Abstract:
:Infections caused by Streptococcus suis, a major swine pathogen, include meningitis, arthritis, pneumonia and septicaemia. In this study, we investigated interactions that may occur between human brain microvascular endothelial cells (HBMEC), the main constituent of the blood-brain barrier, and S. suis. We show that S. suis acquires plasmin activity in a time-dependent manner when in contact with cultured HBMEC. Cell-associated plasmin activity reached a plateau following a 48h co-incubation period. Zymography analysis revealed that HBMEC produce urokinase, which is probably involved in activation of plasminogen bound to S. suis. We also show that a S. suis culture supernatant which possesses both phospholipase C and haemolysin (suilysin) activities was able to induce the release of arachidonic acid from the membrane of HBMEC. Evidence suggests that the action of suilysin on HBMEC may be a prerequisite for the action of additional molecules such as phospholipase C. These new biological effects associated with S. suis may play an important role in the migration of S. suis through the blood-brain barrier and in the modulation of local inflammation.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Jobin MC,Fortin J,Willson PJ,Gottschalk M,Grenier Ddoi
10.1016/j.femsle.2005.08.044keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
105-11issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(05)00605-1journal_volume
252pub_type
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