Investigation of the role of ZurR in the physiology and pathogenesis of Listeria monocytogenes.

Abstract:

:Listeria monocytogenes is a Gram positive pathogen that is ubiquitous in the environment. It is a facultative anaerobic rod that causes listeriosis, a disease with potentially lethal consequences for susceptible individuals. During infection, the pathogen is capable of sequestering metal ions to act as vital biocatalysts in cellular processes. The zinc uptake regulator (ZurR) is predicted to coordinate uptake of zinc from the external environment. An in-frame deletion of the zurR gene resulted in a mutant exhibiting a small colony phenotype and a smaller cell size. The zurR mutant was unaffected under conditions of zinc limitation but demonstrated increased sensitivity to toxic levels of zinc. The mutant also demonstrated a significant (1-log) reduction in virulence potential in the murine model of infection. Using a bioinformatic approach, we identified a number of potentially Zur-regulated genes in the genome of L. monocytogenes. Quantitative RT-PCR demonstrated significant de-repression of zurA, lmo0153, and lmo1671 in the zurR mutant background indicating that these putative transporters are ZurR regulated.

journal_name

FEMS Microbiol Lett

authors

Dowd GC,Casey PG,Begley M,Hill C,Gahan CG

doi

10.1111/j.1574-6968.2011.02472.x

subject

Has Abstract

pub_date

2012-02-01 00:00:00

pages

118-25

issue

2

eissn

0378-1097

issn

1574-6968

journal_volume

327

pub_type

杂志文章
  • Characterisation of the activator effect of glycogen on amylosucrase from Neisseria polysaccharea.

    abstract::Amylosucrase produces an insoluble alpha-1,4-linked glucan from sucrose, releasing fructose. In addition to polymerisation, in the presence of sucrose as sole substrate, amylosucrase catalyses sucrose hydrolysis and oligosaccharide synthesis in significant proportions. The effects of both glycogen acceptor and sucrose...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09089.x

    authors: Potocki de Montalk G,Remaud-Simeon M,Willemot RM,Monsan P

    更新日期:2000-05-01 00:00:00

  • Exploration of deep intraterrestrial microbial life: current perspectives.

    abstract::Intraterrestrial life has been found at depths of several thousand metres in deep sub-sea floor sediments and in the basement crust beneath the sediments. It has also been found at up to 2800-m depth in continental sedimentary rocks, 5300-m depth in igneous rock aquifers and in fluid inclusions in ancient salt deposit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2000.tb09033.x

    authors: Pedersen K

    更新日期:2000-04-01 00:00:00

  • Role of the 5'-UTR in accumulation of the rbpA1 transcript at low temperature in the cyanobacterium Anabaena variabilis M3.

    abstract::The expression of the rbp genes, which encode RNA-binding proteins with a single RNA-recognition motif and a glycine-rich sequence, is known to increase at low temperature in cyanobacteria. We previously showed that their regulation involved both transcription and mRNA stability. In the present study, various reporter...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.07.034

    authors: Ehira S,Ohmori M,Sato N

    更新日期:2005-10-01 00:00:00

  • MoST1 encoding a hexose transporter-like protein is involved in both conidiation and mycelial melanization of Magnaporthe oryzae.

    abstract::In a large-scale gene disruption screen of Magnaporthe oryzae, a gene MoST1 encoding a protein belonging to the hexose transporter family was identified as a gene required for conidiation and culture pigmentation. The gene MoST1 located on chromosome V of the M. oryzae genome was predicted to be 1892 bp in length with...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12369

    authors: Saitoh H,Hirabuchi A,Fujisawa S,Mitsuoka C,Terauchi R,Takano Y

    更新日期:2014-03-01 00:00:00

  • Cloning and sequencing of a dextranase-encoding cDNA from Penicillium minioluteum.

    abstract::A cDNA from Penicillium minioluteum HI-4 encoding a dextranase (1,6-alpha-glucan hydrolase, EC 3.2.1.11) was isolated and characterized. cDNA clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. Southern hybridization and restriction mapping analysis of sel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08477.x

    authors: Garcia B,Margolles E,Roca H,Mateu D,Raices M,Gonzales ME,Herrera L,Delgado J

    更新日期:1996-10-01 00:00:00

  • The emergence of colistin-resistant Escherichia coli in chicken meats in Nepal.

    abstract::The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz237

    authors: Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

    更新日期:2019-10-01 00:00:00

  • Production in vitro, on different solid culture media, of two distinct exopolysaccharides by a mucoid clinical strain of Burkholderia cepacia.

    abstract::The production of exopolysaccharides (EPSs) by a mucoid clinical isolate of Burkholderia cepacia involved in infections in cystic fibrosis patients, was studied. Depending on the growth conditions, this strain was able to produce two different EPS, namely PS-I and PS-II, either alone or together. PS-I is composed of e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10792.x

    authors: Cérantola S,Montrozier H

    更新日期:2001-08-07 00:00:00

  • General and kinetic properties of endoglucanase from Aspergillus niger.

    abstract::Endoglucanase of Aspergillus niger AS-101 was partially purified by ammonium sulphate fractionation and molecular sieving on Sephadex G-200. The enzyme was found to be unstable on storage, however, it received some protection on the addition of BSA, glycerol or sodium azide. Glycerol also protected the enzyme from ina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90060-4

    authors: Singh A,Agrawal AK,Abidi AB,Darmwal NS

    更新日期:1990-09-01 00:00:00

  • Molecular host mimicry and manipulation in bacterial symbionts.

    abstract::It is common among intracellular bacterial pathogens to use eukaryotic-like proteins that mimic and manipulate host cellular processes to promote colonization and intracellular survival. Eukaryotic-like proteins are bacterial proteins with domains that are rare in bacteria, and known to function in the context of a eu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnz038

    authors: Frank AC

    更新日期:2019-02-01 00:00:00

  • Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum.

    abstract::A Gram-negative, nonmotile and rod-shaped bacterial strain was isolated from the rhizosphere of Platycodon grandiflorum in a study of bacterial diversity, and its taxonomic position was investigated by a genotypic and phenotypic analysis. This isolate, designated as DR-f4, grew at 4-30 degrees C (optimally at 20-25 de...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02026.x

    authors: Kim BC,Lee KH,Kim MN,Lee J,Shin KS

    更新日期:2010-08-01 00:00:00

  • Unexpected connections between type VI-B CRISPR-Cas systems, bacterial natural competence, ubiquitin signaling network and DNA modification through a distinct family of membrane proteins.

    abstract::In addition to core Cas proteins, CRISPR-Cas loci often encode ancillary proteins that modulate the activity of the respective effectors in interference. Subtype VI-B1 CRISPR-Cas systems encode the Csx27 protein that down-regulates the activity of Cas13b when the type VI-B locus is expressed in Escherichia coli. We sh...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz088

    authors: Makarova KS,Gao L,Zhang F,Koonin EV

    更新日期:2019-04-01 00:00:00

  • Microbial organic acid production as carbon dioxide sink.

    abstract::Mixed-substrate conversions are an under-regarded option to fix carbon dioxide in significant amounts. In such a conversion, carbon dioxide together with one other carbon source such as glucose is converted to a single carbon product. With mixed-substrate conversions, it is possible to incorporate carbon dioxide into ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx212

    authors: Steiger MG,Mattanovich D,Sauer M

    更新日期:2017-11-15 00:00:00

  • Action of Escherichia coli heat-stable enterotoxin II on isolated sections of mouse ileum.

    abstract::When Escherichia coli STII was applied to the serosa of the ileum at a concentration of 40 micrograms/ml, an acceleration of the spontaneous motility and a weak contraction were induced 2-3 min later. The induction was not affected by the addition of atropine (10(-6) M), but was abolished by the addition of papaverine...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90655-8

    authors: Hitotsubashi S,Akagi M,Saitou A,Yamanaka H,Fujii Y,Okamoto K

    更新日期:1992-01-15 00:00:00

  • Regulation of the yeast vacuolar aminopeptidase Y gene (APY1) expression.

    abstract::The steady-state levels of the aminopeptidase Y (APY1) transcript and the level of assayable aminopeptidase Y activity were measured under a variety of nutritional conditions. Specific APY1 mRNA is less abundant in cells grown in minimal medium than in cells grown in rich medium, while active enzyme levels follow just...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08191.x

    authors: Herrera-Camacho I,Suárez-Rendueles P

    更新日期:1996-06-01 00:00:00

  • The regulatory effect of fermentable sugar levels on the production of leukotoxin by Actinobacillus actinomycetemcomitans.

    abstract::The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited ch...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10187.x

    authors: Mizoguchi K,Ohta H,Miyagi A,Kurihara H,Takashiba S,Kato K,Murayama Y,Fukui K

    更新日期:1997-01-01 00:00:00

  • Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs.

    abstract::Eight-day-old embryonated hen's eggs were used as a model to study Mycobacterium avium virulence. Strains isolated from human patients caused 20-90% mortality when eggs were infected by injection of bacterial suspensions into the amniotic sac. Virulence of examined strains subsequently decreased with passage through e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09297.x

    authors: Long EG,Ewing EP Jr,Bartlett JH,Horsburgh CR Jr,Birkness KA,Yakrus MA,Newman GW,Quinn FD

    更新日期:2000-09-15 00:00:00

  • Use of a lux reporter system for monitoring rapid changes in alpha-toxin gene expression in Clostridium perfringens during growth.

    abstract::To determine whether the luxA-luxB reporter system is suitable as a sensitive reporter for rapid real-time measurements of alpha-toxin gene (cpa) expression in Clostridium perfringens, and to widen the range of alpha-toxin-producing C. perfringens strains examined with respect to cpa expression during growth, the repo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09164.x

    authors: Phillips-Jones MK

    更新日期:2000-07-01 00:00:00

  • Biosynthesis of poly(4-hydroxybutyric acid) by recombinant strains of Escherichia coli.

    abstract::The aim of this study was the production of the homopolyester poly(4-hydroxybutyric acid) (poly(4HB)) with recombinant strains of Escherichia coli. Wild-type strains and other widely used non-recombinant strains of E. coli are not able to produce polyhydroxyalkanoic acids (PHA) as storage compounds and cannot utilize ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb12604.x

    authors: Hein S,Söhling B,Gottschalk G,Steinbüchel A

    更新日期:1997-08-15 00:00:00

  • Successful enrichment of low-abundant comammox Nitrospira from nitrifying granules under ammonia-limited conditions.

    abstract::In artificial engineered systems, nitrification is a key reaction that accounts for the removal of biological nitrogen. Recently, a single microbe capable of oxidizing ammonia to nitrate, known as a complete ammonia oxidizer (comammox), has been discovered. Although the abundance and diversity of comammox Nitrospira i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa025

    authors: Fujitani H,Nomachi M,Takahashi Y,Hasebe Y,Eguchi M,Tsuneda S

    更新日期:2020-01-01 00:00:00

  • Delineation of species within the Trichoderma viride/atroviride/koningii complex by UP-PCR cross-blot hybridization.

    abstract::Sequences from the ribosomal DNA ITS regions have shown that there is limited variation among the species in the Trichoderma viride/atroviride/koningii species complex also known as Hypocrea rufa complex, and that infraspecies variation sometimes is larger than interspecies variation. Strains belonging to T. viride, T...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.06.041

    authors: Lübeck M,Bulat S,Alekhina I,Lieckfeldt E

    更新日期:2004-08-15 00:00:00

  • Prevalence of the multiple antibiotic resistance operon (marRAB) in the genus Salmonella.

    abstract::The multiple antibiotic resistance operon (marRAB) is a member of the multidrug resistance (mdr) systems. Similar to other mdr systems, this operon when induced encodes resistance to structurally and functionally unrelated antibiotics. marRAB has been shown to be conserved in the family Enterobacteriaceae, but within ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09153.x

    authors: Kunonga NI,Sobieski RJ,Crupper SS

    更新日期:2000-06-15 00:00:00

  • Roseovarius sp. strain 217: aerobic taurine dissimilation via acetate kinase and acetate-CoA ligase.

    abstract::The genome sequence of Roseovarius sp. strain 217 indicated that many pathway enzymes found in other organisms for the degradation of taurine are represented, but that a novel, apparently energy-dependent pathway is involved in the conversion of acetyl phosphate to acetyl CoA. Thus, an ABC transporter for taurine coul...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00719.x

    authors: Baldock MI,Denger K,Smits TH,Cook AM

    更新日期:2007-06-01 00:00:00

  • Decolourisation of synthetic textile dyes by Phlebia tremellosa.

    abstract::Phlebia tremellosa decolourised eight synthetic textile dyes (200 mg l(-1)) by greater than 96% within 14 days under stationary incubation conditions. High performance liquid chromatography analysis of culture supernatants indicated that Remazol Black B was degraded by the fungus, however, complete mineralisation did ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09174.x

    authors: Kirby N,Marchant R,McMullan G

    更新日期:2000-07-01 00:00:00

  • Effect of coumarin on glucose uptake by anaerobic rumen fungi in the presence and absence of Methanobrevibacter smithii.

    abstract::The effect of coumarin (1,2 benzopyrone) on glucose utilisation by the anaerobic rumen fungi Neocallimastix frontalis and N. patriciarum has been compared with the effect of p-coumaric acid. Both compounds largely inhibited glucose utilisation by N. patriciarum strain Cx when present in the medium at a concentration o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1990.tb13970.x

    authors: Cansunar E,Richardson AJ,Wallace G,Stewart CS

    更新日期:1990-07-01 00:00:00

  • A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita.

    abstract::To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnos...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11262.x

    authors: Yokoyama K,Tateishi T,Marumoto T,Saito M

    更新日期:2002-07-02 00:00:00

  • SshAI restriction endonuclease from Salmonella shikmonah.

    abstract::A new type II restriction endonuclease, SshAI, was purified from Salmonella shikmonah TK139 of kangaroo origin. The recognition and cleavage specificity of Ssh AI was determined to be 5'-CC/TNAGG-3', identical to that of SauI from Streptomyces aureofaciens and Bsu36I from Bacillus subtilis. Based on closely related an...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90291-w

    authors: Miyahara M,Nakajima K,Kawanishi T,Mise K

    更新日期:1990-01-01 00:00:00

  • Evidence for 1,8-dihydroxynaphthalene melanin in three halophilic black yeasts grown under saline and non-saline conditions.

    abstract::The ascomycetous black yeasts Hortaea werneckii, Phaeotheca triangularis, and Trimmatostroma salinum are halophilic fungi that inhabit hypersaline water of solar salterns. They are characterized by slow, meristematic growth and very thick, darkly pigmented cell walls. The dark pigment, generally thought to be melanin,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(04)00073-4

    authors: Kogej T,Wheeler MH,Lanisnik Rizner T,Gunde-Cimerman N

    更新日期:2004-03-19 00:00:00

  • The triazine hydrolase gene trzN from Nocardioides sp. strain C190: cloning and construction of gene-specific primers.

    abstract::Using oligonucleotides derived from the N-terminal sequence of a triazine hydrolase from Nocardioides sp. strain C190, two DNA fragments containing trzN were cloned into Escherichia coli and their nucleotide sequences were determined. The 456-amino acid polypeptide predicted from the 1356-bp trzN ORF displayed signifi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb10989.x

    authors: Mulbry WW,Zhu H,Nour SM,Topp E

    更新日期:2002-01-02 00:00:00

  • Interaction of dietary resveratrol with animal-associated bacteria.

    abstract::Resveratrol (3,5,4'-trihydroxy-trans-stilbene), an antifungal phytoalexin produced by grapes, peanuts, and Japanese knotweeds, is thought to be a beneficial dietary phytochemical in red wine and grape juice. Information on its antibacterial properties and biotransformation, however, is limited. We surveyed the interac...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01691.x

    authors: Jung CM,Heinze TM,Schnackenberg LK,Mullis LB,Elkins SA,Elkins CA,Steele RS,Sutherland JB

    更新日期:2009-08-01 00:00:00

  • Analysis of hybrids of Candida albicans formed by protoplast fusion.

    abstract::A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06617.x

    authors: Law C,Kavanagh K,Whittaker P

    更新日期:1994-01-01 00:00:00