Degradation of homovanillate by a strain of Variovorax paradoxus via ring hydroxylation.

Abstract:

:A newly isolated strain of Variovorax paradoxus could grow on homovanillate and several monohydroxylated phenylacetic acids. During growth on homovanillate, the organism formed separate NAD(P)H-dependent hydroxylases with activity towards 4-hydroxyphenylacetic acid and homovanillate. Homovanillate hydroxylase catalysed a typical monooxygenase reaction and had little activity towards 4-hydroxyphenylacetic acid GC-MS and TLC analysis suggested that homovanillate was 1-hydroxylated to yield a dihydroxymonomethoxyphenylacetic acid which served as a substrate for homogentisate 1,2-dioxygenase. Methanol, but not formaldehyde, was released either during ring-cleavage or subsequent metabolism of the ring-cleavage product.

journal_name

FEMS Microbiol Lett

authors

Allison N,Turner JE,Wait R

doi

10.1111/j.1574-6968.1995.tb07940.x

subject

Has Abstract

pub_date

1995-12-15 00:00:00

pages

213-9

issue

2-3

eissn

0378-1097

issn

1574-6968

journal_volume

134

pub_type

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