Abstract:
:Periodic evolution of H(2)S during aerobic chemostat culture of Saccharomyces cerevisiae resulted in ultradian metabolic oscillation via periodic inhibition of respiratory activity. To understand the nature of periodic H(2)S evolution, we investigated whether oxidative stress is associated with H(2)S production. The cellular oxidative states represented by intracellular level of lipid peroxides oscillated out of phase with the oscillation of dissolved O(2). Pulse addition of antioxidant, oxidative agent or inhibitor of antioxidation enzymes perturbed metabolic oscillation producing changes in H(2)S evolution. Analysis of H(2)S production profiles during perturbation of oscillation revealed that the amount of H(2)S production is closely linked with cellular oxidative states. Based on these results and our previous reports, we suggest that oxidative stresses result in periodic depletion of glutathione and cysteine, which in turn causes stimulation of the sulfate assimilation pathway and H(2)S production.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Kwak WJ,Kwon GS,Jin I,Kuriyama H,Sohn HYdoi
10.1016/S0378-1097(02)01198-9keywords:
subject
Has Abstractpub_date
2003-02-14 00:00:00pages
99-104issue
1eissn
0378-1097issn
1574-6968pii
S0378109702011989journal_volume
219pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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