The alternative sigma factor RpoN regulates the quorum sensing gene rhlI in Pseudomonas aeruginosa.

abstract：:Acinetobacter baumannii is an important nosocomial pathogen that displays high antibiotic resistance. It causes a variety of infections including pneumonias and sepsis which may result in disseminated intravascular coagulation. In this work, we identify and characterize a novel secreted, zinc-dependent, metallo-endope...

journal_title：FEMS microbiology letters

authors： Tilley D,Law R,Warren S,Samis JA,Kumar A

更新日期：2014-07-01 00:00:00

abstract：:It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, inclu...

journal_title：FEMS microbiology letters

authors： Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

更新日期：2003-04-25 00:00:00

abstract：:An 18-kDa protein that occurs in Vibrio cholerae has been described as an in vivo and low-iron regulated outer membrane antigen. Monoclonal antibodies which recognized this antigen were protective as passive vaccines in the infant rabbit model of cholera disease. In this study, those monoclonal antibodies were used in...

journal_title：FEMS microbiology letters

authors： Sciortino CV Jr

更新日期：1993-07-15 00:00:00

abstract：:Cell differentiation is one of the marks of multicellular organisms. Terminally specialised nitrogen-fixing cells, termed heterocysts, evolved in filamentous cyanobacteria more than 2 Gya. The development of their spacing pattern has been thoroughly investigated in model organisms such as Anabaena sp. PCC 7120. This p...

journal_title：FEMS microbiology letters

authors： Antonaru LA,Nürnberg DJ

更新日期：2017-08-15 00:00:00

abstract：:The mosquitocidal crystal protein, Cry19Aa, from Bacillus thuringiensis ssp. jegathesan, has high toxicity to Anopheles stephensi and Culex pipiens but is less toxic to Aedes aegypti. To study the functional role of putative domain II and surface residues in mosquito toxicity, 16 alanine substitution mutations were in...

journal_title：FEMS microbiology letters

authors： Roh JY,Nair MS,Liu XS,Dean DH

更新日期：2009-06-01 00:00:00

abstract：:The filamentous fungi Monascus spp., which have been used in traditional fermented food in Asia for centuries, are well-known producers of a group of bioactive metabolites that are widely used as food additives and nutraceutical supplements worldwide. However, its potential to produce the mycotoxin citrinin poses a th...

journal_title：FEMS microbiology letters

authors： Li L,Shao Y,Li Q,Yang S,Chen F

更新日期：2010-07-01 00:00:00

abstract：:We have investigated the iron-dependent regulation of the psbA gene, encoding the enzyme L-ornithine N(5)-oxygenase in the rhizobacterium Pseudomonas B10. We have cloned and characterized a Pseudomonas B10 gene, designated psbS, required for psbA expression. PsbS is endowed with structural and functional features of e...

journal_title：FEMS microbiology letters

authors： Leoni L,Ambrosi C,Petrucca A,Visca P

更新日期：2002-03-05 00:00:00

abstract：:Mild alkaline solutions (beta-elimination), after removing the non-covalently bonded wall materials by hot SDS, released 13% and 26% of remaining wall proteins from mycelial and yeast cells of Candida albicans, respectively. When the beta-elimination was carried out after digestion of the walls with chitinase, four-fo...

journal_title：FEMS microbiology letters

authors： Mormeneo S,Marcilla A,Iranzo M,Sentandreu R

更新日期：1994-10-15 00:00:00

abstract：:Efficient type-III secretion depends on cytosolic molecular chaperones, which bind specifically to the translocators and effectors. In the past there has been a tendency to shoe-horn all type-III-secretion chaperones into a single structural and functional class. However, we have shown that the LcrH/SycD-like chaperon...

journal_title：FEMS microbiology letters

authors： Pallen MJ,Francis MS,Fütterer K

更新日期：2003-06-06 00:00:00

abstract：:In this study, we were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis with subpopulations of porcine white blood cells (WBC). The mutants included those with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and a mutant with five major pathogenicity islands remove...

journal_title：FEMS microbiology letters

authors： Stepanova H,Volf J,Malcova M,Matiasovic J,Faldyna M,Rychlik I

更新日期：2011-08-01 00:00:00

abstract：:The major haem component in the black pigment of Porphyromonas gingivalis is the mu-oxo bishaem of iron protoporphyrin IX and formation and cell-surface binding of this haem species is proposed as an extracellular buffer against reactive oxidants [Smalley, J.W. et al. (1998) Biochem. J. 331, 681-685]. P. gingivalis ce...

journal_title：FEMS microbiology letters

authors： Smalley JW,Birss AJ,Silver J

更新日期：2000-02-01 00:00:00

abstract：:To determine whether the luxA-luxB reporter system is suitable as a sensitive reporter for rapid real-time measurements of alpha-toxin gene (cpa) expression in Clostridium perfringens, and to widen the range of alpha-toxin-producing C. perfringens strains examined with respect to cpa expression during growth, the repo...

journal_title：FEMS microbiology letters

authors： Phillips-Jones MK

更新日期：2000-07-01 00:00:00

abstract：:A gene product with an apparent molecular mass of approximately 39,000 Da can be identified in the cytoplasmic membrane of Escherichia coli upon expression of cloned envC. In this communication we report that the product was labelled with [3H]glycerol and [3H]palmitic acid, and a precursor molecule of increased molecu...

journal_title：FEMS microbiology letters

authors： Seiffer D,Klein JR,Plapp R

更新日期：1993-03-01 00:00:00

abstract：:A cryptic plasmid from Arthrobacter rhombi PRH1, designated as pPRH, was sequenced and characterized. It was 5000 bp in length with a G+C content of 66 mol%. The plasmid pPRH was predicted to encode six putative open reading frames (ORFs), in which ORF2 and ORF3 formed the minimal replicon of plasmid pPRH and shared 5...

journal_title：FEMS microbiology letters

authors： Stanislauskiene R,Gasparaviciute R,Vaitekunas J,Meskiene R,Rutkiene R,Casaite V,Meskys R

更新日期：2012-02-01 00:00:00

abstract：:Growth of Prevotella ruminicola strains B(1)4 (subsp. brevis) and D31d (subsp. ruminicola), was inhibited by protamine, a polycationic, low molecular mass protein. Results showed that protamine has a bacteriocidal effect when present in concentrations exceeding 30 micrograms ml-1. Protamine exerted its toxic effects b...

journal_title：FEMS microbiology letters

authors： Madeira HM,Morrison M

更新日期：1997-05-01 00:00:00

abstract：:In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb...

journal_title：FEMS microbiology letters

authors： Yang Y,Li C

更新日期：2009-01-01 00:00:00

abstract：:OMIZ-W68, a chemically defined medium that contains no long-chain fatty acids and yet supports in vitro proliferation of a wide range of fastidious oral anaerobes, is described. The type strains of Treponema denticola, Treponema lecithinolyticum, Treponema maltophilum, Treponema pectinovorum, Treponema socranskii, and...

journal_title：FEMS microbiology letters

authors： Wyss C

更新日期：2007-04-01 00:00:00

abstract：:Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

journal_title：FEMS microbiology letters

authors： Petrovicheva A,Joyner J,Muth TR

更新日期：2017-10-02 00:00:00

abstract：:In order to characterize the metabolites produced in vivo by biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase, the first two enzymes of the (polychloro)biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. LB400, recombinant E. coli strains expressing the respective genes were c...

journal_title：FEMS microbiology letters

authors： Seeger M,Timmis KN,Hofer B

更新日期：1995-11-15 00:00:00

abstract：:Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiologica...

journal_title：FEMS microbiology letters

authors： Weidinger K,Neuhäuser B,Gilch S,Ludewig U,Meyer O,Schmidt I

更新日期：2007-08-01 00:00:00

abstract：:Rhizobium sp. SIN-1, isolated in India from root nodules on the tropical legume Sesbania aculeata, also induces nitrogen-fixing nodules on roots of S. macrocarpa, S. speciosa, S. procumbens, S. punicea, S. rostrata, and Vigna unguiculata. Unlike Azorhizobium caulinodans, SIN-1 does not induce stem nodules on S. rostra...

journal_title：FEMS microbiology letters

authors： Rana D,Krishnan HB

更新日期：1995-12-01 00:00:00

abstract：:The constitutive expression vector pHY300-F1gfp was constructed to test the function of a promoter, F1, cloned from the rice epiphyte Bacillus brevis strain DX01. The DX01 cells harboring the plasmid pHY300-F1gfp were shown to produce bright green fluorescence. The results were confirmed by Western blot analysis and f...

journal_title：FEMS microbiology letters

authors： Chen Y,Yan J,Yang M,Wang J,Shen D

更新日期：2003-12-05 00:00:00

abstract：:Cell walls of Microcystis sp. PCC 7806 were purified from cell homogenates by sucrose density centrifugation and Triton X-100 extraction. The outer membrane contained carotenoids, two major peptidoglycan-associated proteins (Mr 49,000 and 52,000), and lipopolysaccharide (LPS) as indicated by the presence of 3-hydroxy ...

journal_title：FEMS microbiology letters

authors： Jürgens UJ,Martin C,Weckesser J

更新日期：1989-11-01 00:00:00

abstract：:The copZ gene of Bacillus subtilis encodes a copper chaperon CopZ that donates copper to the copper transporter CopA. Both genes copZ and copA are clustered to an operon and its promoter is regulated by Cu ions and CueR, a Mer-like transcriptional activator. Here we show that cadmium ions activate copZA expression as ...

journal_title：FEMS microbiology letters

authors： Solovieva IM,Entian KD

更新日期：2004-07-01 00:00:00

abstract：:One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body. The...

journal_title：FEMS microbiology letters

authors： Magae Y,Hayashi N

更新日期：1999-11-15 00:00:00

abstract：:Saccharomyces cerevisiae genes encoding functions necessary for inhibition by the Pseudomonas syringae pv. syringae cyclic lipodepsipeptide, syringomycin-E, were identified by mutant analyses. Syringomycin-E-resistant mutants were isolated, shown to contain single recessive mutations, and divided into eight gene compl...

journal_title：FEMS microbiology letters

authors： Takemoto JY,Yu Y,Stock SD,Miyakawa T

更新日期：1993-12-15 00:00:00

abstract：:Streptomyces tendae Tü901 produces nikkomycins belonging to the nucleoside peptide antibiotics. Mutants defective in histidine catabolism were isolated and characterized with regard to their histidine ammonium-lyase activity and antibiotic synthesis. In the histidine ammonialyase-negative mutant hut-11 which was unimp...

journal_title：FEMS microbiology letters

authors： Roos U,Mattern S,Schrempf H,Bormann C

更新日期：1992-10-01 00:00:00

abstract：:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

journal_title：FEMS microbiology letters

authors： Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

更新日期：1997-01-15 00:00:00

abstract：:Macrolide is inactivated with ATP plus crude extract of Escherichia coli producing macrolide 2'-phosphotransferase (MPH(2')), but not by living cells. Therefore, a convenient method for detection of MPH(2') using intact cells is needed. In this report, we determine that the modified lysozyme-DNase-RNase (LDR) method (...

journal_title：FEMS microbiology letters

authors： Taniguchi K,Nakamura A,Tsurubuchi K,O'Hara K,Sawai T

更新日期：1998-10-15 00:00:00

abstract：:Plastocyanin, encoded by the petE gene, can transfer electrons to photosystem I (PSI) and cytochrome c oxidase during photosynthetic and respiratory metabolism in cyanobacteria. We constructed a petE mutant of Synechocystis sp. strain PCC 6803 and investigated its phenotypic properties under different light conditions...

journal_title：FEMS microbiology letters

authors： Wang XQ,Jiang HB,Zhang R,Qiu BS

更新日期：2013-04-01 00:00:00