Versatile biosensor vectors for detection and quantification of mercury.

abstract：:The phyCg gene encoding a new phytase from Citrobacter gillenii was optimized, synthesized, cloned and expressed in Pichia pastoris. Analysis of the amino acid sequence of the enzyme showed that it belongs to the histidine acid phosphatase family. The amino acid sequence of the PhyCg phytase has the highest homology (...

journal_title：FEMS microbiology letters

authors： Tkachenko AA,Kalinina AN,Borshchevskaya LN,Sineoky SP,Gordeeva TL

更新日期：2020-12-21 00:00:00

abstract：:We recently described adhesion to and invasion of bovine mammary gland cells by Staphylococcus aureus in vitro. Here, we show that the levels of adhesion and invasion are dependent on the bacterial growth phase and are controlled by the agr locus. Incubation of exponential growth phase cells of S. aureus with mammary ...

journal_title：FEMS microbiology letters

authors： Lammers A,Nuijten PJ,Smith HE

更新日期：1999-11-01 00:00:00

abstract：:The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning and sequencing of t...

journal_title：FEMS microbiology letters

authors： Kling JJ,Wright RL,Moncrief JS,Wilkins TD

更新日期：1997-01-15 00:00:00

abstract：:Subtilin is an antimicrobial peptide of the lantibiotic family that is produced by Gram-positive Bacillus subtilis, and its biosynthesis involves expression of presubtilin which consists of a leader segment and a mature segment. The leader segment is unlike a typical sec-type general secretion signal, and its ability ...

journal_title：FEMS microbiology letters

authors： Paul LK,Izaguirre G,Hansen JN

更新日期：1999-07-01 00:00:00

abstract：:Atypical enteropathogenic Escherichia coli (EPEC) comprise an important group of paediatric pathogens. Atypical EPEC have reservoirs in farm and domestic animals where they can be either commensal or pathogenic; serogroup O26 is dominant in humans and animals. Central to intestinal colonization by EPEC is the transloc...

journal_title：FEMS microbiology letters

authors： Ooka T,Vieira MA,Ogura Y,Beutin L,La Ragione R,van Diemen PM,Stevens MP,Aktan I,Cawthraw S,Best A,Hernandes RT,Krause G,Gomes TA,Hayashi T,Frankel G

更新日期：2007-06-01 00:00:00

abstract：:In this study, the biodiversity of 154 strains of lactic acid bacteria, including 112 dairy product isolates presumptively identified as obligately heterofermentative lactobacilli (OHL) by classical microbiological tests, as well as 23 OHL-type strains, was investigated by PCR-based methods and gene sequencing. Using ...

journal_title：FEMS microbiology letters

authors： Coton M,Berthier F,Coton E

更新日期：2008-07-01 00:00:00

abstract：:To tackle antimicrobial resistance (AMR) is of outmost importance for the general population to understand the severity and the relevance of different routes of transmission. Respondents of different age groups, educational and occupational backgrounds, area of living, diet and household composition participated in an...

journal_title：FEMS microbiology letters

authors： Alexa Oniciuc EA,Likotrafiti E,Garre A,Ruiz L,Prieto M,Alvarez-Ordóñez A

更新日期：2019-09-01 00:00:00

abstract：:The Mfa1 protein of Porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between P. gingivalis and Streptococcus gordonii. We utilized a promoter-lacZ reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. Promoter acti...

journal_title：FEMS microbiology letters

authors： Park Y,James CE,Yoshimura F,Lamont RJ

更新日期：2006-09-01 00:00:00

abstract：:In haloarchaea, a cluster of three genes is localized directly adjacent to the major replication origin, and, hence, the encoded proteins were annotated as 'origin-associated proteins' (Oap). However, prior to this study, no experimental data were available for these conserved hypothetical proteins. Bioinformatic anal...

journal_title：FEMS microbiology letters

authors： Wolters M,Borst A,Pfeiffer F,Soppa J

更新日期：2019-11-01 00:00:00

abstract：:Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization of B. anthracis isol...

journal_title：FEMS microbiology letters

authors： Ramisse V,Patra G,Garrigue H,Guesdon JL,Mock M

更新日期：1996-11-15 00:00:00

abstract：:It has been widely reported that pH mediates cadmium toxicity to bacteria. We used a tripartite approach to investigate mechanisms by which pH affects cadmium toxicity that included analyses of: (1) growth kinetics, (2) global gene expression, and (3) cadmium speciation. Cadmium extended the lag phase at pH 7, but not...

journal_title：FEMS microbiology letters

authors： Worden CR,Kovac WK,Dorn LA,Sandrin TR

更新日期：2009-04-01 00:00:00

abstract：:Because of an association of human neuroborreliosis with the development of an antibody response against an antigen in neural tissue that cross-reacts with an epitope on the flagellin protein of Borrelia burgdorferi, C3H transgenic mice were created that expressed the flagellin epitope (amino acids 213-224) as a fusio...

journal_title：FEMS microbiology letters

authors： Fikrig E,Barthold SW

更新日期：1997-03-15 00:00:00

abstract：:Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. The synthesis of known virulence factors in this organism, such as extracellular enzymes and biofilm, is strictly regulated in response to environmental stimuli. Two-component signal transduction systems sense e...

journal_title：FEMS microbiology letters

authors： Tao J,He C

更新日期：2010-03-01 00:00:00

abstract：:One hundred and ninety-eight clinical isolates of Mycobacterium kansasii collected between 2003 and 2004 in Japan were genotyped by PCR and restriction enzyme analysis (PRA) and 16S-23S internal transcribed spacer (ITS) sequencing. The results demonstrated that clinical isolates of M. kansasii in Japan are almost excl...

journal_title：FEMS microbiology letters

authors： Iwamoto T,Saito H

更新日期：2006-01-01 00:00:00

abstract：:Induction, energy gain, effect on growth, and interaction of nitrate and nitrite reduction of Bradyrhizobium sp. (Lupinus) USDA 3045 were characterized. Both nitrate and nitrite were reduced in air, although nitrite reduction was insensitive to ammonium inhibition. Anaerobic reduction of both ions was shown to be link...

journal_title：FEMS microbiology letters

authors： Polcyn W,Luciński R

更新日期：2003-09-26 00:00:00

abstract：:Nocardia asteroides complex organisms derived from human specimens between 1979 and 1992 were identified to the species level. Of 117 N. asteroides complex organisms, 34 (29%) were N. farcinica, 28 (24%) were N. nova, and 55 (47%) were N. asteroides sensu stricto. An analysis of the specimen sites from which the organ...

journal_title：FEMS microbiology letters

authors： Desmond EP,Flores M

更新日期：1993-07-01 00:00:00

abstract：:This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

journal_title：FEMS microbiology letters

authors： Nastos S,Rangachari PK

更新日期：2016-08-01 00:00:00

abstract：:Isolated membranes of the extreme haloalkaliphilic archaeon Natronococcus occultus were able to hydrolyze ATP via an ATPase, which required the presence of Mg(2+), high concentrations of NaCl, and a pH value of 9. The native molecular mass of the purified ATPase was 130 kDa and was composed of 74- and 61-kDa subunits....

journal_title：FEMS microbiology letters

authors： Eddy ML,Jablonski PE

更新日期：2000-08-15 00:00:00

abstract：:The accumulation of 2-keto-3-deoxy-6-phosphogluconate, the key intermediate of the Entner-Doudoroff pathway, has long been thought to inhibit growth of bacteria, but careful measurements of 2-keto-3-deoxy-6-phosphogluconate accumulation by growing cells and the correlation of intracellular 2-keto-3-deoxy-6-phosphogluc...

journal_title：FEMS microbiology letters

authors： Fuhrman LK,Wanken A,Nickerson KW,Conway T

更新日期：1998-02-15 00:00:00

abstract：:The effect of coumarin (1,2 benzopyrone) on glucose utilisation by the anaerobic rumen fungi Neocallimastix frontalis and N. patriciarum has been compared with the effect of p-coumaric acid. Both compounds largely inhibited glucose utilisation by N. patriciarum strain Cx when present in the medium at a concentration o...

journal_title：FEMS microbiology letters

authors： Cansunar E,Richardson AJ,Wallace G,Stewart CS

更新日期：1990-07-01 00:00:00

abstract：:Five independent collections, comprising a total of 34 clones encoding cellulases, hemicellulases and cell surface proteins of Clostridium thermocellum, were searched for overlapping or contiguous DNA fragments. The clones were hybridized to large genomic restriction fragments separated by pulse-field electrophoresis....

journal_title：FEMS microbiology letters

authors： Guglielmi G,Béguin P

更新日期：1998-04-01 00:00:00

abstract：:Phylogenetic relationships between Tuber pseudoexcavatum and other Tuber species were investigated by studying the sequences of four genes: 5.8S-ITS2, beta-tubulin, protein kinase C and elongation factor 1alpha. The four phylogenetic trees allowed to differentiate the black truffle clade, composed of two subclades, on...

journal_title：FEMS microbiology letters

authors： Wang Y,Tan ZM,Zhang DC,Murat C,Jeandroz S,Le Tacon F

更新日期：2006-06-01 00:00:00

abstract：:Ninety-three Salmonella isolates recovered from commercial foods and exotic animals in Colombia were studied. The serotypes, resistance profiles and where applicable the quinolone resistance genes were determined. Salmonella Anatum (n=14), Uganda (19), Braenderup (10) and Newport (10) were the most prevalent serovars,...

journal_title：FEMS microbiology letters

authors： Karczmarczyk M,Martins M,McCusker M,Mattar S,Amaral L,Leonard N,Aarestrup FM,Fanning S

更新日期：2010-12-01 00:00:00

abstract：:Airborne bacteria isolated from a tannery air environment were screened for the property of solubilization of insoluble zinc oxide and zinc phosphate. Out of 10 strains tested, a strain of Pseudomonas aeruginosa (CMG 823) showed the best solubilization and solubilized both zinc oxide and zinc phosphate. Colonies of th...

journal_title：FEMS microbiology letters

authors： Fasim F,Ahmed N,Parsons R,Gadd GM

更新日期：2002-07-16 00:00:00

abstract：:Acidithiobacillus ferrivorans is an acidophile implicated in low-temperature biomining for the recovery of metals from sulfide minerals. Acidithiobacillus ferrivorans obtains its energy from the oxidation of inorganic sulfur compounds, and genes encoding several alternative pathways have been identified. Next-generati...

journal_title：FEMS microbiology letters

authors： Christel S,Fridlund J,Buetti-Dinh A,Buck M,Watkin EL,Dopson M

更新日期：2016-04-01 00:00:00

abstract：:In the Pseudomonas fluorescens strain ST the ability to utilize styrene as the sole carbon source is due to the presence of a plasmid, pEG. In the present report we show that pEG contains two inverted repeat sequences and we present evidence indicating that the catabolic genes are localized in these repeats. The regio...

journal_title：FEMS microbiology letters

authors： Ruzzi M,Zennaro E

更新日期：1989-06-01 00:00:00

abstract：:A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

journal_title：FEMS microbiology letters

authors： Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

更新日期：2008-03-01 00:00:00

abstract：:Ruminococcus albus is a Gram-positive bacterium that degrades plant cell walls in the rumen of herbivores. It was described to synthesize two major glycoside-hydrolases (Cel9B and Cel48A), which are exported and anchored at the cell surface. In bacteria, proteins destined to cross the cytoplasmic membrane are synthesi...

journal_title：FEMS microbiology letters

authors： Esbelin J,Martin C,Forano E,Mosoni P

更新日期：2009-05-01 00:00:00

abstract：:Prebiotics are non-digestible feed ingredients that are metabolized by specific members of intestinal microbiota and provide health benefits for the host. Fermentable oligosaccharides are best known prebiotics that have received increasing attention in poultry production. They act through diverse mechanisms, such as p...

journal_title：FEMS microbiology letters

authors： Pourabedin M,Zhao X

更新日期：2015-08-01 00:00:00

abstract：:IS1(SB) and its two variants were identified as the major and minor IS1 elements in Shigella boydii. The nucleotide sequences of IS1(SB), IS1(O157:H7) from Escherichia coli O157:H7 and IS1F from E. coli K12 suggest that these IS1 elements had been horizontally transferred among S. boydii and E. coli O157:H7 and K12. T...

journal_title：FEMS microbiology letters

authors： Hsu WB,Chen JH

更新日期：2003-05-28 00:00:00