A chemiluminescence immunoassay for evaluation of Cryptosporidium parvum growth in vitro.

abstract：:5-Carboxymethyl-2-hydroxymuconic semialdehyde (CHMS) dehydrogenase from Escherichia coli C and Klebsiella pneumoniae M5a1 have been purified and some of their properties studied. The apparent Km values for NAD and CHMS were 11.7 +/- 1.5 microM and 5.2 +/- 1.9 microM, respectively, for the K. pneumoniae enzyme, and 19....

journal_title：FEMS microbiology letters

authors： Fawcett T,Garrido-Pertierra A,Cooper RA

更新日期：1989-02-01 00:00:00

abstract：:Two yeasts from the effluent of a uranium mineral heap were identified as Candida sorbophila and Rhodotorula mucilaginosa. They are well adapted to such an environment as shown by their tolerance to metals and acid pH. However, different mechanisms could be involved in the adaptation: C. sorbophila could be considered...

journal_title：FEMS microbiology letters

authors： de Silóniz MI,Payo EM,Callejo MA,Marquina D,Peinado JM

更新日期：2002-05-07 00:00:00

abstract：:Autotrophic fixation of carbon dioxide into cellular carbon occurs via several pathways but quantitatively, the Calvin-Benson-Bassham cycle is the most important. CbbR regulates the expression of the cbb genes involved in CO2 fixation via the Calvin-Benson-Bassham cycle in a number of autotrophic bacteria. A gene pote...

journal_title：FEMS microbiology letters

authors： Esparza M,Jedlicki E,Dopson M,Holmes DS

更新日期：2015-08-01 00:00:00

abstract：:The filamentous fungus Fusarium lateritium is cyanide tolerant, due partly to the induction of the enzyme cyanide hydratase in the presence of cyanide. This enzyme catalyses the hydration of cyanide to formamide. The expression in Escherichia coli of a cDNA clone encoding cyanide hydratase is described. The cDNA clone...

journal_title：FEMS microbiology letters

authors： Brown DT,Turner PD,O'Reilly C

更新日期：1995-12-15 00:00:00

abstract：:The aecA and aecB loci map at 250 and 290 degrees, respectively, on the Bacillus subtilis chromosomal genetic map. The aecB locus has been proposed as the structural gene for aspartokinase II. From DNA sequence analyses and comparisons to the sequence of the aspartokinase II gene, it can be concluded that the structur...

journal_title：FEMS microbiology letters

authors： Petricek M,Rutberg L,Hederstedt L

更新日期：1989-10-01 00:00:00

abstract：:Ceramide mono (CMH) or dihexoside (CDH) fractions from Trypanosoma cruzi (Dm28c clone) were identified as glucosyl and lactosylceramides containing non-hydroxylated fatty acids. The di-glycosylated form was much more efficiently recognized by sera from T. cruzi-immunized rabbits, indicating that glycosylation influenc...

journal_title：FEMS microbiology letters

authors： Villas-Boas MH,Wait R,Silva RB,Rodrigues ML,Barreto-Bergter E

更新日期：2005-03-01 00:00:00

abstract：:We have re-examined the stringent response of Streptococcus rattus and Streptococcus pyogenes, two organisms that had originally been reported not to accumulate ppGpp following amino acid deprivation. We conclude that ppGpp does accumulate when S. rattus and S. pyogenes are deprived of isoleucine by mupirocin addition...

journal_title：FEMS microbiology letters

authors： Whitehead KE,Webber GM,England RR

更新日期：1998-02-01 00:00:00

abstract：:The amino acid composition and structure of the peptidoglycan from Heliobacillus mobilis was determined by one- and two-dimensional thin-layer chromatography of completely and partially hydrolysed cell wall preparations. The structure was found to be of the A3 gamma type, with L,L-diaminopimelate in position 3, D-alan...

journal_title：FEMS microbiology letters

authors： Pickett MW,Weiss N,Kelly DJ

更新日期：1994-09-15 00:00:00

abstract：:The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidat...

journal_title：FEMS microbiology letters

authors： Koyanagi T,Katayama T,Suzuki H,Kumagai H

更新日期：2004-08-01 00:00:00

abstract：:Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1...

journal_title：FEMS microbiology letters

authors： Chang B,Yoshida S,Miyamoto H,Ogawa M,Horikawa K,Ogata K,Nishibuchi M,Taniguchi H

更新日期：2000-11-15 00:00:00

abstract：:A new rapid and simple method was developed for the detection of Escherichia coli by constructing a recombinant T4 phage carrying the cytochrome c peroxidase gene derived from Saccharomyces cerevisiae (T4ccp) using which, the colorimetric detection of E. coli K12 was examined. The oxidation activity toward the chromog...

journal_title：FEMS microbiology letters

authors： Hoang HA,Abe M,Nakasaki K

更新日期：2014-03-01 00:00:00

abstract：:We constructed mutant genes of Caldococcus noboribetus isocitrate dehydrogenase containing ancestral amino acid residues that were inferred using the maximal likelihood method and a composite phylogenetic tree of isocitrate dehydrogenase and 3-isopropylmalate dehydrogenase. The mutant genes were expressed in Escherich...

journal_title：FEMS microbiology letters

authors： Iwabata H,Watanabe K,Ohkuri T,Yokobori S,Yamagishi A

更新日期：2005-02-15 00:00:00

abstract：:Crystalline 3-methylaspartase (EC 4.3.1.2) from Escherichia coli strain YG1002 that had been isolated from soil was characterized. The enzyme activity was induced when the organism was grown statically on medium containing (S)-glutamic acid. Its molecular mass is about 84 kDa, and it may be composed of two identical s...

journal_title：FEMS microbiology letters

authors： Asano Y,Kato Y

更新日期：1994-05-15 00:00:00

abstract：:The effect of coumarin (1,2 benzopyrone) on glucose utilisation by the anaerobic rumen fungi Neocallimastix frontalis and N. patriciarum has been compared with the effect of p-coumaric acid. Both compounds largely inhibited glucose utilisation by N. patriciarum strain Cx when present in the medium at a concentration o...

journal_title：FEMS microbiology letters

authors： Cansunar E,Richardson AJ,Wallace G,Stewart CS

更新日期：1990-07-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic pathogen that can cause life-threatening infections in patients admitted to intensive care units. Resistance rapidly develops against two drugs of choice: ceftazidime and meropenem. Several therapeutic protocols were compared for reduction in viable cells and limiting develop...

journal_title：FEMS microbiology letters

authors： Feng Y,Bakker RT,van Hest RM,Hodiamont CJ,Brul S,Schultsz C,Ter Kuile BH

更新日期：2017-08-01 00:00:00

abstract：:Type II toxin-antitoxin systems (TAs) are small autoregulated bicistronic operons that encode a toxin protein with the potential to inhibit metabolic processes and an antitoxin protein to neutralize the toxin. Most of the bacterial genomes encode multiple TAs. However, the diversity and accumulation of TAs on bacteria...

journal_title：FEMS microbiology letters

authors： Ramisetty BC,Santhosh RS

更新日期：2016-02-01 00:00:00

abstract：:Growth of a Mycobacterium avium complex serotype 20 rough-colony variant in 1.0% Tween 80 resulted in a smooth-colony morphological conversion that was reversible upon removal of Tween and was not associated with the presence of serotype-specific glycopeptidolipid antigens. Electron microscopic examination suggested t...

journal_title：FEMS microbiology letters

authors： Wright EL,Pourshafie M,Barrow WW

更新日期：1992-11-01 00:00:00

abstract：:The streptokinase gene of Streptococcus equisimilis H46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. The resu...

journal_title：FEMS microbiology letters

authors： Malke H,Mechold U,Gase K,Gerlach D

更新日期：1994-02-01 00:00:00

abstract：:We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters a...

journal_title：FEMS microbiology letters

authors： Fiori PL,Rappelli P,Rocchigiani AM,Cappuccinelli P

更新日期：1993-05-01 00:00:00

abstract：:Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers ...

journal_title：FEMS microbiology letters

authors： Buivydas A,Pasanen T,Senčilo A,Daugelavičius R,Vaara M,Bamford DH

更新日期：2013-06-01 00:00:00

abstract：:Environmental plasmids often expand the metabolic repertoire of bacteria that carry them, but they also interfere with the biochemical and genetic network of the host. The pWW0 plasmid born by Pseudomonas putida mt-2 encodes the TOL pathway for degradation of toluene/m-xylene through production of intermediate compoun...

journal_title：FEMS microbiology letters

authors： Silva-Rocha R,de Lorenzo V

更新日期：2014-07-01 00:00:00

abstract：:Aspergillus parasiticus (255), a non-toxigenic isolate showed the presence of secondary metabolites-aflatoxins (B1, B2, G1, G2) when grown in yeast extract sucrose media but not in basal media, thus demonstrating its toxigenic potential. Native PAGE of the crude protein isolated at different growth periods of A. paras...

journal_title：FEMS microbiology letters

authors： Shashidhar J,Sashidhar RB,Deshpande V

更新日期：2005-10-01 00:00:00

abstract：:Certain Lactobacillus brevis strains are resistant to hop-derived compounds such as isohumulone and are able to grow in beer. In this study, we raised an antiserum against our beer spoilage laboratory strain L. brevis 578 which reacted with 23 of 24 beer spoilers and two of 13 non-spoilers in precipitation reactions u...

journal_title：FEMS microbiology letters

authors： Yasui T,Yoda K

更新日期：1997-06-15 00:00:00

abstract：:Clostridium thermocellum, a Gram-positive, thermophilic anaerobe produces a highly active cellulase system. This system, termed the cellulosome, is a complex composed of at least 14-18 different types of components organized around a large, cellulose-binding protein. Combining recombinant DNA technology and protein bi...

journal_title：FEMS microbiology letters

authors： Béguin P,Millet J,Aubert JP

更新日期：1992-12-15 00:00:00

abstract：:Segmented filamentous bacteria (SFB) are inhabitants of the small intestine of various animals, where they can be detected microscopically due to their specific morphology and intimate association with the intestinal epithelium. SFB colonize the distal part of the small intestine in a host-specific manner and affects ...

journal_title：FEMS microbiology letters

authors： Jonsson H

更新日期：2013-05-01 00:00:00

abstract：:This study was undertaken to investigate the binding activity of Streptococcus suis serotype 2 to albumin. Using flow cytometry we observed a binding activity of S. suis to albumin for virulent as well as for avirulent isolates. Western immunoblots analysis revealed that a 39-kDa S. suis protein was responsible, at le...

journal_title：FEMS microbiology letters

authors： Quessy S,Busque P,Higgins R,Jacques M,Dubreuil JD

更新日期：1997-02-15 00:00:00

abstract：:Phytochromes are red/far-red light photoreceptors found in plants, cyanobacteria and heterotrophic bacteria. Biochemical analyses have established that the genes bphO and bphP (PA4116 and PA4117) of Pseudomonas aeruginosa encode both phytochrome components: BphO, a heme oxygenase that produces the linear tetrapyrrole ...

journal_title：FEMS microbiology letters

authors： Barkovits K,Harms A,Benkartek C,Smart JL,Frankenberg-Dinkel N

更新日期：2008-03-01 00:00:00

abstract：:The hypA-E operon is involved in the maturation of all three NiFe hydrogenases in Escherichia coli. Two hyp promoters have been described; a sigma54-dependent promoter upstream of hypA, and a sigma70-dependent promoter (PhypA) within the hypA coding region. Here it is shown that the oxygen-responsive transcription fac...

journal_title：FEMS microbiology letters

authors： Messenger SL,Green J

更新日期：2003-11-07 00:00:00

abstract：:Stenotrophomonas maltophilia is a multidrug-resistant opportunistic pathogen. S. maltophilia quorum-sensing system is mediated by the diffusible signal factor (DSF), which synthesis depends on rpfF. It has been reported that rpfF disruption in S. maltophilia K279a leads to a loss of DSF synthesis, reduced levels of ex...

journal_title：FEMS microbiology letters

authors： Alcaraz E,García C,Friedman L,de Rossi BP

更新日期：2019-03-01 00:00:00

abstract：:We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum; that is, the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata and H. elegans. We investigate functions of orthologous core genes conserved across the three Holospora species, which may be e...

journal_title：FEMS microbiology letters

authors： Dohra H,Tanaka K,Suzuki T,Fujishima M,Suzuki H

更新日期：2014-10-01 00:00:00