Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs.

Abstract:

:Eight-day-old embryonated hen's eggs were used as a model to study Mycobacterium avium virulence. Strains isolated from human patients caused 20-90% mortality when eggs were infected by injection of bacterial suspensions into the amniotic sac. Virulence of examined strains subsequently decreased with passage through eggs to between 0 and 40% mortality in four passages. Virulence of the egg-attenuated strains could be restored by passage through human peripheral blood mononuclear cells. The site of infection in the egg was usually the mesodermal layer of the chorioallantoic membrane. A few small granulomas containing acid-fast bacteria were seen in the liver, but not in other organs. Death of chicken embryos may have resulted from destruction of the mesodermal layer of the chorioallantoic membrane with consequent respiratory failure. PBMCs infected with less virulent egg-passaged strains of M. avium produced higher levels of tumor necrosis factor-alpha than did peripheral blood mononuclear cells infected with more virulent nonpassaged strains.

journal_name

FEMS Microbiol Lett

authors

Long EG,Ewing EP Jr,Bartlett JH,Horsburgh CR Jr,Birkness KA,Yakrus MA,Newman GW,Quinn FD

doi

10.1111/j.1574-6968.2000.tb09297.x

keywords:

subject

Has Abstract

pub_date

2000-09-15 00:00:00

pages

267-72

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(00)00346-3

journal_volume

190

pub_type

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