Estimate of the diversity of viral and bacterial assemblage in the coastal water of Lake Baikal.

Abstract:

:In this study, we analysed the diversity and composition of double-stranded DNA viral and bacterial communities within the sample of surface coastal water of Southern Baikal through metagenomics and deep sequencing of the 16S ribosomal RNA gene, respectively. The 16S rRNA gene analysis has revealed 14 phyla and dominance of the 'Actinobacteria' (43.6%), 'Proteobacteria' (25.2%) and 'Bacteroidetes' (11.5%). The bacterial composition was similar to that obtained previously in Lake Baikal littoral zone. Out of 1 030 169 processed virome reads, 37.4% of sequences (385 421) were identified as viral; 15.1% were identified as nonviral and related to the domains Eukarya, Bacteria and Archaea; and 47.5% had no matches in the databases. The identified virotypes belonged to different families and were predicted to infect a wide range of organisms, from bacteria to mammals. Six families (Myoviridae, Poxviridae, Mimiviridae, Siphoviridae, Phycodnaviridae and Podoviridae) were dominant accounting for more than 90% of the identified sequences (48.3%, 17.4%, 8.3%, 6.8%, 5.8% and 4.1%, respectively). In contrast to other freshwater systems, high percentage of the Poxviridae and Mimiviridae was recorded in the water sample of Lake Baikal.

journal_name

FEMS Microbiol Lett

authors

Butina TV,Bukin YS,Krasnopeev AS,Belykh OI,Tupikin AE,Kabilov MR,Sakirko МV,Belikov SI

doi

10.1093/femsle/fnz094

subject

Has Abstract

pub_date

2019-05-01 00:00:00

issue

9

eissn

0378-1097

issn

1574-6968

pii

5484837

journal_volume

366

pub_type

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