Phospholipid etherlipid and phospholipid fatty acid fingerprints in selected euryarchaeotal monocultures for taxonomic profiling.


:Phospholipid etherlipid (PLEL) derived isoprenoids and phospholipid fatty acids (PLFA) were determined in eight Euryarchaeotal monocultures for taxonomic profiling. For the first time significant amounts of fatty acids in the PLFA of Euryarchaeota were determined. The PLFA proportion varied between 11.3 and 35.5% of the total phospholipid side chains except in Methanothermus fervidus where PLFA accounted for 89.0% of the total phospholipid side chains. Fractionation of fatty acids prior to gas chromatography mass spectrometry analysis revealed that non-ester-linked fatty acids dominated which accounted for 85.5-95.2% of total PLFA in all investigated archaeal strains. PLEL concentration and composition was estimated in accordance with previous studies with two exceptions. In the polar (phospho)lipid fraction of Methanopyrus kandleri side chains possibly derived from hydroxyarchaeol as well as acyclic and cyclic caldarchaeol were identified. In phospholipid extracts of Methanothermus fervidus the 'H-formed' caldarchaeol could not be detected. Overall, PLEL derived isoprenoids as well as PLFA enabled taxonomic differentiation of the selected microorganisms into phylogenetically related groups.


FEMS Microbiol Lett


Gattinger A,Schloter M,Munch JC





Has Abstract


2002-07-16 00:00:00














  • The restriction endonuclease RflFII, isolated from Ruminococcus flavefaciens FD-1, recognizes the sequence 5'-AGTACT-3', and is inhibited by site-specific adenine methylation.

    abstract::Molecular studies of the rumen bacterium Ruminococcus flavefaciens are constrained by the lack of stable gene transfer systems. We report here on the characterization of RflFII, a restriction endonuclease isolated from R. flavefaciens FD-1. The enzyme is an isoschizomer of ScaI, and cleavage of the DNA is blunt-ended,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Morrison M,Mackie RI,White BA

    更新日期:1994-09-15 00:00:00

  • Isolation of a soluble and membrane-associated Fe(III) reductase from the thermophile, Thermus scotoductus (SA-01).

    abstract::The Fe(III) reductase activity was studied in the South African Fe(III)-reducing bacterium, Thermus scotoductus (SA-01). Fractionation studies revealed that the membrane as well as the soluble fraction contained NAD(P)H-dependent Fe(III) reductase activity. The membrane-associated enzyme was solubilized by KCl treatme...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Möller C,van Heerden E

    更新日期:2006-12-01 00:00:00

  • Characterization of lyso-galactolipids, C-2 and C-3 O-acyl trigalactosylglycerol isomers, obtained from the lichenized fungus Dictyonema glabratum.

    abstract::A mixture of two lyso isomers of a galactolipid was obtained from Dictyonema glabratum. Aqueous hydrolysis gave rise to galactose and glycerol in a 3:1 molar ratio. ESI-MS spectroscopy gave, in the positive-ion mode, a pseudomolecular ion at m/z 839 and daughter ions with m/z 677, 600, 515 and 353, suggesting three ga...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sassaki GL,Gorin PA,Iacomini M

    更新日期:2001-01-15 00:00:00

  • Close relationship of RNase P RNA in Gemmata and anammox planctomycete bacteria.

    abstract::The relationship of RNase P RNA from anammox bacteria 'Candidatus Brocadia anammoxidans' and 'Candidatus Kuenenia stuttgartiensis' with that from other Planctomycetes was investigated. Newly identified rnpB gene sequences were aligned against existing planctomycete RNase P RNA sequences and secondary structures deduce...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Butler MK,Op den Camp HJ,Harhangi HR,Lafi FF,Strous M,Fuerst JA

    更新日期:2007-03-01 00:00:00

  • Bacillus polymyxa stimulates increased Rhizobium etli populations and nodulation when co-resident in the rhizosphere of Phaseolus vulgaris.

    abstract::Microbial competition for carbon sources is a primary determinant of rhizosphere ecology. We employed the PCR to examine the population fluctuations of a symbiotic nitrogen-fixing bacterium (Rhizobium etli) during the first 11 days following inoculation of Phaseolus vulgaris seedlings grown in the presence or absence ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Petersen DJ,Srinivasan M,Chanway CP

    更新日期:1996-09-01 00:00:00

  • Thiabendazole resistance and mutations in the beta-tubulin gene of Penicillium expansum strains isolated from apples and pears with blue mold decay.

    abstract::Penicillium expansum is the causal agent of blue mold rot, a postharvest decay of stored fruits. This fungus also produces the mycotoxins patulin and citrinin. Control of P. expansum still relies mainly on the use of fungicides such as thiabendazole. Since its introduction, resistant strains have been reported. The ai...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cabañas R,Castellá G,Abarca ML,Bragulat MR,Cabañes FJ

    更新日期:2009-08-01 00:00:00

  • Genomic 3' terminal sequence comparison of three isolates of rabbit haemorrhagic disease virus.

    abstract::Comparison of sequence data is necessary in older to investigate virus origins, identify features common to virulent strains, and characterize genomic organization within virus families. A virulent caliciviral disease of rabbits recently emerged in China. We have sequenced 1100 bases from the 3' ends of two independen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Milton ID,Vlasak R,Nowotny N,Rodak L,Carter MJ

    更新日期:1992-05-15 00:00:00

  • Very efficient extracellular production of cholera toxin B subunit using Bacillus brevis.

    abstract::We have constructed a very efficient synthesis and secretion system for cholera toxin B subunit (CTB) of Vibrio cholerae 569B using Bacillus-brevis. The constructed expression-secretion vector has the multiple promoters and the signal peptide coding region of the mwp gene, a structural gene for one of the major cell w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ichikawa Y,Yamagata H,Tochikubo K,Udaka S

    更新日期:1993-08-01 00:00:00

  • Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.

    abstract::In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Duez C,Zervosen A,Teller N,Melkonian R,Banzubazé E,Bouillenne F,Luxen A,Frère JM

    更新日期:2009-11-01 00:00:00

  • Genetic variability among archival cultures of Salmonella typhimurium.

    abstract::The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Edwards K,Linetsky I,Hueser C,Eisenstark A

    更新日期:2001-05-30 00:00:00

  • Construction of a food-grade host/vector system for Lactococcus lactis based on the lactose operon.

    abstract::A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-over homologous recombina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: MacCormick CA,Griffin HG,Gasson MJ

    更新日期:1995-03-15 00:00:00

  • The rhamnolipid stress response of Bacillus subtilis.

    abstract::Rhamnolipids are biosurfactants produced by the soil bacterium P seudomonas aeruginosa. In addition to their high industrial potential as surface-active molecules, rhamnolipids also have antimicrobial properties. In densely populated habitats, such as the soil, production of antimicrobial compounds is important to inh...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wecke T,Bauer T,Harth H,Mäder U,Mascher T

    更新日期:2011-10-01 00:00:00

  • Photoinduced bactericidal activity against Pseudomonas aeruginosa by TiO(2) based thin films.

    abstract::The photoinduced bactericidal capacity of TiO(2) based films was evaluated, using as model organism Pseudomonas aeruginosa. Thin films were obtained by spray pyrolysis; they included undoped, Cu doped, and Al doped TiO(2). Scanning electron microscopy was used to observe the final effect of the irradiated films upon t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Amézaga-Madrid P,Nevárez-Moorillón GV,Orrantia-Borunda E,Miki-Yoshida M

    更新日期:2002-06-04 00:00:00

  • Mycoplasma gallisepticum rpoA gene cluster.

    abstract::Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Skamrov A,Feoktistova E,Goldman M,Beabealashvilli R

    更新日期:2002-03-05 00:00:00

  • Chimeric Ply187 endolysin kills Staphylococcus aureus more effectively than the parental enzyme.

    abstract::Peptidoglycan hydrolases are an effective new source of antimicrobials. A chimeric fusion protein of the Ply187 endopeptidase domain and LysK SH3b cell wall-binding domain is a potent agent against Staphylococcus aureus in four functional assays. ...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Mao J,Schmelcher M,Harty WJ,Foster-Frey J,Donovan DM

    更新日期:2013-05-01 00:00:00

  • Extracellular polymeric substances responsible for bacterial adhesion onto solid surface.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tsuneda S,Aikawa H,Hayashi H,Yuasa A,Hirata A

    更新日期:2003-06-27 00:00:00

  • A new value for mol percent guanine + cytosine of DNA for the salmonid fish pathogen Renibacterium salmoninarum.

    abstract::The mol% G + C of DNA extracted from seven different isolates of Renibacterium salmoninarum was determined. Organisms studied were from selected geographical areas (U.S.A., Canada, England and France) and were isolated from five different species of salmonid fish. The mol% G + C was determined to be 55.5, higher than ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Banner CR,Rohovec JS,Fryer JL

    更新日期:1991-03-15 00:00:00

  • Identification by Tn10 transposon mutagenesis of host factors involved in the biosynthesis of K99 fimbriae of Escherichia coli: effect of LPS core mutations.

    abstract::Tn10 transposon mutagenesis of Escherichia coli producing K99 fimbriae was carried out to identify host factors involved in regulation of biosynthesis of fimbriae. Two chromosomal mutants were obtained that showed a strongly reduced cell surface expression of K99 fimbriae upon colony blotting and ELISA. Analysis by in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pilipcinec E,Huisman TT,Willemsen PT,Appelmelk BJ,de Graaf FK,Oudega B

    更新日期:1994-10-15 00:00:00

  • Delineation of molecular species of a family of diacyltrehaloses from Mycobacterium fortuitum by mass spectrometry.

    abstract::A family of 2,3-di-O-acyl trehaloses (DAT), previously identified in Mycobacterium fortuitum, was studied by fast-atom bombardment mass spectrometry to establish the combinations of fatty acyl substituents and, hence, to delineate the molecular species there comprised. The mass spectra indicated the possible existence...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ariza MA,Valero-Guillén PL

    更新日期:1994-06-15 00:00:00

  • A unique and common restriction fragment pattern of the nucleotide sequences homologous to the genome of vf33, a filamentous bacteriophage, in pandemic strains of Vibrio parahaemolyticus O3:K6 O4:K68, and O1:K untypeable.

    abstract::Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chang B,Yoshida S,Miyamoto H,Ogawa M,Horikawa K,Ogata K,Nishibuchi M,Taniguchi H

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  • Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pescaretti Mde L,Morero R,Delgado MA

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  • Pythium glomeratum, a new species isolated from agricultural soil taken in north-eastern France, its ITS region and its comparison with related species.

    abstract::Pythium glomeratum sp. nov. is described here. It was isolated from soil samples taken in the northern France in 1992, was wrongly identified as Pythium heterothallicum and was kept aside. Recently, the ITS region of the rDNA of this oomycete was amplified and sequenced. The differences between the sequence and a more...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Paul B

    更新日期:2003-08-08 00:00:00

  • Accumulation of ppGpp in Streptococcus pyogenes and Streptococcus rattus following amino acid starvation.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Whitehead KE,Webber GM,England RR

    更新日期:1998-02-01 00:00:00

  • Involvement of the mpo operon in resistance to class IIa bacteriocins in Listeria monocytogenes.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Arous S,Dalet K,Héchard Y

    更新日期:2004-09-01 00:00:00

  • A novel circuit overrides Adr1p control during expression of Saccharomyces cerevisiae 2-trans-enoyl-ACP reductase Etr1p of mitochondrial type 2 fatty acid synthase.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gurvitz A

    更新日期:2009-08-01 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cooper RA

    更新日期:1997-01-01 00:00:00

  • Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Althabegoiti MJ,Covelli JM,Pérez-Giménez J,Quelas JI,Mongiardini EJ,López MF,López-García SL,Lodeiro AR

    更新日期:2011-06-01 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Metcalf JS,Beattie KA,Pflugmacher S,Codd GA

    更新日期:2000-08-15 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takashima S,Iikura H,Nakamura A,Masaki H,Uozumi T

    更新日期:1996-12-15 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Santiago-Rodriguez TM,Patrício AR,Rivera JI,Coradin M,Gonzalez A,Tirado G,Cano RJ,Toranzos GA

    更新日期:2014-01-01 00:00:00