Abstract:
:Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression of ToxA, ToxB and Cpe fragments either as individual components or as a hybrid multidomain (ToxA-ToxB-Cpe) protein. Rabbit monospecific sera raised against individual peptides reacted with the chimeric product indicating that the corresponding antigenic determinants were correctly expressed on the hybrid molecule. Furthermore, mice immunized with the fusion protein produced antibodies specific to each of the three separate components. These data suggest that the constructed three-domain molecule could be used in future studies for development of a vaccine against enteric clostridial diseases.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Belyi IF,Varfolomeeva NAdoi
10.1016/S0378-1097(03)00560-3keywords:
subject
Has Abstractpub_date
2003-08-29 00:00:00pages
325-9issue
2eissn
0378-1097issn
1574-6968pii
S0378109703005603journal_volume
225pub_type
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