Abstract:
:The aims of this work were to characterize the 16S-23S internal spacer region of the fish pathogen Tenacibaculum soleae and to develop a PCR assay for its identification and detection. All T. soleae strains tested displayed a single internal spacer region class, containing tRNA(I) (le) and tRNA(A) (la) genes; nevertheless, a considerable intraspecific heterogeneity was observed. However, this region proved to be useful for differentiation of T. soleae from related and non-related species. Species-specific primers were designed targeting the 16S rRNA gene and the internal spacer region region, yielding a 1555-bp fragment. Detection limit was of 1 pg DNA per reaction (< 30 bacterial cells) when using pure cultures. The detection level in the presence of DNA from fish or other bacteria was lower; however, 10 pg were detected at a target/background ratio of 1 : 10(5) . The PCR assay proved to be more sensitive than agar cultivation for the detection of T. soleae from naturally diseased fish, offering a useful tool for diagnosis and for understanding the epidemiology of this pathogen.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
López JR,Hamman-Khalifa AM,Navas JI,de la Herran Rdoi
10.1111/j.1574-6968.2011.02404.xsubject
Has Abstractpub_date
2011-11-01 00:00:00pages
181-8issue
2eissn
0378-1097issn
1574-6968journal_volume
324pub_type
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