Identification of Mga1, a G-protein alpha-subunit gene involved in regulating citrinin and pigment production in Monascus ruber M7.

Abstract:

:The filamentous fungi Monascus spp., which have been used in traditional fermented food in Asia for centuries, are well-known producers of a group of bioactive metabolites that are widely used as food additives and nutraceutical supplements worldwide. However, its potential to produce the mycotoxin citrinin poses a threat to food safety. Here, a G-protein alpha-subunit-encoding gene, Mga1 (Monascus G-protein alpha-subunit 1), which encodes a protein showing a high degree of identity to Group I alpha-subunits of fungal heterotrimeric G-proteins, was cloned from Monascus ruber M7. An Mga1-disrupted strain was obtained by homologous recombination. The disruptant produced approximately nine times more citrinin and 71% more pigments than the wild-type strain M7, indicating that the G-protein alpha-subunit encoded by Mga1 is involved in a signal transduction pathway regulating citrinin and pigment biosynthesis in M. ruber M7.

journal_name

FEMS Microbiol Lett

authors

Li L,Shao Y,Li Q,Yang S,Chen F

doi

10.1111/j.1574-6968.2010.01992.x

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

108-14

issue

2

eissn

0378-1097

issn

1574-6968

pii

FML1992

journal_volume

308

pub_type

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