Biochemical, electrophoretic and immunohistochemical aspects of malate dehydrogenase in truffles (Ascomycotina).

abstract：:The gacA gene of the biocontrol strain Pseudomonas fluorescens CHA0 codes for a response regulator which, together with the sensor kinase GacS (=LemA), is required for the production of exoenzymes and secondary metabolites involved in biocontrol, including hydrogen cyanide (HCN). A gacA multicopy suppressor was isolat...

journal_title：FEMS microbiology letters

authors： Blumer C,Haas D

更新日期：2000-06-01 00:00:00

abstract：:Two Pseudomonas aeruginosa genes encoding the enzymes 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase, both involved in the mevalonate-independent biosynthesis of isoprenoids, have been expressed as recombinant enzymes in Escherichia coli. The purified P. aeruginosa DXP reductoisomerase was inhi...

journal_title：FEMS microbiology letters

authors： Altincicek B,Hintz M,Sanderbrand S,Wiesner J,Beck E,Jomaa H

更新日期：2000-09-15 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa uses quorum-sensing systems to regulate collective behaviour in response to the environment, by linking the expression of particular genes to population density. The quorum-sensing transcription factors LasR and RhlR and their cognate N-acyl-homoserine lactone (HSL) si...

journal_title：FEMS microbiology letters

authors： Miklavič Š,Kogovšek P,Hodnik V,Korošec J,Kladnik A,Anderluh G,Gutierrez-Aguirre I,Maček P,Butala M

更新日期：2015-05-01 00:00:00

abstract：:A composite transposon is a mobile genetic element consisting of two insertion sequences (ISs) flanking a segment of cargo DNA often containing antibiotic resistance (AR) genes. Composite transposons can move as a discreet unit. There have been recently several reports on a novel mechanism of movement of an IS26-based...

journal_title：FEMS microbiology letters

authors： Tansirichaiya S,Mullany P,Roberts AP

更新日期：2016-09-01 00:00:00

abstract：:Chlamydophila pneumoniae infection is associated with a range of diseases including pneumonia, asthma and heart disease. Although an obligate intracellular pathogen, high levels of antigen-specific antibody are induced and serology is frequently used to diagnose these infections. Proteins targeted by the humoral respo...

journal_title：FEMS microbiology letters

authors： Cunningham AF,Ward ME

更新日期：2003-10-10 00:00:00

abstract：:The enterotoxigenic Escherichia coli (ETEC) strain Ec2173, causing post weaning diarrhoea in swine, harbours six plasmids ranging from 13 to 200 kb in size. The heat stable toxin genes sta, stb and a tetracycline resistance gene were located on a self conjugative 120-kb plasmid, called pTC. In the cloned ColE1 type or...

journal_title：FEMS microbiology letters

authors： Olasz F,Fekete PZ,Blum-Oehler G,Boldogkoi Z,Nagy B

更新日期：2005-03-15 00:00:00

abstract：:Two hybridoma cell lines producing monoclonal antibodies (MAbs) against a hemagglutinin/protease (HA/P) from Vibrio cholerae non-01 were produced and characterized. The two MAbs contained the kappa light chain and were IgG1 type. They similarly neutralized HA/P protease activity derived from both V. cholerae non-01 an...

journal_title：FEMS microbiology letters

authors： Honda T,Hata-Naka A,Lertpocasombat K,Miwatani T

更新日期：1991-03-01 00:00:00

abstract：:Human cell lines are often infected by mycoplama strains. We have demonstrated that when infected by Mycoplasma fermentans, human B lymphoma cell proliferation increased strongly. These infected B cells expressed a p45 kDa protein which interacted with the intracellular domain of CD21, the EBV/C3d receptor. p45 analys...

journal_title：FEMS microbiology letters

authors： Balbo M,Barel M,Lottin-Divoux S,Jean D,Frade R

更新日期：2005-08-15 00:00:00

abstract：:The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA ...

journal_title：FEMS microbiology letters

authors： Sharma S,Mittal S,Mallik S,Virdi JS

更新日期：2006-04-01 00:00:00

abstract：:Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

journal_title：FEMS microbiology letters

authors： Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

更新日期：2012-02-01 00:00:00

abstract：:Pythium drechsleri Paul was described as a new species from soil samples taken in a salt-marsh of Arzew, Algeria [Paul, B. (1988) Une nouvelle espèce de Pythium isolée d'une saline de l'ouest Algérien. Cryptogam. Mycol. 9, 325-333]. The name of the fungus, P. drechsleri, is a nomen invalidum, as it is a later homonym ...

journal_title：FEMS microbiology letters

authors： Paul B

更新日期：2000-02-01 00:00:00

abstract：:The application of series of high intensity electric pulses to a yeast suspension provoked a considerable release of some cytoplasmic proteins, glutathione reductase, 3-phosphoglycerate kinase and alcohol dehydrogenase. A maximal yield was achieved 3-8 h after pulsation. The electro-induced protein efflux was accelera...

journal_title：FEMS microbiology letters

authors： Ganeva V,Galutzov B

更新日期：1999-05-15 00:00:00

abstract：:Sequences from the ribosomal DNA ITS regions have shown that there is limited variation among the species in the Trichoderma viride/atroviride/koningii species complex also known as Hypocrea rufa complex, and that infraspecies variation sometimes is larger than interspecies variation. Strains belonging to T. viride, T...

journal_title：FEMS microbiology letters

authors： Lübeck M,Bulat S,Alekhina I,Lieckfeldt E

更新日期：2004-08-15 00:00:00

abstract：:Corynebacterium diphtheriae strains express non-fimbrial surface proteins able to recognize and bind to specific host cells receptors. Protein extracts were obtained from bacterial cells by mechanical process and ammonium sulfate precipitation at 25 and 45% (w/v) saturation. SDS-PAGE analysis of the extracts detected ...

journal_title：FEMS microbiology letters

authors： Colombo AV,Hirata R Jr,de Souza CM,Monteiro-Leal LH,Previato JO,Formiga LC,Andrade AF,Mattos-Guaraldi AL

更新日期：2001-04-13 00:00:00

abstract：:A Ca(2+)/calmodulin-dependent protein kinase (CaMK) gene was cloned and characterized from Arthrobotrys dactyloides, a nematode-trapping fungus. The resulting 373-amino-acid protein, FCaMK, has significant homology to mammalian CaMKs. FCaMK contains a serine/threonine kinase domain followed by a calmodulin-binding dom...

journal_title：FEMS microbiology letters

authors： Tsai PJ,Tu J,Chen TH

更新日期：2002-06-18 00:00:00

abstract：:A Tn5 transposon mutant was isolated of the alkylphenol degrader Pseudomonas sp. KL28 that showed increased growth at higher levels of m-cresol on solid and in liquid cultures. The transposon was inserted at the 5'-terminus of rpoS, which encodes a stationary-phase sigma factor. When grown on agar plates, the rpoS mut...

journal_title：FEMS microbiology letters

authors： Yun JI,Cho KM,Kim JK,Lee SO,Cho K,Lee K

更新日期：2007-04-01 00:00:00

abstract：:The multiple antibiotic resistance operon (marRAB) is a member of the multidrug resistance (mdr) systems. Similar to other mdr systems, this operon when induced encodes resistance to structurally and functionally unrelated antibiotics. marRAB has been shown to be conserved in the family Enterobacteriaceae, but within ...

journal_title：FEMS microbiology letters

authors： Kunonga NI,Sobieski RJ,Crupper SS

更新日期：2000-06-15 00:00:00

abstract：:An identification method by PCR, specific to the Carnobacterium genus, was optimised by testing it on 28 bacterial strains. Primers from the literature were tested to differentiate Carnobacterium strains present among various bacterial species. The DNA of Carnobacterium species (C. alterfunditum, C. divergens, C. fund...

journal_title：FEMS microbiology letters

authors： Cailliez-Grimal C,Miguindou-Mabiala R,Leseine M,Revol-Junelles AM,Millière JB

更新日期：2005-09-01 00:00:00

abstract：:Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucos...

journal_title：FEMS microbiology letters

authors： Takagi H,Awano N,Kobayashi S,Noji M,Saito K,Nakamori S

更新日期：1999-10-15 00:00:00

abstract：:Helicobacter pylori ATCC43504 responds chemotactically to aspartic acid and serine, but not to arginine, nor to sodium bicarbonate. In contrast, H. pylori ATCC700392 (strain 26695) shows chemotaxis to all four attractants. Open reading frame HP0099 from H. pylori 26695 is predicted to encode one of three methyl-accept...

journal_title：FEMS microbiology letters

authors： Cerda O,Rivas A,Toledo H

更新日期：2003-07-29 00:00:00

abstract：:A genomic bank was constructed in Escherichia coli HB101, consisting of DNA fragments from Bacteroides thetaiotaomicron strain 489 inserted within the vector pBR322. By screening on complex medium containing blue dextran, 10 stable dextranase-positive (Dex+) clones were isolated. Seven groups of Dex+ inserts were iden...

journal_title：FEMS microbiology letters

authors： Joncquiert JC,Béchet M,Tierny Y,Courtois J,Dubourguier HC,Guillaume JB

更新日期：1991-12-01 00:00:00

abstract：:Because of an association of human neuroborreliosis with the development of an antibody response against an antigen in neural tissue that cross-reacts with an epitope on the flagellin protein of Borrelia burgdorferi, C3H transgenic mice were created that expressed the flagellin epitope (amino acids 213-224) as a fusio...

journal_title：FEMS microbiology letters

authors： Fikrig E,Barthold SW

更新日期：1997-03-15 00:00:00

abstract：:A strain of Agromyces fucosus, designated Vs2, metabolized a range of organophosphonate compounds as sole phosphorus sources for growth and metabolized phosphonoacetate as a sole carbon, energy and phosphorus source for growth. With phosphonoacetate as the sole phosphorus source and a pyruvate carbon source, transient...

journal_title：FEMS microbiology letters

authors： O'Loughlin SN,Graham RL,McMullan G,Ternan NG

更新日期：2006-08-01 00:00:00

abstract：:This review describes the factors which are currently recognized as being central to the virulence of the human pathogen, Vibrio vulnificus. This estuarine/marine bacterium occurs in high numbers in molluscan shellfish, primarily oysters, and its ingestion in raw oysters results in a ca. 60% mortality in those persons...

journal_title：FEMS microbiology letters

authors： Linkous DA,Oliver JD

更新日期：1999-05-15 00:00:00

abstract：:We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to...

journal_title：FEMS microbiology letters

authors： Devillard E,Newbold CJ,Scott KP,Forano E,Wallace RJ,Jouany JP,Flint HJ

更新日期：1999-12-01 00:00:00

abstract：:The asexual spore, or conidium, is critical in the life cycle of many fungi because it is the primary means for dispersion and serves as a 'safe house' for the fungal genome in adverse environmental conditions. This review discusses the physiological process of germination, conidial adhesion and initiation of protein ...

journal_title：FEMS microbiology letters

authors： Osherov N,May GS

更新日期：2001-05-30 00:00:00

abstract：:The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) ...

journal_title：FEMS microbiology letters

authors： Edwards K,Linetsky I,Hueser C,Eisenstark A

更新日期：2001-05-30 00:00:00

abstract：:Environmental persistence of Mycobacterium tuberculosis is subject to speculation. However, the reality that infected postmortem tissues can be a danger to pathologists and embalmers has worrisome implications. A few experimental studies have demonstrated the organism's ability to withstand exposure to embalming fluid...

journal_title：FEMS microbiology letters

authors： Lemma E,Zimhony O,Greenblatt CL,Koltunov V,Zylber MI,Vernon K,Spigelman M

更新日期：2008-06-01 00:00:00

abstract：:The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

journal_title：FEMS microbiology letters

authors： Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

更新日期：2019-10-01 00:00:00

abstract：:We have constructed a very efficient synthesis and secretion system for cholera toxin B subunit (CTB) of Vibrio cholerae 569B using Bacillus-brevis. The constructed expression-secretion vector has the multiple promoters and the signal peptide coding region of the mwp gene, a structural gene for one of the major cell w...

journal_title：FEMS microbiology letters

authors： Ichikawa Y,Yamagata H,Tochikubo K,Udaka S

更新日期：1993-08-01 00:00:00