Abstract:
:In this study, the biodiversity of 154 strains of lactic acid bacteria, including 112 dairy product isolates presumptively identified as obligately heterofermentative lactobacilli (OHL) by classical microbiological tests, as well as 23 OHL-type strains, was investigated by PCR-based methods and gene sequencing. Using these techniques, 51% of the cheese isolates were actually identified as OHL. The non-OHL isolates were identified to the Leuconostoc, Lactobacillus, Weisella, Pediococcus or Streptococcus genera. Among the OHL cheese isolates, five species were directly identified including three of the most frequently isolated species -Lactobacillus fermentum, Lactobacillus parabuchneri and Lactobacillus brevis- and two rarely isolated species - Lactobacillus diolivorans and Lactobacillus reuteri. A sixth group made up of two dairy isolates was also identified and according to 16S rRNA gene and intergenic spacer region (ISR) sequencing data corresponded to Lactobacillus sp. and may constitute a new species. Species-specific primers were designed for the rapid and reliable detection of the three most frequently isolated species by species-specific duplex PCR.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Coton M,Berthier F,Coton Edoi
10.1111/j.1574-6968.2008.01206.xsubject
Has Abstractpub_date
2008-07-01 00:00:00pages
150-7issue
2eissn
0378-1097issn
1574-6968pii
FML1206journal_volume
284pub_type
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journal_title:FEMS microbiology letters
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