Abstract:
:A succinate semialdehyde dehydrogenase gene (gabD) was identified to be disrupted in a transposon-induced mutant of Ralstonia eutropha exhibiting the phenotype 4-hydroxybutyric acid-leaky. The native gabD gene was cloned by colony hybridization using a homologous gabD-specific DNA probe. DNA sequencing revealed an 1452-bp open reading frame, and the deduced amino acid sequence showed strong similarities to NADP(+)-dependent succinate semialdehyde dehydrogenases from Escherichia coli, Rhizobium sp., Homo sapiens and Rattus norvegicus. The gabD gene was heterologously expressed in a recombinant E. coli strain harboring plasmid pSK::EE6.8. Similar to the molecular organization of the gab cluster in E. coli, additional genes encoding enzymes for the degradation of gamma-aminobutyrate are closely related to gabD in R. eutropha. Enzymatic studies indicated the existence of a second NAD(+)-dependent succinate semialdehyde dehydrogenase in R. eutropha.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Lütke-Eversloh T,Steinbüchel Adoi
10.1111/j.1574-6968.1999.tb08827.xkeywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
63-71issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00515-7journal_volume
181pub_type
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