DNA from Aspergillus flavus contains 5-methylcytosine.

Abstract:

:DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by using a highly sensitive and specific method for detection of modified bases in genomic DNA comprising high-performance liquid chromatography separation of nucleosides, labeling of the nucleoside with deoxynucleoside kinase and two-dimensional thin-layer chromatography. Our results show that 5-methylcytosine is present in DNA from A. flavus. We estimate the relative amounts of 5-methylcytosine to cytosine to be approximately 1/400.

journal_name

FEMS Microbiol Lett

authors

Gowher H,Ehrlich KC,Jeltsch A

doi

10.1111/j.1574-6968.2001.tb10939.x

keywords:

subject

Has Abstract

pub_date

2001-11-27 00:00:00

pages

151-5

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(01)00458-X

journal_volume

205

pub_type

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