Purification and characterization of a membrane-associated ATPase from Natronococcus occultus, a haloalkaliphilic archaeon.


:Isolated membranes of the extreme haloalkaliphilic archaeon Natronococcus occultus were able to hydrolyze ATP via an ATPase, which required the presence of Mg(2+), high concentrations of NaCl, and a pH value of 9. The native molecular mass of the purified ATPase was 130 kDa and was composed of 74- and 61-kDa subunits. Enzyme activity was specific for the hydrolysis of ATP with slight activity towards GTP, CTP, and ITP. The enzyme required NaCl for maximal activity but Na(2)SO(4) and (NH(4))(2)SO(4) could substitute. The enzyme showed no activity if Na(2)SO(3) or sodium citrate was substituted for NaCl. The ATPase from N. occultus was inhibited by NBD-Cl, NaN(3), and ouabain, and was sensitive to nitrate, vanadate, DCCD, and bafilomycin A(1). It was not inhibited by NEM in contrast to other previously characterized halophile ATPases. The ATPase had a K(M) of 0.5 mM and appeared to be non-competitively inhibited by NaN(3) with a K(I) of 3.1 mM.


FEMS Microbiol Lett


Eddy ML,Jablonski PE





Has Abstract


2000-08-15 00:00:00














  • The Cyclic AMP-binding protein CbpB in Brucella melitensis and its role in cell envelope integrity, resistance to detergent and virulence.

    abstract::Brucella melitensis possesses an operon with two components: the response regulator OtpR and a putative cAMP-dependent protein kinase regulatory subunit encoded by the BMEI0067 gene. In the previous study, the function of OtpR has been studied, while little is known about the function of the BMEI0067 gene. Using a bio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Liu WJ,Dong H,Peng XW,Wu QM

    更新日期:2014-07-01 00:00:00

  • Biosynthesis of poly(4-hydroxybutyric acid) by recombinant strains of Escherichia coli.

    abstract::The aim of this study was the production of the homopolyester poly(4-hydroxybutyric acid) (poly(4HB)) with recombinant strains of Escherichia coli. Wild-type strains and other widely used non-recombinant strains of E. coli are not able to produce polyhydroxyalkanoic acids (PHA) as storage compounds and cannot utilize ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hein S,Söhling B,Gottschalk G,Steinbüchel A

    更新日期:1997-08-15 00:00:00

  • Purification and characterization of membrane-bound CO-reactive hemoprotein from Tetrahymena pyriformis mitochondria.

    abstract::A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Inokuchi A,Fukumori Y

    更新日期:1993-08-15 00:00:00

  • Borrelia burgdorferi possesses a collagenolytic activity.

    abstract::Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Grab DJ,Kennedy R,Philipp MT

    更新日期:1996-10-15 00:00:00

  • Clinical isolates of Pseudomonas aeruginosa from superficial skin infections have different physiological patterns.

    abstract::Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Buivydas A,Pasanen T,Senčilo A,Daugelavičius R,Vaara M,Bamford DH

    更新日期:2013-06-01 00:00:00

  • Alteration in the composition of cholesteryl glucosides and other lipids in Helicobacter pylori undergoing morphological change from spiral to coccoid form.

    abstract::In this analysis of membrane lipid compositions in Helicobacter pylori, the membrane lipid profiles drastically changed during coccoid formation: cholesteryl-6-O-tetradecanoyl-alpha-D-glucopyranoside levels increased, cholesteryl-alpha-D-glucopyranoside and phosphatidyl ethanolamine decreased, and a coordinated increa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shimomura H,Hayashi S,Yokota K,Oguma K,Hirai Y

    更新日期:2004-08-15 00:00:00

  • Metagenome microarray for screening of fosmid clones containing specific genes.

    abstract::A critical step in the process of metagenome analysis is to screen for clones that contain specific genes among a large number of clones. To form one of the sequence-based screening tools of a metagenome library, we designed a format of microarray [metagenome microarray (MGA)] that is arrayed with fosmid library clone...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Park SJ,Kang CH,Chae JC,Rhee SK

    更新日期:2008-07-01 00:00:00

  • A new root-nodulating symbiont of the tropical legume Sesbania, Rhizobium sp. SIN-1, is closely related to R. galegae, a species that nodulates temperate legumes.

    abstract::Rhizobium sp. SIN-1, isolated in India from root nodules on the tropical legume Sesbania aculeata, also induces nitrogen-fixing nodules on roots of S. macrocarpa, S. speciosa, S. procumbens, S. punicea, S. rostrata, and Vigna unguiculata. Unlike Azorhizobium caulinodans, SIN-1 does not induce stem nodules on S. rostra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rana D,Krishnan HB

    更新日期:1995-12-01 00:00:00

  • Roundup Ready soybean gene concentrations in field soil aggregate size classes.

    abstract::Roundup Ready (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Levy-Booth DJ,Gulden RH,Campbell RG,Powell JR,Klironomos JN,Pauls KP,Swanton CJ,Trevors JT,Dunfield KE

    更新日期:2009-02-01 00:00:00

  • Halogenation of aromatic compounds: thermodynamic, mechanistic and ecological aspects.

    abstract::Biological halogenation of aromatic compounds implies the generation of reducing equivalents in the form of e.g. NADH. Thermodynamic calculations show that coupling the halogenation step to a step in which the reducing equivalents are oxidized with a potent oxidant such as O2 or N2O makes the halogenation reaction the...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dolfing J

    更新日期:1998-10-15 00:00:00

  • Nicotinamide inhibits Plasmodium falciparum Sir2 activity in vitro and parasite growth.

    abstract::Plasmodium falciparum sirtuin, PfSir2, contains histone deacetylase (HDAC) activity that may be central to the regulation of virulence gene expression in the parasites. Although a few reports have been published recently regarding in vitro and in vivo function of PfSir2, expression of the endogenous protein (c. 30 kDa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Prusty D,Mehra P,Srivastava S,Shivange AV,Gupta A,Roy N,Dhar SK

    更新日期:2008-05-01 00:00:00

  • Bioplastic reservoir of diverse bacterial communities revealed along altitude gradient of Pangi-Chamba trans-Himalayan region.

    abstract::Himalaya hosts a unique environment for microbial ecology. The present study aimed to explore the bioplastic producing bacterial communities along altitude gradient of Pangi-Chamba trans-Himalayan region. A total of 411 bacteria were isolated and 70 characterized at the molecular level for potential polyhydroxyalkanoa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kumar V,Thakur V,Ambika,Kumar S,Singh D

    更新日期:2018-07-01 00:00:00

  • CpaA a novel protease from Acinetobacter baumannii clinical isolates deregulates blood coagulation.

    abstract::Acinetobacter baumannii is an important nosocomial pathogen that displays high antibiotic resistance. It causes a variety of infections including pneumonias and sepsis which may result in disseminated intravascular coagulation. In this work, we identify and characterize a novel secreted, zinc-dependent, metallo-endope...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tilley D,Law R,Warren S,Samis JA,Kumar A

    更新日期:2014-07-01 00:00:00

  • Mycoplasma gallisepticum rpoA gene cluster.

    abstract::Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Skamrov A,Feoktistova E,Goldman M,Beabealashvilli R

    更新日期:2002-03-05 00:00:00

  • DNA sequencing reveals bacterial communities in midgut and other parts of the larvae of Spodoptera exigua Hubner (Lepidoptera: Noctuidae).

    abstract::Gut microbiota has been suggested as an important component of host defense. However, microbiota in other parts of the body have rarely been characterized. In our present work, we give a detailed view of the body of Spodoptera exigua larvae, the midgut with intestinal contents (MG), midgut excluding intestinal content...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gao X,Li W,Luo J,Zhang L,Ji J,Zhu X,Wang L,Zhang K,Zhang S,Cui J

    更新日期:2020-02-01 00:00:00

  • An assay for exogenous sources of purified MurG, enabled by the complementation of Escherichia coli murG(Ts) by the Mycobacterium tuberculosis homologue.

    abstract::The Mycobacterium tuberculosis murG gene, Rv2153, was expressed in Escherichia coli murG(Ts) strain OV58 on a plasmid under the control of the arabinose-inducible araBAD promoter. Mycobacterium tuberculosis murG rescued the growth of E. coli murG(Ts) at the nonpermissive temperature: transformants were only obtained i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jha RK,Katagihallimath N,Hota SK,Das KS,de Sousa SM

    更新日期:2012-01-01 00:00:00

  • The 70 kilodalton iron regulated protein of Neisseria meningitidis is not the human transferrin receptor.

    abstract::Neisseria meningitidis is able to chelate iron from human transferrin (HTF), the main sequestrator of extracellular iron in vivo. Previous workers have reported that a ca. 70 kilodalton (kDa) iron regulated outer membrane protein (FeRP-70) is a highly specific receptor for HTF. We have examined the interaction between...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ala'Aldeen DA,Davies HA,Wall RA,Borriello SP

    更新日期:1990-05-01 00:00:00

  • Molecular analysis of enrichment cultures of marine ammonia oxidisers.

    abstract::Marine ammonia oxidising bacteria were enriched by incubation of sea water, amended with ammonium sulphate, and subsequent subculture in liquid inorganic medium. PCR primers were designed to be specific for rDNA sequences from ammonia oxidisers belonging to the beta-sub-group of the proteobacteria. These primers were ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: McCaig AE,Embley TM,Prosser JI

    更新日期:1994-07-15 00:00:00

  • MoST1 encoding a hexose transporter-like protein is involved in both conidiation and mycelial melanization of Magnaporthe oryzae.

    abstract::In a large-scale gene disruption screen of Magnaporthe oryzae, a gene MoST1 encoding a protein belonging to the hexose transporter family was identified as a gene required for conidiation and culture pigmentation. The gene MoST1 located on chromosome V of the M. oryzae genome was predicted to be 1892 bp in length with...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Saitoh H,Hirabuchi A,Fujisawa S,Mitsuoka C,Terauchi R,Takano Y

    更新日期:2014-03-01 00:00:00

  • Physicochemical modification of the excretion product of Saccharomyces cerevisiae killer strains results in fungicidal activity against Candida albicans and Tricophyton mentagrophytes.

    abstract::It is known that certain yeast strains, so called 'killers', can produce and excrete proteinaceous toxins that can induce death of other sensitive strains. We obtained a stable fungicidal factor (SKF) through concentration and stabilization of the excretion product of certain killer strains of Saccharomyces cerevisiae...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bracesco N,Salvo VA,Carrau FM,Nunes E

    更新日期:2006-03-01 00:00:00

  • Accumulation of ppGpp in Streptococcus pyogenes and Streptococcus rattus following amino acid starvation.

    abstract::We have re-examined the stringent response of Streptococcus rattus and Streptococcus pyogenes, two organisms that had originally been reported not to accumulate ppGpp following amino acid deprivation. We conclude that ppGpp does accumulate when S. rattus and S. pyogenes are deprived of isoleucine by mupirocin addition...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Whitehead KE,Webber GM,England RR

    更新日期:1998-02-01 00:00:00

  • Environmental adaptation factors of two yeasts isolated from the leachate of a uranium mineral heap.

    abstract::Two yeasts from the effluent of a uranium mineral heap were identified as Candida sorbophila and Rhodotorula mucilaginosa. They are well adapted to such an environment as shown by their tolerance to metals and acid pH. However, different mechanisms could be involved in the adaptation: C. sorbophila could be considered...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: de Silóniz MI,Payo EM,Callejo MA,Marquina D,Peinado JM

    更新日期:2002-05-07 00:00:00

  • Efficient screening for astaxanthin-overproducing mutants of the yeast Xanthophyllomyces dendrorhous by flow cytometry.

    abstract::Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ukibe K,Katsuragi T,Tani Y,Takagi H

    更新日期:2008-09-01 00:00:00

  • Isolation of the dxr gene of Zymomonas mobilis and characterization of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase.

    abstract::The gene encoding the second enzyme of the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway for isopentenyl diphosphate biosynthesis, 1-deoxy-D-xylulose 5-phosphate (DXP) reductoisomerase, was cloned and sequenced from Zymomonas mobilis. The deduced amino acid sequence showed the highest identity (48.2%) to the DXP re...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Grolle S,Bringer-Meyer S,Sahm H

    更新日期:2000-10-01 00:00:00

  • The rfaH gene, which affects lipopolysaccharide synthesis in Salmonella enterica serovar Typhi, is differentially expressed during the bacterial growth phase.

    abstract::We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi strain Ty2. The gene showed a high degree of similarity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rfaH mutant was constructed by site-directed mutagenesis. This mutant produced a rough lipopolysa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rojas G,Saldías S,Bittner M,Zaldívar M,Contreras I

    更新日期:2001-10-16 00:00:00

  • RD1 region in mycobacterial genome is involved in the induction of necrosis in infected RAW264 cells via mitochondrial membrane damage and ATP depletion.

    abstract::It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

    更新日期:2007-09-01 00:00:00

  • Diversity and distribution of hemerythrin-like proteins in prokaryotes.

    abstract::Hemerythrins are oxygen-binding proteins found in the body fluids and tissues of certain invertebrates. Oxygen is bound at a nonheme iron centre consisting of two oxo-bridged iron atoms bound to a characteristic set of conserved histidine: aspartate and glutamate residues with the motifs H-HxxxE-HxxxH-HxxxxD. It has r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: French CE,Bell JM,Ward FB

    更新日期:2008-02-01 00:00:00

  • Beta-glucosidase activity from the thermophilic fungus Scytalidium thermophilum is stimulated by glucose and xylose.

    abstract::An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively. The enzyme was stable up to 1 h at 50 degrees C a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zanoelo FF,Polizeli Mde L,Terenzi HF,Jorge JA

    更新日期:2004-11-15 00:00:00

  • Assessment of the serodiagnostic potential of nine novel proteins from Mycobacterium tuberculosis.

    abstract::To identify antigens that would improve the accuracy of serological diagnosis of active tuberculosis, we cloned the genes encoding nine potentially immunogenic secreted or surface-associated proteins of Mycobacterium tuberculosis. Recombinant proteins were reacted with sera from HIV-negative individuals with extrapulm...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Moran AJ,Treit JD,Whitney JL,Abomoelak B,Houghton R,Skeiky YA,Sampaio DP,Badaró R,Nano FE

    更新日期:2001-04-20 00:00:00

  • Functional expression of the glutamate uptake system from Corynebacterium glutamicum in Escherichia coli.

    abstract::Glutamate uptake in the Gram-positive Corynebacterium glutamicum is mediated via a binding protein-dependent transport system, which is encoded by the gluABCD gene cluster. Cloning of these genes in an expression vector and subsequent transformation of the resulting plasmid allows different strains of the Gram-negativ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Burkovski A,Krämer R

    更新日期:1995-04-01 00:00:00