Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus.

Abstract:

:A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dowex 1 x 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 microM added FeCl3. Hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2-ketoglutaric acid. The 1H-NMR spectrum exhibited the presence of a residue from each of these components in the intact molecule. The fast-atom bombardment mass spectrum of the methyl ester derivative indicated a prominent ion at m/z 477, probably corresponding to [M + 1] ion. Other strains of V. parahaemolyticus were also found to produce this compound when grown in an iron-limited medium.

journal_name

FEMS Microbiol Lett

authors

Yamamoto S,Fujita Y,Okujo N,Minami C,Matsuura S,Shinoda S

doi

10.1016/0378-1097(92)90605-n

keywords:

subject

Has Abstract

pub_date

1992-07-01 00:00:00

pages

181-6

issue

1-2

eissn

0378-1097

issn

1574-6968

journal_volume

73

pub_type

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