A novel dextranase gene from the marine bacterium Bacillus aquimaris S5 and its expression and characteristics.

abstract：:In several bacterial species that show natural transformation, dprA has been described as a competence gene. The DprA protein has been suggested to be involved in the protection of incoming DNA. However, members of the dprA gene family (also called smf) can be detected in virtually all bacterial species, which suggest...

journal_title：FEMS microbiology letters

authors： Smeets LC,Becker SC,Barcak GJ,Vandenbroucke-Grauls CM,Bitter W,Goosen N

更新日期：2006-10-01 00:00:00

abstract：:Bacteriophage M102 is a virulent siphophage that propagates in some serotype c Streptococcus mutans strains, but not in S. mutans of serotype e, f or k. The serotype of S. mutans is determined by the glucose side chain of rhamnose-glucose polysaccharide (RGP). Because the first step in the bacteriophage infection proc...

journal_title：FEMS microbiology letters

authors： Shibata Y,Yamashita Y,van der Ploeg JR

更新日期：2009-05-01 00:00:00

abstract：:Streptomyces tendae Tü901 produces nikkomycins belonging to the nucleoside peptide antibiotics. Mutants defective in histidine catabolism were isolated and characterized with regard to their histidine ammonium-lyase activity and antibiotic synthesis. In the histidine ammonialyase-negative mutant hut-11 which was unimp...

journal_title：FEMS microbiology letters

authors： Roos U,Mattern S,Schrempf H,Bormann C

更新日期：1992-10-01 00:00:00

abstract：:Lactic acid bacteria were screened for methional production from 4-methylthio-2-ketobutanoate. Only Lactococcus lactis IFPL730 produced high amounts of methional. It was demonstrated that production of this compound was an exclusively enzymatic reaction. The present work describes for the first time that L. lactis can...

journal_title：FEMS microbiology letters

authors： Amárita F,Fernández-Esplá D,Requena T,Pelaez C

更新日期：2001-10-16 00:00:00

abstract：:Comparison of sequence data is necessary in older to investigate virus origins, identify features common to virulent strains, and characterize genomic organization within virus families. A virulent caliciviral disease of rabbits recently emerged in China. We have sequenced 1100 bases from the 3' ends of two independen...

journal_title：FEMS microbiology letters

authors： Milton ID,Vlasak R,Nowotny N,Rodak L,Carter MJ

更新日期：1992-05-15 00:00:00

abstract：:We report the draft genome sequence from Aeromonas salmonicida sp. strain CBA100, which was characterized as an antibiotic-resistant bacterium isolated from infected rainbow trout. The total size of the genome is 4,788,109 bp, with a G + C content of 60.55%. Comparison of its open reading frames shows that the closest...

journal_title：FEMS microbiology letters

authors： Valdes N,Espinoza C,Sanhueza L,Gonzalez A,Corsini G,Tello M

更新日期：2015-03-01 00:00:00

abstract：:The distribution of the alcohol dehydrogenase gene (adh) among different Archaea was investigated by Southern blot analysis revealing the potentiality of the adh gene as a specific marker for the genus Sulfolobus. Moreover, the in vivo expression of the adh gene from a new isolate of Sulfolobus solfataricus, G theta, ...

journal_title：FEMS microbiology letters

authors： Cannio R,Fiorentino G,Rossi M,Bartolucci S

更新日期：1999-01-01 00:00:00

abstract：:Two bifunctional plasmid vectors pZG5 (7.45 kb) and pZG6 (6.95 kb), for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of the multicopy broad-host-range Streptomyces plasmid pIJ350 with E. coli plasmids Bluescribe M13- (pZG5) or pUC18 (pZG6). Both plasmids possess several ...

journal_title：FEMS microbiology letters

authors： Durajlija S,Pigac J,Gamulin V

更新日期：1991-10-15 00:00:00

abstract：:In Salmonella typhimurium the metE and metR promoters overlap and are divergently transcribed. Three tandem repeats of an 8 bp sequence defined previously as the metE operator site for MetJ-mediated repression also overlap the -35 region of the metR promoter. Starting with a metE-lacZ.metR-galK double gene fusion, sit...

journal_title：FEMS microbiology letters

authors： Wu WF,Urbanowski ML,Stauffer GV

更新日期：1993-04-01 00:00:00

abstract：:A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide s...

journal_title：FEMS microbiology letters

authors： De Maeseneire SL,De Groeve MR,Dauvrin T,De Mey M,Soetaert W,Vandamme EJ

更新日期：2006-08-01 00:00:00

abstract：:mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These st...

journal_title：FEMS microbiology letters

authors： Saito T,Okano K,Park HD,Itayama T,Inamori Y,Neilan BA,Burns BP,Sugiura N

更新日期：2003-12-12 00:00:00

abstract：:Herbaspirillum seropedicae strains mutated in the nifX or orf1 genes showed 90% or 50% reduction in nitrogenase activity under low levels of iron or molybdenum respectively. Mutations in nifX or orf1 genes did not affect nif gene expression since a nifH::lacZ fusion was fully active in both mutants. nifX and the conti...

journal_title：FEMS microbiology letters

authors： Klassen G,de Oliveira Pedrosa F,de Souza EM,Yates MG,Rigo LU

更新日期：2003-07-29 00:00:00

abstract：:A Gram-negative, facultatively anaerobic, motile and slightly curved rod-shaped bacterium (BFLP-4(T)) was isolated from the faeces of wild seahorses (Hippocampus guttulatus) captured in northwest Spain (Toralla, Galicia). Strain BFLP-4(T) grew at 10-35 degrees C and pH 5-9 (optimally at 20 degrees C and pH 7.2) and at...

journal_title：FEMS microbiology letters

authors： Balcázar JL,Pintado J,Planas M

更新日期：2010-06-01 00:00:00

abstract：:A lysC-lac'Z fusion plasmid was constructed to study the regulatory region of the lysC gene. Analysis by deletion mutations confirmed the existence of an alternative promoter, P2, located upstream of the previously identified promoter, P1. The transcription start site of promoter P2 was located 85 base pairs upstream ...

journal_title：FEMS microbiology letters

authors： Liao HH,Hseu TH

更新日期：1998-11-01 00:00:00

abstract：:The ultrastructure of hepatitis B virus surface antigen (HBsAg) particles produced by recombinant yeast cells was examined using high-resolution negative staining, and ice embedding, electron microscopy. With negative staining, the HBsAg particles were spherical to slightly ovoid with a mean diameter of 27.5 nm and co...

journal_title：FEMS microbiology letters

authors： Yamaguchi M,Sugahara K,Shiosaki K,Mizokami H,Takeo K

更新日期：1998-08-15 00:00:00

abstract：:An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively. The enzyme was stable up to 1 h at 50 degrees C a...

journal_title：FEMS microbiology letters

authors： Zanoelo FF,Polizeli Mde L,Terenzi HF,Jorge JA

更新日期：2004-11-15 00:00:00

abstract：:Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucos...

journal_title：FEMS microbiology letters

authors： Takagi H,Awano N,Kobayashi S,Noji M,Saito K,Nakamori S

更新日期：1999-10-15 00:00:00

abstract：:Francisella tularensis is a highly virulent bacterial species that causes various forms of tularemia in humans. The urgency in understanding the pathogenesis of these diseases has stimulated unprecedented interest in this bacterial species over the past few years. Recent findings underscore a number of important disti...

journal_title：FEMS microbiology letters

authors： Parmely MJ,Fischer JL,Pinson DM

更新日期：2009-11-01 00:00:00

abstract：:The synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB+ strain. Immunoblotting expe...

journal_title：FEMS microbiology letters

authors： Sumantran VN,Tranguch AJ,Datta P

更新日期：1989-11-01 00:00:00

abstract：:In order to find reasons for the absence of fire blight in most countries of the Southern hemisphere, bark samples from apple and pear trees in orchards of the Western Cape region in South Africa were extracted for bacteria which could be antagonistic to Erwinia amylovora. Screening was done in the late growth season ...

journal_title：FEMS microbiology letters

authors： Jock S,Völksch B,Mansvelt L,Geider K

更新日期：2002-06-04 00:00:00

abstract：:The phylogenetic position of an acidophilic chemo-organotrophic menaquinone-containing bacterium, Acidobacterium capsulatum, was studied on the basis of 16S rRNA gene sequence information. A. capsulatum showed the highest level of sequence similarity to Heliobacterium chlorum, a member of the Gram-positive group, yet ...

journal_title：FEMS microbiology letters

authors： Hiraishi A,Kishimoto N,Kosako Y,Wakao N,Tano T

更新日期：1995-10-01 00:00:00

abstract：:This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong ind...

journal_title：FEMS microbiology letters

authors： Sadaie Y,Nakadate H,Fukui R,Yee LM,Asai K

更新日期：2008-02-01 00:00:00

abstract：:Atypical enteropathogenic Escherichia coli (EPEC) comprise an important group of paediatric pathogens. Atypical EPEC have reservoirs in farm and domestic animals where they can be either commensal or pathogenic; serogroup O26 is dominant in humans and animals. Central to intestinal colonization by EPEC is the transloc...

journal_title：FEMS microbiology letters

authors： Ooka T,Vieira MA,Ogura Y,Beutin L,La Ragione R,van Diemen PM,Stevens MP,Aktan I,Cawthraw S,Best A,Hernandes RT,Krause G,Gomes TA,Hayashi T,Frankel G

更新日期：2007-06-01 00:00:00

abstract：:Using labelled, gamma-32P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by agarose gel electro...

journal_title：FEMS microbiology letters

authors： Kanaujia GV,Katoch VM,Shivannavar CT,Sharma VD,Patil MA

更新日期：1991-01-15 00:00:00

abstract：:Stenotrophomonas maltophilia is a multidrug-resistant opportunistic pathogen. S. maltophilia quorum-sensing system is mediated by the diffusible signal factor (DSF), which synthesis depends on rpfF. It has been reported that rpfF disruption in S. maltophilia K279a leads to a loss of DSF synthesis, reduced levels of ex...

journal_title：FEMS microbiology letters

authors： Alcaraz E,García C,Friedman L,de Rossi BP

更新日期：2019-03-01 00:00:00

abstract：:The nitrogen-fixing cyanobacterium Nostoc sp. ATCC 29132 was shown to contain two sequence-specific endonucleases. Nsp(29132) I was an isoschizomer of AsuII, and Nsp(29132) II was an isoschizomer of BamHI. Nsp(29132) II was shown to generate ends that could be ligated to those generated by BamHI. ...

journal_title：FEMS microbiology letters

authors： Muro-Pastor AM,Herrero A,Flores E

更新日期：1991-01-01 00:00:00

abstract：:Mesophilic Aeromonas hydrophila from serotypes O:11 and O:34 grown in a glucose-rich medium produce a capsule that can be seen under light and electron microscopy. The purified capsular polysaccharide has a composition qualitatively similar for strains O:11 and O:34, but quantitatively different. The capsular polysacc...

journal_title：FEMS microbiology letters

authors： Martínez MJ,Simon-Pujol D,Congregado F,Merino S,Rubires X,Tomás JM

更新日期：1995-04-15 00:00:00

abstract：:The type III secretion system (T3SS) is a specialized apparatus evolved by Gram-negative bacteria to deliver effector proteins into host cells, thus facilitating the establishment of an infection. Effector translocation across the target cell plasma membrane is believed to occur via pores formed by at least two secret...

journal_title：FEMS microbiology letters

authors： Bröms JE,Edqvist PJ,Forsberg A,Francis MS

更新日期：2006-03-01 00:00:00

abstract：:Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters (prfAP1, prfAP2, and PplcA). The prfAP2 promoter was previously proposed to be sigma B (sigma(B))-dependent. This hypothesis was tested by creating prfA promoter-gus transcriptional fusions in both L. monocytogenes ...

journal_title：FEMS microbiology letters

authors： Schwab U,Bowen B,Nadon C,Wiedmann M,Boor KJ

更新日期：2005-04-15 00:00:00

abstract：:The Mycobacterium tuberculosis murG gene, Rv2153, was expressed in Escherichia coli murG(Ts) strain OV58 on a plasmid under the control of the arabinose-inducible araBAD promoter. Mycobacterium tuberculosis murG rescued the growth of E. coli murG(Ts) at the nonpermissive temperature: transformants were only obtained i...

journal_title：FEMS microbiology letters

authors： Jha RK,Katagihallimath N,Hota SK,Das KS,de Sousa SM

更新日期：2012-01-01 00:00:00