and bladder carcinomas. Loss of heterozygosity is a common mode in allelic inactivation of Rb and other tumor-suppressor genes. We investigat...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Linardopoulos S,Gonos ES,Spandidos DA

    更新日期:1993-07-30 00:00:00

  • Exosomes in hypoxia-induced remodeling of the tumor microenvironment.

    abstract::Exosomes are structurally and functionally pleiotropic nano-sized (~30-150 nm in diameter) extracellular vesicles (EVs) with endosomal origin. These vesicles are secreted by almost all cells and play a significant role in intercellular communication and bio-waste disposal. To a great extent, exosomes represent biologi...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Kumar A,Deep G

    更新日期:2020-09-28 00:00:00

  • Anti-EGFR and ErbB-2 antibodies attenuate cyclooxygenase-2 expression and cooperatively inhibit survival of human colon cancer cells.

    abstract::Cyclooxygenase-2 (COX-2) is a transcriptional target and downstream effector of the ErbB-1 (EGFR) and ErbB-2 signaling pathways. We found that anti-EGFR and anti-ErbB-2 antibodies inhibited ERK phosphorylation and downregulated COX-2 protein expression in HCA-7 human colon carcinoma cells. Both antibodies also augment...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Half E,Sun Y,Sinicrope FA

    更新日期:2007-06-28 00:00:00

  • Identification of LIMK2 as a therapeutic target in castration resistant prostate cancer.

    abstract::This study identified LIMK2 kinase as a disease-specific target in castration resistant prostate cancer (CRPC) pathogenesis, which is upregulated in response to androgen deprivation therapy, the current standard of treatment for prostate cancer. Surgical castration increases LIMK2 expression in mouse prostates due to ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Nikhil K,Chang L,Viccaro K,Jacobsen M,McGuire C,Satapathy SR,Tandiary M,Broman MM,Cresswell G,He YJ,Sandusky GE,Ratliff TL,Chowdhury D,Shah K

    更新日期:2019-04-28 00:00:00

  • Dietary ortho phenols that induce glutathione S-transferase and increase the resistance of cells to hydrogen peroxide are potential cancer chemopreventives that act by two mechanisms: the alleviation of oxidative stress and the detoxification of mutagenic

    abstract::Oxidative stress is implicated in the etiology of cancer, hence compounds that alleviate oxidative stress by inducing enzymes that defend against free radical damage might be useful as cancer chemopreventives. Glutathione S-transferase (GST) has been suggested to be a candidate for a critical enzyme in protecting cell...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Fiander H,Schneider H

    更新日期:2000-08-11 00:00:00

  • Insulin-like growth factor binding protein-3 inhibits migration of endometrial cancer cells.

    abstract::Cell migration and invasion leading to metastasis is a major cause of death from endometrial cancer (EC). We have shown that the rate of EC cell migration is inversely related to the level of insulin-like growth factor protein-3 (IGFBP-3). Down-regulation of IGFBP-3 by siRNA in EC cells accelerated migration without a...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Gribben L,Baxter RC,Marsh DJ

    更新日期:2012-04-01 00:00:00

  • Quercetin exerts a preferential cytotoxic effect on active dividing colon carcinoma HT29 and Caco-2 cells.

    abstract::The effect of the naturally occurring flavonol, quercetin, was investigated on cell growth and metabolism of two human carcinoma cell lines, HT29 and Caco-2 cells, both during the exponentially growing phase and after confluence. Our results show clearly that, after a 48-h period of treatment, quercetin (in the range ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Agullo G,Gamet L,Besson C,Demigné C,Rémésy C

    更新日期:1994-11-25 00:00:00

  • Growth inhibition of a prostate tumor by alpha-difluoromethylornithine and by cyclophosphamide.

    abstract::The effects of the ornithine decarboxylase suicide substrate, difluoromethylornithine (DFMO), and of cyclophosphamide, individually and in combination, on the growth of the R3327MAT-Lu prostate derived tumor were determined. DFMO decreased the growth rate and resulted in a 75% reduction in DNA content compared to the ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Heston WD,Kadmon D,Fair WR

    更新日期:1982-05-01 00:00:00

  • Mitofusin-2 triggers mitochondria Ca2+ influx from the endoplasmic reticulum to induce apoptosis in hepatocellular carcinoma cells.

    abstract::In previous studies, we confirmed that mitofusin-2 (Mfn2) induced apoptosis in hepatocellular carcinoma (HCC) cells. However, the exact molecular mechanism remained unclear. Mfn2 expressed lower in tumour tissues, compared with adjacent non-cancer tissues. Furthermore, Mfn2 immunostaining was very weak in HCC tissue (...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Wang W,Xie Q,Zhou X,Yao J,Zhu X,Huang P,Zhang L,Wei J,Xie H,Zhou L,Zheng S

    更新日期:2015-03-01 00:00:00

  • Exosome-encapsulated miRNAs contribute to CXCL12/CXCR4-induced liver metastasis of colorectal cancer by enhancing M2 polarization of macrophages.

    abstract::Tumor-associated macrophages (TAMs) are important immunocytes associated with cancer metastasis. However, whether TAMs play a dominant role in mediating CXCL12/CXCR4-induced liver metastasis of colorectal cancer (CRC) remains unexplored. Herein, we found that CD206+ TAMs, which infiltrated at the invasive front, were ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Wang D,Wang X,Si M,Yang J,Sun S,Wu H,Cui S,Qu X,Yu X

    更新日期:2020-04-01 00:00:00

  • MUC16 C terminal-induced secretion of tumor-derived IL-6 contributes to tumor-associated Treg enrichment in pancreatic cancer.

    abstract::Pancreatic cancer is the most lethal tumor. CA125 (gene symbol MUC16) is an important serum marker for pancreatic cancer diagnosis and treatment. High serum CA125 is related to metabolic tumor burden and poor prognosis. The circulating Treg subset is another independent prognostic factor for pancreatic cancer. Our unp...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Fan K,Yang C,Fan Z,Huang Q,Zhang Y,Cheng H,Jin K,Lu Y,Wang Z,Luo G,Yu X,Liu C

    更新日期:2018-04-01 00:00:00

  • Changes in insulin-like growth factor-I receptor expression and binding protein secretion associated with tamoxifen resistance and estrogen independence in human breast cancer cells in vitro.

    abstract::Conditioned medium from a series of human breast cancer cell lines representing the phenotypes of estrogen dependence ZR-75-1, MCF-7), tamoxifen resistance (Z(+)-75-9a1, LY2) and estrogen independence (ZR-PR-Lt) contained four detectable insulin-like growth factor binding proteins (IGFBPs) of MW 24, 34, 44 and 70 kDa....

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: McCotter D,van den Berg HW,Boylan M,McKibben B

    更新日期:1996-02-06 00:00:00