Determination of viridans streptococci surface lipoteichoic acid by enzyme linked immune sorbent assay.

abstract：:Amanita exitialis Zhu L. Yang and T.H. Li is a lethal mushroom species recently isolated in Guangdong Province, China. In this report, a pure culture of this species was obtained for the first time. To confirm the identity of the pure culture, the internal transcribed spacer regions of the nuclear ribosomal DNA of the...

journal_title：FEMS microbiology letters

authors： Zhang P,Chen Z,Hu J,Wei B,Zhang Z,Hu W

更新日期：2005-11-15 00:00:00

abstract：:Individual isolates of Escherichia coli synthesize one of more than 70 chemically distinct polysaccharides which form the capsule. In this article we review the genetics of capsule production in E. coli and highlight what this is beginning to reveal in terms of the genetic basis of the structural diversity of polysacc...

journal_title：FEMS microbiology letters

authors： Boulnois G,Drake R,Pearce R,Roberts I

更新日期：1992-12-15 00:00:00

abstract：:Bananas contain large quantities of neurochemicals. Extracts from the peel and pulp of bananas in increasing stages of ripening were prepared and evaluated for their ability to modulate the growth of non-pathogenic and pathogenic bacteria. Extracts from the peel, and to a much lesser degree the pulp, increased the gro...

journal_title：FEMS microbiology letters

authors： Lyte M

更新日期：1997-09-15 00:00:00

abstract：:A molecular cloning strategy has been designed to isolate the gene that encodes the small cytoplasmic RNA (scRNA) component of bacterial signal recognition particles. Using this strategy a putative Listeria monocytogenes scRNA lambda gt11 recombinant clone was isolated. A previously described complementation assay dev...

journal_title：FEMS microbiology letters

authors： Barry T,Kelly M,Glynn B,Peden J

更新日期：1999-04-01 00:00:00

abstract：:We screened for a gene that inhibits streptomycin production in Streptomyces griseus when it is introduced on a high-copy-number plasmid pIJ702, and obtained a plasmid pKM545. The introduction of pKM545 abolished streptomycin production on all media tested including YMP-sugar and Nutrient broth. S1 protection analysis...

journal_title：FEMS microbiology letters

authors： Takano H,Toriumi N,Hirata M,Amano T,Ohya T,Shimada R,Kusada H,Amano S,Matsuda K,Beppu T,Ueda K

更新日期：2016-07-01 00:00:00

abstract：:pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator...

journal_title：FEMS microbiology letters

authors： Yokoyama E,Matsuzaki Y,Doi K,Ogata S

更新日期：1998-12-01 00:00:00

abstract：:In this study we have investigated the ability of nonencapsulated, nontypeable Haemophilus influenzae, NT477 to survive in the J774 mouse macrophage-like cell line. Viable, intracellular nontypeable H. influenzae could still be recovered from macrophages 72 h after phagocytosis. In contrast, H. influenzae strain Rd, a...

journal_title：FEMS microbiology letters

authors： Craig JE,Cliffe A,Garnett K,High NJ

更新日期：2001-09-11 00:00:00

abstract：:Protein interactions among RNA polymerase small subunits from the archaeon Methanococcus jannaschii were investigated using affinity pulldown assays in pairwise and higher-order combinations. In the most extensive study of archaeal RNA polymerase subunit interactions to date, including 37 pairs of proteins, 10 ternary...

journal_title：FEMS microbiology letters

authors： Best AA,Olsen GJ

更新日期：2001-02-05 00:00:00

abstract：:Lineage-specific genes (LSGs) are defined as genes with sequences that are not significantly similar to those in any other lineage. LSGs have been proposed, and sometimes shown, to have significant effects in the evolution of biological function. In this study, two sets of Hanseniaspora spp. LSGs were identified by co...

journal_title：FEMS microbiology letters

authors： Chen K,Tian Z,Chen P,He H,Jiang F,Long CA

更新日期：2020-06-01 00:00:00

abstract：:Phylogenetic relationships between Tuber pseudoexcavatum and other Tuber species were investigated by studying the sequences of four genes: 5.8S-ITS2, beta-tubulin, protein kinase C and elongation factor 1alpha. The four phylogenetic trees allowed to differentiate the black truffle clade, composed of two subclades, on...

journal_title：FEMS microbiology letters

authors： Wang Y,Tan ZM,Zhang DC,Murat C,Jeandroz S,Le Tacon F

更新日期：2006-06-01 00:00:00

abstract：:The diheme cytochromes c of the widespread TsdA family are bifunctional thiosulfate dehydrogenase/tetrathionate reductases. Here, biochemical information was collected about TsdA from the Epsilonproteobacterium Wolinella succinogenes (WsTsdA). The situation in W. succinogenes is unique since TsdA is closely associated...

journal_title：FEMS microbiology letters

authors： Kurth JM,Schuster A,Seel W,Herresthal S,Simon J,Dahl C

更新日期：2017-02-01 00:00:00

abstract：:We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates wa...

journal_title：FEMS microbiology letters

authors： Tola S,Idini G,Manunta D,Casciano I,Rocchigiani AM,Angioi A,Leori G

更新日期：1996-10-01 00:00:00

abstract：:Escherichia coli heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically eng...

journal_title：FEMS microbiology letters

authors： Marcello A,Loregian A,Palù G,Hirst TR

更新日期：1994-03-15 00:00:00

abstract：:Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) protocol to target a specific sequence in the gene coding for the A subunit of Escherichia coli verotoxin (VTe-variant, VTev). This PCR protocol permits the VTe-variant target sequence to be distinguished from closely related sequences in...

journal_title：FEMS microbiology letters

authors： Johnson WM,Tyler SD,Wang G,Lior H

更新日期：1991-11-15 00:00:00

abstract：:Although glycogen and other alpha-1,4-D-glucan storage polysaccharides are present in many bacteria, only few glucan phosphorylases from bacteria have been identified and characterised on the protein or gene level. All bacterial phosphorylases follow the same catalytic mechanisms as their plant and vertebrate counterp...

journal_title：FEMS microbiology letters

authors： Schinzel R,Nidetzky B

更新日期：1999-02-15 00:00:00

abstract：:PM3 is an Aeromonas-specific bacteriophage which was isolated and characterized on A. hydrophila strain TF7. Spontaneous mutants resistant to PM3 were non-motile having lost their characteristic monopolar flagellum. In addition, purified flagella inactivated PM3. PM3 is the first filamentous bacteriophage isolated on ...

journal_title：FEMS microbiology letters

authors： Merino S,Camprubi S,Tomás JM

更新日期：1990-06-01 00:00:00

abstract：:Cytoplasmic inclusions surrounded by a bilayer membrane were seen in thin sections. negatively stained and freeze-fractured preparations of Shewanella putrefaciens. Cells harvested from the late exponential and early stationary phase showed a higher number of these vesicles than bacteria isolated from early exponentia...

journal_title：FEMS microbiology letters

authors： Krause B,Beveridge TJ,Remsen CC,Nealson KH

更新日期：1996-05-15 00:00:00

abstract：:The agp gene encoding the ADP-glucose pyrophosphorylase is involved in cyanobacterial glycogen synthesis and glucosylglycerol formation. By in vitro DNA recombination technology, a mutant with partial deletion of agp gene in the cyanobacterium Synechocystis sp. PCC 6803 was constructed. This mutant could not synthesiz...

journal_title：FEMS microbiology letters

authors： Miao X,Wu Q,Wu G,Zhao N

更新日期：2003-01-21 00:00:00

abstract：:A series of isogenic mutants lacking either the O1 (O-:K66) or K66 (O1:K-) antigens or both (O-:K-), some of which had additional defects in their LPS core polysaccharide was used to examine the interaction between polymorphonuclear leucocytes (PMNLs) and K. pneumoniae serotype O1:K66. In the absence of serum compleme...

journal_title：FEMS microbiology letters

authors： Williams P,Ciurana B,Camprubi S,Tomas JM

更新日期：1990-06-01 00:00:00

abstract：:The mol% G + C of DNA extracted from seven different isolates of Renibacterium salmoninarum was determined. Organisms studied were from selected geographical areas (U.S.A., Canada, England and France) and were isolated from five different species of salmonid fish. The mol% G + C was determined to be 55.5, higher than ...

journal_title：FEMS microbiology letters

authors： Banner CR,Rohovec JS,Fryer JL

更新日期：1991-03-15 00:00:00

abstract：:Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

journal_title：FEMS microbiology letters

authors： Grab DJ,Kennedy R,Philipp MT

更新日期：1996-10-15 00:00:00

abstract：:We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi strain Ty2. The gene showed a high degree of similarity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rfaH mutant was constructed by site-directed mutagenesis. This mutant produced a rough lipopolysa...

journal_title：FEMS microbiology letters

authors： Rojas G,Saldías S,Bittner M,Zaldívar M,Contreras I

更新日期：2001-10-16 00:00:00

abstract：:Crystalline 3-methylaspartase (EC 4.3.1.2) from Escherichia coli strain YG1002 that had been isolated from soil was characterized. The enzyme activity was induced when the organism was grown statically on medium containing (S)-glutamic acid. Its molecular mass is about 84 kDa, and it may be composed of two identical s...

journal_title：FEMS microbiology letters

authors： Asano Y,Kato Y

更新日期：1994-05-15 00:00:00

abstract：:SPC(3) is a multiple antigen peptide derived from the V(3) loop of human immunodeficiency virus (HIV) envelope (Env). It exerts a potent anti-HIV activity whereas it alters neither Env expression nor binding to CD(4). Here, SPC(3) binding characteristics, its subsequent intracellular fate and the fact that it inhibite...

journal_title：FEMS microbiology letters

authors： Barbouche R,Papandréou MJ,Miquelis R,Guieu R,Fenouillet E

更新日期：2000-02-15 00:00:00

abstract：:As shown by both bioassay and high-performance liquid chromatographic (HPLC) analysis, penicillin G production by Aspergillus nidulans is subject to regulation by the pH of the growth medium. Penicillin titres were highest at alkaline pH and in strains carrying mutations in the regulatory gene pacC which mimics the ef...

journal_title：FEMS microbiology letters

authors： Shah AJ,Tilburn J,Adlard MW,Arst HN Jr

更新日期：1991-01-15 00:00:00

abstract：:Deferoxamine (DFO), an FDA-approved iron chelator used for treatment of iron poisoning, affects bacteria as iron availability is intimately connected with growth and several virulence determinants. However, little is known about the effect on oral pathogens. In this study, the effect of DFO on Porphyromonas gingivalis...

journal_title：FEMS microbiology letters

authors： Moon JH,Herr Y,Kim SW,Lee JY

更新日期：2011-10-01 00:00:00

abstract：:A plasmid has been constructed which is a bifunctional vector for Escherichia coli and for streptomycetes, containing the cos site of Streptomyces phage phi C31. It can be efficiently transduced into S. lividans 66 and into several other Streptomyces species. The nucleotide sequence of a 311-bp DNA fragment containing...

journal_title：FEMS microbiology letters

authors： Kobler L,Schwertfirm G,Schmieger H,Bolotin A,Sladkova I

更新日期：1991-03-01 00:00:00

abstract：:Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1...

journal_title：FEMS microbiology letters

authors： Chang B,Yoshida S,Miyamoto H,Ogawa M,Horikawa K,Ogata K,Nishibuchi M,Taniguchi H

更新日期：2000-11-15 00:00:00

abstract：:Rainbow trout gastroenteritis has been related to the accumulation of segmented filamentous bacteria in the digestive tract of fish, which presents lethargy, reduced appetite and accumulation of mucoid faeces. Some authors associate the comparison of illness with the presence of viable filaments, which produce and rel...

journal_title：FEMS microbiology letters

authors： Manzano M,Giusto C,Iacumin L,Patthey C,Cecchini F,Fontanillas R,Comi G

更新日期：2010-07-01 00:00:00

abstract：:The NH(2)-terminal amino acid sequence of L-threo-3-hydroxyaspartate dehydratase from Pseudomonas sp. T62 showed significant similarity to that of the SRY1 gene product of Saccharomyces cerevisiae (serine racemase in yeast). SRY1 was cloned and expressed in Escherichia coli, and the gene product was purified and parti...

journal_title：FEMS microbiology letters

authors： Wada M,Nakamori S,Takagi H

更新日期：2003-08-29 00:00:00