Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis.

abstract：:Trypanosomatids are unicellular protozoan parasites that cause many diseases in animals, including humans, and plants. These early divergent eukaryotes have many singular structures and processes, including the hyper-modified 'base J', a mitochondrial DNA network, RNA editing, and trans-splicing; all of these unique f...

journal_title：FEMS microbiology letters

authors： Gargantini PR,Lujan HD,Pereira CA

更新日期：2012-10-01 00:00:00

abstract：:A cadmium-resistant strain of Saccharomyces cerevisiae produces a cadmium metallothionein encoded by the CUP1 gene as does a copper-resistant strain. The mechanism of expression of the gene is inducible by copper ions in the copper-resistant strain. However, assays of CUP1-specific mRNA revealed that the transcription...

journal_title：FEMS microbiology letters

authors： Tohoyama H,Inagawa A,Koike H,Inouhe M,Joho M,Murayama T

更新日期：1992-08-01 00:00:00

abstract：:Saccharomyces cerevisiae strains tolerant to osmotic stress are important for ethanol production during very high gravity (VHG) fermentation. We aimed to identify novel genes that confer enhanced tolerance to osmotic stress in S. cerevisiae. Two strains tolerant to up to 30% (w/v) glucose were isolated by screening a ...

journal_title：FEMS microbiology letters

authors： Kim B,Kim HS

更新日期：2018-07-01 00:00:00

abstract：:We developed a multiplex PCR to detect the presence of methicillin- (mecA), cadmium/zinc-(czrC) and antiseptic-resistant (qacA/B) staphylococci and to identify Panton-Valentine leukocidin (PVL)-positive and -negative Staphylococcus aureus and coagulase-negative staphylococci (CoNS) from infected and healthy eyes. The ...

journal_title：FEMS microbiology letters

authors： Panda S,Kar S,Choudhury R,Sharma S,Singh DV

更新日期：2014-03-01 00:00:00

abstract：:Mild alkaline solutions (beta-elimination), after removing the non-covalently bonded wall materials by hot SDS, released 13% and 26% of remaining wall proteins from mycelial and yeast cells of Candida albicans, respectively. When the beta-elimination was carried out after digestion of the walls with chitinase, four-fo...

journal_title：FEMS microbiology letters

authors： Mormeneo S,Marcilla A,Iranzo M,Sentandreu R

更新日期：1994-10-15 00:00:00

abstract：:The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) ...

journal_title：FEMS microbiology letters

authors： Edwards K,Linetsky I,Hueser C,Eisenstark A

更新日期：2001-05-30 00:00:00

abstract：:Enterococcus faecium, a major cause of nosocomial infections, is often isolated from conditions where biofilm is considered to be important in the establishment of infections. We investigated biofilm formation among E. faecium isolates from diverse sources and found that the occurrence and amount of biofilm formation ...

journal_title：FEMS microbiology letters

authors： Almohamad S,Somarajan SR,Singh KV,Nallapareddy SR,Murray BE

更新日期：2014-04-01 00:00:00

abstract：:The essential oil of German chamomile showed specific inhibition toward aflatoxin G(1) (AFG(1)) production, and (E)- and (Z)-spiroethers were isolated as the active compounds from the oil. The (E)- and (Z)-spiroethers inhibited AFG(1) production of Aspergillus parasiticus with inhibitory concentration 50% (IC(50)) val...

journal_title：FEMS microbiology letters

authors： Yoshinari T,Yaguchi A,Takahashi-Ando N,Kimura M,Takahashi H,Nakajima T,Sugita-Konishi Y,Nagasawa H,Sakuda S

更新日期：2008-07-01 00:00:00

abstract：:A Na(+)/H(+) antiporter gene was isolated from alkaliphilic Bacillus sp. G1. The full-length sequence of the Na(+)/H(+) antiporter gene was obtained using a genome walking method, and designated as g1-nhaC. An ORF preceded by a promoter-like sequence and a Shine-Dalgarno sequence, and followed by a terminator-like seq...

journal_title：FEMS microbiology letters

authors： Liew CW,Illias RM,Mahadi NM,Najimudin N

更新日期：2007-11-01 00:00:00

abstract：:A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

journal_title：FEMS microbiology letters

authors： Inokuchi A,Fukumori Y

更新日期：1993-08-15 00:00:00

abstract：:DNA microarray technology was used to survey changes in gene expression in Pseudomonas fluorescens after mitomycin C treatment. As expected, genes associated with the SOS response were upregulated, such as those encoding the recombination protein RecA, DNA repair protein RecN, excinuclease ABC subunit A UvrA, and the ...

journal_title：FEMS microbiology letters

authors： Jin H,Retallack DM,Stelman SJ,Hershberger CD,Ramseier T

更新日期：2007-04-01 00:00:00

abstract：:Pythium glomeratum sp. nov. is described here. It was isolated from soil samples taken in the northern France in 1992, was wrongly identified as Pythium heterothallicum and was kept aside. Recently, the ITS region of the rDNA of this oomycete was amplified and sequenced. The differences between the sequence and a more...

journal_title：FEMS microbiology letters

authors： Paul B

更新日期：2003-08-08 00:00:00

abstract：:The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracel...

journal_title：FEMS microbiology letters

authors： Larsen MH,Boesen HT

更新日期：2001-09-25 00:00:00

abstract：:We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from...

journal_title：FEMS microbiology letters

authors： Nübel U,Schmidt PM,Reiss E,Bier F,Beyer W,Naumann D

更新日期：2004-11-15 00:00:00

abstract：:Actinobacteria are known by their ability to produce several antimicrobial compounds of biotechnological interest. Thus, in this study, we isolated and identified by partial 16S RNA sequencing ∼100 actinobacteria isolates from guarana (Paullinia cupana) bulk soil. Besides, we isolated from the actinobacteria Streptomy...

journal_title：FEMS microbiology letters

authors： Vicente Dos Reis G,Abraham WR,Grigoletto DF,de Campos JB,Marcon J,da Silva JA,Quecine MC,de Azevedo JL,Ferreira AG,de Lira SP

更新日期：2019-07-01 00:00:00

abstract：:High voltage electroporation has been investigated as a method for rapid recovery of plasmid and chromosomal DNA from the cyanobacteria Nostoc PCC 7121, Synechococcus PCC 7002, and Anabaena PCC 7120. Pulses of 18 kV/cm and higher applied to concentrated Nostoc cells carrying a shuttle plasmid (pRL25) resulted in copio...

journal_title：FEMS microbiology letters

authors： Moser D,Zarka D,Hedman C,Kallas T

更新日期：1995-05-15 00:00:00

abstract：:The DnaK chaperone system is involved in various cellular processes such as the control of the folded and oligomeric state of proteins under stress and non-stress conditions. In this study we functionally characterised the homologues of the DnaK system from Clostridium acetobutylicum DnaK, DnaJ, GrpE and OrfA were het...

journal_title：FEMS microbiology letters

authors： Rüngeling E,Laufen T,Bahl H

更新日期：1999-01-01 00:00:00

abstract：:A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

journal_title：FEMS microbiology letters

authors： Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

更新日期：2008-03-01 00:00:00

abstract：:Piscirickettsia salmonis is a novel, aggressive, facultative Gram-negative bacterium that drastically affects salmon production at different latitudes, with particular impact in southern Chile. Initially, P. salmonis was described as a Rickettsia-like, obligate, intracellular Alphaproteobacteria, but it was reclassifi...

journal_title：FEMS microbiology letters

authors： Marshall SH,Henríquez V,Gómez FA,Cárdenas C

更新日期：2011-01-01 00:00:00

abstract：:A gene responsible for increased synthesis of hexaheme nitrite reductase (cytochrome c552) of Escherichia coli K-12 was cloned into pBR322 by the direct immunological screening method using antiserum against the purified enzyme. The cloned gene was mapped at 5 min on the chromosomal linkage map as the dni gene (relate...

journal_title：FEMS microbiology letters

authors： Kajie S,Ideta R,Yamato I,Anraku Y

更新日期：1991-10-01 00:00:00

abstract：:We have observed significant cell lysis upon temperature up-shift of recombinant Escherichia coli cultures harboring CI857-repressed lambda-based expression vectors. This event, that becomes evident about 30-40 min after the heat shock, takes place when using the lambda promoter system in Ind- lysogenic strains, but n...

journal_title：FEMS microbiology letters

authors： Hoffmann F,Arís A,Carbonell X,Rohde M,Corchero JL,Rinas U,Villaverde A

更新日期：1999-08-15 00:00:00

abstract：:Corynebacterium diphtheriae strains express non-fimbrial surface proteins able to recognize and bind to specific host cells receptors. Protein extracts were obtained from bacterial cells by mechanical process and ammonium sulfate precipitation at 25 and 45% (w/v) saturation. SDS-PAGE analysis of the extracts detected ...

journal_title：FEMS microbiology letters

authors： Colombo AV,Hirata R Jr,de Souza CM,Monteiro-Leal LH,Previato JO,Formiga LC,Andrade AF,Mattos-Guaraldi AL

更新日期：2001-04-13 00:00:00

abstract：:OMIZ-W68, a chemically defined medium that contains no long-chain fatty acids and yet supports in vitro proliferation of a wide range of fastidious oral anaerobes, is described. The type strains of Treponema denticola, Treponema lecithinolyticum, Treponema maltophilum, Treponema pectinovorum, Treponema socranskii, and...

journal_title：FEMS microbiology letters

authors： Wyss C

更新日期：2007-04-01 00:00:00

abstract：:The marine dinoflagellate genus Alexandrium includes a number of species that produce potent neurotoxins responsible for paralytic shellfish poisoning, which in humans may cause muscular paralysis, neurological symptoms and, in extreme cases, death. Because of the genetic diversity of different genera and species, mol...

journal_title：FEMS microbiology letters

authors： Wang L,Li L,Alam MJ,Geng Y,Li Z,Yamasaki S,Shi L

更新日期：2008-05-01 00:00:00

abstract：:Shigella strains are human pathogens and their identification is usually based on their O-antigens. The O-antigen gene cluster of Shigella boydii O11 was sequenced. All the expected genes for the synthesis of the O-antigen were identified on the basis of homology and genes for the biosynthesis of dTDP-l-Rhamnose, gene...

journal_title：FEMS microbiology letters

authors： Tao J,Feng L,Guo H,Li Y,Wang L

更新日期：2004-05-01 00:00:00

abstract：:Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by...

journal_title：FEMS microbiology letters

authors： Fang W,Luther DA,Oliver SP

更新日期：1998-09-15 00:00:00

abstract：:Salmonella enterica serovar Typhimurium is a food-borne pathogen and a leading cause of gastroenteritis in humans. Recently, we sequenced a phage-type DT108 strain (L945) and found reads with high similarity to both Salmonella typhi strain CT18 plasmid pHCM2 and bacteriophage SSU5. In this study, we completely sequenc...

journal_title：FEMS microbiology letters

authors： Octavia S,Sara J,Lan R

更新日期：2015-04-01 00:00:00

abstract：:A high-level expression system for the Escherichia coli HB101 gamma-glutamyltranspeptidase (GGT) gene was constructed to obtain a sufficient amount of the enzyme for structural studies. About 300 mg of the enzyme was purified from 2 1 of culture broth. Both subunits were needed to reconstitute GGT activity. The E. col...

journal_title：FEMS microbiology letters

authors： Ishiye M,Niwa M

更新日期：1992-10-15 00:00:00

abstract：:Fourteen solvent-sensitive transposon mutants were generated from the solvent-tolerant Pseudomonas putida strain S12 by applying the TnMod-KmO mutagenesis system. These mutants were unable to grow in the presence of octanol and toluene. By cloning the region flanking the transposon insertion point a partial sequence o...

journal_title：FEMS microbiology letters

authors： Kieboom J,Bruinenberg R,Keizer-Gunnink I,de Bont JA

更新日期：2001-05-01 00:00:00

abstract：:Mycobacteria belonging to the Mycobacterium tuberculosis complex have the ability to invade and replicate in non-phagocytic cells, an event that requires the presence of bacterial surface components capable of triggering a cell response and the subsequent internalization of the microorganism. In this study, we report ...

journal_title：FEMS microbiology letters

authors： Flesselles B,Anand NN,Remani J,Loosmore SM,Klein MH

更新日期：1999-08-15 00:00:00