Abstract:
:Crystalline 3-methylaspartase (EC 4.3.1.2) from Escherichia coli strain YG1002 that had been isolated from soil was characterized. The enzyme activity was induced when the organism was grown statically on medium containing (S)-glutamic acid. Its molecular mass is about 84 kDa, and it may be composed of two identical subunits of 42 kDa. The enzyme requires both divalent and monovalent cations such as Mg2+ and K+, respectively. The enzyme catalyzes reversible amination-deamination between mesaconic acid and (2S,3S)-methylaspartic acid, which is the best substrate.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Asano Y,Kato Ydoi
10.1111/j.1574-6968.1994.tb06837.xsubject
Has Abstractpub_date
1994-05-15 00:00:00pages
255-8issue
3eissn
0378-1097issn
1574-6968journal_volume
118pub_type
杂志文章abstract::A lysC-lac'Z fusion plasmid was constructed to study the regulatory region of the lysC gene. Analysis by deletion mutations confirmed the existence of an alternative promoter, P2, located upstream of the previously identified promoter, P1. The transcription start site of promoter P2 was located 85 base pairs upstream ...
journal_title:FEMS microbiology letters
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更新日期:1998-11-01 00:00:00
abstract::A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. Sequence analysis revealed that this cluster contains the genes for the scaffolding protein CipA, the processive endocellulase Cel48A, several endoglucanases of families 5 and 9, the mannanase Man5...
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journal_title:FEMS microbiology letters
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更新日期:1994-10-15 00:00:00
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1993.tb05971.x
更新日期:1993-02-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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pub_type: 杂志文章
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