Involvement of oxidative stress in the regulation of H(2)S production during ultradian metabolic oscillation of Saccharomyces cerevisiae.

Abstract:

:Periodic evolution of H(2)S during aerobic chemostat culture of Saccharomyces cerevisiae resulted in ultradian metabolic oscillation via periodic inhibition of respiratory activity. To understand the nature of periodic H(2)S evolution, we investigated whether oxidative stress is associated with H(2)S production. The cellular oxidative states represented by intracellular level of lipid peroxides oscillated out of phase with the oscillation of dissolved O(2). Pulse addition of antioxidant, oxidative agent or inhibitor of antioxidation enzymes perturbed metabolic oscillation producing changes in H(2)S evolution. Analysis of H(2)S production profiles during perturbation of oscillation revealed that the amount of H(2)S production is closely linked with cellular oxidative states. Based on these results and our previous reports, we suggest that oxidative stresses result in periodic depletion of glutathione and cysteine, which in turn causes stimulation of the sulfate assimilation pathway and H(2)S production.

journal_name

FEMS Microbiol Lett

authors

Kwak WJ,Kwon GS,Jin I,Kuriyama H,Sohn HY

doi

10.1016/S0378-1097(02)01198-9

keywords:

subject

Has Abstract

pub_date

2003-02-14 00:00:00

pages

99-104

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109702011989

journal_volume

219

pub_type

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