Purification and analysis of the antigenicity of a 69,000 Da protein from Bordetella pertussis.

abstract：:A derivative of Staphylococcus simulans biovar staphylolyticus cured of all five plasmids present in the wild-type organism was developed, and the characteristics of extracellular protein production by this plasmidless strain were compared to those of the wild type. Although staphylolytic endopeptidase (lysostaphin) a...

journal_title：FEMS microbiology letters

authors： Nitterauer JD,Heath HE,Heath LS,LeBlanc PA,Sloan GL

更新日期：1991-11-01 00:00:00

abstract：:Enterococcal surface protein (Esp) is a cell wall-associated protein of Enterococcus faecalis that has been identified as a potential virulence factor. We used a mouse model to examine whether Esp facilitates intestinal colonization or translocation of E. faecalis to mesenteric lymph nodes. After clindamycin treatment...

journal_title：FEMS microbiology letters

authors： Pultz NJ,Shankar N,Baghdayan AS,Donskey CJ

更新日期：2005-01-15 00:00:00

abstract：:There are two major pathways for methionine biosynthesis: the enterobacterial type transsulfuration pathway and the sulfhydrylation pathway as previously identified in the spirochete Leptospira meyeri. Sequence analysis of the L. meyeri metYX locus allows the identification of a third gene, called metW, which encodes ...

journal_title：FEMS microbiology letters

authors： Picardeau M,Bauby H,Saint Girons I

更新日期：2003-08-29 00:00:00

abstract：:Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles (Anadara granosa). Thirty-six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these, 21 strains of biot...

journal_title：FEMS microbiology letters

authors： Radu S,Elhadi N,Hassan Z,Rusul G,Lihan S,Fifadara N,Yuherman,Purwati E

更新日期：1998-08-01 00:00:00

abstract：:The main hypothesis of this work was to evaluate the presence of lactic acid bacteria (LAB) intrinsically resistant to plant essential oils in sage (Salvia officinalis L.) and laurel (Laurus nobilis), for future applications in functional cheese production by addition of aromatic herbs. The effect of the drying proces...

journal_title：FEMS microbiology letters

authors： Gaglio R,Catania P,Orlando S,Vallone M,Moschetti G,Settanni L

更新日期：2020-05-01 00:00:00

abstract：:To elucidate the molecular mechanism by which the MexR repressor regulates expression of the MexAB-OprM efflux pump, we investigated MexR and the mexR-mexA intergenic DNA (mexOP) interaction, and transcription of the mexA-lacZ reporter gene containing different lengths of mexOP. Homogeneously purified MexR bound speci...

journal_title：FEMS microbiology letters

authors： Saito K,Eda S,Maseda H,Nakae T

更新日期：2001-02-05 00:00:00

abstract：:Cowpea (Vigna unguiculata) and mung bean (Vigna radiata) are important legume crops yet their rhizobia have not been well characterized. In the present study, 62 rhizobial strains isolated from the root nodules of these plants grown in the subtropical region of China were analyzed via a polyphasic approach. The result...

journal_title：FEMS microbiology letters

authors： Zhang YF,Wang ET,Tian CF,Wang FQ,Han LL,Chen WF,Chen WX

更新日期：2008-08-01 00:00:00

abstract：:To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnos...

journal_title：FEMS microbiology letters

authors： Yokoyama K,Tateishi T,Marumoto T,Saito M

更新日期：2002-07-02 00:00:00

abstract：:The best yield of lipopolysaccharide (LPS) of Pseudomonas pseudomallei GIFU 12046 was obtained by extraction of defatted cells by phenol/chloroform/petroleum ether. The LPS showed a smooth character on SDS-polyacrylamide gel electrophoresis and contained D-glucose, L-glycero-D-manno-heptose, and D-glucosamine as the m...

journal_title：FEMS microbiology letters

authors： Kawahara K,Dejsirilert S,Danbara H,Ezaki T

更新日期：1992-09-15 00:00:00

abstract：:Small plasmids were found in two clinical isolates of Streptococcus pneumoniae from Spain (strains 671 and 678) and in one strain (SpR) isolated in Germany. All three strains contained one plasmid (2.75 to 3.1 kb) which is related to the only previously described pneumococcal plasmid, pDP1. Strains 678 and SpR carried...

journal_title：FEMS microbiology letters

authors： Sibold C,Markiewicz Z,Latorre C,Hakenbeck R

更新日期：1991-01-01 00:00:00

abstract：:The SbmA protein is involved in the transport of MccB17-, MccJ25-, bleomycin- and proline-rich peptides into the Escherichia coli cytoplasm. sbmA gene homologues were found in a variety of bacteria. However, the physiological role of this protein still remains unknown. Previously, we found that a combination of sbmA a...

journal_title：FEMS microbiology letters

authors： Corbalán NS,Adler C,de Cristóbal RE,Pomares MF,Delgado MA,Vincent PA

更新日期：2010-10-01 00:00:00

abstract：:Falcipain-2A, the cysteine protease of Plasmodium falciparum has been proposed as a good drug target. This study evaluated the suitability of Plasmodium berghei as the animal model and reports the first functional expression and characterization of the falcipain-2A orthologue, berghepain-2. Comparative studies reveale...

journal_title：FEMS microbiology letters

authors： Chan C,Goh LL,Sim TS

更新日期：2005-08-15 00:00:00

abstract：:Twelve strains of Candida albicans were grown in defined medium which had been deferrated by ion-exchange chromatography and then supplemented with FeCl3 to give iron concentrations ranging from 0.026 microM (growth-limiting) to 0.8 microM (excess). All of the strains secreted hydroxamate-type siderophores; phenolate ...

journal_title：FEMS microbiology letters

authors： Sweet SP,Douglas LJ

更新日期：1991-05-01 00:00:00

abstract：:Biofilms present complex assemblies of micro-organisms attached to surfaces. they are dynamic structures in which various metabolic activities and interactions between the component cells occur. When phage come in contact with biofilms, further interactions occur dependent on the susceptibility of the biofilm bacteria...

journal_title：FEMS microbiology letters

authors： Sutherland IW,Hughes KA,Skillman LC,Tait K

更新日期：2004-03-12 00:00:00

abstract：:The riboflavin kinase encoding gene ribR is situated within a 12 genes locus ytmI-ytnM of the Bacillus subtilis chromosome. Here we demonstrate that ribR is transcribed as part of a 10 kb ytmI-ytnM operon. The riboflavin overproduction phenotype of B. subtilis ribC mutant strains, which is a result of the strongly red...

journal_title：FEMS microbiology letters

authors： Solovieva IM,Kreneva RA,Errais Lopes L,Perumov DA

更新日期：2005-02-01 00:00:00

abstract：:An obligately anaerobic spirochete designated strain SEBR 4228T (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration) growth range 1.0-10%) at 37 degrees C (growth temperature range 20-40 degrees C...

journal_title：FEMS microbiology letters

authors： Magot M,Fardeau ML,Arnauld O,Lanau C,Ollivier B,Thomas P,Patel BK

更新日期：1997-10-15 00:00:00

abstract：:A gene encoding a phosphomannose isomerase from Streptococcus mutans GS-5 was identified immediately downstream from the fructokinase gene, scrK. Nucleotide sequence analysis of this region revealed an open reading frame (ORF) specifying a putative protein of 316 amino acids. The gene cloned in Escherichia coli expres...

journal_title：FEMS microbiology letters

authors： Sato Y,Yamamoto Y,Kizaki H,Kuramitsu HK

更新日期：1993-11-15 00:00:00

abstract：:The rhl quorum sensing (QS) circuit of Pseudomonas aeruginosa is known to regulate the expression of a number of virulence factors. This study investigates the regulation of rhlI, encoding the auto-inducer synthase RhlI responsible for the synthesis of N-butryl-L-homoserine lactone (BHL). A putative RpoN binding site ...

journal_title：FEMS microbiology letters

authors： Thompson LS,Webb JS,Rice SA,Kjelleberg S

更新日期：2003-03-28 00:00:00

abstract：:We have studied the intraperoxisomal location of catalase in peroxisomes of methanol-grown Hansenula polymorpha by (immuno)cytochemical means. In completely crystalline peroxisomes, in which the crystalline matrix is composed of octameric alcohol oxidase (AO) molecules, most of the catalase protein is located in a nar...

journal_title：FEMS microbiology letters

authors： Keizer I,Roggenkamp R,Harder W,Veenhuis M

更新日期：1992-05-15 00:00:00

abstract：:The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

journal_title：FEMS microbiology letters

authors： Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

更新日期：1995-08-01 00:00:00

abstract：:The Giardia genome project database provides an online resource for Giardia lamblia (WB strain, clone C6) genome sequence information. The database includes edited single-pass reads, the results of BLASTX searches, and details of progress towards sequencing the entire 12 million-bp Giardia genome. Pre-sorted BLASTX re...

journal_title：FEMS microbiology letters

authors： McArthur AG,Morrison HG,Nixon JE,Passamaneck NQ,Kim U,Hinkle G,Crocker MK,Holder ME,Farr R,Reich CI,Olsen GE,Aley SB,Adam RD,Gillin FD,Sogin ML

更新日期：2000-08-15 00:00:00

abstract：:Spores of Bacillus species have novel properties, which allow them to lie dormant for years and then germinate under favourable conditions. In the current work, the role of a key metabolic integrator, coenzyme A (CoA), in redox regulation of growing cells and during spore formation in Bacillus megaterium and Bacillus ...

journal_title：FEMS microbiology letters

authors： Zhyvoloup A,Yu BYK,Baković J,Davis-Lunn M,Tossounian MA,Thomas N,Tsuchiya Y,Peak-Chew SY,Wigneshweraraj S,Filonenko V,Skehel M,Setlow P,Gout I

更新日期：2020-12-22 00:00:00

abstract：:The discovery of nitrite-dependent anaerobic methane oxidation (n-damo) mediated by 'Candidatus Methylomirabilis oxyfera' with nitrite and methane as substrates has connected biogeochemical carbon and nitrogen cycles in a new way. The paddy fields often carry substantial methane and nitrate, thus may be a favorable ha...

journal_title：FEMS microbiology letters

authors： Zhou L,Wang Y,Long XE,Guo J,Zhu G

更新日期：2014-11-01 00:00:00

abstract：:In Bacillus subtilis, carbon catabolite control is mediated by the regulatory protein CcpA. In addition to loss of catabolite repression, ccpA mutants exhibit a severe growth defect. They are not able to grow with glucose and ammonium as single sources of carbon and nitrogen, respectively. Only ccpA mutant strains car...

journal_title：FEMS microbiology letters

authors： Ludwig H,Stülke J

更新日期：2001-09-11 00:00:00

abstract：:Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodula...

journal_title：FEMS microbiology letters

authors： Althabegoiti MJ,Covelli JM,Pérez-Giménez J,Quelas JI,Mongiardini EJ,López MF,López-García SL,Lodeiro AR

更新日期：2011-06-01 00:00:00

abstract：:A DNA microarray was used to analyze the distribution of plasmid and chromosomal genes among strains of enteroaggregative Escherichia coli (EAEC) isolated from a prospective diarrhoea surveillance study in the United Kingdom. Target genes were extracted from existing databases and from the genome sequence of prototype...

journal_title：FEMS microbiology letters

authors： Jenkins C,van Ijperen C,Dudley EG,Chart H,Willshaw GA,Cheasty T,Smith HR,Nataro JP

更新日期：2005-12-01 00:00:00

abstract：:A continuous culture technique utilising a variant of Staphylococcus aureus 8325-4 containing transcriptional gene fusions was used to investigate the relationships between cell density (OD(600)), steady-state specific growth rate (mu) and expression of both agr (accessory gene regulator) and tst (toxic shock syndrome...

journal_title：FEMS microbiology letters

authors： Wright JD,Holland KT

更新日期：2003-01-28 00:00:00

abstract：:Because probiotic effects are strain dependent, genomic explanations of these differences will contribute to understanding their mechanisms of action. The genomic sequence of the Bifidobacterium longum probiotic strain NCC2705 was determined, but little is known about the genetic diversity between strains of this spec...

journal_title：FEMS microbiology letters

authors： Delcenserie V,Lessard MH,LaPointe G,Roy D

更新日期：2008-03-01 00:00:00

abstract：:The genes mphA and mphB encode macrolide 2'-phosphotransferases I and II, respectively, and they confer resistance to macrolide antibiotics in Escherichia coli. To study the expression of these genes in Gram-positive bacteria, we constructed recombinant plasmids that consisted of an mph gene and the pUB110 vector in B...

journal_title：FEMS microbiology letters

authors： Noguchi N,Tamura Y,Katayama J,Narui K

更新日期：1998-02-15 00:00:00

abstract：:Nitrogenase is oxygen-labile. Cyanobacterial heterocysts can fix N(2) in an oxic milieu because their interior is micro-oxic, for which the glycolipid layer of the heterocyst envelope is required. ORF all5341 of the Anabaena sp. genome predicts a glycosyl transferase. An insertional mutant of all5341 synthesized only ...

journal_title：FEMS microbiology letters

authors： Awai K,Wolk CP

更新日期：2007-01-01 00:00:00