Degradation of homovanillate by a strain of Variovorax paradoxus via ring hydroxylation.


:A newly isolated strain of Variovorax paradoxus could grow on homovanillate and several monohydroxylated phenylacetic acids. During growth on homovanillate, the organism formed separate NAD(P)H-dependent hydroxylases with activity towards 4-hydroxyphenylacetic acid and homovanillate. Homovanillate hydroxylase catalysed a typical monooxygenase reaction and had little activity towards 4-hydroxyphenylacetic acid GC-MS and TLC analysis suggested that homovanillate was 1-hydroxylated to yield a dihydroxymonomethoxyphenylacetic acid which served as a substrate for homogentisate 1,2-dioxygenase. Methanol, but not formaldehyde, was released either during ring-cleavage or subsequent metabolism of the ring-cleavage product.


FEMS Microbiol Lett


Allison N,Turner JE,Wait R




Has Abstract


1995-12-15 00:00:00












  • An endophytic/pathogenic Phoma sp. from creosote bush producing biologically active volatile compounds having fuel potential.

    abstract::A Phoma sp. was isolated and characterized as endophytic and as a pathogen of Larrea tridentata (creosote bush) growing in the desert region of southern Utah, USA. This fungus produces a unique mixture of volatile organic compounds (VOCs), including a series of sesquiterpenoids, some alcohols and several reduced napht...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Strobel G,Singh SK,Riyaz-Ul-Hassan S,Mitchell AM,Geary B,Sears J

    更新日期:2011-07-01 00:00:00

  • Fine structure of hepatitis B virus surface antigen produced by recombinant yeast: comparison with HBsAg of human origin.

    abstract::The ultrastructure of hepatitis B virus surface antigen (HBsAg) particles produced by recombinant yeast cells was examined using high-resolution negative staining, and ice embedding, electron microscopy. With negative staining, the HBsAg particles were spherical to slightly ovoid with a mean diameter of 27.5 nm and co...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yamaguchi M,Sugahara K,Shiosaki K,Mizokami H,Takeo K

    更新日期:1998-08-15 00:00:00

  • The effect of buthionine sulfoximine on the growth of Leishmania donovani in culture.

    abstract::Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

    更新日期:1999-04-01 00:00:00

  • Characterization of Xanthomonas oryzae pv. oryzae recX, a gene that is required for high-level expression of recA.

    abstract::Analysis of the nucleotide sequence downstream from the Xanthomonas oryzae pv. oryzae recA gene reveals two orfs designated orfX and recX. The former has the potential to code for a 5.6 kDa protein of unknown function while the latter encodes for a putative 14.6 kDa protein with homology to RecX from various bacteria....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sukchawalit R,Vattanaviboon P,Utamapongchai S,Vaughn G,Mongkolsuk S

    更新日期:2001-11-27 00:00:00

  • Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects.

    abstract::A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

    更新日期:2008-03-01 00:00:00

  • Deacetoxycephalosporin C synthase isozymes exhibit diverse catalytic activity and substrate specificity.

    abstract::The biosynthesis of cephalosporins involving a thiozolidine ring expansion is catalyzed by deacetoxycephalosporin C synthase (DAOCS). In this study, three DAOCS isozymes were cloned and expressed as active enzymes together with Streptomyces jumonjinensis DAOCS that was newly isolated and partially characterized. The e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chin HS,Sim J,Seah KI,Sim TS

    更新日期:2003-01-28 00:00:00

  • Light-activated adenyl cyclase from Trichoderma viride.

    abstract::The effect of light on adenyl cyclase (E.C. and 3':5'-cyclic-AMP-phosphodiesterase (E.C. activity of Trichoderma viride was investigated. Adenyl cyclase proved to be a membrane-associated enzyme, requiring Mn2+ and was activated by light. In contrast, 3':5'-cyclic-AMP-phosphodiesterase showed no lig...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kolarova N,Haplová J,Gresík M

    更新日期:1992-06-15 00:00:00

  • Characterization of a novel plasmid from extremely halophilic Archaea: nucleotide sequence and function analysis.

    abstract::We determined the complete nucleotide sequence of the 16341 bp plasmid pHH205 of the extremely halophilic archaeon Halobacterium salinarum J7. The plasmid has a G+C content of 61.1%. A number of direct and inverted repeat sequences were found in pHH205, while no insertion sequences were found. Thirty-eight large open ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ye X,Ou J,Ni L,Shi W,Shen P

    更新日期:2003-04-11 00:00:00

  • Horizontal transfer of nonconjugative plasmids in a colony biofilm of Escherichia coli.

    abstract::We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm of mixed Escherichia coli strains. By simply coculturing a plasmid-free F(-) strain and another F(-) strain harboring a nonconjugative plasmid in a colony biofilm on antibiotic-free agar media, transformed cells were produced within 2...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Maeda S,Ito M,Ando T,Ishimoto Y,Fujisawa Y,Takahashi H,Matsuda A,Sawamura A,Kato S

    更新日期:2006-02-01 00:00:00

  • Disruption of response regulator gene, devR, leads to attenuation in virulence of Mycobacterium tuberculosis.

    abstract::The devR-devS two-component system of Mycobacterium tuberculosis was identified earlier and partially characterized in our laboratory. A devR::kan mutant of M. tuberculosis was constructed by allelic exchange. The devR mutant strain showed reduced cell-to-cell adherence in comparison to the parental strain in laborato...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Malhotra V,Sharma D,Ramanathan VD,Shakila H,Saini DK,Chakravorty S,Das TK,Li Q,Silver RF,Narayanan PR,Tyagi JS

    更新日期:2004-02-16 00:00:00

  • Purification and characterization of an exo-polygalacturonase from the tomato vascular wilt pathogen Fusarium oxysporum f.sp. lycopersici.

    abstract::An exo-polygalacturonase (EC was purified to apparent homogeneity from cultures of Fusarium oxysporum f.sp. lycopersici on synthetic medium supplemented with citrus pectin, using preparative isoelectric focusing. The enzyme, denominated PG2, had an apparent M(r) of 74000 Da upon SDS-PAGE. The pI of the main ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Di Pietro A,Roncero MI

    更新日期:1996-12-01 00:00:00

  • Genetic investigation within Lactococcus garvieae revealed two genomic lineages.

    abstract::The diversity of a collection of 49 Lactococcus garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses and a multilocus restriction typing (MLRT) carried out on six partial genes (at...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Ferrario C,Ricci G,Borgo F,Rollando A,Fortina MG

    更新日期:2012-07-01 00:00:00

  • High-frequency occurrence of virus-like particles with double-stranded RNA genome in Fusarium poae.

    abstract::Fifty-five geographically different strains of Fusarium poae were assayed for the presence of extrachromosomal nucleic acid elements. All strains were found to harbour double-stranded RNA (dsRNA) elements and encapsidated virus-like particles (VLP). There were great individual differences in dsRNA patterns of the vari...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fekete C,Giczey G,Papp I,Szabó L,Hornok L

    更新日期:1995-09-15 00:00:00

  • Supersusceptibility to hydrophobic antimicrobial agents and cell surface hydrophobicity in Branhamella catarrhalis.

    abstract::To clarify the cause of the supersusceptibility of Branhamella catarrhalis to macrolide antibiotics, which are well-known to be inactive to most Gram-negative bacteria, we determined its cell surface hydrophobicity by the partition experiment between water and hydrocarbons. Its cell surface was found to be markedly mo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gotoh N,Tanaka S,Nishino T

    更新日期:1989-05-01 00:00:00

  • Epitope mapping by cysteine mutagenesis: identification of residues involved in recognition by three monoclonal antibodies directed against LamB glycoporin in the outer membrane of Escherichia coli.

    abstract::Site-directed mutagenesis of the lamB gene was used to introduce individual cysteine substitutions at 20 sites in two regions (surface loops L7 and L8) of LamB protein significant in antibody recognition. Characterisation of cysteine mutants involved immunoblotting with three surface-specific monoclonal antibodies (mA...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Notley L,Hillier C,Ferenci T

    更新日期:1994-07-15 00:00:00

  • Involvement of O8-antigen in altering beta-lactam antibiotic susceptibilities in Escherichia coli.

    abstract::In spite of being dispensable, O-antigens are believed to facilitate various cellular processes and alter antibiotic sensitivities. Escherichia coli K-12 (CS109) strains are lacking in O-antigens and are reported to be sensitive to antibiotics. To our surprise, E. coli 2443 (expressing O8-antigen) manifested two- to f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sarkar SK,Ghosh AS

    更新日期:2008-05-01 00:00:00

  • Ability of human sera to neutralise the activity of Vero cytotoxins VT1, VT2 and variant forms of VT2.

    abstract::Sera, from 17 patients with diarrhoea or haemolytic uraemic syndrome, and six healthy adults, were tested for neutralisation of Vero cytotoxins (VT). For all 17 patients there was evidence of infection with Escherichia coli O157. Sera from two controls but from none of the patients neutralised VT1, although two patien...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Scotland SM,Said B,Thomas A,Rowe B

    更新日期:1994-01-15 00:00:00

  • Implication of a regulator of G protein signalling (BbRGS1) in conidiation and conidial thermotolerance of the insect pathogenic fungus Beauveria bassiana.

    abstract::Beauveria bassiana is an insect pathogenic fungus widely used as a biocontrol agent to infect and control insect pests. The conidium initiates pathogenesis and mediates disease transmission, however, little is known about genetic elements that control conidiation. Here, the cloning and characterization of a regulatory...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fang W,Scully LR,Zhang L,Pei Y,Bidochka MJ

    更新日期:2008-02-01 00:00:00

  • Plasmid-mediated virulence genes in non-typhoid Salmonella serovars.

    abstract::Specific non-typhoid Salmonella serovars carry large virulence plasmids that promote sustained extra-intestinal infections. These plasmids all share a highly conserved 8-kb region containing the spv operon, consisting of the regulatory spvR locus and the four structural spvABCD genes. The SpvR protein belongs to the L...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Guiney DG,Fang FC,Krause M,Libby S

    更新日期:1994-11-15 00:00:00

  • Green fluorescent protein-cell wall fusion proteins are covalently incorporated into the cell wall of Saccharomyces cerevisiae.

    abstract::Tagging of two cell wall mannoproteins, Cwp1p and Cwp2p, in Saccharomyces cerevisiae with the green fluorescent protein from Aequorea victoria resulted in incorporation of fluorescent fusion proteins into the cell wall. Both living cells and isolated cell walls became brightly labeled. Intriguingly, the incorporation ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ram AF,Van den Ende H,Klis FM

    更新日期:1998-05-15 00:00:00

  • An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda Red.

    abstract::We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, lambda Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by lambda Red and large deletions of both 117- and 165-kbp regions could be gener...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fukiya S,Mizoguchi H,Mori H

    更新日期:2004-05-15 00:00:00

  • Antibacterial action of acriflavine hydrochloride for eradication of the gastric pathogen Helicobacter pylori.

    abstract::Helicobacter pylori, a type 1 carcinogen, accounts for numerous gastric cancer-related deaths worldwide. Repurposing existing drugs or developing new ones for a combinatorial approach against increasing antimicrobial resistance is the need of the hour. This study highlights the efficacy of acriflavine hydrochloride (A...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tehlan A,Karmakar BC,Paul S,Kumar R,Kaur I,Ghosh A,Mukhopadhyay AK,Dhar SK

    更新日期:2020-11-23 00:00:00

  • The tolC locus affects the expression of sbmA through σE activity increase.

    abstract::The SbmA protein is involved in the transport of MccB17-, MccJ25-, bleomycin- and proline-rich peptides into the Escherichia coli cytoplasm. sbmA gene homologues were found in a variety of bacteria. However, the physiological role of this protein still remains unknown. Previously, we found that a combination of sbmA a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Corbalán NS,Adler C,de Cristóbal RE,Pomares MF,Delgado MA,Vincent PA

    更新日期:2010-10-01 00:00:00

  • Metabolomics approach used for understanding temperature-related pectinase activity in Bacillus licheniformis DY2.

    abstract::Pectinases are enzymes that catalyze pectin degradation. There is a global demand for pectinases because of their wide utility and catalytic efficiency. Optimization of the fermentation process to increase the pectolytic enzyme activity is generally practiced to lower process costs, but whether temperature influences ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Guan Y,Yin D,Du X,Ye X

    更新日期:2018-12-01 00:00:00

  • Rapid identification of marine bioluminescent bacteria by amplified 16S ribosomal RNA gene restriction analysis.

    abstract::To rapidly identify natural isolates of marine bioluminescent bacteria, we developed amplified ribosomal DNA restriction analysis (ARDRA) methods. ARDRA, which is based on the restriction patterns of 16S rRNA gene digested with five enzymes (EcoRI, DdeI, HhaI, HinfI, RsaI), clearly distinguished the 14 species of mari...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kita-Tsukamoto K,Wada M,Yao K,Kamiya A,Yoshizawa S,Uchiyama N,Kogure K

    更新日期:2006-03-01 00:00:00

  • Comparison of in vivo and in vitro properties of capsulated and noncapsulated variants of Mycoplasma mycoides subsp. mycoides strain Afadé: a potential new insight into the biology of contagious bovine pleuropneumonia.

    abstract::Mycoplasma mycoides subsp. mycoides (Mmm) strain Afadé had previously been shown to undergo spontaneous phase variations between an opaque capsulated variant and a translucent (TR) variant devoid of a capsule but able to secrete cell-free exopolysaccharides. This phase variation is associated with an ON/OFF genetic sw...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gaurivaud P,Lakhdar L,Le Grand D,Poumarat F,Tardy F

    更新日期:2014-10-01 00:00:00

  • Novel putative rhizobial species with different symbiovars nodulate Lotus creticus and their differential preference to distinctive soil properties.

    abstract::Phylogenetically diverse rhizobial strains endemic to Tunisia were isolated from symbiotic nodules of Lotus creticus, growing in different arid extremophile geographical regions of Tunisia, and speciated using multiloci-phylogenetic analysis as Neorhizobium huautlense (LCK33, LCK35, LCO42 and LCO49), Ensifer numidicus...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rejili M,BenAbderrahim MA,Mars M,Sherrier JD

    更新日期:2020-06-01 00:00:00

  • Expression and purification of Clostridium perfringens beta-toxin glutathione S-transferase fusion protein.

    abstract::The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

    更新日期:1995-08-01 00:00:00

  • Nucleotide sequence and analysis of the gene in Borrelia burgdorferi encoding the immunogenic P39 antigen.

    abstract::The P39 antigen is a specific, highly conserved, and immunogenic protein of Lyme disease spirochetes, Borrelia burgdorferi sensu lato. The nucleotide sequence of the gene encoding this protein was determined and found to be the first of two tandemly arranged open reading frames located on the spirochete's chromosome. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Simpson WJ,Cieplak W,Schrumpf ME,Barbour AG,Schwan TG

    更新日期:1994-06-15 00:00:00

  • Quantification of class 1 integron abundance in natural environments using real-time quantitative PCR.

    abstract::Integrons are bacterial genetic elements capable of capturing and expressing potentially adaptive genetic material. Class 1 integrons constitute the most intensely studied group of these elements to date, mainly due to their well-established role in the acquisition and dissemination of antibiotic resistance genes in c...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Hardwick SA,Stokes HW,Findlay S,Taylor M,Gillings MR

    更新日期:2008-01-01 00:00:00