Expression and activity of the Calvin-Benson-Bassham cycle transcriptional regulator CbbR from Acidithiobacillus ferrooxidans in Ralstonia eutropha.

Abstract:

:Autotrophic fixation of carbon dioxide into cellular carbon occurs via several pathways but quantitatively, the Calvin-Benson-Bassham cycle is the most important. CbbR regulates the expression of the cbb genes involved in CO2 fixation via the Calvin-Benson-Bassham cycle in a number of autotrophic bacteria. A gene potentially encoding CbbR (cbbR(AF)) has been predicted in the genome of the chemolithoautotrophic, extreme acidophile Acidithiobacillus ferrooxidans. However, this microorganism is recalcitrant to genetic manipulation impeding the experimental validation of bioinformatic predictions. Two novel functional assays were devised to advance our understanding of cbbR(AF) function using the mutated facultative autotroph Ralstonia eutropha H14 ΔcbbR as a surrogate host to test gene function: (i) cbbR(AF) was expressed in R. eutropha and was able to complement ΔcbbR; and (ii) CbbR(AF) was able to regulate the in vivo activity of four A. ferrooxidans cbb operon promoters in R. eutropha. These results open up the use of R. eutropha as a surrogate host to explore cbbR(AF) activity.

journal_name

FEMS Microbiol Lett

authors

Esparza M,Jedlicki E,Dopson M,Holmes DS

doi

10.1093/femsle/fnv108

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

fnv108

issue

15

eissn

0378-1097

issn

1574-6968

pii

fnv108

journal_volume

362

pub_type

杂志文章
  • Cloning vectors for Streptococcus thermophilus derived from a native plasmid.

    abstract::A 3.5-kb native plasmid (pND103) was identified in Streptococcus thermophilus ST2-1. Preliminary sequence analysis indicated that pND103 belongs to group I S. thermophilus plasmids. A region of approximately 2 kb appears to contain three components: a plus origin of replication (ori) typical of plasmids that replicate...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11412.x

    authors: Su P,Jury K,Allison GE,Wong WY,Kim WS,Liu CQ,Vancov T,Dunn NW

    更新日期:2002-10-29 00:00:00

  • Comparison of the effect of salinity on the D/H ratio of fatty acids of heterotrophic and photoautotrophic microorganisms.

    abstract::The core metabolism of microorganisms has a major influence on the hydrogen isotopic composition of their fatty acids. Heterotrophic microorganisms produce fatty acids with a deuterium to hydrogen (D/H) ratio either slightly depleted or enriched in D compared to the growth water, while photo- and chemoautotrophic micr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv065

    authors: Heinzelmann SM,Chivall D,M'Boule D,Sinke-Schoen D,Villanueva L,Damsté JS,Schouten S,van der Meer MT

    更新日期:2015-05-01 00:00:00

  • Analysis of hybrids of Candida albicans formed by protoplast fusion.

    abstract::A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06617.x

    authors: Law C,Kavanagh K,Whittaker P

    更新日期:1994-01-01 00:00:00

  • Light-activated adenyl cyclase from Trichoderma viride.

    abstract::The effect of light on adenyl cyclase (E.C. 4.6.1.1) and 3':5'-cyclic-AMP-phosphodiesterase (E.C. 3.1.4.17) activity of Trichoderma viride was investigated. Adenyl cyclase proved to be a membrane-associated enzyme, requiring Mn2+ and was activated by light. In contrast, 3':5'-cyclic-AMP-phosphodiesterase showed no lig...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90474-3

    authors: Kolarova N,Haplová J,Gresík M

    更新日期:1992-06-15 00:00:00

  • Cloning and nucleotide sequence of the Pseudomonas aeruginosa nfxB gene, conferring resistance to new quinolones.

    abstract::The gene nfxB is one of the genes which affect the cell membrane permeability of quinolones in Pseudomonas aeruginosa PAO. Both wild-type nfxB and a mutant nfxB (nfx13E) were cloned and the DNA sequences were determined. The wild-type gene was dominant in PAO strains. The nfxB mutation was a point mutation (cytosine--...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90386-3

    authors: Okazaki T,Hirai K

    更新日期:1992-10-01 00:00:00

  • GeneChip expression analysis of the VqsR regulon of Pseudomonas aeruginosa TB.

    abstract::Two interlinked quorum sensing circuits, las and rhl, which control pathogenesis of Pseudomonas aeruginosa are further modulated by numerous regulators, including VqsR (virulence and quorum sensing regulator). High-density oligonucleotide microarrays were used to compare the global expression profile of a wild-type an...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.11.020

    authors: Juhas M,Wiehlmann L,Salunkhe P,Lauber J,Buer J,Tümmler B

    更新日期:2005-01-15 00:00:00

  • Studies of the subtilin leader peptide as a translocation signal in Escherichia coli K12.

    abstract::Subtilin is an antimicrobial peptide of the lantibiotic family that is produced by Gram-positive Bacillus subtilis, and its biosynthesis involves expression of presubtilin which consists of a leader segment and a mature segment. The leader segment is unlike a typical sec-type general secretion signal, and its ability ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13640.x

    authors: Paul LK,Izaguirre G,Hansen JN

    更新日期:1999-07-01 00:00:00

  • Obligate phototrophy in cyanobacteria: more than a lack of sugar transport.

    abstract::DNA hybridization using the Synechocystis PCC6803 glucose transporter gene, glcP, revealed a single homologous region in two facultative photoautotrophic strains out of three tested, and none in three obligate autotrophs. In one of the latter, Synechococcus PCC7942, integration of glcP into the chromosome resulted in ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12959.x

    authors: Zhang CC,Jeanjean R,Joset F

    更新日期:1998-04-15 00:00:00

  • Yeast genes involved in growth inhibition by Pseudomonas syringae pv. syringae syringomycin family lipodepsipeptides.

    abstract::Saccharomyces cerevisiae genes encoding functions necessary for inhibition by the Pseudomonas syringae pv. syringae cyclic lipodepsipeptide, syringomycin-E, were identified by mutant analyses. Syringomycin-E-resistant mutants were isolated, shown to contain single recessive mutations, and divided into eight gene compl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06595.x

    authors: Takemoto JY,Yu Y,Stock SD,Miyakawa T

    更新日期:1993-12-15 00:00:00

  • Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae.

    abstract::Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic too...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.09.021

    authors: Chopra-Dewasthaly R,Marenda M,Rosengarten R,Jechlinger W,Citti C

    更新日期:2005-12-01 00:00:00

  • Bacterial triterpenoids of the hopane series as biomarkers for the chemotaxonomy of Burkholderia, Pseudomonas and Ralstonia spp.

    abstract::Hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus Pseudomonas. Whereas all strains related to Pseudomonas and Ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the Burkholderia species and in related soil isolates, which contained as m...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08974.x

    authors: Cvejic JH,Putra SR,El-Beltagy A,Hattori R,Hattori T,Rohmer M

    更新日期:2000-02-15 00:00:00

  • The gyrB gene is a useful phylogenetic marker for exploring the diversity of Flavobacterium strains isolated from terrestrial and aquatic habitats in Antarctica.

    abstract::Within the phylum Bacteroidetes, the gyrB gene, encoding for the B subunit of the DNA gyrase, has been used as a phylogenetic marker for several genera closely related to Flavobacterium. The phylogenies of the complete 16S rRNA gene and the gyrB gene were compared for 33 Antarctic Flavobacterium isolates and 23 type s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02326.x

    authors: Peeters K,Willems A

    更新日期:2011-08-01 00:00:00

  • The rhamnolipid stress response of Bacillus subtilis.

    abstract::Rhamnolipids are biosurfactants produced by the soil bacterium P seudomonas aeruginosa. In addition to their high industrial potential as surface-active molecules, rhamnolipids also have antimicrobial properties. In densely populated habitats, such as the soil, production of antimicrobial compounds is important to inh...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02367.x

    authors: Wecke T,Bauer T,Harth H,Mäder U,Mascher T

    更新日期:2011-10-01 00:00:00

  • Genome diversity at the serA-linked capsule locus in Escherichia coli.

    abstract::Individual isolates of Escherichia coli synthesize one of more than 70 chemically distinct polysaccharides which form the capsule. In this article we review the genetics of capsule production in E. coli and highlight what this is beginning to reveal in terms of the genetic basis of the structural diversity of polysacc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1992.tb14029.x

    authors: Boulnois G,Drake R,Pearce R,Roberts I

    更新日期:1992-12-15 00:00:00

  • Cell wall substrate specificity of six different lysozymes and lysozyme inhibitory activity of bacterial extracts.

    abstract::We have investigated the specificity of six different lysozymes for peptidoglycan substrates obtained by extraction of a number of gram-negative bacteria and Micrococcus lysodeikticus with chloroform/Tris-HCl buffer (chloroform/buffer). The lysozymes included two that are commercially available (hen egg white lysozyme...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00240.x

    authors: Nakimbugwe D,Masschalck B,Deckers D,Callewaert L,Aertsen A,Michiels CW

    更新日期:2006-06-01 00:00:00

  • Construction of a food-grade host/vector system for Lactococcus lactis based on the lactose operon.

    abstract::A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-over homologous recombina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07457.x

    authors: MacCormick CA,Griffin HG,Gasson MJ

    更新日期:1995-03-15 00:00:00

  • Widespread ability of fungi to drive quinone redox cycling for biodegradation.

    abstract::Wood-rotting fungi possess remarkably diverse extracellular oxidation mechanisms, including enzymes, such as laccase and peroxidases, and Fenton chemistry. The ability to biologically drive Fenton chemistry by the redox cycling of quinones has previously been reported to be present in both ecologically diverging main ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw105

    authors: Krueger MC,Bergmann M,Schlosser D

    更新日期:2016-06-01 00:00:00

  • Construction of a fusion protein carrying antigenic determinants of enteric clostridial toxins.

    abstract::Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00560-3

    authors: Belyi IF,Varfolomeeva NA

    更新日期:2003-08-29 00:00:00

  • Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh.

    abstract::An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L(-1) FE within 24 h and the metabolite fenoxaprop a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02376.x

    authors: Hou Y,Tao J,Shen W,Liu J,Li J,Li Y,Cao H,Cui Z

    更新日期:2011-10-01 00:00:00

  • Mobilizable narrow host range plasmids as natural suicide vectors enabling horizontal gene transfer among distantly related bacterial species.

    abstract::Klebsiella pneumoniae 287-w carries three small narrow host range (NHR) plasmids (pIGMS31, pIGMS32, and pIGRK), which could be maintained in several closely related species of Gammaproteobacteria, but not in Alphaproteobacteria. The plasmids contain different mobilization systems (MOB), whose activity in Escherichia c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02432.x

    authors: Smorawinska M,Szuplewska M,Zaleski P,Wawrzyniak P,Maj A,Plucienniczak A,Bartosik D

    更新日期:2012-01-01 00:00:00

  • Bacillus cereus and its food poisoning toxins.

    abstract::Bacillus cereus is becoming one of the more important causes of food poisoning in the industrialised world. It produces one emetic toxin and three different enterotoxins. The emetic toxin is a ring-shaped structure of three repeats of four amino and/or oxy acids: [D-O-Leu-D-Ala-L-O-Val-L-Val]3. This ring structure has...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.1997.tb12776.x

    authors: Granum PE,Lund T

    更新日期:1997-12-15 00:00:00

  • Deletion of the C-terminus of polynucleotide phosphorylase increases twitching motility, a virulence characteristic of the anaerobic bacterial pathogen Dichelobacter nodosus.

    abstract::The Gram-negative anaerobe Dichelobacter nodosus is the causative agent of footrot in sheep. Different strains of D. nodosus cause disease of differing severities, ranging from benign to virulent. Virulent strains have greater twitching motility and secrete proteases that are more thermostable than those secreted by b...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01831.x

    authors: Palanisamy SK,Fletcher C,Tanjung L,Katz ME,Cheetham BF

    更新日期:2010-01-01 00:00:00

  • Analysis of the Spirochaeta aurantia flaA gene and transcript.

    abstract::The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a sigma 70-like promoter. The translational start is 31 base pairs after the start of the transcript, the open ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb05971.x

    authors: Parales J Jr,Greenberg EP

    更新日期:1993-02-01 00:00:00

  • Water table related variations in the abundance of intact archaeal membrane lipids in a Swedish peat bog.

    abstract::The presence and distribution of isoprenoid glycerol dialkyl glycerol tetraethers (GDGTs), lipids that constitute the membranes of Archaea, have been investigated in a 50-cm long core from a Swedish peat bog. In the acrotelm, the periodically water saturated and thus oxic upper layer of the peat bog, only minor amount...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.08.012

    authors: Weijers JW,Schouten S,van der Linden M,van Geel B,Damsté JS

    更新日期:2004-10-01 00:00:00

  • Has the prehistoric ice-man contributed to the preservation of living fungal spores?

    abstract::There are arguments against the conclusion drawn by Haselwandter and Ebner that fungal spores have survived for some 5300 years on hay padding in the leather boots of a frozen body discovered in the Austrian Alps. According to cryobiological experience, long-term survival of fungal spores is very unlikely at temperatu...

    journal_title:FEMS microbiology letters

    pub_type: 评论,杂志文章

    doi:10.1111/j.1574-6968.1994.tb06998.x

    authors: Gams W,Stalpers JA

    更新日期:1994-07-01 00:00:00

  • Role of Escherichia coli DnaK and DnaJ chaperones in spontaneous and induced mutagenesis and their effect on UmuC stability.

    abstract::The frequency of spontaneous as well as induced reversions of auxotrophic mutations in Escherichia coli AB1157 and its DeltadnaK and DeltadnaKdnaJ derivatives was estimated. The obtained results demonstrate that both mutants tested are characterized by elevated frequency of spontaneous reversions compared to their AB1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.11.037

    authors: Grudniak AM,Kuć M,Wolska KI

    更新日期:2005-01-15 00:00:00

  • Mutagenic analysis of putative domain II and surface residues in mosquitocidal Bacillus thuringiensis Cry19Aa toxin.

    abstract::The mosquitocidal crystal protein, Cry19Aa, from Bacillus thuringiensis ssp. jegathesan, has high toxicity to Anopheles stephensi and Culex pipiens but is less toxic to Aedes aegypti. To study the functional role of putative domain II and surface residues in mosquito toxicity, 16 alanine substitution mutations were in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01583.x

    authors: Roh JY,Nair MS,Liu XS,Dean DH

    更新日期:2009-06-01 00:00:00

  • The periodontal pathogen Porphyromonas gingivalis harnesses the chemistry of the mu-oxo bishaem of iron protoporphyrin IX to protect against hydrogen peroxide.

    abstract::The major haem component in the black pigment of Porphyromonas gingivalis is the mu-oxo bishaem of iron protoporphyrin IX and formation and cell-surface binding of this haem species is proposed as an extracellular buffer against reactive oxidants [Smalley, J.W. et al. (1998) Biochem. J. 331, 681-685]. P. gingivalis ce...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08951.x

    authors: Smalley JW,Birss AJ,Silver J

    更新日期:2000-02-01 00:00:00

  • Test parameters and cell chain length of Streptococcus thermophilus affect the microbial adhesion to hydrocarbons assay: a methodical approach.

    abstract::The microbial adhesion to hydrocarbons (MATH) test is one of the most common method to determine the hydrophobicity of cell surfaces. Despite its prevalence, no standard test parameters are used in literature, making a direct comparison of data almost impossible. Criticism also focuses on test parameters that may mask...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz150

    authors: Nachtigall C,Weber C,Rothenburger S,Jaros D,Rohm H

    更新日期:2019-06-01 00:00:00

  • Evidence for inter- and intraspecies biofilm formation variability among a small group of coagulase-negative staphylococci.

    abstract::Coagulase-negative staphylococci (CoNS) are common bacterial colonizers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS sp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv175

    authors: Oliveira F,Lima CA,Brás S,França Â,Cerca N

    更新日期:2015-10-01 00:00:00