Expression and activity of the Calvin-Benson-Bassham cycle transcriptional regulator CbbR from Acidithiobacillus ferrooxidans in Ralstonia eutropha.


:Autotrophic fixation of carbon dioxide into cellular carbon occurs via several pathways but quantitatively, the Calvin-Benson-Bassham cycle is the most important. CbbR regulates the expression of the cbb genes involved in CO2 fixation via the Calvin-Benson-Bassham cycle in a number of autotrophic bacteria. A gene potentially encoding CbbR (cbbR(AF)) has been predicted in the genome of the chemolithoautotrophic, extreme acidophile Acidithiobacillus ferrooxidans. However, this microorganism is recalcitrant to genetic manipulation impeding the experimental validation of bioinformatic predictions. Two novel functional assays were devised to advance our understanding of cbbR(AF) function using the mutated facultative autotroph Ralstonia eutropha H14 ΔcbbR as a surrogate host to test gene function: (i) cbbR(AF) was expressed in R. eutropha and was able to complement ΔcbbR; and (ii) CbbR(AF) was able to regulate the in vivo activity of four A. ferrooxidans cbb operon promoters in R. eutropha. These results open up the use of R. eutropha as a surrogate host to explore cbbR(AF) activity.


FEMS Microbiol Lett


Esparza M,Jedlicki E,Dopson M,Holmes DS




Has Abstract


2015-08-01 00:00:00














  • Diversity of endophytic fungi of Paeonia lactiflora Pallas and screening for fungal paeoniflorin producers.

    abstract::Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on morphological traits a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cheng X,Wei Z,Pu S,Xiang M,Yan A,Zhang Y,Wang X

    更新日期:2018-12-01 00:00:00

  • Lactobacillus fermentum 3872 genome sequencing reveals plasmid and chromosomal genes potentially involved in a probiotic activity.

    abstract::In this report we describe a Lactobacillus fermentum 3872 plasmid (pLF3872) not previously found in any other strain of this species. The analysis of the complete sequence of this plasmid revealed the presence of a gene encoding a large collagen-binding protein (CBP), as well as the genes responsible for plasmid maint...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lehri B,Seddon AM,Karlyshev AV

    更新日期:2015-06-01 00:00:00

  • The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov.

    abstract::The phylogenetic interrelationships of the genera Pediococcus and Aerococcus were investigated using reverse transcriptase sequencing of 16S rRNA. The genus Pediococcus was found to be phylogenetically heterogeneous. The four species P. acidilactici, P. damnosus, P. parvulus and P. pentosaceus formed a phylogeneticall...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Collins MD,Williams AM,Wallbanks S

    更新日期:1990-08-01 00:00:00

  • Genetic engineering of Streptococcus gordonii for the simultaneous display of two heterologous proteins at the bacterial surface.

    abstract::The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

    更新日期:2002-04-23 00:00:00

  • Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan.

    abstract::A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17(T)) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17(T) was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g(-1) dry c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Asker D,Beppu T,Ueda K

    更新日期:2007-08-01 00:00:00

  • Presence of non-suppressive, M2-related dsRNAs molecules in Saccharomyces cerevisiae strains isolated from spontaneous fermentations.

    abstract::Total dsRNA extractions in five killer K2 strains of Saccharomyces cerevisiae isolated from spontaneous fermentations revealed the presence of a novel dsRNA fragment (which we named NS dsRNA) of approximately 1.30 kb, together with L and M2 dsRNAs. NS dsRNA appeared to be encapsidated in the same kind of viral particl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cansado J,Barros Velázquez J,Sieiro C,Gacto M,Villa TG

    更新日期:1999-12-15 00:00:00

  • Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66.

    abstract::The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shastry RP,Dolan SK,Abdelhamid Y,Vittal RR,Welch M

    更新日期:2018-05-01 00:00:00

  • The activity and stability of cell-associated activity of bovicin HC5, a bacteriocin from Streptococcus bovis HC5.

    abstract::Streptococcus bovis HC5 cultures released a broad spectrum lantibiotic, bovicin HC5, into the cell-fee culture supernatant after they reached stationary phase, but most of the antibacterial activity remained cell-associated. Cell-associated bovicin HC5 was more resistant to degradation by Pronase E than the cell-free ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Xavier BM,Houlihan AJ,Russell JB

    更新日期:2008-06-01 00:00:00

  • Construction of improved plasmid vectors for promoter characterization in Pseudomonas aeruginosa and other gram-negative bacteria.

    abstract::We report the construction of two broad host range promoter-probe plasmid vectors for rapid analysis of promoters in Gram-negative bacteria. The new vectors, pME4507 and pME4510, carry carbenicillin and gentamycin resistance genes, respectively, and are small sized (4 kb) with a flexible multiple cloning site to facil...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rist M,Kertesz MA

    更新日期:1998-12-01 00:00:00

  • The heme-binding lipoprotein (HbpA) of Haemophilus influenzae: role in heme utilization.

    abstract::Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone to bind heme. Howeve...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Morton DJ,Madore LL,Smith A,Vanwagoner TM,Seale TW,Whitby PW,Stull TL

    更新日期:2005-12-15 00:00:00

  • SopB effector protein of Salmonella Typhimurium is translocated in mesenteric lymph nodes during murine salmonellosis.

    abstract::Salmonella Typhimurium harbors two Salmonella pathogenicity islands (SPIs), each encoding a type three secretion system for virulence proteins. Although there is increasing evidence of postinvasion roles for SPI-1, it has been generally accepted that SPI-1 genes are downregulated following the invasion process. Here, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Giacomodonato MN,Sarnacki SH,Llana MN,Cerquetti MC

    更新日期:2011-04-01 00:00:00

  • Diatom DNA barcodes for forensic discrimination of drowning incidents.

    abstract::The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.I...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Liu M,Zhao Y,Sun Y,Wu P,Zhou S,Ren L

    更新日期:2020-09-01 00:00:00

  • Plasmid transformation and replica filter plating of Acholeplasma laidlawii.

    abstract::The restriction deficient mutant 8195 of Acholeplasma laidlawii strain JA1 was transformed by the promiscuous streptococcal plasmid vector pNZ18 at a frequency of 4 x 10(-4)/cfu. The plasmid was maintained without structural rearrangements but was lost in the absence of a selection pressure, i.e. kanamycin or neomycin...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sundström TK,Wieslander A

    更新日期:1990-10-01 00:00:00

  • Ca2+ stabilization of respiratory complex I from Escherichia coli.

    abstract::Stability of the membrane-bound and purified H+-translocating NADH:ubiquinone oxidoreductase, Complex I, was studied. The loss of the enzyme activity is strongly increased by alkaline pH and dilution of the sample. Complex I inactivation is prevented specifically by a low concentration of Ca2+ and/or an intracellular ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Belevich G,Belevich N,Knuuti J,Verkhovskaya M

    更新日期:2018-06-01 00:00:00

  • Electropulsation as an alternative method for protein extraction from yeast.

    abstract::The application of series of high intensity electric pulses to a yeast suspension provoked a considerable release of some cytoplasmic proteins, glutathione reductase, 3-phosphoglycerate kinase and alcohol dehydrogenase. A maximal yield was achieved 3-8 h after pulsation. The electro-induced protein efflux was accelera...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ganeva V,Galutzov B

    更新日期:1999-05-15 00:00:00

  • Intercellular communication by related bacterial protein toxins: colicins, contact-dependent inhibitors, and proteins exported by the type VI secretion system.

    abstract::Bacteria are in constant conflict with competing bacterial and eukaryotic cells. To cope with the various challenges, bacteria developed distinct strategies, such as toxins that inhibit the growth or kill rivals of the same ecological niche. In recent years, two toxin systems have been discovered - the type VI secreti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Braun V,Patzer SI

    更新日期:2013-08-01 00:00:00

  • Production of volatile organic compounds by mycobacteria.

    abstract::The need for improved rapid diagnostic tests for tuberculosis disease has prompted interest in the volatile organic compounds (VOCs) emitted by Mycobacterium tuberculosis complex bacteria. We have investigated VOCs emitted by Mycobacterium bovis BCG grown on Lowenstein-Jensen media using selected ion flow tube mass sp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: McNerney R,Mallard K,Okolo PI,Turner C

    更新日期:2012-03-01 00:00:00

  • Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge.

    abstract::The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a ti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zhang D,Moreira GS,Shoemaker C,Newton JC,Xu DH

    更新日期:2016-05-01 00:00:00

  • Characterization of pFR18, a small cryptic plasmid from Leuconostoc mesenteroides ssp. mesenteroides FR52, and its use as a food grade vector.

    abstract::A 1.8-kb cryptic plasmid pFR18 was isolated from Leuconostoc mesenteroides ssp. mesenteroides FR52 and characterized. The identification of single-stranded DNA intermediate (ssDNA) in Leuconostoc demonstrated that the replication of pFR18 is directed by a rolling-circle mechanism (RCR). Sequence analysis revealed a si...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Biet F,Cenatiempo Y,Fremaux C

    更新日期:1999-10-15 00:00:00

  • Diversity and distribution of hemerythrin-like proteins in prokaryotes.

    abstract::Hemerythrins are oxygen-binding proteins found in the body fluids and tissues of certain invertebrates. Oxygen is bound at a nonheme iron centre consisting of two oxo-bridged iron atoms bound to a characteristic set of conserved histidine: aspartate and glutamate residues with the motifs H-HxxxE-HxxxH-HxxxxD. It has r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: French CE,Bell JM,Ward FB

    更新日期:2008-02-01 00:00:00

  • Minocycline resistance in an oral Streptococcus infantis isolate is encoded by tet(S) on a novel small, low copy number plasmid.

    abstract::We have determined the genetic basis of minocycline resistance in a strain of Streptococcus infantis isolated from a healthy human oral cavity. We demonstrate that tet(S), identical to tet(S) found on the enterococcal conjugative transposon Tn6000, is responsible for the observed resistance. The gene is located on a s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ciric L,Brouwer MS,Mullany P,Roberts AP

    更新日期:2014-04-01 00:00:00

  • A robust screen for novel antibiotics: specific knockout of the initiator of bacterial DNA replication.

    abstract::We have developed a novel type of a positive screen for the discovery of antibacterial compounds that target the Escherichia coli replication initiator protein DnaA. DnaA is an essential replication protein, conserved in (almost) all bacteria--including all human pathogens--and no existing antibiotics target the main ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fossum S,De Pascale G,Weigel C,Messer W,Donadio S,Skarstad K

    更新日期:2008-04-01 00:00:00

  • Outer membrane proteins of gentamicin induced small colony variants of Pseudomonas aeruginosa.

    abstract::Small colony variants (SCVs) of Pseudomonas aeruginosa NCTC 6750 (WT) were repeatedly isolated in an in vitro kinetic model after exposure to gentamicin (GM). There were minor differences biochemically and in phage and serotyping between the wild type (WT) strain and SCVs. Changes in outer membrane protein profiles we...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Langford PR,Anwar H,Gonda I,Brown MR

    更新日期:1989-10-01 00:00:00

  • Construction of a recombinant wine yeast strain expressing a fungal pectate lyase gene.

    abstract::A gene fusion between the Saccharomyces cerevisiae actin gene promoter and the cDNA of the Fusarium solani f. sp. pisi pelA gene has been constructed. This expression cassette has been introduced into the industrial wine yeast strain T73. The resulting recombinant strain is able to secrete active PELA enzyme into the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: González-Candelas L,Cortell A,Ramon D

    更新日期:1995-03-01 00:00:00

  • Isolation of genes encoding beta-D-xylanase, beta-D-xylosidase and alpha-L-arabinofuranosidase activities from the rumen bacterium Prevotella ruminicola B1(4).

    abstract::Prevotella ruminicola B1(4) is a strictly anaerobic, Gram-negative, polysaccharide-degrading rumen bacterium. Xylanase activity in this strain was found to be inducible, the specific activity of cells grown on xylan being increased at least 20-fold by comparison with cells grown on glucose. Ten bacteriophage clones ex...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gasparic A,Marinsek-Logar R,Martin J,Wallace RJ,Nekrep FV,Flint HJ

    更新日期:1995-01-15 00:00:00

  • Delineation of species within the Trichoderma viride/atroviride/koningii complex by UP-PCR cross-blot hybridization.

    abstract::Sequences from the ribosomal DNA ITS regions have shown that there is limited variation among the species in the Trichoderma viride/atroviride/koningii species complex also known as Hypocrea rufa complex, and that infraspecies variation sometimes is larger than interspecies variation. Strains belonging to T. viride, T...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lübeck M,Bulat S,Alekhina I,Lieckfeldt E

    更新日期:2004-08-15 00:00:00

  • The effect of buthionine sulfoximine on the growth of Leishmania donovani in culture.

    abstract::Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

    更新日期:1999-04-01 00:00:00

  • Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum.

    abstract::A Gram-negative, nonmotile and rod-shaped bacterial strain was isolated from the rhizosphere of Platycodon grandiflorum in a study of bacterial diversity, and its taxonomic position was investigated by a genotypic and phenotypic analysis. This isolate, designated as DR-f4, grew at 4-30 degrees C (optimally at 20-25 de...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kim BC,Lee KH,Kim MN,Lee J,Shin KS

    更新日期:2010-08-01 00:00:00

  • The phylogenetic position of Hydrogenobacter acidophilus based on 16S rRNA sequence analysis.

    abstract::Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shima S,Yanagi M,Saiki H

    更新日期:1994-06-01 00:00:00

  • Characterization of two genes (hupD and hupE) required for hydrogenase activity in Azotobacter chroococcum.

    abstract::In Azotobacter chroococcum the hydrogenase structural genes (hupSL) cover about 2.8 kb of a 15-kb region associated with hydrogen-uptake (Hup) activity. Two other genes in this region, hupD and hupE, were located 8.9 kb downstream of hupL and were shown to be essential for hydrogenase activity by insertion mutagenesis...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Du L,Stejskal F,Tibelius KH

    更新日期:1992-09-01 00:00:00