PCR-based detection of composite transposons and translocatable units from oral metagenomic DNA.

abstract：:A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide s...

journal_title：FEMS microbiology letters

authors： De Maeseneire SL,De Groeve MR,Dauvrin T,De Mey M,Soetaert W,Vandamme EJ

更新日期：2006-08-01 00:00:00

abstract：:Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in ...

journal_title：FEMS microbiology letters

authors： Bäumchen C,Krings E,Bringer S,Eggeling L,Sahm H

更新日期：2009-01-01 00:00:00

abstract：:Sphingobacterium sp. RB, a novel bacterial strain isolated from a soil sample, was able to utilize para-nitrophenol (PNP) as sole source of carbon and energy at high concentrations (1.0-5.0 mM). The culture completely degraded 3.0 mM PNP within 36 h with proportionate increase in biomass. With 5.0 mM PNP (700 ppm), 70...

journal_title：FEMS microbiology letters

authors： Samson R,Bodade R,Zinjarde S,Kutty R

更新日期：2019-07-01 00:00:00

abstract：:It is known that certain yeast strains, so called 'killers', can produce and excrete proteinaceous toxins that can induce death of other sensitive strains. We obtained a stable fungicidal factor (SKF) through concentration and stabilization of the excretion product of certain killer strains of Saccharomyces cerevisiae...

journal_title：FEMS microbiology letters

authors： Bracesco N,Salvo VA,Carrau FM,Nunes E

更新日期：2006-03-01 00:00:00

abstract：:As shown by both bioassay and high-performance liquid chromatographic (HPLC) analysis, penicillin G production by Aspergillus nidulans is subject to regulation by the pH of the growth medium. Penicillin titres were highest at alkaline pH and in strains carrying mutations in the regulatory gene pacC which mimics the ef...

journal_title：FEMS microbiology letters

authors： Shah AJ,Tilburn J,Adlard MW,Arst HN Jr

更新日期：1991-01-15 00:00:00

abstract：:DNA uptake in competent Streptococcus pneumoniae was strongly dependent on intracellular pH. Ionophore treatments that either acidified or alkalinized the cytoplasm reduced DNA transport. This indicates that the optimum pH for DNA uptake corresponds to the intracellular pH of competent bacteria which is 8.3 +/- 0.2. I...

journal_title：FEMS microbiology letters

authors： Clavé C,Trombe MC

更新日期：1989-11-01 00:00:00

abstract：:We have constructed a very efficient synthesis and secretion system for cholera toxin B subunit (CTB) of Vibrio cholerae 569B using Bacillus-brevis. The constructed expression-secretion vector has the multiple promoters and the signal peptide coding region of the mwp gene, a structural gene for one of the major cell w...

journal_title：FEMS microbiology letters

authors： Ichikawa Y,Yamagata H,Tochikubo K,Udaka S

更新日期：1993-08-01 00:00:00

abstract：:Analysis of the nucleotide sequence downstream from the Xanthomonas oryzae pv. oryzae recA gene reveals two orfs designated orfX and recX. The former has the potential to code for a 5.6 kDa protein of unknown function while the latter encodes for a putative 14.6 kDa protein with homology to RecX from various bacteria....

journal_title：FEMS microbiology letters

authors： Sukchawalit R,Vattanaviboon P,Utamapongchai S,Vaughn G,Mongkolsuk S

更新日期：2001-11-27 00:00:00

abstract：:Probing protein extracts from exponentially growing and stationary phase cultures of Mycobacterium bovis BCG with anti-phospho amino acid antibodies revealed a 31-kDa anti-phospho threonine antibody-reactive protein specific to growing culture. The corresponding protein was purified via two-dimensional gel electrophor...

journal_title：FEMS microbiology letters

authors： Sinha I,Boon C,Dick T

更新日期：2003-10-10 00:00:00

abstract：:Burkholderia mallei, the etiologic agent of the disease known as glanders, is primarily a disease affecting horses and is transmitted to humans by direct contact with infected animals. The use of B. mallei as a biological weapon has been reported and currently, there is no vaccine available for either humans or animal...

journal_title：FEMS microbiology letters

authors： Whitlock GC,Estes DM,Torres AG

更新日期：2007-12-01 00:00:00

abstract：:The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the ...

journal_title：FEMS microbiology letters

authors： Whiting GC,Gillespie SH

更新日期：1996-05-01 00:00:00

abstract：:Mycoplasma mycoides subsp. mycoides (Mmm) strain Afadé had previously been shown to undergo spontaneous phase variations between an opaque capsulated variant and a translucent (TR) variant devoid of a capsule but able to secrete cell-free exopolysaccharides. This phase variation is associated with an ON/OFF genetic sw...

journal_title：FEMS microbiology letters

authors： Gaurivaud P,Lakhdar L,Le Grand D,Poumarat F,Tardy F

更新日期：2014-10-01 00:00:00

abstract：:Marine viruses are the most abundant biological entity in the oceans, the majority of which infect bacteria and are known as bacteriophages. Yet, the bulk of bacteriophages form part of the vast uncultured dark matter of the microbial biosphere. In spite of the paucity of cultured marine bacteriophages, it is known th...

journal_title：FEMS microbiology letters

authors： Perez Sepulveda B,Redgwell T,Rihtman B,Pitt F,Scanlan DJ,Millard A

更新日期：2016-08-01 00:00:00

abstract：:We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. ga...

journal_title：FEMS microbiology letters

authors： Kanda K,Hiramatsu K,Yokota T

更新日期：1990-10-01 00:00:00

abstract：:Hyphae of the human pathogenic fungus Candida albicans exhibit thigmotropic behaviour in vitro, in common with phytopathogenic and saprotrophic fungi. An examination of the literature on C. albicans hyphal penetration of epithelial and endothelial membranes does not support the premise that hyphal thigmotropism plays ...

journal_title：FEMS microbiology letters

authors： Davies JM,Stacey AJ,Gilligan CA

更新日期：1999-02-15 00:00:00

abstract：:In order to clarify the enteropathogenicity of Plesiomonas shigelloides, we investigated a cytotoxin produced by the P-1 strain isolated from patients suffering from diarrhea. The cytotoxicity of the culture filtrate of the strain reached a maximum in culture at 37 degrees C after 12 h shaken in BHI medium. The cytoto...

journal_title：FEMS microbiology letters

authors： Okawa Y,Ohtomo Y,Tsugawa H,Matsuda Y,Kobayashi H,Tsukamoto T

更新日期：2004-10-01 00:00:00

abstract：:The ultrastructure of hepatitis B virus surface antigen (HBsAg) particles produced by recombinant yeast cells was examined using high-resolution negative staining, and ice embedding, electron microscopy. With negative staining, the HBsAg particles were spherical to slightly ovoid with a mean diameter of 27.5 nm and co...

journal_title：FEMS microbiology letters

authors： Yamaguchi M,Sugahara K,Shiosaki K,Mizokami H,Takeo K

更新日期：1998-08-15 00:00:00

abstract：:The small noncoding RNA RyhB is a regulator of iron homeostasis in Escherichia coli. During iron limitation, it downregulates the expression of a number of iron-containing proteins, including enzymes of the tricarboxylic acid cycle and the respiratory chain. Because this infers a potential for RyhB to limit energy met...

journal_title：FEMS microbiology letters

authors： Bollinger CJ,Kallio PT

更新日期：2007-10-01 00:00:00

abstract：:The diheme cytochromes c of the widespread TsdA family are bifunctional thiosulfate dehydrogenase/tetrathionate reductases. Here, biochemical information was collected about TsdA from the Epsilonproteobacterium Wolinella succinogenes (WsTsdA). The situation in W. succinogenes is unique since TsdA is closely associated...

journal_title：FEMS microbiology letters

authors： Kurth JM,Schuster A,Seel W,Herresthal S,Simon J,Dahl C

更新日期：2017-02-01 00:00:00

abstract：:A synthetic version of the ligA gene encoding the NAD-dependent DNA ligase from the Arctic bacterium Pseudoalteromonas haloplanktis was substituted for its homolog in the chromosome of Mycobacterium smegmatis. The resulting recombinant strain grew identically to the parent strain at permissive temperatures but failed ...

journal_title：FEMS microbiology letters

authors： Pinto CT,Nano FE

更新日期：2015-09-01 00:00:00

abstract：:Marine snow aggregates are microbial hotspots that support high bacterial abundance and activities. We conducted laboratory experiments to compare cell-specific bacterial protein production (BPP) and protease activity between free-living and attached bacteria. Natural bacterial assemblages attached to model aggregates...

journal_title：FEMS microbiology letters

authors： Grossart HP,Tang KW,Kiørboe T,Ploug H

更新日期：2007-01-01 00:00:00

abstract：:Dextranase specifically hydrolyzes dextran and is used to produce functional isomalto-saccharide prebiotics. Moreover, dextranase is used as an additive in mouthwash to remove dental plaque. We cloned and expressed the dextranase gene of the marine bacterium Bacillus aquimaris S5. The length of the BaDex gene was 1788...

journal_title：FEMS microbiology letters

authors： Dong D,Wang X,Deng T,Ning Z,Tian X,Zu H,Ding Y,Wang C,Wang S,Lyu M

更新日期：2021-01-20 00:00:00

abstract：:The viability of probiotics is affected by several factors during manufacturing, storage and gastrointestinal tract passage. Protecting the probiotics from harmful conditions is particularly critical for oxygen sensitive species like Akkermansia muciniphila, a bacterium which recently has been proposed as a next-gener...

journal_title：FEMS microbiology letters

authors： Marcial-Coba MS,Saaby L,Knøchel S,Nielsen DS

更新日期：2019-01-01 00:00:00

abstract：:Molecular microbial ecology studies are heavily reliant on 'Universal' 16S rRNA gene primers for elucidating microbial community structure and composition, and yet primer design and optimization is often overlooked. Primers that exhibit minor biases due to primer-template mismatches can substantially alter the pool of...

journal_title：FEMS microbiology letters

authors： Thomas MC,Thomas DK,Selinger LB,Inglis GD

更新日期：2011-07-01 00:00:00

abstract：:Twelve strains of Candida albicans were grown in defined medium which had been deferrated by ion-exchange chromatography and then supplemented with FeCl3 to give iron concentrations ranging from 0.026 microM (growth-limiting) to 0.8 microM (excess). All of the strains secreted hydroxamate-type siderophores; phenolate ...

journal_title：FEMS microbiology letters

authors： Sweet SP,Douglas LJ

更新日期：1991-05-01 00:00:00

abstract：:Acinetobacter baumannii strain A148, a clinical isolate resistant to imipenem (MIC = 32 mg l-1), synthesized two beta-lactamases with pIs 6.3 and > 9.2. The pI 6.3 enzyme hydrolyzed the penicillins, including isoxazoylpenicillins, first-, second- and, to a lesser extent, third-generation cephalosporins. It was inhibit...

journal_title：FEMS microbiology letters

authors： Hornstein M,Sautjeau-Rostoker C,Péduzzi J,Vessières A,Hong LT,Barthélémy M,Scavizzi M,Labia R

更新日期：1997-08-15 00:00:00

abstract：:Burkholderia pseudomallei, a Gram-negative bacterium is found in the soil and water, mainly in Southeast Asia and Northern Australia. It is responsible for melioidosis in human and animals. The bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. The is...

journal_title：FEMS microbiology letters

authors： Lee MA,Liu Y

更新日期：2000-11-01 00:00:00

abstract：:We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were firs...

journal_title：FEMS microbiology letters

authors： Liu C,Song Y,McTeague M,Vu AW,Wexler H,Finegold SM

更新日期：2003-05-16 00:00:00

abstract：:Volvariella volvacea, strain V14, produces multiple forms of extracellular laccase when grown in submerged culture in a defined medium with glucose as sole carbon source, and on cotton waste 'compost' representative of the conditions used for industrial-scale mushroom cultivation. In liquid culture, enzyme synthesis i...

journal_title：FEMS microbiology letters

authors： Chen S,Ma D,Ge W,Buswell JA

更新日期：2003-01-21 00:00:00

abstract：:Lactobacillus johnsonii La1 (La1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. This study evaluates how the ingestion of different amounts of La1 influences the main bacterial populations of the fecal microbiota. Eight asymptomatic voluntee...

journal_title：FEMS microbiology letters

authors： Garrido D,Suau A,Pochart P,Cruchet S,Gotteland M

更新日期：2005-07-15 00:00:00