Purification and partial characterization of an antigen specific to Lactobacillus brevis strains with beer spoilage activity.

Abstract:

:Certain Lactobacillus brevis strains are resistant to hop-derived compounds such as isohumulone and are able to grow in beer. In this study, we raised an antiserum against our beer spoilage laboratory strain L. brevis 578 which reacted with 23 of 24 beer spoilers and two of 13 non-spoilers in precipitation reactions using 0.5 M NaOH cell extracts. This specific antigen to the beer spoilage L. brevis strains (SABSL) was demonstrated to be located beneath the S-layer proteins by agglutination reactions using S-layer protein-stripped cells obtained by treatment with 0.1 M NaOH. SABSL was purified using an affinity column coupled with an antibody against SABSL. The purified antigen was hydrolyzed with 2 M HCl and the hydrolyzate was analyzed by thin-layer chromatography and enzymatic analysis. The results showed that SABSL contains glycerol, phosphate, glycerophosphate, D-galactose and D-glucose. D-Galactose and D-glucose accounted for 4.7% and 0.1% of the composition, respectively. Melibiose, but not mannose, inhibited the precipitation reaction. Intense precipitation reactions were obtained with fractions which did not bind to the ConA-column. These results indicate that the immunodominant component of the SABSL is galactose and the SABSL determinant is most probably a galactosylated glycerol teichoic acid. The antiserum raised against the beer spoilage strain L. brevis 578 could distinguish between Pediococcus beer spoilers and non-spoilers in precipitation reactions.

journal_name

FEMS Microbiol Lett

authors

Yasui T,Yoda K

doi

10.1111/j.1574-6968.1997.tb12566.x

subject

Has Abstract

pub_date

1997-06-15 00:00:00

pages

169-76

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(97)00155-9

journal_volume

151

pub_type

杂志文章
  • Mechanisms of action of ostrich beta-defensins against Escherichia coli.

    abstract::To understand their mechanism of antimicrobial activity against Gram-negative bacteria, ostrich beta-defensins, ostricacins-1 and 2 (Osp-1 and Osp-2), were compared with those of sheep myeloid antimicrobial peptide (SMAP)-29 and human neutrophil peptide (HNP)-1, well-characterized sheep alpha-helical and human alpha-d...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00642.x

    authors: Sugiarto H,Yu PL

    更新日期:2007-05-01 00:00:00

  • Relationships between the anti-HIV V(3)-derived peptide SPC(3) and lymphocyte membrane properties involved in virus entry: SPC(3) interferes with CXCR(4).

    abstract::SPC(3) is a multiple antigen peptide derived from the V(3) loop of human immunodeficiency virus (HIV) envelope (Env). It exerts a potent anti-HIV activity whereas it alters neither Env expression nor binding to CD(4). Here, SPC(3) binding characteristics, its subsequent intracellular fate and the fact that it inhibite...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08964.x

    authors: Barbouche R,Papandréou MJ,Miquelis R,Guieu R,Fenouillet E

    更新日期:2000-02-15 00:00:00

  • Development of a species-specific recA-based PCR test for Burkholderia fungorum.

    abstract::The genus Burkholderia comprises over 28 species and species-specific, recA-based polymerase chain reaction (PCR) tests are available for several species, but not for some soil-inhabiting species including B. fungorum. Previous analysis of several novel rhizospheric, environmental isolates belonging to the B. cepacia ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00443-9

    authors: Chan CH,Stead DE,Coutts RH

    更新日期:2003-07-15 00:00:00

  • Serotype F double- and triple-converting phage insertionally inactivate the Staphylococcus aureus beta-toxin determinant by a common molecular mechanism.

    abstract::The precise molecular mechanism of Staphylococcus aureus beta-toxin inactivation by the serotype F triple-converting phage phi 42, phi A1 and phi A3 was investigated. Sequence analysis of the phi 42 (attP) and Staphylococcus aureus (attB) attachment sites and the left (attL) and right (attR) chromosomal/bacteriophage ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb05951.x

    authors: Carroll JD,Cafferkey MT,Coleman DC

    更新日期:1993-01-15 00:00:00

  • Methanogen communities in stools of humans of different age and health status and co-occurrence with bacteria.

    abstract::Hydrogenotrophic methanogens live in a synthrophic relationship with the human gut microbiota as the terminal part of the anaerobic food chain. Methanobrevibacter smithii of the Methanobacteriales is the prevailing archaeal species. Recently, methylotrophic archaea of the novel order Methanomassiliicoccales were isola...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv092

    authors: Vanderhaeghen S,Lacroix C,Schwab C

    更新日期:2015-07-01 00:00:00

  • The emergence of colistin-resistant Escherichia coli in chicken meats in Nepal.

    abstract::The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz237

    authors: Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

    更新日期:2019-10-01 00:00:00

  • Marine phage genomics: the tip of the iceberg.

    abstract::Marine viruses are the most abundant biological entity in the oceans, the majority of which infect bacteria and are known as bacteriophages. Yet, the bulk of bacteriophages form part of the vast uncultured dark matter of the microbial biosphere. In spite of the paucity of cultured marine bacteriophages, it is known th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnw158

    authors: Perez Sepulveda B,Redgwell T,Rihtman B,Pitt F,Scanlan DJ,Millard A

    更新日期:2016-08-01 00:00:00

  • Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7.

    abstract::The molecular mechanisms controlling expression of the long polar fimbriae 2 (Lpf2) of enterohemorrhagic Escherichia coli (EHEC) O157:H7 were evaluated. Primer extension was used to locate the lpfA2 transcriptional start site in EHEC strain EDL933 at 171 bp upstream of the lpfA2 start codon. Semi-quantitative RT-PCR d...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12513

    authors: Arenas-Hernández MM,Rojas-López M,Medrano-López A,Nuñez-Reza KJ,Puente JL,Martínez-Laguna Y,Torres AG

    更新日期:2014-08-01 00:00:00

  • The Bacillus subtilis catabolite control protein CcpA exerts all its regulatory functions by DNA-binding.

    abstract::In Bacillus subtilis, carbon catabolite control is mediated by the regulatory protein CcpA. In addition to loss of catabolite repression, ccpA mutants exhibit a severe growth defect. They are not able to grow with glucose and ammonium as single sources of carbon and nitrogen, respectively. Only ccpA mutant strains car...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10830.x

    authors: Ludwig H,Stülke J

    更新日期:2001-09-11 00:00:00

  • Genotypic variation in 'Campylobacter upsaliensis' from blood and faeces of patients in different countries.

    abstract::Chromosomal DNA from 62 strains of 'Campylobacter upsaliensis' from blood and faecal specimens of patients in Australia, Belgium, South Africa and England, and seven dog isolates from England and Sweden were examined. DNA base compositions of 21 strains were 35 +/- 1 mol% G + C. Electrophoresis of HaeIII DNA digests g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90335-n

    authors: Owen RJ,Hernandez J

    更新日期:1990-10-01 00:00:00

  • Molecular characterisation of rough strains of Vibrio cholerae isolated from diarrhoeal cases in India and their comparison to smooth strains.

    abstract::Sixteen of the 18 Vibrio cholerae rough strains isolated from hospitalised diarrhoea patients were found to contain O1 serotype-specific (wbe) genes and all currently known virulence genes. Expression of the regulatory element ToxR was evident in these strains. Cholera toxin production ability of the rough strains was...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(04)00013-8

    authors: De K,Ramamurthy T,Faruque SM,Yamasaki S,Takeda Y,Nair GB,Nandy RK

    更新日期:2004-03-12 00:00:00

  • Physical and genetic map of Enterococcus faecium ATCC19434 and demonstration of intra- and interspecific genomic diversity in enterococci.

    abstract::A physical map of the Enterococcus faecium ATCC19434 chromosome was constructed by NotI, I-CeuI and Sse8387I. The chromosome was a circular DNA of 2600 kb in size, and contained six rRNA operons (rrn). The locations and orientations of the six rrn operons and 24 different determinants were mapped. Genomes of three add...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11041.x

    authors: Oana K,Okimura Y,Kawakami Y,Hayashida N,Shimosaka M,Okazaki M,Hayashi T,Ohnishi M

    更新日期:2002-02-05 00:00:00

  • Cloning and partial sequence of transferrin-binding protein 2 of Neisseria meningitidis using a novel method: twin N-terminal PCR.

    abstract::The genes encoding transferrin-binding proteins (TBPs) 1 and 2 of Neisseria meningitidis and N. gonorrhoeae were used as model loci in a novel method of cloning (twin N-terminal polymerase chain reaction; TNT-PCR) involving amplification between the 5' ends of two genes. Primers were based on N-terminal amino-acid seq...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(93)90354-5

    authors: Wilton J,Ala'Aldeen D,Palmer HM,Borriello SP

    更新日期:1993-02-15 00:00:00

  • Changes in fatty acid composition associated with differentiation of Trypanosoma cruzi.

    abstract::The fatty acid composition during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes (metacyclogenesis) was analysed by gas-liquid chromatography and mass spectrometry. Significant qualitative and quantitative changes in the fatty acid composition occurred during incubation of epimas...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(89)90453-9

    authors: Esteves MG,Gonzales-Perdomo M,Alviano CS,Angluster J,Goldenberg S

    更新日期:1989-05-01 00:00:00

  • Cross-utilization and expression of outer membrane receptor proteins for siderophore uptake by Diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) gut bacteria.

    abstract::Siderophore production by entomo- and phytopathogens, plus the cross-utilization of these siderophores and expression of outer membrane receptor proteins (OMRPs) by Diamondback moth (DBM) gut bacterial strains, were all examined. All the tested strains grew in the presence of 2, 2'-dipyridyl, and the Brachybacterium s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01350.x

    authors: Indiragandhi P,Anandham R,Madhaiyan M,Kim GH,Sa T

    更新日期:2008-12-01 00:00:00

  • Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus.

    abstract::A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dowex 1 x 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 microM added FeCl3. Hydrolysis of the compou...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90605-n

    authors: Yamamoto S,Fujita Y,Okujo N,Minami C,Matsuura S,Shinoda S

    更新日期:1992-07-01 00:00:00

  • Truncated mutants of the colicin A lysis protein are acylated and processed when overproduced.

    abstract::The lipid modification and processing of truncated mutants of the colicin A lysis protein were observed after overproduction by Escherichia coli bacteria, but at a rate far slower than that of the wild-type. The unmodified precursor form of the mutants was stable over hour(s). The truncated mutants provoked lethality,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06760.x

    authors: Cavard D

    更新日期:1994-04-01 00:00:00

  • Transcription and genetic analyses of a putative N-acetylmuramyl-L-alanine amidase in Borrelia burgdorferi.

    abstract::In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01416.x

    authors: Yang Y,Li C

    更新日期:2009-01-01 00:00:00

  • Efficient extracellular production of hybrid E. coli heat-labile enterotoxin B subunits in a marine Vibrio.

    abstract::Escherichia coli heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically eng...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06741.x

    authors: Marcello A,Loregian A,Palù G,Hirst TR

    更新日期:1994-03-15 00:00:00

  • Expression of micF involved in porin synthesis in Escherichia coli: two distinct cis-acting elements respectively regulate micF expression positively and negatively.

    abstract::micF RNA, whose sequence is highly complementary to a 5'-portion of ompF mRNA, has been implicated in the osmoregulation and thermoregulation of the ompF porin gene in Escherichia coli. To define and characterize cis-acting regulatory regions upstream of the micF promoter, a series of deletions of the micF promoter fu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90440-l

    authors: Takayanagi K,Maeda S,Mizuno T

    更新日期:1991-09-15 00:00:00

  • Structural studies on the Ebola virus matrix protein VP40 indicate that matrix proteins of enveloped RNA viruses are analogues but not homologues.

    abstract::Matrix proteins are the driving force of assembly of enveloped viruses. Their main function is to interact with and polymerize at cellular membranes and link other viral components to the matrix-membrane complex resulting in individual particle shapes and ensuring the integrity of the viral particle. Although matrix p...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.femsle.2004.03.002

    authors: Timmins J,Ruigrok RW,Weissenhorn W

    更新日期:2004-04-15 00:00:00

  • Phylogeny of human intestinal spirochaetes inferred from 16S rDNA sequence comparisons.

    abstract::The sequence of 1383 nucleotides of the DNA encoding 16S rDNA was determined for strains of human intestinal spirochaetes, comprising an unnamed isolate and "Brachyspira aalborgi" NCTC 11492. A phylogenetic tree was inferred from aligned sequence comparisons between the intestinal spirochaetes, representatives of the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06790.x

    authors: Hookey JV,Barrett SP,Reed CS,Barber P

    更新日期:1994-04-15 00:00:00

  • Extreme UV-A sensitivity of the filamentous gliding bacterium Vitreoscilla stercoraria.

    abstract::Strains of the filamentous gliding bacterium Vitreoscilla, LB13 and C1, are shown to be highly sensitive to UV-A (320-400 nm), with an LD50 of less than 20 kJ m(-2). Vitreoscilla LB13 can be protected from UV-A by including superoxide dismutase and catalase, separately or in combination, during the exposure, indicatin...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00420-8

    authors: Biggs LF,Moody AJ

    更新日期:2003-07-15 00:00:00

  • Analysis of low-molecular mass products from biosolubilized coal.

    abstract::A relatively simple, rapid sample preparation method has been developed for analysis of low-molecular mass compounds present in soluble coal products generated by microbial coal solubilizing agents. Acidification of the sample followed by direct extraction into hexanes is coupled with gas chromatography/mass spectrome...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06716.x

    authors: Toth-Allen J,Torzilli AP,Isbister JD

    更新日期:1994-03-01 00:00:00

  • Gram-positive cell wall structure of the A3 gamma type in heliobacteria.

    abstract::The amino acid composition and structure of the peptidoglycan from Heliobacillus mobilis was determined by one- and two-dimensional thin-layer chromatography of completely and partially hydrolysed cell wall preparations. The structure was found to be of the A3 gamma type, with L,L-diaminopimelate in position 3, D-alan...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07135.x

    authors: Pickett MW,Weiss N,Kelly DJ

    更新日期:1994-09-15 00:00:00

  • Molecular analysis of enrichment cultures of marine ammonia oxidisers.

    abstract::Marine ammonia oxidising bacteria were enriched by incubation of sea water, amended with ammonium sulphate, and subsequent subculture in liquid inorganic medium. PCR primers were designed to be specific for rDNA sequences from ammonia oxidisers belonging to the beta-sub-group of the proteobacteria. These primers were ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07059.x

    authors: McCaig AE,Embley TM,Prosser JI

    更新日期:1994-07-15 00:00:00

  • The effect of buthionine sulfoximine on the growth of Leishmania donovani in culture.

    abstract::Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13495.x

    authors: Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

    更新日期:1999-04-01 00:00:00

  • Biological effects of DNA damage in the hyperthermophilic archaeon Sulfolobus acidocaldarius.

    abstract::To investigate the generality of efficient double-strand break repair and damage-induced mutagenesis in hyperthermophilic archaea, we systematically measured the effects of five DNA-damaging agents on Sulfolobus acidocaldarius and compared the results to those obtained for Escherichia coli under corresponding conditio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11056.x

    authors: Reilly MS,Grogan DW

    更新日期:2002-02-19 00:00:00

  • Metagenomics: novel enzymes from non-culturable microbes.

    abstract::In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely unt...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnx211

    authors: Berini F,Casciello C,Marcone GL,Marinelli F

    更新日期:2017-11-15 00:00:00

  • Characterization of a novel vip3-type gene from Bacillus thuringiensis and evidence of its presence on a large plasmid.

    abstract::A novel vip3-type gene named vip3LB has been isolated from Bacillus thuringiensis strain BUPM95. The corresponding secreted vegetative insecticidal protein was active against the lepidopteran insect Ephestia kuehniella. The vip3LB gene was shown, for the first time, to be carried by the large plasmid containing the cr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.02.007

    authors: Mesrati LA,Tounsi S,Jaoua S

    更新日期:2005-03-15 00:00:00