Incorporation of choline into Streptococcus pneumoniae cell wall antigens: evidence for choline kinase activity.


:The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the fall in ATP concentration due to phosphorylation of choline. Genomic DNA of S. pneumoniae hybridised with a probe consisting of an internal fragment of the choline kinase gene of Saccharomyces cerevisiae and one consisting of the choline binding domain of lytA.


FEMS Microbiol Lett


Whiting GC,Gillespie SH




Has Abstract


1996-05-01 00:00:00














  • XaxAB-like binary toxin from Photorhabdus luminescens exhibits both insecticidal activity and cytotoxicity.

    abstract::The enterobacterium Photorhabdus luminescens produces a number of toxins to kill its insect host. By analyzing the genomic sequence of P. luminescens TT01, we found that amino acid sequences encoded by plu1961 and plu1962 showed high similarity to XaxAB binary toxin of Xenorhabuds nematophila, which has both necrotic ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zhang X,Hu X,Li Y,Ding X,Yang Q,Sun Y,Yu Z,Xia L,Hu S

    更新日期:2014-01-01 00:00:00

  • Internal size variations in Tn1546-like elements due to the presence of IS1216V.

    abstract::In this study, internal size variations in the VanA gene cluster Tn1546, encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn1546, revealed that these variations were due to the presence of the IS sequenc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jensen LB

    更新日期:1998-12-15 00:00:00

  • Strain improvement for cephalosporin production by Acremonium chrysogenum using geneticin as a suitable transformation marker.

    abstract::An Acremonium chrysogenum strain improvement program based on the transformation with cephalosporin biosynthetic genes was carried out to enhance cephalosporin C production. Best results were obtained with cefEF and cefG genes, selecting transformants with increased cephalosporin C production and lower accumulation of...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rodríguez-Sáiz M,Lembo M,Bertetti L,Muraca R,Velasco J,Malcangi A,de la Fuente JL,Barredo JL

    更新日期:2004-06-01 00:00:00

  • Identification of genes transcribed by Pasteurella multocida in rabbit livers through the selective capture of transcribed sequences.

    abstract::Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera in poultry, hemorrhagic septicemia in cattle, atropic rhinitis in swine, and snuffles in rabbits. The differentially expressed gene profile of P. multocida in infected rabbit livers was identified and compared with t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Guo D,Lu Y,Zhang A,Liu J,Yuan D,Jiang Q,Lin H,Si C,Qu L

    更新日期:2012-06-01 00:00:00

  • Alteration in the composition of cholesteryl glucosides and other lipids in Helicobacter pylori undergoing morphological change from spiral to coccoid form.

    abstract::In this analysis of membrane lipid compositions in Helicobacter pylori, the membrane lipid profiles drastically changed during coccoid formation: cholesteryl-6-O-tetradecanoyl-alpha-D-glucopyranoside levels increased, cholesteryl-alpha-D-glucopyranoside and phosphatidyl ethanolamine decreased, and a coordinated increa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shimomura H,Hayashi S,Yokota K,Oguma K,Hirai Y

    更新日期:2004-08-15 00:00:00

  • Diatom DNA barcodes for forensic discrimination of drowning incidents.

    abstract::The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.I...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Liu M,Zhao Y,Sun Y,Wu P,Zhou S,Ren L

    更新日期:2020-09-01 00:00:00

  • Extension of resistance to cefepime and cefpirome associated to a six amino acid deletion in the H-10 helix of the cephalosporinase of an Enterobacter cloacae clinical isolate.

    abstract::Enterobacter cloacae CHE, a clinical strain with overproduced cephalosporinase was found to be highly resistant to the new cephalosporins, cefepime and cefpirome (MICs> or =128 microg ml(-1)). The strain was isolated from a child previously treated with cefepime. The catalytic efficiency of the purified enzyme with th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Barnaud G,Labia R,Raskine L,Sanson-Le Pors MJ,Philippon A,Arlet G

    更新日期:2001-02-20 00:00:00

  • Occurrence of a lipase in spores of Alternaria brassicicola with a crucial role in the infection of cauliflower leaves.

    abstract::Alternaria brassicicola is a pathogen that penetrates directly through the host cuticle thanks to several serine esterases, according to our findings. Among these, an 80-kDa lipase (E.C 3.1.1. 3) was detected by SDS-PAGE and immunoblotting in the water washings of ungerminated spores. The purified lipase cross-reacted...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Berto P,Comménil P,Belingheri L,Dehorter B

    更新日期:1999-11-15 00:00:00

  • Molecular characterization of benzimidazole-resistant isolates of Cladosporium fulvum.

    abstract::The benzimidazole fungicide thiophanate-methyl is commonly applied to control leaf mould of tomato caused by Cladosporium fulvum in China. In this study, 32 isolates of C. fulvum were examined for their sensitivities to thiophanate-methyl, and two benzimidazole-resistant (BenR) phenotypes BenR1 and BenR2 were identifi...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Yan L,Chen J,Zhang C,Ma Z

    更新日期:2008-01-01 00:00:00

  • Pili of Pasteurella multocida of porcine origin.

    abstract::Using electron microscopy, pili with at least two distinct morphologies were observed on strains of Pasteurella multocida isolated from pigs with atrophic rhinitis. Rigid pili were found on 60-80% of all cells observed. These pili had a strong tendency to lie flat along the side of the outer cell membrane of P. multoc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Isaacson RE,Trigo E

    更新日期:1995-10-15 00:00:00

  • Arthrospira ('Spirulina') strains from four continents are resolved into only two clusters, based on amplified ribosomal DNA restriction analysis of the internally transcribed spacer.

    abstract::We present the results of a phylogenetic study, based on amplified ribosomal DNA restriction analysis of the rDNA operon, of 37 Arthrospira ('Spirulina') cultivated clonal strains from four continents. In addition, duplicates from different culture collections or markedly different morphotypes of particular strains es...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Scheldeman P,Baurain D,Bouhy R,Scott M,Mühling M,Whitton BA,Belay A,Wilmotte A

    更新日期:1999-03-15 00:00:00

  • Antibiotic treatment disrupts bacterial communities in the colon and rectum of simian immunodeficiency virus-infected macaques.

    abstract::Antibiotic therapies are known to disrupt gastrointestinal (GI) bacterial communities. HIV and pathogenic simian immunodeficiency virus (SIV) infections have also been associated with disrupted GI bacterial communities. We administered a combination antibiotic therapy to six SIV-infected rhesus macaques and collected ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zevin AS,Hensley-McBain T,Miller C,Smith E,Langevin S,Klatt NR

    更新日期:2017-12-15 00:00:00

  • Not all animals need a microbiome.

    abstract::It is often taken for granted that all animals host and depend upon a microbiome, yet this has only been shown for a small proportion of species. We propose that animals span a continuum of reliance on microbial symbionts. At one end are the famously symbiont-dependent species such as aphids, humans, corals and cows, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Hammer TJ,Sanders JG,Fierer N

    更新日期:2019-05-01 00:00:00

  • Screening for basidiomycetous fungi capable of degrading 2,7-dichlorodibenzo-p-dioxin.

    abstract::We devised a screening method to obtain basidiomycetous fungi capable of degrading dioxins. About 200 fungal strains were selected from more than 1500 strains by their ability to decolorize Remazol brilliant blue R dye as an indicator. To attempt to eliminate the factor of dioxin sorption by mycelia, we prepared two s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sato A,Watanabe T,Watanabe Y,Harazono K,Fukatsu T

    更新日期:2002-08-06 00:00:00

  • Mutation of rpoS enhances Pseudomonas sp. KL28 growth at higher concentrations of m-cresol and changes its surface-related phenotypes.

    abstract::A Tn5 transposon mutant was isolated of the alkylphenol degrader Pseudomonas sp. KL28 that showed increased growth at higher levels of m-cresol on solid and in liquid cultures. The transposon was inserted at the 5'-terminus of rpoS, which encodes a stationary-phase sigma factor. When grown on agar plates, the rpoS mut...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yun JI,Cho KM,Kim JK,Lee SO,Cho K,Lee K

    更新日期:2007-04-01 00:00:00

  • Functional analysis of Borrelia burgdorferi uvrA in DNA damage protection.

    abstract::Bacterial pathogens face constant challenges from DNA-damaging agents generated by host phagocytes. Although Borrelia burgdorferi appears to have much fewer DNA repair enzymes than pathogens with larger genomes, it does contain homologues of uvrA and uvrB (subunits A and B of excinuclease ABC). As a first step to expl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sambir M,Ivanova LB,Bryksin AV,Godfrey HP,Cabello FC

    更新日期:2011-04-01 00:00:00

  • Plasmid-encoded gamma-hexachlorocyclohexane degradation genes and insertion sequences in Sphingobium francense (ex-Sphingomonas paucimobilis Sp+).

    abstract::The lin genes encode the gamma-hexachlorocyclohexane (gamma-HCH or lindane) catabolic pathway in lindane-degrading strains. The location and stability of these genes have been explored in the lindane-degrading Sphingobium francense strain Sp+, and in two non-lindane-degrading mutants (Sp1- and Sp2-). The lin genes, li...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cérémonie H,Boubakri H,Mavingui P,Simonet P,Vogel TM

    更新日期:2006-04-01 00:00:00

  • Analysis of low-molecular mass products from biosolubilized coal.

    abstract::A relatively simple, rapid sample preparation method has been developed for analysis of low-molecular mass compounds present in soluble coal products generated by microbial coal solubilizing agents. Acidification of the sample followed by direct extraction into hexanes is coupled with gas chromatography/mass spectrome...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Toth-Allen J,Torzilli AP,Isbister JD

    更新日期:1994-03-01 00:00:00

  • Genotypic variation in 'Campylobacter upsaliensis' from blood and faeces of patients in different countries.

    abstract::Chromosomal DNA from 62 strains of 'Campylobacter upsaliensis' from blood and faecal specimens of patients in Australia, Belgium, South Africa and England, and seven dog isolates from England and Sweden were examined. DNA base compositions of 21 strains were 35 +/- 1 mol% G + C. Electrophoresis of HaeIII DNA digests g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Owen RJ,Hernandez J

    更新日期:1990-10-01 00:00:00

  • Functional and physiological evidence for a rhesus-type ammonia transporter in Nitrosomonas europaea.

    abstract::Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiologica...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Weidinger K,Neuhäuser B,Gilch S,Ludewig U,Meyer O,Schmidt I

    更新日期:2007-08-01 00:00:00

  • Experimental evidence of an acetate transporter protein and characterization of acetate activation in aceticlastic methanogenesis of Methanosarcina mazei.

    abstract::Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigated ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Welte C,Kröninger L,Deppenmeier U

    更新日期:2014-10-01 00:00:00

  • Influence of lipopolysaccharide chemotype on the interaction between Klebsiella pneumoniae and human polymorphonuclear leucocytes.

    abstract::A series of isogenic mutants lacking either the O1 (O-:K66) or K66 (O1:K-) antigens or both (O-:K-), some of which had additional defects in their LPS core polysaccharide was used to examine the interaction between polymorphonuclear leucocytes (PMNLs) and K. pneumoniae serotype O1:K66. In the absence of serum compleme...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Williams P,Ciurana B,Camprubi S,Tomas JM

    更新日期:1990-06-01 00:00:00

  • Prevalence of a Vibrio cholerae 18-kDa antigen in vibrios.

    abstract::An 18-kDa protein that occurs in Vibrio cholerae has been described as an in vivo and low-iron regulated outer membrane antigen. Monoclonal antibodies which recognized this antigen were protective as passive vaccines in the infant rabbit model of cholera disease. In this study, those monoclonal antibodies were used in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sciortino CV Jr

    更新日期:1993-07-15 00:00:00

  • Enzymatic cocktails produced by Fusarium graminearum under submerged fermentation using different lignocellulosic biomasses.

    abstract::Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Debeire P,Delalande F,Habrylo O,Jeltsch JM,Van Dorsselaer A,Phalip V

    更新日期:2014-06-01 00:00:00

  • Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus.

    abstract::Expression of AT-rich genes from microorganisms such as archaea is often inefficient in Escherichia coli. The trpA and trpB genes encoding the tryptophan synthase subunits were cloned from the hyperthermophilic archaeon Pyrococcus furiosus. No apparent difference in codon bias was found between the genes. However, usi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ishida M,Oshima T,Yutani K

    更新日期:2002-11-05 00:00:00

  • Ability of human sera to neutralise the activity of Vero cytotoxins VT1, VT2 and variant forms of VT2.

    abstract::Sera, from 17 patients with diarrhoea or haemolytic uraemic syndrome, and six healthy adults, were tested for neutralisation of Vero cytotoxins (VT). For all 17 patients there was evidence of infection with Escherichia coli O157. Sera from two controls but from none of the patients neutralised VT1, although two patien...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Scotland SM,Said B,Thomas A,Rowe B

    更新日期:1994-01-15 00:00:00

  • Intracellular viability of toxigenic Corynebacterium diphtheriae strains in HEp-2 cells.

    abstract::Corynebacterium diphtheriae, generally considered an extracellular coloniser, was evaluated for its ability to enter and survive within HEp-2 monolayers by gentamicin protection assay. Intracellular viability of HC01 strain, isolated from endocarditis, was more expressive (2.59%) than observed in 241 (0.21%) and CDC-E...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hirata R,Napoleão F,Monteiro-Leal LH,Andrade AF,Nagao PE,Formiga LC,Fonseca LS,Mattos-Guaraldi AL

    更新日期:2002-09-24 00:00:00

  • Draft genome sequences of three Holospora species (Holospora obtusa, Holospora undulata, and Holospora elegans), endonuclear symbiotic bacteria of the ciliate Paramecium caudatum.

    abstract::We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum; that is, the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata and H. elegans. We investigate functions of orthologous core genes conserved across the three Holospora species, which may be e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dohra H,Tanaka K,Suzuki T,Fujishima M,Suzuki H

    更新日期:2014-10-01 00:00:00

  • Effect of Urtica dioica agglutinin and Arabidopsis thaliana Chia4 chitinase on the protozoan Phytomonas françai.

    abstract::The genus Phytomonas is responsible for many diseases in different crop plant species. The finding that chitin is an exposed cell surface polysaccharide in Phytomonas françai and the observation that chitinases can inhibit fungal growth raises expectations about the potential effect of plant chitinases on the P. franç...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gomes Rocha GC,Nicolich R,Romeiro A,Margis-Pinheiro M,Attias M,Alves-Ferreira M

    更新日期:2003-09-12 00:00:00

  • The Salmonella typhimurium flgM gene, which encodes a negative regulator of flagella synthesis and is involved in virulence, is present and functional in other Salmonella species.

    abstract::FlgM inhibits the flagella-specific sigma factor FliA and is involved in the mouse-virulence of Salmonella typhimurium. In recent experiments, we observed that: (i) a flgM gene that could function to negatively regulate flagella synthesis was present in a variety of salmonellae; and (ii) the flgM gene derived from Sal...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schmitt CK,Darnell SC,O'Brien AD

    更新日期:1996-01-15 00:00:00