Carcinogenesis by methylbenzylnitrosamine near the squamocolumnar junction and methylamylnitrosamine metabolism in the mouse forestomach.


:We repeated and extended a 1973 study by Sander and Schweinsberg on forestomach tumorigenesis in mice by methylbenzylnitrosamine (MBZN). Groups of 80 adult CD-1 mice of both sexes received 96 mg/kg of MBZN subdivided into 24 doses of 4 mg/kg, 12 doses of 8 mg/kg or 6 doses of 16 mg/kg (groups 1-3, respectively). The mice were injected i.p. twice weekly with MBZN in 30% dimethylsulfoxide and 6-8 mice/group were killed every 4 weeks up to 40 weeks. Ten untreated control mice did not develop forestomach tumors. Forestomach papillomas occurred in 35-53% of the treated mice, with the highest incidence and shortest latency (mostly <24 weeks) in group 3. Squamous carcinomas of the forestomach were found in 31% of group 1 and 4-6% of groups 2 and 3. Ninety-two percent of the carcinomas and 94% of the papillomas in the 8-mm wide forestomach occurred < or = 1 mm from the squamocolumnar junction (SCJ) with the glandular stomach. This is interesting in view of the rising incidence of human adenocarcinoma near the gastroesophageal SCJ. Methyl-n-amylnitrosamine (MNAN) yields 2-, 3- and 4-hydroxy-MNAN (HO-MNAN) in a 1:3:2 ratio when incubated with rodent tissues for which MNAN is carcinogenic. This metabolism may be due to cytochrome P450 isoform believed responsible for MNAN and, probably, MBZN activation. When freshly excised mouse forestomach and esophagus were incubated for 2 h with 23 microM MNAN, total HO-MNAN yields were 0.79 +/- 0.05 and 1.81 +/-0.08 nmol/100 mg tissue per h (mean +/- SE), respectively, with about 1:3:2 ratios between 2-,3- and 4-HO-MNAN. This compares with published mean HO-MNAN yields in nmol/100 mg per h of 1.2 for rat esophagus (where MNAN and MBZN are strongly carcinogenic) and <0.1 for rat forestomach. These findings may explain why MNAN and MBZN induce forestomach tumors in mice but not in rats and why MNAN induces esophageal tumors in mice, but does not explain why MBZN given i.p. fails to induce esophageal tumors in mice. Three sections of the mouse forestomach (closest-to to furthest-from the SCG) showed total HO-MNAN yields from MNAN of 0.61+/-0.05, 0.38+/-0.03 and 0.48+/-0.02 nmol/100 mg per h (mean +/-SE), respectively. This may help explain why the MBZN-induced forestomach tumors were non-centrated near the SCJ.


Cancer Lett


Cancer letters


Schneider P,Hinrichs S,Zulim R,Towery R,Morris C,Mirvish SS




Has Abstract


1996-04-19 00:00:00














  • Diurnal variation in the response of gamma-ray-induced apoptosis in the mouse intestinal epithelium.

    abstract::The appearance of apoptosis has been used as an index of carcinogen-induced damage incurred by the intestinal epithelium. Here we report that the cytotoxic response elicited by gamma radiation in the duodenal and colonic epithelium of the mouse is markedly affected by the time of day at which the animals are irradiate...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Duncan AM,Ronen A,Blakey DH

    更新日期:1983-12-01 00:00:00

  • Mutations in the VHL gene from potassium bromate-induced rat clear cell renal tumors.

    abstract::Potassium bromate (KBrO(3)) is a rat renal carcinogen and a major drinking water disinfection by-product in water disinfected with ozone. Clear cell renal tumors, the most common form of human renal epithelial neoplasm, are rare in animals but are inducible by KBrO(3) in F344 rats. Detection of cytoplasmic periodic ac...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Shiao YH,Kamata SI,Li LM,Hooth MJ,DeAngelo AB,Anderson LM,Wolf DC

    更新日期:2002-12-10 00:00:00

  • NRF2 antioxidant response protects against acidic bile salts-induced oxidative stress and DNA damage in esophageal cells.

    abstract::Gastroesophageal reflux disease (GERD) is the main risk factor for Barrett's tumorigenesis. In this study, we investigated the role of NRF2 in response to exposure to acidic bile salts (ABS), in conditions that mimic GERD, using Barrett's esophagus cell models. We detected an increase in NRF2 protein levels, following...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Peng D,Lu H,Zhu S,Zhou Z,Hu T,Chen Z,Zaika A,El-Rifai W

    更新日期:2019-08-28 00:00:00

  • Effects of processing conditions on glucosinolates in cruciferous vegetables.

    abstract::Glucosinolates constitute a well-defined group of secondary plant metabolites in cruciferous plants. They occur especially in brassica vegetables, which represent a major part of the human diet. Glucosinolates undergo hydrolysis, catalysed by an endogenous plant enzyme, known as myrosinase, into a range of biological ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Verkerk R,van der Gaag MS,Dekker M,Jongen WM

    更新日期:1997-03-19 00:00:00

  • Nitric oxide as a target of complementary and alternative medicines to prevent and treat inflammation and cancer.

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    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Hofseth LJ

    更新日期:2008-09-08 00:00:00

  • A redox ruthenium compound directly targets PHD2 and inhibits the HIF1 pathway to reduce tumor angiogenesis independently of p53.

    abstract::Targeting specific tumor metabolic needs represents an actively investigated therapeutic strategy to bypass tumor resistance mechanisms. In this study, we describe an original approach to impact the cancer metabolism by exploiting the redox properties of a ruthenium organometallic compound. This organometallic complex...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Vidimar V,Licona C,Cerón-Camacho R,Guerin E,Coliat P,Venkatasamy A,Ali M,Guenot D,Le Lagadec R,Jung AC,Freund JN,Pfeffer M,Mellitzer G,Sava G,Gaiddon C

    更新日期:2019-01-01 00:00:00

  • Genetic damage and the inhibition of 7,12-dimethylbenz[a]anthracene-induced genetic damage by the phytoestrogens, genistein and daidzein, in female ICR mice.

    abstract::Populations consuming soybeans have reduced rates of breast, colon and prostate cancer possibly due, in part, to the presence in soybeans of two estrogenic isoflavones, genistein and daidzein. This study investigated the genotoxicity of these soya isoflavones and their interactions with 7,12-dimethylbenz[a]anthracene ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Giri AK,Lu LJ

    更新日期:1995-08-16 00:00:00

  • The XPD Asp312Asn and Lys751Gln polymorphisms, corresponding haplotype, and pancreatic cancer risk.

    abstract::We evaluated the association between the XPD exon 10 Asp(312)Asn and exon 23 Lys(751)Gln polymorphisms and the risk of pancreatic cancer in a hospital-based study of 344 patients and 386 controls frequency matched by age, gender, and race. Stratified analyses showed ever smokers carrying the Asn(312)Asn genotype had a...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Jiao L,Hassan MM,Bondy ML,Abbruzzese JL,Evans DB,Li D

    更新日期:2007-01-08 00:00:00

  • SOX7, down-regulated in colorectal cancer, induces apoptosis and inhibits proliferation of colorectal cancer cells.

    abstract::The sex-determining region Y-box 7 (Sox7) is a member of high mobility group (HMG) transcription factor family, essential for embryonic development and endoderm differentiation. Deregulation of Wnt signaling pathway is a hallmark of colorectal cancer. Our results showed that the expression level of SOX7 was frequently...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Zhang Y,Huang S,Dong W,Li L,Feng Y,Pan L,Han Z,Wang X,Ren G,Su D,Huang B,Lu J

    更新日期:2009-05-08 00:00:00

  • Increased sensitivity to indomethacin of mice bearing the BCL1-leukemia.

    abstract::Indomethacin is a non-steroidal anti-inflammatory agent that inhibits prostaglandin synthesis. Administration of indomethacin, in doses which were non-toxic to normal BALB/c mice, to mice bearing the BCL1 leukemia resulted in increased mortality of these animals. This effect was only observed if the indomethacin was a...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Hart DA

    更新日期:1984-06-01 00:00:00

  • Activation of fibronectin/PI-3K/Akt2 leads to chemoresistance to docetaxel by regulating survivin protein expression in ovarian and breast cancer cells.

    abstract::The purpose of this study was to investigate the possible role of PI-3K/Akt2 pathway in docetaxel-induced apoptosis. Here we showed that transfection of full-length Akt2 into breast and ovarian cancer cells could provoke Akt phosphorylation and induce an enhanced resistance to docetaxel. FN adhesion promoted Akt phosp...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Xing H,Weng D,Chen G,Tao W,Zhu T,Yang X,Meng L,Wang S,Lu Y,Ma D

    更新日期:2008-03-08 00:00:00

  • Melatonin down-regulates hTERT expression induced by either natural estrogens (17beta-estradiol) or metalloestrogens (cadmium) in MCF-7 human breast cancer cells.

    abstract::The goal was to evaluate whether melatonin (Mel) down-regulates hTERT expression induced by 17beta-estradiol (E(2)) or cadmium (Cd) in breast cancer cells. We found that: (a) Mel inhibits E(2) or Cd-induced hTERT transcription in hTERT-Luc transfected MCF-7 cells, (b) Mel significantly reduces E(2)- and Cd-mediated hT...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Martínez-Campa CM,Alonso-González C,Mediavilla MD,Cos S,González A,Sanchez-Barcelo EJ

    更新日期:2008-09-18 00:00:00

  • The role of membrane transporters in drug delivery to brain tumors.

    abstract::Most brain tumors are highly resistant to chemotherapy because many chemotherapeutic drugs poorly cross the blood-brain barrier, the blood-cerebrospinal-fluid barrier, and the plasma membrane of the tumor cells. This restricted drug delivery is largely due to the presence of integral plasma membrane proteins belonging...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Nies AT

    更新日期:2007-08-28 00:00:00

  • Metronomics in the neoadjuvant and adjuvant treatment of breast cancer.

    abstract::The concept of metronomic chemotherapy (MC) has evolved from a descriptive preclinical phenomenon encompassing inhibition of angiogenesis to a clinically validated treatment concept involving multiple potential mechanisms of action. Clinicians are progressively more incline to consider MC as a component of mainstream ...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Munzone E,Colleoni M

    更新日期:2017-08-01 00:00:00

  • Detection of a novel lactate dehydrogenase isozyme and an apparent differentiation-associated shift in isozyme profile in hepatoma cell lines.

    abstract::A hitherto unreported lactate dehydrogenase (LD) isoenzyme, which migrates electrophoretically to the relative position between LD2 and LD3 has been identified in the electropheratogram in 7 of 7 (100%) cultured hepatoma cell lines with various degrees of differentiation and is thus given the name LD2-3. LD2-3 seems t...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Liu TZ,Chen PY,Chiu DT,Wei JS,Chang KS,Lin KH

    更新日期:1994-12-09 00:00:00

  • alpha-Toxin of Staphylococcus aureus overcomes acquired cisplatin-resistance in malignant mesothelioma cells.

    abstract::alpha-Toxin (alpha-hemolysin) of Staphylococcus aureus is a pore-forming bacterial toxin which after caveolin-1-dependent assembly induces apoptosis in eukaryotic cells. We investigated if a sub-toxic concentration of staphylococcal alpha-toxin could enhance cisplatin-induced apoptosis and overcome acquired cisplatin-...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Johansson D,Johansson A,Behnam-Motlagh P

    更新日期:2008-06-28 00:00:00

  • Activation of signal transduction pathways during hepatic oncogenesis.

    abstract:BACKGROUND AND AIMS:Understanding the molecular pathogenesis of hepatocellular carcinoma (HCC) is essential to identify therapeutic targets. A hepatitis B virus (HBV) related double transgenic murine model was developed. METHODS:Liver specific expression of HBV X protein (HBx) and insulin receptor substrate 1 (IRS1) w...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Chung W,Kim M,de la Monte S,Longato L,Carlson R,Slagle BL,Dong X,Wands JR

    更新日期:2016-01-01 00:00:00

  • Induction of apoptosis in human lymphocytes treated with Viscum album L. is mediated by the mistletoe lectins.

    abstract::Viscum album L. (VAL) is a phytopreparation used in adjuvant cancer therapy with both immunostimulatory and DNA stabilizing properties at low drug concentrations and cytostatic/cytotoxic properties at higher concentrations. The present work examines the cytotoxic effects of VAL extracts produced from mistletoes grown ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Büssing A,Suzart K,Bergmann J,Pfüller U,Schietzel M,Schweizer K

    更新日期:1996-01-19 00:00:00

  • The proliferative response of HT-29 human colon adenocarcinoma cells to bombesin-like peptides.

    abstract::Bombesin-like peptides (BLP) and their receptors are widely distributed throughout the intestine and are potential mitogens for gastrointestinal cancers. In this study we characterized the proliferation induced by BLP in the human adenocarcinoma cell line HT-29. The number of HT-29 cells, partially serum deprived (1% ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Cassano G,Resta N,Gasparre G,Lippe C,Guanti G

    更新日期:2001-10-30 00:00:00

  • Application of CRISPR-mediated genome engineering in cancer research.

    abstract::Cancer is a multistep process that arises from a series of genetic and epigenetic events. With recent technological advances there has been a burst in genome sequencing and epigenetic studies revealing a plethora of alterations that may contribute to cancer. However, the great challenge for the cancer research communi...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Sayin VI,Papagiannakopoulos T

    更新日期:2017-02-28 00:00:00

  • Glutathione peroxidase and glutathione reductase activities in cancerous and non-cancerous human kidney tissues.

    abstract::Selenium-dependent (Se-GSH-Px), selenium-independent (non-Se-GSH-Px) glutathione peroxidase and glutathione reductase (GSSG-Rx) activities have been determined in cancerous and non-cancerous human adult kidney. Large inter-individual variation in the activities of all enzymes tested were found in both tumour and non-t...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Di Ilio C,Sacchetta P,Angelucci S,Zezza A,Tenaglia R,Aceto A

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  • Stress-induced upregulation of TNFSF4 in cancer-associated fibroblast facilitates chemoresistance of lung adenocarcinoma through inhibiting apoptosis of tumor cells.

    abstract::Stress conditions induced by routine treatments might affect cancer-associated fibroblasts in lung adenocarcinoma. The present study tried to explore transcriptome changes in lung fibroblasts under chemotherapeutics, irradiation, and hypoxia, which were induced by chemotherapy, radiotherapy, and anti-angiogenesis ther...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Li Y,Chen Y,Miao L,Wang Y,Yu M,Yan X,Zhao Q,Cai H,Xiao Y,Huang G

    更新日期:2021-01-28 00:00:00

  • Cytotoxic effect of butein on human colon adenocarcinoma cell proliferation.

    abstract::Several classes of plant polyphenols namely, flavonoids, chalcones and coumarins exhibited varying degrees of inhibition on the cell proliferation of human colon adenocarcinoma cell line 220.1. At the highest concentration tested (100 microM), many of the chalcones showed > 100% growth inhibition and their order of po...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yit CC,Das NP

    更新日期:1994-07-15 00:00:00

  • Transductional and transcriptional targeting of cancer cells using genetically engineered viral vectors.

    abstract::Gene delivery vectors, including adenovirus (Ad) and adeno-associated virus (AAV), are inefficient and non-selective for cancer due to low levels of viral receptors with high levels on other tissues, including liver. We tested Ads and AAVs with the SIGYPLP-targeting peptide inserted into virus capsids for transduction...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Nicklin SA,Dishart KL,Buening H,Reynolds PN,Hallek M,Nemerow GR,Von Seggern DJ,Baker AH

    更新日期:2003-11-25 00:00:00

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    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Mannello F,Bocchiotti GD,Morano FP,Fratepietro LM,Gazzanelli G

    更新日期:1996-01-02 00:00:00

  • Cancer metabolism and mass spectrometry-based proteomics.

    abstract::Cancer metabolism has been extensively investigated by various tools, and the fact of diverse metabolic reprogramming in cancer cells has been gradually unveiled. In this review, we discuss some contributions in cancer metabolism by general proteomic analysis and post-translational modification analysis using mass spe...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Zhou W,Liotta LA,Petricoin EF

    更新日期:2015-01-28 00:00:00

  • Vascular mimicry formation is promoted by paracrine TGF-β and SDF1 of cancer-associated fibroblasts and inhibited by miR-101 in hepatocellular carcinoma.

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    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yang J,Lu Y,Lin YY,Zheng ZY,Fang JH,He S,Zhuang SM

    更新日期:2016-12-01 00:00:00

  • Competitive uptake of porphyrin and LDL via the LDL receptor in glioma cell lines: flow cytometric analysis.

    abstract::We examined the simultaneous uptake of porphyrin and (LDL) by four established cell lines of glioma and normal fibroblasts using flow cytometry (FCM). The results indicated porphyrin and LDL showed competitive conjugation with the LDL receptor. These results support the theory of the porphyrin uptake via the LDL recep...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Shibata Y,Matsumura A,Yoshida F,Yamamoto T,Nakai K,Nose T,Sakata I,Nakajima S

    更新日期:2001-05-10 00:00:00

  • Acute inhibition of DNA synthesis in hamster buccal pouch epithelium exposed to indirect acting carcinogens.

    abstract::Inhibition of DNA synthesis was observed and quantitated in hamster buccal pouch epithelium exposed in vivo and in vitro to indirect acting carcinogens. Topical application of a 0.5% solution of the potent hamster buccal pouch carcinogen 7,12-dimethylbenz[a]-anthracene (DMBA) acutely inhibited epithelial DNA synthesis...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Nagabhushan M,Ng YK,Elias R,Polverini PJ,Solt DB

    更新日期:1990-09-01 00:00:00

  • Effects of tyrosinase activity on the cytotoxicity of 4-S-cysteaminylphenol and N-acetyl-4-S-cysteaminylphenol in melanoma cells.

    abstract::The rationale for melanoma specific antitumor agents containing phenolic amines is based in part upon the ability of the enzyme tyrosinase to oxidize these prodrugs to toxic intermediates. Two phenolic amine compounds, N-acetyl-4-S-cysteaminylphenol (N-Ac-4-S-CAP) and 4-S-cysteaminylphenol (4-S-CAP), demonstrated grow...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Prezioso JA,Epperly MW,Wang N,Bloomer WD

    更新日期:1992-03-31 00:00:00