Abstract:
:Saccharomyces cerevisiae strains tolerant to osmotic stress are important for ethanol production during very high gravity (VHG) fermentation. We aimed to identify novel genes that confer enhanced tolerance to osmotic stress in S. cerevisiae. Two strains tolerant to up to 30% (w/v) glucose were isolated by screening a transposon-mediated mutant library. Two genes were identified: TIS11 and SDS23. In addition, the ability of these genes to confer osmotic stress tolerance was demonstrated by disrupting and overexpressing the open reading frame of each gene. The two transposon mutants grew faster than the control strain in YPD rich medium containing 30% (w/v) glucose and showed activation of Hog1p in response to VHG glucose. The disruption of genes identified in this study, TIS11 and SDS23, provides a basis for improved tolerance to osmotic stress under VHG fermentation condition.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Kim B,Kim HSdoi
10.1093/femsle/fny149subject
Has Abstractpub_date
2018-07-01 00:00:00issue
14eissn
0378-1097issn
1574-6968pii
5040221journal_volume
365pub_type
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