Molecular mechanism of MexR-mediated regulation of MexAB-OprM efflux pump expression in Pseudomonas aeruginosa.

Abstract:

:To elucidate the molecular mechanism by which the MexR repressor regulates expression of the MexAB-OprM efflux pump, we investigated MexR and the mexR-mexA intergenic DNA (mexOP) interaction, and transcription of the mexA-lacZ reporter gene containing different lengths of mexOP. Homogeneously purified MexR bound specifically to mexOP proximal to mexR. The mexOP-lacZ fusion gene lacking the region immediately proximal to mexR showed minimum enzyme activity, thereby suggesting that a promoter element is located between mexR and the MexR-binding sites. These observations explain the mechanism of self-regulation of mexR expression as well as low and elevated expression of MexAB-OprM in the wild-type strain and nalB-type mutant, respectively, of Pseudomonas aeruginosa.

journal_name

FEMS Microbiol Lett

authors

Saito K,Eda S,Maseda H,Nakae T

doi

10.1111/j.1574-6968.2001.tb10492.x

keywords:

subject

Has Abstract

pub_date

2001-02-05 00:00:00

pages

23-8

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(00)00539-5

journal_volume

195

pub_type

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