Serine racemase homologue of Saccharomyces cerevisiae has L-threo-3-hydroxyaspartate dehydratase activity.

abstract：:An investigation of mortality of piglets through clinical signs, post-mortem, histopathology and bacteriological analyses revealed the causal organism to be Escherichia coli, mainly O149:K91:K88 which belongs to the enterotoxigenic biotypes. Molecular characterization and epidemiologic analysis elucidated it as shiga-...

journal_title：FEMS microbiology letters

authors： Kanengoni AT,Thomas R,Gelaw AK,Madoroba E

更新日期：2017-02-01 00:00:00

abstract：:Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this prob...

journal_title：FEMS microbiology letters

authors： Zwirglmaier K

更新日期：2005-05-15 00:00:00

abstract：:Chromosomal DNA from 62 strains of 'Campylobacter upsaliensis' from blood and faecal specimens of patients in Australia, Belgium, South Africa and England, and seven dog isolates from England and Sweden were examined. DNA base compositions of 21 strains were 35 +/- 1 mol% G + C. Electrophoresis of HaeIII DNA digests g...

journal_title：FEMS microbiology letters

authors： Owen RJ,Hernandez J

更新日期：1990-10-01 00:00:00

abstract：:Digital game-based learning (DGBL) is being used increasingly as an alternative learning tool to teach science in further and higher education. A variety of digital game formats currently exist for science learning, alongside diverse methods for their implementation and evaluation. This paper aims to provide a broad s...

journal_title：FEMS microbiology letters

authors： Brown CL,Comunale MA,Wigdahl B,Urdaneta-Hartmann S

更新日期：2018-11-01 00:00:00

abstract：:We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the muta...

journal_title：FEMS microbiology letters

authors： Beck R,Crooke H,Jarsch M,Cole J,Burtscher H

更新日期：1994-12-01 00:00:00

abstract：:The mosquitocidal crystal protein, Cry19Aa, from Bacillus thuringiensis ssp. jegathesan, has high toxicity to Anopheles stephensi and Culex pipiens but is less toxic to Aedes aegypti. To study the functional role of putative domain II and surface residues in mosquito toxicity, 16 alanine substitution mutations were in...

journal_title：FEMS microbiology letters

authors： Roh JY,Nair MS,Liu XS,Dean DH

更新日期：2009-06-01 00:00:00

abstract：:The formation of dormant endospores is a complex morphological process that permits long-term survival in inhospitable environments for many Gram-positive bacteria. Sporulation for the anaerobic gastrointestinal pathogen Clostridium difficile is necessary for survival outside of the gastrointestinal tract of its host....

journal_title：FEMS microbiology letters

authors： Edwards AN,McBride SM

更新日期：2014-09-01 00:00:00

abstract：:The lin genes encode the gamma-hexachlorocyclohexane (gamma-HCH or lindane) catabolic pathway in lindane-degrading strains. The location and stability of these genes have been explored in the lindane-degrading Sphingobium francense strain Sp+, and in two non-lindane-degrading mutants (Sp1- and Sp2-). The lin genes, li...

journal_title：FEMS microbiology letters

authors： Cérémonie H,Boubakri H,Mavingui P,Simonet P,Vogel TM

更新日期：2006-04-01 00:00:00

abstract：:The Mfa1 protein of Porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between P. gingivalis and Streptococcus gordonii. We utilized a promoter-lacZ reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. Promoter acti...

journal_title：FEMS microbiology letters

authors： Park Y,James CE,Yoshimura F,Lamont RJ

更新日期：2006-09-01 00:00:00

abstract：:To rapidly identify natural isolates of marine bioluminescent bacteria, we developed amplified ribosomal DNA restriction analysis (ARDRA) methods. ARDRA, which is based on the restriction patterns of 16S rRNA gene digested with five enzymes (EcoRI, DdeI, HhaI, HinfI, RsaI), clearly distinguished the 14 species of mari...

journal_title：FEMS microbiology letters

authors： Kita-Tsukamoto K,Wada M,Yao K,Kamiya A,Yoshizawa S,Uchiyama N,Kogure K

更新日期：2006-03-01 00:00:00

abstract：:The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from t...

journal_title：FEMS microbiology letters

authors： Brans A,Loriaux A,Thamm I,Joris B,Dusart J

更新日期：1997-04-15 00:00:00

abstract：:The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD50 values in a murine infection model. Pig challenge...

journal_title：FEMS microbiology letters

authors： Tascón Cabrero RI,Vázquez-Boland JA,Gutiérrez CB,Rodríguez-Barbosa JI,Rodríguez-Ferri EF

更新日期：1997-03-01 00:00:00

abstract：:To clarify the cause of the supersusceptibility of Branhamella catarrhalis to macrolide antibiotics, which are well-known to be inactive to most Gram-negative bacteria, we determined its cell surface hydrophobicity by the partition experiment between water and hydrocarbons. Its cell surface was found to be markedly mo...

journal_title：FEMS microbiology letters

authors： Gotoh N,Tanaka S,Nishino T

更新日期：1989-05-01 00:00:00

abstract：:Salmonella Typhimurium harbors two Salmonella pathogenicity islands (SPIs), each encoding a type three secretion system for virulence proteins. Although there is increasing evidence of postinvasion roles for SPI-1, it has been generally accepted that SPI-1 genes are downregulated following the invasion process. Here, ...

journal_title：FEMS microbiology letters

authors： Giacomodonato MN,Sarnacki SH,Llana MN,Cerquetti MC

更新日期：2011-04-01 00:00:00

abstract：:Academic work at different career stages has changed and a broadened portfolio of expertise enables academics to adapt, maintain and advance their career. Development related to research activity is naturally driven by methodology and technology. Institutions and peers largely support development in the contexts of di...

journal_title：FEMS microbiology letters

authors： Fahnert B

更新日期：2015-10-01 00:00:00

abstract：:NadR is the repressor protein that controls the expression of genes for NAD synthesis. It is also believed to be involved in nucleotide transport. Point mutations conferring different phenotypes were localized to six different regions within the nadR gene. That mutations affecting repression and transport all mapped w...

journal_title：FEMS microbiology letters

authors： Foster JW,Penfound T

更新日期：1993-09-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter sulfidophilus is able to grow chemotrophically and phototrophically at a broad range of light intensities. In contrast to other facultative phototrophs, R. sulfidophilus synthesizes reaction center and light-harvesting (LH) complexes, B870 (LHI) and B800-850 (LHII) even under f...

journal_title：FEMS microbiology letters

authors： Hagemann GE,Gad'on N,Garcia A,Drews G,Tadros MH

更新日期：1995-02-01 00:00:00

abstract：:The lipid modification and processing of truncated mutants of the colicin A lysis protein were observed after overproduction by Escherichia coli bacteria, but at a rate far slower than that of the wild-type. The unmodified precursor form of the mutants was stable over hour(s). The truncated mutants provoked lethality,...

journal_title：FEMS microbiology letters

authors： Cavard D

更新日期：1994-04-01 00:00:00

abstract：:Bacterial swarming motility is a flagella-dependent translocation on the surface environment. It has received extensive attention as a population behavior involving numerous genes. Here, we report that Citrobacter freundii, an opportunistic pathogen, exhibits swarming movement on a solid medium surface with appropriat...

journal_title：FEMS microbiology letters

authors： Cong Y,Wang J,Chen Z,Xiong K,Xu Q,Hu F

更新日期：2011-04-01 00:00:00

abstract：:Because probiotic effects are strain dependent, genomic explanations of these differences will contribute to understanding their mechanisms of action. The genomic sequence of the Bifidobacterium longum probiotic strain NCC2705 was determined, but little is known about the genetic diversity between strains of this spec...

journal_title：FEMS microbiology letters

authors： Delcenserie V,Lessard MH,LaPointe G,Roy D

更新日期：2008-03-01 00:00:00

abstract：:Stability of the membrane-bound and purified H+-translocating NADH:ubiquinone oxidoreductase, Complex I, was studied. The loss of the enzyme activity is strongly increased by alkaline pH and dilution of the sample. Complex I inactivation is prevented specifically by a low concentration of Ca2+ and/or an intracellular ...

journal_title：FEMS microbiology letters

authors： Belevich G,Belevich N,Knuuti J,Verkhovskaya M

更新日期：2018-06-01 00:00:00

abstract：:Serratia entomophila and Serratia proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. The translated products of the sep genes are members of the toxin complex (Tc) family of insecticid...

journal_title：FEMS microbiology letters

authors： Hurst MR,Jones SM,Tan B,Jackson TA

更新日期：2007-10-01 00:00:00

abstract：:The genes encoding the desulforubidin type of dissimilatory sulfite reductase (Dsr) from the sulfate-reducing bacteria Desulfobacter vibrioformis and Desulfobulbus rhabdoformis were cloned and sequenced. Similar to the genes for dissimilatory sulfite reductase from the genera Archaeoglobus, Desulfovibrio and Desulfoto...

journal_title：FEMS microbiology letters

authors： Larsen,Lien T,Birkeland NK

更新日期：2000-05-01 00:00:00

abstract：:The effect of synthetic sex pheromone on pheromone-inducible conjugation between the isogenic Enterococcus faecalis strains OG1RF and OG1SS was investigated in (i) Todd-Hewitt broth medium and (ii) intestinal mucus isolated from germ-free rats. In broth, the presence of synthetic pheromone cCF10 had no detectable effe...

journal_title：FEMS microbiology letters

authors： Licht TR,Hammerum AM,Jensen LB,Jacobsen BL

更新日期：2001-11-13 00:00:00

abstract：:Peptidoglycan hydrolases are an effective new source of antimicrobials. A chimeric fusion protein of the Ply187 endopeptidase domain and LysK SH3b cell wall-binding domain is a potent agent against Staphylococcus aureus in four functional assays. ...

journal_title：FEMS microbiology letters

authors： Mao J,Schmelcher M,Harty WJ,Foster-Frey J,Donovan DM

更新日期：2013-05-01 00:00:00

abstract：:Sera, from 17 patients with diarrhoea or haemolytic uraemic syndrome, and six healthy adults, were tested for neutralisation of Vero cytotoxins (VT). For all 17 patients there was evidence of infection with Escherichia coli O157. Sera from two controls but from none of the patients neutralised VT1, although two patien...

journal_title：FEMS microbiology letters

authors： Scotland SM,Said B,Thomas A,Rowe B

更新日期：1994-01-15 00:00:00

abstract：:We report the construction of two broad host range promoter-probe plasmid vectors for rapid analysis of promoters in Gram-negative bacteria. The new vectors, pME4507 and pME4510, carry carbenicillin and gentamycin resistance genes, respectively, and are small sized (4 kb) with a flexible multiple cloning site to facil...

journal_title：FEMS microbiology letters

authors： Rist M,Kertesz MA

更新日期：1998-12-01 00:00:00

abstract：:ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity. However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications. Moreover, the cost involved in the preparation of Zinc Oxide-Nano-...

journal_title：FEMS microbiology letters

authors： Visnuvinayagam S,Murthy LN,Parvathy U,Jeyakumari A,Sivaraman GK,Karthikeyan D

更新日期：2020-12-15 00:00:00

abstract：:The gene (open reading frame (ORF) Tm1469, glk) encoding ATP-dependent ROK (repressors, ORFs, sugar kinases) glucokinase (ATP-GLK, EC 2.7.1.2) of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparen...

journal_title：FEMS microbiology letters

authors： Hansen T,Schönheit P

更新日期：2003-09-26 00:00:00

abstract：:We have isolated the abc1 gene from the fission yeast Schizosaccharomyces pombe. Sequence analysis suggests that the Abc1 protein is a member of the ABC superfamily of transporters and is composed of two structurally homologous halves, each consisting of a hydrophobic region of six transmembrane domains and a hydrophi...

journal_title：FEMS microbiology letters

authors： Christensen PU,Davis K,Nielsen O,Davey J

更新日期：1997-02-01 00:00:00