General and kinetic properties of endoglucanase from Aspergillus niger.

abstract：:A succinate semialdehyde dehydrogenase gene (gabD) was identified to be disrupted in a transposon-induced mutant of Ralstonia eutropha exhibiting the phenotype 4-hydroxybutyric acid-leaky. The native gabD gene was cloned by colony hybridization using a homologous gabD-specific DNA probe. DNA sequencing revealed an 145...

journal_title：FEMS microbiology letters

authors： Lütke-Eversloh T,Steinbüchel A

更新日期：1999-12-01 00:00:00

abstract：:Three different whole cell biosensor constructs were made by fusing the mercury inducible promoter, P(mer), and its regulatory gene, merR, from transposon Tn21 with reporter genes luxCDABE, lacZYA, or gfp. In Escherichia coli these biosensor constructs responded to low levels of mercury by producing light, beta-galact...

journal_title：FEMS microbiology letters

authors： Hansen LH,Sørensen SJ

更新日期：2000-12-01 00:00:00

abstract：:In the present study, strains of 17 reference Acinetobacter genospecies were investigated by using the polymerase chain reaction (PCR). We used primers to amplify spacer regions between the 16S and 23S genes in the prokaryotic rRNA genetic loci. When the spacer amplification products were resolved by electrophoresis, ...

journal_title：FEMS microbiology letters

authors： Nowak A,Burkiewicz A,Kur J

更新日期：1995-02-15 00:00:00

abstract：:The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which were characterised by methylation analysis and 1D and 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) and (1-->6) linkages, a structure which is mo...

journal_title：FEMS microbiology letters

authors： Reis RA,Tischer CA,Gorin PA,Iacomini M

更新日期：2002-04-23 00:00:00

abstract：:Many antibiotic-producing actinomycetes possess at least one ABC (ATP-binding cassette) transporter which forms part of the antibiotic biosynthetic pathway and in most cases confers resistance to the drug in an heterologous host. Three types of antibiotic ABC transporters have been so far described in producer organis...

journal_title：FEMS microbiology letters

authors： Méndez C,Salas JA

更新日期：1998-01-01 00:00:00

abstract：:In this study, we analysed the diversity and composition of double-stranded DNA viral and bacterial communities within the sample of surface coastal water of Southern Baikal through metagenomics and deep sequencing of the 16S ribosomal RNA gene, respectively. The 16S rRNA gene analysis has revealed 14 phyla and domina...

journal_title：FEMS microbiology letters

authors： Butina TV,Bukin YS,Krasnopeev AS,Belykh OI,Tupikin AE,Kabilov MR,Sakirko МV,Belikov SI

更新日期：2019-05-01 00:00:00

abstract：:Helicobacter pylori possesses a gene (HP0326/JHP309) homologous to neuA of other bacteria, encoding a cytidyl monophosphate-N-acetylneuraminic acid synthetase-homologous enzyme in its N-terminal portion. We analysed the function of this gene, which is controlled by a flagellar class 2 sigma(54) promoter, in flagellar ...

journal_title：FEMS microbiology letters

authors： Josenhans C,Vossebein L,Friedrich S,Suerbaum S

更新日期：2002-05-07 00:00:00

abstract：:Cowpea (Vigna unguiculata) and mung bean (Vigna radiata) are important legume crops yet their rhizobia have not been well characterized. In the present study, 62 rhizobial strains isolated from the root nodules of these plants grown in the subtropical region of China were analyzed via a polyphasic approach. The result...

journal_title：FEMS microbiology letters

authors： Zhang YF,Wang ET,Tian CF,Wang FQ,Han LL,Chen WF,Chen WX

更新日期：2008-08-01 00:00:00

abstract：:The aim of this study was to screen two cheese starter cultures and cheese-borne microbial communities with the potential to produce biogenic amines in cheese during ripening. Bacteria of the genera Enterococcus and Lactobacillus and coliform bacteria were isolated from Dutch-type semi-hard cheese at the beginning of ...

journal_title：FEMS microbiology letters

authors： Burdychova R,Komprda T

更新日期：2007-11-01 00:00:00

abstract：:The steady-state levels of the aminopeptidase Y (APY1) transcript and the level of assayable aminopeptidase Y activity were measured under a variety of nutritional conditions. Specific APY1 mRNA is less abundant in cells grown in minimal medium than in cells grown in rich medium, while active enzyme levels follow just...

journal_title：FEMS microbiology letters

authors： Herrera-Camacho I,Suárez-Rendueles P

更新日期：1996-06-01 00:00:00

abstract：:In the dental caries pathogen Streptococcus mutans, an MarR-like transcriptional regulator (RcrR), two ABC efflux pumps (RcrPQ) and two effector peptides encoded in the rcrRPQ operon provide molecular connections between stress tolerance, (p)ppGpp metabolism and genetic competence. Here, we examined the role of RcrRPQ...

journal_title：FEMS microbiology letters

authors： Shields RC,Burne RA

更新日期：2015-08-01 00:00:00

abstract：:An exo-polygalacturonase (EC 3.2.1.15) was purified to apparent homogeneity from cultures of Fusarium oxysporum f.sp. lycopersici on synthetic medium supplemented with citrus pectin, using preparative isoelectric focusing. The enzyme, denominated PG2, had an apparent M(r) of 74000 Da upon SDS-PAGE. The pI of the main ...

journal_title：FEMS microbiology letters

authors： Di Pietro A,Roncero MI

更新日期：1996-12-01 00:00:00

abstract：:A bacterial isolate, tentatively identified as Pseudomonas sp. strain TR3, was found to utilize the diaryl ester phenylbenzoate as sole source of carbon and energy. This strain has the ability to productively degrade phenylbenzoate and some substituted derivatives by a catabolic sequence which was characterized bioche...

journal_title：FEMS microbiology letters

authors： Reich T,Schmidt S,Fortnagel P

更新日期：1999-07-15 00:00:00

abstract：:NadR is the repressor protein that controls the expression of genes for NAD synthesis. It is also believed to be involved in nucleotide transport. Point mutations conferring different phenotypes were localized to six different regions within the nadR gene. That mutations affecting repression and transport all mapped w...

journal_title：FEMS microbiology letters

authors： Foster JW,Penfound T

更新日期：1993-09-01 00:00:00

abstract：:Alkyl ether-degrading Rhodococcus sp. strain DEE5151, isolated from activated sewage sludge, has an activity for the oxidation of a variety of alkyl ethers, aralkyl ethers and dibenzyl ether. The whole cell activity for diethyl ether oxidation was effectively inhibited by 2,3-dihydrofurane, ethyl vinyl ether and gluta...

journal_title：FEMS microbiology letters

authors： Kim YH,Engesser KH

更新日期：2005-02-15 00:00:00

abstract：:In this study, internal size variations in the VanA gene cluster Tn1546, encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn1546, revealed that these variations were due to the presence of the IS sequenc...

journal_title：FEMS microbiology letters

authors： Jensen LB

更新日期：1998-12-15 00:00:00

abstract：:In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb...

journal_title：FEMS microbiology letters

authors： Yang Y,Li C

更新日期：2009-01-01 00:00:00

abstract：:Piscirickettsia salmonis is a novel, aggressive, facultative Gram-negative bacterium that drastically affects salmon production at different latitudes, with particular impact in southern Chile. Initially, P. salmonis was described as a Rickettsia-like, obligate, intracellular Alphaproteobacteria, but it was reclassifi...

journal_title：FEMS microbiology letters

authors： Marshall SH,Henríquez V,Gómez FA,Cárdenas C

更新日期：2011-01-01 00:00:00

abstract：:We describe a new procedure allowing the generation and detection of immunogenic antigens from Helicobacter pylori via the hemolysin secretion apparatus of Escherichia coli. The gene (or gene fragment) encoding the H. pylori protein (or protein domain) is inserted in-frame into a residual portion of the hemolysin gene...

journal_title：FEMS microbiology letters

authors： Spreng S,Gentschev I,Goebel W,Mollenkopf H,Eck M,Müller-Hermelink HK,Schmausser B

更新日期：2000-05-15 00:00:00

abstract：:Two Pseudomonas aeruginosa genes encoding the enzymes 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase, both involved in the mevalonate-independent biosynthesis of isoprenoids, have been expressed as recombinant enzymes in Escherichia coli. The purified P. aeruginosa DXP reductoisomerase was inhi...

journal_title：FEMS microbiology letters

authors： Altincicek B,Hintz M,Sanderbrand S,Wiesner J,Beck E,Jomaa H

更新日期：2000-09-15 00:00:00

abstract：:Mycobacterium sp. PYR-1 was previously isolated in our laboratory and was shown to be able to mineralize high molecular mass polycyclic aromatic hydrocarbons (PAHs) [Heitkamp and Cerniglia, (1988) Appl. Environ. Microbiol. 54, 1612-1614]. In this research, the 16S rRNA gene (rDNA) of this strain was amplified by polym...

journal_title：FEMS microbiology letters

authors： Wang RF,Cao WW,Cerniglia CE

更新日期：1995-07-15 00:00:00

abstract：:Mapping of the plasmid-encoded RNA of the intracellular parasite, Chlamydia trachomatis revealed that the upstream control elements are different from those of other Gram-negative bacteria. A tetranucleotide, AYAA was found near the -10 position, in 5 out of 8 upstream sequences described so far. The plasmid also has ...

journal_title：FEMS microbiology letters

authors： Sriprakash KS,Pearce BJ

更新日期：1990-09-15 00:00:00

abstract：:We investigated the hemolytic activity of Escherichia coli strain EC901 carrying plasmid pBJN406 containing genes draA-E involved in expression of the mannose-resistant Dr hemagglutinin, and in its isogenic insertion mutants devised with Tn5, Tn3, and TnphoA. While E. coli BN406 displayed rapid hemolytic activity agai...

journal_title：FEMS microbiology letters

authors： Goluszko P,Nowicki S,Kaul AK,Pham T,Nowicki BJ

更新日期：1995-07-15 00:00:00

abstract：:Defects in the acd gene (which may be allelic to ubiH) result in the inactivation of the coenzyme A-linked acetaldehyde dehydrogenase activity of the multifunctional AdhE protein of Escherichia coli. This activity is restored by addition of ubiquinone-0 to cell extracts. However, the alcohol dehydrogenase activity of ...

journal_title：FEMS microbiology letters

authors： Gupta S,Mat-Jan F,Latifi M,Clark DP

更新日期：2000-01-01 00:00:00

abstract：:The gene encoding the alpha-subunit of methanol dehydrogenase (mxaF) and its flanking region was isolated from a methylotrophic bacterium, Hyphomicrobium methylovorum GM2. The deduced amino acid sequence of MxaF showed 80, 80, 74 and 66% identity with those of Methylobacterium extorquens AM1, M. organophilum XX, Parac...

journal_title：FEMS microbiology letters

authors： Tanaka Y,Yoshida T,Watanabe K,Izumi Y,Mitsunaga T

更新日期：1997-09-15 00:00:00

abstract：:Individual isolates of Escherichia coli synthesize one of more than 70 chemically distinct polysaccharides which form the capsule. In this article we review the genetics of capsule production in E. coli and highlight what this is beginning to reveal in terms of the genetic basis of the structural diversity of polysacc...

journal_title：FEMS microbiology letters

authors： Boulnois G,Drake R,Pearce R,Roberts I

更新日期：1992-12-15 00:00:00

abstract：:A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was pro...

journal_title：FEMS microbiology letters

authors： Wynter C,Patel BK,Bain P,de Jersey J,Hamilton S,Inkerman PA

更新日期：1996-07-01 00:00:00

abstract：:Using primers corresponding to conserved regions of the bacterial regulatory gene fur, a homologue of this gene from the genome of Rhizobium leguminosarum biovar viciae, the nitrogen-fixing symbiont of peas, was isolated and sequenced. The fur gene is normally expressed constitutively, independent of the presence of F...

journal_title：FEMS microbiology letters

authors： de Luca NG,Wexler M,Pereira MJ,Yeoman KH,Johnston AW

更新日期：1998-11-15 00:00:00

abstract：:Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. The synthesis of known virulence factors in this organism, such as extracellular enzymes and biofilm, is strictly regulated in response to environmental stimuli. Two-component signal transduction systems sense e...

journal_title：FEMS microbiology letters

authors： Tao J,He C

更新日期：2010-03-01 00:00:00

abstract：:This study reports on the antifungal activities of statins combined with an antifungal compound secreted by Penicillium chrysogenum, PAF. Several species belonging in the class Zygomycetes are considered to be agents of human or animal mycoses; other species have significance as postharvest plant pathogens. In the pre...

journal_title：FEMS microbiology letters

authors： Galgóczy L,Papp T,Lukács G,Leiter E,Pócsi I,Vágvölgyi C

更新日期：2007-05-01 00:00:00