Abstract:
:The filamentous fungi Monascus spp., which have been used in traditional fermented food in Asia for centuries, are well-known producers of a group of bioactive metabolites that are widely used as food additives and nutraceutical supplements worldwide. However, its potential to produce the mycotoxin citrinin poses a threat to food safety. Here, a G-protein alpha-subunit-encoding gene, Mga1 (Monascus G-protein alpha-subunit 1), which encodes a protein showing a high degree of identity to Group I alpha-subunits of fungal heterotrimeric G-proteins, was cloned from Monascus ruber M7. An Mga1-disrupted strain was obtained by homologous recombination. The disruptant produced approximately nine times more citrinin and 71% more pigments than the wild-type strain M7, indicating that the G-protein alpha-subunit encoded by Mga1 is involved in a signal transduction pathway regulating citrinin and pigment biosynthesis in M. ruber M7.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Li L,Shao Y,Li Q,Yang S,Chen Fdoi
10.1111/j.1574-6968.2010.01992.xsubject
Has Abstractpub_date
2010-07-01 00:00:00pages
108-14issue
2eissn
0378-1097issn
1574-6968pii
FML1992journal_volume
308pub_type
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