The ability of Vibrio vulnificus to use a synthetic hydrophilic heme compound, Fe-TPPS, as a single iron source.

Abstract:

:Vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. This process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin IX). In the present study, we tested whether this pathogen also uses a synthetic heme compound, Fe-alpha,beta,gamma,delta-tetraphenylporphine tetrasulfonic acid (Fe-TPPS), as an iron source. When inoculated into a medium containing Fe-TPPS, V. vulnificus L-180 multiplication was seen to be dependent on the concentration of the synthetic heme compound; a mutant lacking the ability to utilize protoheme did not multiply. Cells of the strain grown under the iron-restricted condition showed time-dependent uptake of Fe-TPPS. The ability to use either protoheme or Fe-TPPS was significantly reduced by the addition of an excess amount of free TPPS or Cu-TPPS. The data suggest that, V. vulnificus may assimilate Fe-TPPS, at least partially, through the same system as that for protoheme.

journal_name

FEMS Microbiol Lett

authors

Miyoshi S,Kamei T,Inami Y,Ota Y,Yamamoto S,Tomochika K,Shinoda S

doi

10.1111/j.1574-6968.1999.tb13452.x

keywords:

subject

Has Abstract

pub_date

1999-03-01 00:00:00

pages

73-7

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109799000166

journal_volume

172

pub_type

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