Phospholipid etherlipid and phospholipid fatty acid fingerprints in selected euryarchaeotal monocultures for taxonomic profiling.

abstract：:Periodic evolution of H(2)S during aerobic chemostat culture of Saccharomyces cerevisiae resulted in ultradian metabolic oscillation via periodic inhibition of respiratory activity. To understand the nature of periodic H(2)S evolution, we investigated whether oxidative stress is associated with H(2)S production. The c...

journal_title：FEMS microbiology letters

authors： Kwak WJ,Kwon GS,Jin I,Kuriyama H,Sohn HY

更新日期：2003-02-14 00:00:00

abstract：:A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised against different S. aureus CP5 vaccines in invasion to bovine mammary epithelial cells (MAC-T) and ...

journal_title：FEMS microbiology letters

authors： Renna MS,Pereyra EA,Baravalle C,Camussone CM,Dallard BE,Marcipar IS,Calvinho LF

更新日期：2014-11-01 00:00:00

abstract：:We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to...

journal_title：FEMS microbiology letters

authors： Devillard E,Newbold CJ,Scott KP,Forano E,Wallace RJ,Jouany JP,Flint HJ

更新日期：1999-12-01 00:00:00

abstract：:Endpolygalacturonase I from Stereum purpureum has been identified as a causative substance for the silver-leaf disease in apples. It possesses a unique pro-sequence in the C-terminal region that lacks endpolygalacturonases from any other origin. In this study, we analyzed and compared enzymatic characteristics between...

journal_title：FEMS microbiology letters

authors： Hamada S,Toda K,Ogawa S,Kubota K,Miyairi K

更新日期：2015-09-01 00:00:00

abstract：:A DNA segment located immediately upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7 strain HA1 was cloned and sequenced. This segment contained an open reading frame encoding a predicted protein of 156 amino acids. A database search identified similar open reading frames upstream of the eaeA gene...

journal_title：FEMS microbiology letters

authors： Zhao S,Mitchell SE,Meng J,Doyle MP,Kresovich S

更新日期：1995-11-01 00:00:00

abstract：:The development of natural antibodies to Lactococcus lactis in three inbred strains of mice and the effect of inoculating L. lactis into these mice has been investigated. All animals developed detectable levels of natural (systemic and secretory) anti-lactococcal antibodies. Systemic anti-lactococcal antibodies were p...

journal_title：FEMS microbiology letters

authors： Norton PM,Brown HW,Le Page RW

更新日期：1994-07-15 00:00:00

abstract：:A phage typing system using a group of 11 closely related phage (as judged by Southern analysis and restriction fragment length polymorphism analysis) was able to distinguish at least six phage types in Salmonella heidelberg of human and animal origin. Restriction fragment length polymorphism analysis using cosmid pro...

journal_title：FEMS microbiology letters

authors： Harvey D,Harrington C,Heuzenroeder MW,Murray C

更新日期：1993-04-15 00:00:00

abstract：:The genome sequence of Roseovarius sp. strain 217 indicated that many pathway enzymes found in other organisms for the degradation of taurine are represented, but that a novel, apparently energy-dependent pathway is involved in the conversion of acetyl phosphate to acetyl CoA. Thus, an ABC transporter for taurine coul...

journal_title：FEMS microbiology letters

authors： Baldock MI,Denger K,Smits TH,Cook AM

更新日期：2007-06-01 00:00:00

abstract：:We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters a...

journal_title：FEMS microbiology letters

authors： Fiori PL,Rappelli P,Rocchigiani AM,Cappuccinelli P

更新日期：1993-05-01 00:00:00

abstract：:Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obt...

journal_title：FEMS microbiology letters

authors： Potapov S,Belykh O,Krasnopeev A,Gladkikh A,Kabilov M,Tupikin A,Butina T

更新日期：2018-02-01 00:00:00

abstract：:Helicobacter pylori, a type 1 carcinogen, accounts for numerous gastric cancer-related deaths worldwide. Repurposing existing drugs or developing new ones for a combinatorial approach against increasing antimicrobial resistance is the need of the hour. This study highlights the efficacy of acriflavine hydrochloride (A...

journal_title：FEMS microbiology letters

authors： Tehlan A,Karmakar BC,Paul S,Kumar R,Kaur I,Ghosh A,Mukhopadhyay AK,Dhar SK

更新日期：2020-11-23 00:00:00

abstract：:The fatty acid composition during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes (metacyclogenesis) was analysed by gas-liquid chromatography and mass spectrometry. Significant qualitative and quantitative changes in the fatty acid composition occurred during incubation of epimas...

journal_title：FEMS microbiology letters

authors： Esteves MG,Gonzales-Perdomo M,Alviano CS,Angluster J,Goldenberg S

更新日期：1989-05-01 00:00:00

abstract：:Cell-free protein synthesis has emerged in recent years as a powerful tool that can potentially transform the production of recombinant proteins. Cell-free protein synthesis harnesses the synthetic power of living cells while eliminating many of the constraints of traditional cell-based gene expression methods. Due to...

journal_title：FEMS microbiology letters

authors： Lee KH,Kim DM

更新日期：2018-09-01 00:00:00

abstract：:The intracellular parasite Toxoplasma gondii is known to inhibit apoptosis of its host cell. The molecular mechanisms of this interference are, however, not yet completely understood. We show here that viable parasites prominently inhibited the activation of caspase 3/7 induced by cytochrome c, dATP and dithiothreitol...

journal_title：FEMS microbiology letters

authors： Keller P,Schaumburg F,Fischer SF,Häcker G,Gross U,Lüder CG

更新日期：2006-05-01 00:00:00

abstract：:Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degra...

journal_title：FEMS microbiology letters

authors： Debeire P,Delalande F,Habrylo O,Jeltsch JM,Van Dorsselaer A,Phalip V

更新日期：2014-06-01 00:00:00

abstract：:Bordetella pertussis is the causative agent of whooping cough. Traditional vaccines against this disease are inherently reactogenic, thus research is currently focussed on the production of less reactive, acellular vaccines. Expression of candidate antigens for these vaccines in Escherichia coli would be preferable, h...

journal_title：FEMS microbiology letters

authors： Smith AM,Yan H,Groves N,Dalla Pozza T,Walker MJ

更新日期：2000-10-15 00:00:00

abstract：:Rhizobium sp. SIN-1, isolated in India from root nodules on the tropical legume Sesbania aculeata, also induces nitrogen-fixing nodules on roots of S. macrocarpa, S. speciosa, S. procumbens, S. punicea, S. rostrata, and Vigna unguiculata. Unlike Azorhizobium caulinodans, SIN-1 does not induce stem nodules on S. rostra...

journal_title：FEMS microbiology letters

authors： Rana D,Krishnan HB

更新日期：1995-12-01 00:00:00

abstract：:Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

journal_title：FEMS microbiology letters

authors： Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

更新日期：1999-04-01 00:00:00

abstract：:The enteric microogranisms Salmonella, Escherichia coli and Shigella flexneri prefer to grow in neutral pH environments. They nevertheless experience dramatic pH fluctuations in nature and during pathogenesis. In response to environmental encounters with acid, these organisms have evolved complex, inducible acid survi...

journal_title：FEMS microbiology letters

authors： Bearson S,Bearson B,Foster JW

更新日期：1997-02-15 00:00:00

abstract：:The enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. Most strains able to bind amylase exhibited functional enzyme on their ...

journal_title：FEMS microbiology letters

authors： Douglas CW,Heath J,Gwynn JP

更新日期：1992-04-15 00:00:00

abstract：:We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from...

journal_title：FEMS microbiology letters

authors： Nübel U,Schmidt PM,Reiss E,Bier F,Beyer W,Naumann D

更新日期：2004-11-15 00:00:00

abstract：:Molecular information suggests that there is a broad diversity of acetogens in the rumen, distinct from any currently isolated acetogens. We combined molecular analysis with enrichment culture techniques to investigate this diversity further. Methane-inhibited, hydrogenotrophic enrichment cultures produced acetate as ...

journal_title：FEMS microbiology letters

authors： Gagen EJ,Padmanabha J,Denman SE,McSweeney CS

更新日期：2015-07-01 00:00:00

abstract：:A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 x 10(4) cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with...

journal_title：FEMS microbiology letters

authors： You X,Arrowood MJ,Lejkowski M,Xie L,Schinazi RF,Mead JR

更新日期：1996-03-01 00:00:00

abstract：:The acquisition of tolerance to high hydrostatic pressure of 220 MPa (HHP) in response to a 0.4 mM hydrogen peroxide, 6% ethanol and cold-shock (10 degrees C) pretreatment for different lengths of times was studied in the yeast Saccharomyces cerevisiae. The protection conferred by these different treatments was simila...

journal_title：FEMS microbiology letters

authors： Palhano FL,Orlando MT,Fernandes PM

更新日期：2004-04-01 00:00:00

abstract：:A novel bacterial strain, PM2, capable of growing on methanol, was isolated in alkaline conditions from a soil inoculum. This bacterium was characterized at the physiological, biochemical and molecular level. Based on biochemical and molecular data strain PM2 was classified as a novel member of the group of fluorescen...

journal_title：FEMS microbiology letters

authors： Pacheco CC,Passos JF,Moradas-Ferreira P,De Marco P

更新日期：2003-10-24 00:00:00

abstract：:Molecular microbial ecology studies are heavily reliant on 'Universal' 16S rRNA gene primers for elucidating microbial community structure and composition, and yet primer design and optimization is often overlooked. Primers that exhibit minor biases due to primer-template mismatches can substantially alter the pool of...

journal_title：FEMS microbiology letters

authors： Thomas MC,Thomas DK,Selinger LB,Inglis GD

更新日期：2011-07-01 00:00:00

abstract：:The genus Xanthomonas is composed of several species that cause severe crop losses around the world. In Latin America, one of the most relevant species is Xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. This pathogen causes yield losses in several cultures, including ca...

journal_title：FEMS microbiology letters

authors： Andrade AE,Silva LP,Pereira JL,Noronha EF,Reis FB Jr,Bloch C Jr,dos Santos MF,Domont GB,Franco OL,Mehta A

更新日期：2008-04-01 00:00:00

abstract：:This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expre...

journal_title：FEMS microbiology letters

authors： Balhana RJ,Swanston SN,Coade S,Withers M,Sikder MH,Stoker NG,Kendall SL

更新日期：2013-08-01 00:00:00

abstract：:To improve expression efficiency of the leuB gene of an extreme thermophile. Thermus thermophilus, in Escherichia coli, the gene was placed under a potent promoter, tac. However, the expression was hardly improved, despite increased transcription. The expression under tac promoter was significantly improved by introdu...

journal_title：FEMS microbiology letters

authors： Ishida M,Oshima T

更新日期：1996-01-01 00:00:00

abstract：:The effects of overexpression of the apurinic/apyrimidinic DNA endonuclease Nfo on wet and dry heat and UV-C (254 nm) resistance of Bacillus subtilis spores with or without alpha/beta-type small, acid-soluble spore proteins (SASP) were determined. Results revealed that overexpression of Nfo > or =50-fold in spores inc...

journal_title：FEMS microbiology letters

authors： Barraza-Salas M,Ibarra-Rodríguez JR,Mellado SJ,Salas-Pacheco JM,Setlow P,Pedraza-Reyes M

更新日期：2010-01-01 00:00:00