Abstract:
:ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity. However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications. Moreover, the cost involved in the preparation of Zinc Oxide-Nano-Particle (ZnO-NP) is a major limitation for commercial food applications. Hence a suitable alternative for ZnO-NP is highly needed for food application. Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH. Based on the study, it was observed that in the individual form of ZnO-NP possessed significantly higher antimicrobial activity than ZnO-BP. The composite form of ZnO-NP-CH and ZnO-BP-CH possessed higher antimicrobial activity than chitosan. However, no significant difference was observed between the composite forms. Hence, ZnO-BP-CH could be recommended as a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Visnuvinayagam S,Murthy LN,Parvathy U,Jeyakumari A,Sivaraman GK,Karthikeyan Ddoi
10.1093/femsle/fnaa210subject
Has Abstractpub_date
2020-12-15 00:00:00eissn
0378-1097issn
1574-6968pii
6035237pub_type
杂志文章abstract::To determine the existence of an acid stress response in Helicobacter pylori the global changes in the proteins synthesized by the bacterium when subjected to an acid stress were studied. H. pylori ATCC43504 previously adapted to pH 7 did not show an acid stress response as detected by the two-dimensional electrophore...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2002.tb11287.x
更新日期:2002-07-16 00:00:00
abstract::We used error-prone PCR to generate mutations in a subtilisin protease-encoding gene, and screened for recombinants that expressed temperature-sensitive (TS) variants. From the dozens of mutations that we detected in the recombinant genes we found that those mutations that affected aspartate-75 had the most profound e...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnaa162
更新日期:2020-10-16 00:00:00
abstract::Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxi...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2000.tb09222.x
更新日期:2000-08-15 00:00:00
abstract::Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiologica...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2007.00805.x
更新日期:2007-08-01 00:00:00
abstract::A DNA segment located immediately upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7 strain HA1 was cloned and sequenced. This segment contained an open reading frame encoding a predicted protein of 156 amino acids. A database search identified similar open reading frames upstream of the eaeA gene...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1995.tb07856.x
更新日期:1995-11-01 00:00:00
abstract::Streptococcus intermedius is well known to produce severe infections in various areas of the body. In this study, we evaluated the ability of S. intermedius to utilise human transferrin as a source of iron and investigated the mechanism by which iron can be obtained from this plasma protein. Adding either ferrous sulf...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1998.tb13193.x
更新日期:1998-09-01 00:00:00
abstract::When Escherichia coli STII was applied to the serosa of the ileum at a concentration of 40 micrograms/ml, an acceleration of the spontaneous motility and a weak contraction were induced 2-3 min later. The induction was not affected by the addition of atropine (10(-6) M), but was abolished by the addition of papaverine...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(92)90655-8
更新日期:1992-01-15 00:00:00
abstract::Salmonella Typhimurium harbors two Salmonella pathogenicity islands (SPIs), each encoding a type three secretion system for virulence proteins. Although there is increasing evidence of postinvasion roles for SPI-1, it has been generally accepted that SPI-1 genes are downregulated following the invasion process. Here, ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2011.02217.x
更新日期:2011-04-01 00:00:00
abstract::The transcriptional repressor Rex has been implicated in the regulation of energy metabolism and fermentative growth in response to redox potential. Streptococcus mutans, the primary causative agent of human dental caries, possesses a gene that encodes a protein with high similarity to members of the Rex family of pro...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2011.02293.x
更新日期:2011-07-01 00:00:00
abstract::Trinitrotoluene (TNT) is an explosive chemical generally used for military, civil and industrial purposes. Therefore, TNT residue can be found in soil and ground water as an environmental pollutant. The environmental control of TNT pollution has become a critical issue because of its potential toxicity and carcinogeni...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnx256
更新日期:2018-01-01 00:00:00
abstract::The anr gene of Pseudomonas aeruginosa encodes a transcriptional regulator of anaerobic gene expression, homologous to the Fnr protein of Escherichia coli. We report here that Anr has a role in regulating the activity of the aerobic respiratory chain of P. aeruginosa. Strains with internal deletions in their anr gene ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1997.tb12732.x
更新日期:1997-11-15 00:00:00
abstract::The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracel...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2001.tb10833.x
更新日期:2001-09-25 00:00:00
abstract::The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria to export virulence and colonization factors to their cell surface or into their surroundings. They are expressed as monomeric proteins that pass the inner and outer membrane in two consecutive steps facilitated by the Sec translo...
journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
doi:10.1093/femsle/fny165
更新日期:2018-09-01 00:00:00
abstract::Hydrogenotrophic methanogens live in a synthrophic relationship with the human gut microbiota as the terminal part of the anaerobic food chain. Methanobrevibacter smithii of the Methanobacteriales is the prevailing archaeal species. Recently, methylotrophic archaea of the novel order Methanomassiliicoccales were isola...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnv092
更新日期:2015-07-01 00:00:00
abstract::Tritrichomonas foetus is a venereal pathogen of cattle, which causes infertility, early embryonic death or abortion. In order to evaluate the potential trichomonicidal activity of benzimidazoles, the effects of thiabendazole, mebendazole and albendazole were analyzed on the multiplication, general morphology and ultra...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2007.00897.x
更新日期:2007-10-01 00:00:00
abstract::Improving the ability of plants and plant-associated organisms to fix and assimilate atmospheric nitrogen has inspired plant biotechnologists for decades, not only to alleviate negative effects on nature from increased use and availability of reactive nitrogen, but also because of apparent economic benefits and opport...
journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
doi:10.1093/femsle/fnx274
更新日期:2018-02-01 00:00:00
abstract::The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Es...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(92)90147-g
更新日期:1992-11-01 00:00:00
abstract::The synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB+ strain. Immunoblotting expe...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(89)90362-5
更新日期:1989-11-01 00:00:00
abstract::The physiological role of mitochondrial aldehyde dehydrogenase (ALD5) was investigated by analysis of the ald5 mutant (AKD321) in Saccharomyces cerevisiae. K(+)-activated ALDH activity of the ald5 mutant was about 80% of the wild-type in the mitochondrial fraction, while the respiratory activity of the ald5 mutant was...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb08856.x
更新日期:1999-12-15 00:00:00
abstract::Serine hydroxymethyltransferase (SHMT) is a key enzyme in cellular one-carbon pathway and has been studied in many living organisms from bacteria to higher plants and mammals. However, biochemical and molecular characterization of SHMT from photoautotrophic microorganisms remains a challenge. Here, we isolated the SHM...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2012.02597.x
更新日期:2012-08-01 00:00:00
abstract::We report the enhanced bactericidal activity of ofloxacin in drug-containing Eudragit E100(®) dispersions (EuCl-OFX) against Pseudomonas aeruginosa and the effect of the cationic polymer on bacterial membrane. Organisms treated with EuCl-OFX showed changes in cell morphology, altered outer membrane (OM) and cytoplasm...
journal_title:FEMS microbiology letters
pub_type: 信件
doi:10.1111/j.1574-6968.2012.02626.x
更新日期:2012-09-01 00:00:00
abstract::The formation of landomycin A or one of its derivatives (5,6-anhydrolandomycin A) in a heterologous strain has never been achieved. It has now been made possible by the coexpression of a cosmid containing all biosynthetic genes necessary to produce landomycin A together with a pathway-specific regulatory gene. As host...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00861-9
更新日期:2004-01-15 00:00:00
abstract::Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1998.tb13896.x
更新日期:1998-09-15 00:00:00
abstract::Nucleoside diphosphate kinase (NDK) is a housekeeping enzyme localized in cellular organelles and distributed in various organs in prokaryotes and eukaryotes. In Neurospora crassa, NDK-1 is suggested to control catalases in response to heat, oxidative stress and light. In this study, we identified the presence of NDK-...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2006.00329.x
更新日期:2006-08-01 00:00:00
abstract::Two thermostable phytases were identified from Thai isolates of Aspergillus japonicus BCC18313 (TR86) and Aspergillus niger BCC18081 (TR170). Both genes of 1404 bp length, coding for putative phytases of 468 amino acid residues, were cloned and transferred into Pichia pastoris. The recombinant phytases, r-PhyA86 and r...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2008.01399.x
更新日期:2009-01-01 00:00:00
abstract::Chagas disease, caused by Trypanosoma cruzi, affects six to seven million people worldwide. Treatment is based on benznidazole, producing several side effects and debatable efficacy, highlighting the need for new alternative drugs. We investigated the activity of four C-4 functionalized azlactone derivatives (EPA-27, ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnx020
更新日期:2017-02-01 00:00:00
abstract::We have observed significant cell lysis upon temperature up-shift of recombinant Escherichia coli cultures harboring CI857-repressed lambda-based expression vectors. This event, that becomes evident about 30-40 min after the heat shock, takes place when using the lambda promoter system in Ind- lysogenic strains, but n...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb13750.x
更新日期:1999-08-15 00:00:00
abstract::The study compared images of mature Streptococcus mutans biofilms captured at increasing magnification to determine which microscopy method is most acceptable for imaging the biofilm topography and the extracellular polymeric substance (EPS). In vitro S. mutans biofilms were imaged using (1) scanning electron microsco...
journal_title:FEMS microbiology letters
pub_type: 信件
doi:10.1111/1574-6968.12334
更新日期:2014-01-01 00:00:00
abstract::A group of bacterial strains formerly known as CDC group M-5 are opportunistic pathogens to humans. In 1993, a name, Neisseria weaveri, was proposed by two independent studies to harbor CDC group M-5 strains, namely N. weaveri Holmes et al. 1993 and N. weaveri Andersen et al. 1993, with two different 'type' strains. H...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2011.02485.x
更新日期:2012-03-01 00:00:00
abstract::The genes mphA and mphB encode macrolide 2'-phosphotransferases I and II, respectively, and they confer resistance to macrolide antibiotics in Escherichia coli. To study the expression of these genes in Gram-positive bacteria, we constructed recombinant plasmids that consisted of an mph gene and the pUB110 vector in B...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1998.tb12880.x
更新日期:1998-02-15 00:00:00