Incorporation of choline into Streptococcus pneumoniae cell wall antigens: evidence for choline kinase activity.

abstract：:The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

journal_title：FEMS microbiology letters

authors： Pissavin C,Hugouvieux-Cotte-Pattat N

更新日期：1997-12-01 00:00:00

abstract：:In haloarchaea, a cluster of three genes is localized directly adjacent to the major replication origin, and, hence, the encoded proteins were annotated as 'origin-associated proteins' (Oap). However, prior to this study, no experimental data were available for these conserved hypothetical proteins. Bioinformatic anal...

journal_title：FEMS microbiology letters

authors： Wolters M,Borst A,Pfeiffer F,Soppa J

更新日期：2019-11-01 00:00:00

abstract：:The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 microM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity o...

journal_title：FEMS microbiology letters

authors： Song Y,Logan BE

更新日期：2004-10-15 00:00:00

abstract：:In order to investigate whether the iron acquisition mechanisms of Staphylococcus aureus are induced by iron restriction in vitro, we examined S. aureus ATCC 6538 for production of siderophore and expression of transferrin-binding protein (SA-tbp) in normal or deferrated brain heart infusion broth (BHI). Siderophore p...

journal_title：FEMS microbiology letters

authors： Lim Y,Shin SH,Lee SI,Kim IS,Rhee JH

更新日期：1998-06-01 00:00:00

abstract：:Mycobacterium leprae incorporated exogenously supplied pyrimidines as bases or nucleosides, but not as a nucleotide, into its nucleic acids. Notably, thymine was incorporated approximately 5 times more rapidly than thymidine by both suspensions of, or intracellular M. leprae. Thymine incorporation was significantly in...

journal_title：FEMS microbiology letters

authors： Wheeler PR

更新日期：1989-01-15 00:00:00

abstract：:Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (...

journal_title：FEMS microbiology letters

authors： Liu X,Feng Z,Harris NB,Cirillo JD,Bercovier H,Barletta RG

更新日期：2001-08-21 00:00:00

abstract：:An enzyme linked immune sorbent assay (ELISA) was developed to measure bacterial surface lipoteichoic acid (LTA). Numerous strains of oral streptococci belonging to the 'viridans' group were examined on three separate occasions. The results show that, under these cultural conditions, oral streptococci do not normally ...

journal_title：FEMS microbiology letters

authors： Hogg SD,Manning JE

更新日期：1989-04-01 00:00:00

abstract：:The precise molecular mechanism of Staphylococcus aureus beta-toxin inactivation by the serotype F triple-converting phage phi 42, phi A1 and phi A3 was investigated. Sequence analysis of the phi 42 (attP) and Staphylococcus aureus (attB) attachment sites and the left (attL) and right (attR) chromosomal/bacteriophage ...

journal_title：FEMS microbiology letters

authors： Carroll JD,Cafferkey MT,Coleman DC

更新日期：1993-01-15 00:00:00

abstract：:Endopolygalacturonases (endoPGs) of some phytopathogens are virulent factors for dicots. To investigate the function of the endoPG of Magnaporthe oryzae, a disruption mutant of MGG_08938, the homolog of endoPG found in the genome database of this fungus, was generated. The pathogenicity, mycelial growth, and appressor...

journal_title：FEMS microbiology letters

authors： Mori T,Jung HY,Maejima K,Hirata H,Himeno M,Hamamoto H,Namba S

更新日期：2008-03-01 00:00:00

abstract：:As shown by both bioassay and high-performance liquid chromatographic (HPLC) analysis, penicillin G production by Aspergillus nidulans is subject to regulation by the pH of the growth medium. Penicillin titres were highest at alkaline pH and in strains carrying mutations in the regulatory gene pacC which mimics the ef...

journal_title：FEMS microbiology letters

authors： Shah AJ,Tilburn J,Adlard MW,Arst HN Jr

更新日期：1991-01-15 00:00:00

abstract：:We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pair...

journal_title：FEMS microbiology letters

authors： Lightbody KL,Renshaw PS,Collins ML,Wright RL,Hunt DM,Gordon SV,Hewinson RG,Buxton RS,Williamson RA,Carr MD

更新日期：2004-09-01 00:00:00

abstract：:An Acremonium chrysogenum strain improvement program based on the transformation with cephalosporin biosynthetic genes was carried out to enhance cephalosporin C production. Best results were obtained with cefEF and cefG genes, selecting transformants with increased cephalosporin C production and lower accumulation of...

journal_title：FEMS microbiology letters

authors： Rodríguez-Sáiz M,Lembo M,Bertetti L,Muraca R,Velasco J,Malcangi A,de la Fuente JL,Barredo JL

更新日期：2004-06-01 00:00:00

abstract：:A family of 2,3-di-O-acyl trehaloses (DAT), previously identified in Mycobacterium fortuitum, was studied by fast-atom bombardment mass spectrometry to establish the combinations of fatty acyl substituents and, hence, to delineate the molecular species there comprised. The mass spectra indicated the possible existence...

journal_title：FEMS microbiology letters

authors： Ariza MA,Valero-Guillén PL

更新日期：1994-06-15 00:00:00

abstract：:The enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. Most strains able to bind amylase exhibited functional enzyme on their ...

journal_title：FEMS microbiology letters

authors： Douglas CW,Heath J,Gwynn JP

更新日期：1992-04-15 00:00:00

abstract：:The genes encoding the desulforubidin type of dissimilatory sulfite reductase (Dsr) from the sulfate-reducing bacteria Desulfobacter vibrioformis and Desulfobulbus rhabdoformis were cloned and sequenced. Similar to the genes for dissimilatory sulfite reductase from the genera Archaeoglobus, Desulfovibrio and Desulfoto...

journal_title：FEMS microbiology letters

authors： Larsen,Lien T,Birkeland NK

更新日期：2000-05-01 00:00:00

abstract：:Corynebacterium diphtheriae, generally considered an extracellular coloniser, was evaluated for its ability to enter and survive within HEp-2 monolayers by gentamicin protection assay. Intracellular viability of HC01 strain, isolated from endocarditis, was more expressive (2.59%) than observed in 241 (0.21%) and CDC-E...

journal_title：FEMS microbiology letters

authors： Hirata R,Napoleão F,Monteiro-Leal LH,Andrade AF,Nagao PE,Formiga LC,Fonseca LS,Mattos-Guaraldi AL

更新日期：2002-09-24 00:00:00

abstract：:The mosquitocidal crystal protein, Cry19Aa, from Bacillus thuringiensis ssp. jegathesan, has high toxicity to Anopheles stephensi and Culex pipiens but is less toxic to Aedes aegypti. To study the functional role of putative domain II and surface residues in mosquito toxicity, 16 alanine substitution mutations were in...

journal_title：FEMS microbiology letters

authors： Roh JY,Nair MS,Liu XS,Dean DH

更新日期：2009-06-01 00:00:00

abstract：:The aims of our research were to investigate the gene expression of the multidrug efflux transporter, CDR1 and the major drug facilitator superfamily transporter, MDR1 gene in azole drug-resistant Candida albicans and Candida glabrata clinical isolates recovered from vaginitis patients; and to identify hotspot mutatio...

journal_title：FEMS microbiology letters

authors： Looi CY,D' Silva EC,Seow HF,Rosli R,Ng KP,Chong PP

更新日期：2005-08-15 00:00:00

abstract：:The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) ...

journal_title：FEMS microbiology letters

authors： Edwards K,Linetsky I,Hueser C,Eisenstark A

更新日期：2001-05-30 00:00:00

abstract：:A synthetic version of the ligA gene encoding the NAD-dependent DNA ligase from the Arctic bacterium Pseudoalteromonas haloplanktis was substituted for its homolog in the chromosome of Mycobacterium smegmatis. The resulting recombinant strain grew identically to the parent strain at permissive temperatures but failed ...

journal_title：FEMS microbiology letters

authors： Pinto CT,Nano FE

更新日期：2015-09-01 00:00:00

abstract：:Brettanomyces/Dekkera yeasts have been identified as part of the grape yeast flora. They are well known for colonizing the cellar environmental and spoiling wines, causing haze, turbidity and strong off-flavours in wines and enhancing the volatile acidity. As the general practices applied to combat Brettanomyces/Dekke...

journal_title：FEMS microbiology letters

authors： Comitini F,Ciani M

更新日期：2011-03-01 00:00:00

abstract：:A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 x 10(4) cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with...

journal_title：FEMS microbiology letters

authors： You X,Arrowood MJ,Lejkowski M,Xie L,Schinazi RF,Mead JR

更新日期：1996-03-01 00:00:00

abstract：:The physiological role of mitochondrial aldehyde dehydrogenase (ALD5) was investigated by analysis of the ald5 mutant (AKD321) in Saccharomyces cerevisiae. K(+)-activated ALDH activity of the ald5 mutant was about 80% of the wild-type in the mitochondrial fraction, while the respiratory activity of the ald5 mutant was...

journal_title：FEMS microbiology letters

authors： Kurita O,Nishida Y

更新日期：1999-12-15 00:00:00

abstract：:Two bifunctional plasmid vectors pZG5 (7.45 kb) and pZG6 (6.95 kb), for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of the multicopy broad-host-range Streptomyces plasmid pIJ350 with E. coli plasmids Bluescribe M13- (pZG5) or pUC18 (pZG6). Both plasmids possess several ...

journal_title：FEMS microbiology letters

authors： Durajlija S,Pigac J,Gamulin V

更新日期：1991-10-15 00:00:00

abstract：:It has been widely reported that pH mediates cadmium toxicity to bacteria. We used a tripartite approach to investigate mechanisms by which pH affects cadmium toxicity that included analyses of: (1) growth kinetics, (2) global gene expression, and (3) cadmium speciation. Cadmium extended the lag phase at pH 7, but not...

journal_title：FEMS microbiology letters

authors： Worden CR,Kovac WK,Dorn LA,Sandrin TR

更新日期：2009-04-01 00:00:00

abstract：:Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (r...

journal_title：FEMS microbiology letters

authors： Skamrov A,Feoktistova E,Goldman M,Beabealashvilli R

更新日期：2002-03-05 00:00:00

abstract：:CheY, the response regulator of the chemotaxis system in Escherichia coli, can be regulated by two covalent modifications - phosphorylation and acetylation. Both covalent modifications are involved in chemotaxis, but the mechanism and role of the acetylation are still obscure. While acetylation was shown to repress th...

journal_title：FEMS microbiology letters

authors： Li R,Chen P,Gu J,Deng JY

更新日期：2013-10-01 00:00:00

abstract：:Penicillium expansum is the causal agent of blue mold rot, a postharvest decay of stored fruits. This fungus also produces the mycotoxins patulin and citrinin. Control of P. expansum still relies mainly on the use of fungicides such as thiabendazole. Since its introduction, resistant strains have been reported. The ai...

journal_title：FEMS microbiology letters

authors： Cabañas R,Castellá G,Abarca ML,Bragulat MR,Cabañes FJ

更新日期：2009-08-01 00:00:00

abstract：:This study provides a novel qRT-PCR protocol for specific detection and proof of viability of Phytophthora in environmental samples based on differential accumulation of cox II transcripts. Chemical and physical treatments were tested for their ability to induce in vitro the accumulation of cytochrome oxidase genes en...

journal_title：FEMS microbiology letters

authors： Vannini A,Tomassini A,Bruni N,Vettraino AM

更新日期：2014-04-01 00:00:00

abstract：:In several bacterial species that show natural transformation, dprA has been described as a competence gene. The DprA protein has been suggested to be involved in the protection of incoming DNA. However, members of the dprA gene family (also called smf) can be detected in virtually all bacterial species, which suggest...

journal_title：FEMS microbiology letters

authors： Smeets LC,Becker SC,Barcak GJ,Vandenbroucke-Grauls CM,Bitter W,Goosen N

更新日期：2006-10-01 00:00:00