Chimeric Ply187 endolysin kills Staphylococcus aureus more effectively than the parental enzyme.

abstract：:Chromohalobacter marismortui VH1 was screened for its ability to utilise organosulfonate compounds at a range of NaCl concentrations. Only aminomethane sulfonate, of seven sulfonates tested, was utilised. Length of lag phase during growth on aminomethane sulfonate, as either nitrogen and/or sulfur source, increased wi...

journal_title：FEMS microbiology letters

authors： Ternan NG,McMullan G

更新日期：2002-01-22 00:00:00

abstract：:Cell-free protein synthesis has emerged in recent years as a powerful tool that can potentially transform the production of recombinant proteins. Cell-free protein synthesis harnesses the synthetic power of living cells while eliminating many of the constraints of traditional cell-based gene expression methods. Due to...

journal_title：FEMS microbiology letters

authors： Lee KH,Kim DM

更新日期：2018-09-01 00:00:00

abstract：:Strains of the filamentous gliding bacterium Vitreoscilla, LB13 and C1, are shown to be highly sensitive to UV-A (320-400 nm), with an LD50 of less than 20 kJ m(-2). Vitreoscilla LB13 can be protected from UV-A by including superoxide dismutase and catalase, separately or in combination, during the exposure, indicatin...

journal_title：FEMS microbiology letters

authors： Biggs LF,Moody AJ

更新日期：2003-07-15 00:00:00

abstract：:This study reports the production of astaxanthin in the photosynthetic bacterium Rhodovulum sulfidophilum, which has adequate precursor pools and storage capabilities for heterologous carotenoid production. Chemical mutagenesis was carried out using ethylmethane sulfonate to produce mutants with a modified carotenoid ...

journal_title：FEMS microbiology letters

authors： Mukoyama D,Takeyama H,Kondo Y,Matsunaga T

更新日期：2006-12-01 00:00:00

abstract：:In this study we have investigated the ability of nonencapsulated, nontypeable Haemophilus influenzae, NT477 to survive in the J774 mouse macrophage-like cell line. Viable, intracellular nontypeable H. influenzae could still be recovered from macrophages 72 h after phagocytosis. In contrast, H. influenzae strain Rd, a...

journal_title：FEMS microbiology letters

authors： Craig JE,Cliffe A,Garnett K,High NJ

更新日期：2001-09-11 00:00:00

abstract：:The assessment of scientific research is essentially based on several metric parameters, the so-termed Impact Factor perhaps being the predominant one. Despite well-founded criticisms and the wide opposition of reputed scientists, this procedure has become a tool of scientific policy, and is applied in editorial proce...

journal_title：FEMS microbiology letters

authors： Argüelles JC,Argüelles-Prieto R

更新日期：2019-06-01 00:00:00

abstract：:The ADP-ribosylating enzymes are encoded in many pathogenic bacteria in order to affect essential functions of the host. In this study, we show that Neisseria gonorrhoeae possess a locus that corresponds to the ADP-ribosyltransferase NarE, a previously characterized enzyme in N. meningitidis The 291 bp coding sequence...

journal_title：FEMS microbiology letters

authors： Rodas PI,Álamos-Musre AS,Álvarez FP,Escobar A,Tapia CV,Osorio E,Otero C,Calderón IL,Fuentes JA,Gil F,Paredes-Sabja D,Christodoulides M

更新日期：2016-09-01 00:00:00

abstract：:Tritrichomonas foetus is a venereal pathogen of cattle, which causes infertility, early embryonic death or abortion. In order to evaluate the potential trichomonicidal activity of benzimidazoles, the effects of thiabendazole, mebendazole and albendazole were analyzed on the multiplication, general morphology and ultra...

journal_title：FEMS microbiology letters

authors： Carvalho KP,Gadelha AP

更新日期：2007-10-01 00:00:00

abstract：:We recently identified the phaG(Pp) gene encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase in Pseudomonas putida, which directly links the fatty acid de novo biosynthesis and polyhydroxyalkanoate (PHA) biosynthesis. An open reading frame (ORF) of which the deduced amino acid sequence shared about 57% identity with P...

journal_title：FEMS microbiology letters

authors： Hoffmann N,Steinbüchel A,Rehm BH

更新日期：2000-03-15 00:00:00

abstract：:Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

journal_title：FEMS microbiology letters

authors： Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

更新日期：1999-04-01 00:00:00

abstract：:A hybridized DNA fragment was cloned as a 7.6-kb fragment from Streptomyces lavendulae KCCS0263 using a 1.9-kb SacI-XbaI DNA fragment from S. cellulosae as a probe. The latter fragment encoded a beta-lactamase which can bind blue dextran. The hybridized region was reduced to a 2.8-kb KpnI-BclI fragment and the nucleot...

journal_title：FEMS microbiology letters

authors： Sendouda A,Urabe H,Ogawara H

更新日期：1993-09-15 00:00:00

abstract：:Two clones, obtained by polymerase chain reaction from macronuclear DNA of the unicellular ciliated protist Blepharisma japonicum, were isolated and sequenced. They correspond to fragments of two different putative H4 histone genes. The existence of multiple H4 histone genes was also suggested by Southern blot hybridi...

journal_title：FEMS microbiology letters

authors： Salvini M,Bini E,Santucci A,Batistoni R

更新日期：1997-04-01 00:00:00

abstract：:Emergence of races in Fusarium oxysporum f. sp. lycopersici (Fol) is caused by loss or mutation of at least one avirulence (AVR) gene. The product of AVR1 is a small protein (Avr1) secreted by Fol in tomato xylem sap during infection. This protein triggers Fol race 1 specific resistance (I) in tomato, indicating that ...

journal_title：FEMS microbiology letters

authors： Kashiwa T,Suzuki T,Sato A,Akai K,Teraoka T,Komatsu K,Arie T

更新日期：2016-07-01 00:00:00

abstract：:Bacteriophages co-exist and co-evolve with their hosts in natural environments. Virulent phages lyse infected cells through lytic cycles, whereas temperate phages often remain dormant and can undergo lysogenic or lytic cycles. In their lysogenic state, prophages are actually part of the host genome and replicate passi...

journal_title：FEMS microbiology letters

authors： Menouni R,Hutinet G,Petit MA,Ansaldi M

更新日期：2015-01-01 00:00:00

abstract：:Legionella pneumophila, an intracellular parasite of macrophages and protozoa, requires iron for extra- and intracellular growth. In a new screen of a mutant library of L. pneumophila for strains defective for growth on agar media lacking supplemental iron, seven mutants were obtained. All of the mutants had a disrupt...

journal_title：FEMS microbiology letters

authors： Naylor J,Cianciotto NP

更新日期：2004-12-15 00:00:00

abstract：:In this study, the biodiversity of 154 strains of lactic acid bacteria, including 112 dairy product isolates presumptively identified as obligately heterofermentative lactobacilli (OHL) by classical microbiological tests, as well as 23 OHL-type strains, was investigated by PCR-based methods and gene sequencing. Using ...

journal_title：FEMS microbiology letters

authors： Coton M,Berthier F,Coton E

更新日期：2008-07-01 00:00:00

abstract：:The electron transfer pathway to Fe(III) reduction in Geobacter sulfurreducens has been hypothesized to consist of a series of c-type cytochromes. Previous genetic studies suggested that the inner membrane-associated, c-type cytochrome, MacA, was a component of the electron transfer chain leading to Fe(III) reduction ...

journal_title：FEMS microbiology letters

authors： Kim BC,Lovley DR

更新日期：2008-09-01 00:00:00

abstract：:The Gram-negative anaerobe Dichelobacter nodosus is the causative agent of footrot in sheep. Different strains of D. nodosus cause disease of differing severities, ranging from benign to virulent. Virulent strains have greater twitching motility and secrete proteases that are more thermostable than those secreted by b...

journal_title：FEMS microbiology letters

authors： Palanisamy SK,Fletcher C,Tanjung L,Katz ME,Cheetham BF

更新日期：2010-01-01 00:00:00

abstract：:Salmonella enteritidis isolated from poultry infections generated a convoluted colonial morphology after 48 h growth on colonisation factor antigen (CFA) agar at 25 degrees C. A mutant S. enteritidis defective for the elaboration of the SEF17 fimbrial antigen, in which the agf gene cluster was inactivated by insertion...

journal_title：FEMS microbiology letters

authors： Allen-Vercoe E,Dibb-Fuller M,Thorns CJ,Woodward MJ

更新日期：1997-08-01 00:00:00

abstract：:Cyanobacterial natural products offer new possibilities for drugs and lead compounds but many factors can inhibit the production of sufficient yields for pharmaceutical processes. While Escherichia coli and Streptomyces sp. have been used as heterologous expression hosts to produce cyanobacterial natural products, the...

journal_title：FEMS microbiology letters

authors： Wells KN,Videau P,Nelson D,Eiting JE,Philmus B

更新日期：2018-08-01 00:00:00

abstract：:Pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. Computer analysis of the P. ananatis SC17(0) sequenced genome revealed an ORF encoding a homologue (named gcd) of the mGDH (EC 1.1.99.17) apoenzyme from Escherichia coli and a putative pyrroloquinoline quinone (PQQ) biosynthetic o...

journal_title：FEMS microbiology letters

authors： Andreeva IG,Golubeva LI,Kuvaeva TM,Gak ER,Katashkina JI,Mashko SV

更新日期：2011-05-01 00:00:00

abstract：:The technique based on fluorescein-linked lectins used to determine the cell wall structure of anaerobic rumen fungi belonging to genera: Neocallimastix, Piromonas and Sphaeromonas, appears to be an interesting tool for distinguishing between strains. Furthermore this technique shows differences of cell wall compositi...

journal_title：FEMS microbiology letters

authors： Guillot J,Breton A,Damez M,Dusser M,Gaillard-Martinie B,Millet L

更新日期：1990-01-15 00:00:00

abstract：:An obligately anaerobic spirochete designated strain SEBR 4228T (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration) growth range 1.0-10%) at 37 degrees C (growth temperature range 20-40 degrees C...

journal_title：FEMS microbiology letters

authors： Magot M,Fardeau ML,Arnauld O,Lanau C,Ollivier B,Thomas P,Patel BK

更新日期：1997-10-15 00:00:00

abstract：:Haemin (iron protoporphyrin IX) is an essential growth factor for the periodontal pathogen. Porphyromonas gingivalis. Iron protoporphyrin IX (IPP IX) binding to the avirulent P. gingivalis beige variant (W50/BE1) and the black-pigmenting parent wild-type strain W50 was quantified. W50/BE1 grown in a chemostat under ha...

journal_title：FEMS microbiology letters

authors： Smalley JW,Birss AJ,McKee AS,Marsh PD

更新日期：1996-07-15 00:00:00

abstract：:The aim of this study was to illustrate the relative pervasiveness of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) in community and food of animal origin and their relationship with other genetic determinants. Staphylococcus aureus isolates were su...

journal_title：FEMS microbiology letters

authors： Zehra A,Gulzar M,Singh R,Kaur S,Gill JPS

更新日期：2020-12-22 00:00:00

abstract：:The synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB+ strain. Immunoblotting expe...

journal_title：FEMS microbiology letters

authors： Sumantran VN,Tranguch AJ,Datta P

更新日期：1989-11-01 00:00:00

abstract：:A family of 2,3-di-O-acyl trehaloses (DAT), previously identified in Mycobacterium fortuitum, was studied by fast-atom bombardment mass spectrometry to establish the combinations of fatty acyl substituents and, hence, to delineate the molecular species there comprised. The mass spectra indicated the possible existence...

journal_title：FEMS microbiology letters

authors： Ariza MA,Valero-Guillén PL

更新日期：1994-06-15 00:00:00

abstract：:The gene (open reading frame (ORF) Tm1469, glk) encoding ATP-dependent ROK (repressors, ORFs, sugar kinases) glucokinase (ATP-GLK, EC 2.7.1.2) of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparen...

journal_title：FEMS microbiology letters

authors： Hansen T,Schönheit P

更新日期：2003-09-26 00:00:00

abstract：:The gene nfxB is one of the genes which affect the cell membrane permeability of quinolones in Pseudomonas aeruginosa PAO. Both wild-type nfxB and a mutant nfxB (nfx13E) were cloned and the DNA sequences were determined. The wild-type gene was dominant in PAO strains. The nfxB mutation was a point mutation (cytosine--...

journal_title：FEMS microbiology letters

authors： Okazaki T,Hirai K

更新日期：1992-10-01 00:00:00

abstract：:Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four gr...

journal_title：FEMS microbiology letters

authors： Casas C,Anderson EC,Ojo KK,Keith I,Whelan D,Rainnie D,Roberts MC

更新日期：2005-01-01 00:00:00