The periodontal pathogen Porphyromonas gingivalis harnesses the chemistry of the mu-oxo bishaem of iron protoporphyrin IX to protect against hydrogen peroxide.

Abstract:

:The major haem component in the black pigment of Porphyromonas gingivalis is the mu-oxo bishaem of iron protoporphyrin IX and formation and cell-surface binding of this haem species is proposed as an extracellular buffer against reactive oxidants [Smalley, J.W. et al. (1998) Biochem. J. 331, 681-685]. P. gingivalis cells grown in the presence of the mu-oxo bishaem were protected against H(2)O(2) compared to control cells grown without it. When added to the growth medium, soluble mu-oxo bishaem inactivated H(2)O(2) and supported cell growth. Cells carrying a surface layer of mu-oxo bishaem were less susceptible to peroxidation by H(2)O(2). Cell-surface haems were slowly destroyed during reaction with H(2)O(2). Binding of mu-oxo bishaem by P. gingivalis may aid survival during neutrophil attack through inactivation of hydrogen peroxide.

journal_name

FEMS Microbiol Lett

authors

Smalley JW,Birss AJ,Silver J

doi

10.1111/j.1574-6968.2000.tb08951.x

keywords:

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

159-64

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(99)00660-6

journal_volume

183

pub_type

杂志文章
  • Induction of L-phenylalanine ammonia-lyase and suppression of veratryl alcohol biosynthesis by exogenously added L-phenylalanine in a white-rot fungus phanerochaete chrysosporium

    abstract::The effects of exogenously added L-phenylalanine (L-Phe) on the activities of L-phenylalanine ammonia-lyase (PAL) and 3, 4-dimethoxybenzyl alcohol (veratryl alcohol, VA) biosynthesis in ligninolytic cultures of Phanerochaete chrysosporium were investigated. Increasing PAL activity was detected in low nitrogen (LN) cul...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb08742.x

    authors: Hattori T,Nishiyama A,Shimada M

    更新日期:1999-10-15 00:00:00

  • Purification and characterization of membrane-bound CO-reactive hemoprotein from Tetrahymena pyriformis mitochondria.

    abstract::A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06423.x

    authors: Inokuchi A,Fukumori Y

    更新日期:1993-08-15 00:00:00

  • Sequence analysis of the 181-kb accessory plasmid pSmeSM11b, isolated from a dominant Sinorhizobium meliloti strain identified during a long-term field release experiment.

    abstract::The 181 251 bp accessory plasmid pSmeSM11b of Sinorhizobium meliloti strain SM11, belonging to a dominant indigenous S. meliloti subpopulation identified during a long-term field release experiment, was sequenced. This plasmid has 166 coding sequences (CDSs), 42% of which encode proteins with homology to proteins of k...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00731.x

    authors: Stiens M,Schneiker S,Pühler A,Schlüter A

    更新日期:2007-06-01 00:00:00

  • Conserved and divergent functions of RcrRPQ in Streptococcus gordonii and S. mutans.

    abstract::In the dental caries pathogen Streptococcus mutans, an MarR-like transcriptional regulator (RcrR), two ABC efflux pumps (RcrPQ) and two effector peptides encoded in the rcrRPQ operon provide molecular connections between stress tolerance, (p)ppGpp metabolism and genetic competence. Here, we examined the role of RcrRPQ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv119

    authors: Shields RC,Burne RA

    更新日期:2015-08-01 00:00:00

  • FNR-mediated regulation of hyp expression in Escherichia coli.

    abstract::The hypA-E operon is involved in the maturation of all three NiFe hydrogenases in Escherichia coli. Two hyp promoters have been described; a sigma54-dependent promoter upstream of hypA, and a sigma70-dependent promoter (PhypA) within the hypA coding region. Here it is shown that the oxygen-responsive transcription fac...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00726-2

    authors: Messenger SL,Green J

    更新日期:2003-11-07 00:00:00

  • Proteomic analysis of the insoluble subproteome of Clostridium difficile strain 630.

    abstract::Clostridium difficile, a Gram-positive spore-forming anaerobe, causes infections in humans ranging from mild diarrhoeal to potentially life-threatening pseudomembranous colitis. The availability of genomic information for a range of C. difficile strains affords researchers the opportunity to better understand not only...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02111.x

    authors: Jain S,Graham RL,McMullan G,Ternan NG

    更新日期:2010-11-01 00:00:00

  • Assembly of the CotSA coat protein into spores requires CotS in Bacillus subtilis.

    abstract::The CotSA protein, encoded by cotSA (ytxN) of Bacillus subtilis, was detected from the cells at 5 h after the onset of sporulation (T5) and in the spore coat of wild-type cells, but not in cotE, cotS, gerE, or cotSA mutant spores. CotSA was also detected in the sporangium at T5 to T7 but not in the sporangium at T18 o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13569.x

    authors: Takamatsu H,Kodama T,Watabe K

    更新日期:1999-05-01 00:00:00

  • The gene encoding the alternative thymidylate synthase ThyX is regulated by sigma factor SigB in Corynebacterium glutamicum ATCC 13032.

    abstract::Both ThyA and ThyX proteins catalyze the transfer of the methyl group from methylenetetrahydrofolate (CH(2) H(4) -folate) to dUMP, forming dTMP. To estimate the relative steady state expression levels of ThyA and ThyX, Western blot analysis was performed using ThyA or ThyX antiserum on total protein from the wild-type...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02494.x

    authors: Cho S,Yang S,Rhie H

    更新日期:2012-03-01 00:00:00

  • Sucrose favors Pseudomonas aeruginosa pellicle production through the extracytoplasmic function sigma factor SigX.

    abstract::Pseudomonas aeruginosa biofilm formation was increased by addition of sucrose to Luria-Bertani medium, whereas addition of NaCl to a final similar osmolarity and use of maltose instead of sucrose, were ineffective. In a previous study, we showed that the extracytoplasmic sigma factor SigX is activated in the presence ...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12482

    authors: Bouffartigues E,Duchesne R,Bazire A,Simon M,Maillot O,Dufour A,Feuilloley M,Orange N,Chevalier S

    更新日期:2014-07-01 00:00:00

  • Tat transport of linker-containing proteins in Escherichia coli.

    abstract::The twin-arginine translocation (Tat) system serves to translocate folded and often cofactor-containing proteins across biological membranes. The mechanistic limits of the Tat system can be explored by addressing the transport of specifically designed Tat substrates. It thus could be recently shown that unstructured p...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01600.x

    authors: Lindenstrauss U,Brüser T

    更新日期:2009-06-01 00:00:00

  • Giardia lamblia -- a model organism for eukaryotic cell differentiation.

    abstract::Giardia lamblia is a binucleated, flagellated protozoan parasite that inhabits the upper small intestine of its vertebrate hosts. The entire life cycle, which can be completed in vitro, is simple with cycling between a vegetative trophozoite and a highly resistant cystic form. The parasite is one of the earliest diver...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2003.tb11490.x

    authors: Svärd SG,Hagblom P,Palm JE

    更新日期:2003-01-21 00:00:00

  • Molecular cloning of a light-inducible gene (lipA) encoding a novel pilin from Arthrobacter photogonimos.

    abstract::Development of pili on cells of Arthrobacter photogonimos is induced by photo-oxidative conditions. The nucleotide sequence was determined of a light-inducible gene (lipA) that encodes the precursor of a light-inducible pilin (designated LIP), a polypeptide of 212 amino acids. The N-terminal leader peptide includes a ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13030.x

    authors: Yang HS,Hoober JK

    更新日期:1998-06-01 00:00:00

  • Use of lectins for a comparative study of cell wall composition of different anaerobic rumen fungal strains.

    abstract::The technique based on fluorescein-linked lectins used to determine the cell wall structure of anaerobic rumen fungi belonging to genera: Neocallimastix, Piromonas and Sphaeromonas, appears to be an interesting tool for distinguishing between strains. Furthermore this technique shows differences of cell wall compositi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1990.tb13853.x

    authors: Guillot J,Breton A,Damez M,Dusser M,Gaillard-Martinie B,Millet L

    更新日期:1990-01-15 00:00:00

  • Mouse pathogenicity studies of Nocardia asteroides complex species and clinical correlation with human isolates.

    abstract::Nocardia asteroides complex organisms derived from human specimens between 1979 and 1992 were identified to the species level. Of 117 N. asteroides complex organisms, 34 (29%) were N. farcinica, 28 (24%) were N. nova, and 55 (47%) were N. asteroides sensu stricto. An analysis of the specimen sites from which the organ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06335.x

    authors: Desmond EP,Flores M

    更新日期:1993-07-01 00:00:00

  • An improved method for detecting cytostatic toxin (emetic toxin) of Bacillus cereus and its application to food samples.

    abstract::We developed an improved HEp-2 cell assay method for the detection of Bacillus cereus toxin, which affects the proliferation of HEp-2 cells. The cytostatic toxin was stable upon exposure to heat, pH 2, pH 11 and trypsin, which suggests it is an emetic. Using the HEp-2 cell assay, we examined the distribution and conta...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06866.x

    authors: Mikami T,Horikawa T,Murakami T,Matsumoto T,Yamakawa A,Murayama S,Katagiri S,Shinagawa K,Suzuki M

    更新日期:1994-06-01 00:00:00

  • Bacterial triterpenoids of the hopane series as biomarkers for the chemotaxonomy of Burkholderia, Pseudomonas and Ralstonia spp.

    abstract::Hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus Pseudomonas. Whereas all strains related to Pseudomonas and Ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the Burkholderia species and in related soil isolates, which contained as m...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08974.x

    authors: Cvejic JH,Putra SR,El-Beltagy A,Hattori R,Hattori T,Rohmer M

    更新日期:2000-02-15 00:00:00

  • A new root-nodulating symbiont of the tropical legume Sesbania, Rhizobium sp. SIN-1, is closely related to R. galegae, a species that nodulates temperate legumes.

    abstract::Rhizobium sp. SIN-1, isolated in India from root nodules on the tropical legume Sesbania aculeata, also induces nitrogen-fixing nodules on roots of S. macrocarpa, S. speciosa, S. procumbens, S. punicea, S. rostrata, and Vigna unguiculata. Unlike Azorhizobium caulinodans, SIN-1 does not induce stem nodules on S. rostra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07908.x

    authors: Rana D,Krishnan HB

    更新日期:1995-12-01 00:00:00

  • Concomitant expression of E. coli cytosine deaminase and uracil phosphoribosyltransferase improves the cytotoxicity of 5-fluorocytosine.

    abstract::The prodrug activation system formed by the E. coli codA gene encoding cytosine deaminase (CD) and 5-fluorocytosine (5-FC) developed for selective cancer chemotherapy suffers from a sensitivity limitation in many tumour cells. In an attempt to improve the CD/5-FC suicide association, we combined the E. coli upp gene e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13205.x

    authors: Tiraby M,Cazaux C,Baron M,Drocourt D,Reynes JP,Tiraby G

    更新日期:1998-10-01 00:00:00

  • Regulation of the yeast vacuolar aminopeptidase Y gene (APY1) expression.

    abstract::The steady-state levels of the aminopeptidase Y (APY1) transcript and the level of assayable aminopeptidase Y activity were measured under a variety of nutritional conditions. Specific APY1 mRNA is less abundant in cells grown in minimal medium than in cells grown in rich medium, while active enzyme levels follow just...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08191.x

    authors: Herrera-Camacho I,Suárez-Rendueles P

    更新日期:1996-06-01 00:00:00

  • Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes.

    abstract::In this study, we were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis with subpopulations of porcine white blood cells (WBC). The mutants included those with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and a mutant with five major pathogenicity islands remove...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02305.x

    authors: Stepanova H,Volf J,Malcova M,Matiasovic J,Faldyna M,Rychlik I

    更新日期:2011-08-01 00:00:00

  • PCR primers specific for the genus Tuber reveal the presence of several truffle species in a truffle-ground.

    abstract::Truffles are hypogeous Ascomycete fungi belonging to the genus Tuber and forming fruiting bodies highly prized for their taste and aroma. The identification of the genus Tuber and its species is important to investigate their ecology and avoid fraud in the food market. As genus-specific primers are not available, the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01655.x

    authors: Zampieri E,Mello A,Bonfante P,Murat C

    更新日期:2009-08-01 00:00:00

  • Deacetoxycephalosporin C synthase isozymes exhibit diverse catalytic activity and substrate specificity.

    abstract::The biosynthesis of cephalosporins involving a thiozolidine ring expansion is catalyzed by deacetoxycephalosporin C synthase (DAOCS). In this study, three DAOCS isozymes were cloned and expressed as active enzymes together with Streptomyces jumonjinensis DAOCS that was newly isolated and partially characterized. The e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2003.tb11525.x

    authors: Chin HS,Sim J,Seah KI,Sim TS

    更新日期:2003-01-28 00:00:00

  • Proteomics and the stressful life of lactobacilli.

    abstract::Lactobacilli occupy specific ecological niches, where they represent a major component of foods, human and animal microbial communities. Employing these bacteria in industrial fermentations or for human health benefits exposes them to certain life-threatening conditions where their ability to adapt plays a key role in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/1574-6968.12274

    authors: Hussain MA,Hosseini Nezhad M,Sheng Y,Amoafo O

    更新日期:2013-12-01 00:00:00

  • The pWW0 plasmid imposes a stochastic expression regime to the chromosomal ortho pathway for benzoate metabolism in Pseudomonas putida.

    abstract::Environmental plasmids often expand the metabolic repertoire of bacteria that carry them, but they also interfere with the biochemical and genetic network of the host. The pWW0 plasmid born by Pseudomonas putida mt-2 encodes the TOL pathway for degradation of toluene/m-xylene through production of intermediate compoun...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12400

    authors: Silva-Rocha R,de Lorenzo V

    更新日期:2014-07-01 00:00:00

  • Human brain endothelial ATP synthase beta-subunit is mannose-insensitive binding target of FimH.

    abstract::Binding of meningitis-causing Escherichia coli K1 to human brain microvascular endothelial cells (HBMEC) contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01878.x

    authors: Shin S,Kim KS

    更新日期:2010-02-01 00:00:00

  • Evidence for inter- and intraspecies biofilm formation variability among a small group of coagulase-negative staphylococci.

    abstract::Coagulase-negative staphylococci (CoNS) are common bacterial colonizers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS sp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv175

    authors: Oliveira F,Lima CA,Brás S,França Â,Cerca N

    更新日期:2015-10-01 00:00:00

  • Comparison of five rep-PCR genomic fingerprinting methods for differentiation of fecal Escherichia coli from humans, poultry and wild birds.

    abstract::The development of a methodology to identify the origin of fecal pollution is important both for assessing the degree of risk posed to public health and for developing strategies to mitigate the environmental loading of pathogens associated with waterborne disease transmission. Five rep-PCR genomic fingerprinting meth...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00948.x

    authors: Mohapatra BR,Broersma K,Mazumder A

    更新日期:2007-12-01 00:00:00

  • Increased expression and hotspot mutations of the multidrug efflux transporter, CDR1 in azole-resistant Candida albicans isolates from vaginitis patients.

    abstract::The aims of our research were to investigate the gene expression of the multidrug efflux transporter, CDR1 and the major drug facilitator superfamily transporter, MDR1 gene in azole drug-resistant Candida albicans and Candida glabrata clinical isolates recovered from vaginitis patients; and to identify hotspot mutatio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.06.036

    authors: Looi CY,D' Silva EC,Seow HF,Rosli R,Ng KP,Chong PP

    更新日期:2005-08-15 00:00:00

  • The emergence of colistin-resistant Escherichia coli in chicken meats in Nepal.

    abstract::The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz237

    authors: Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

    更新日期:2019-10-01 00:00:00

  • Antimicrobial resistance in mollicutes: known and newly emerging mechanisms.

    abstract::This review is devoted to the mechanisms of antibiotic resistance in mollicutes (class Bacilli, subclass Mollicutes), the smallest self-replicating bacteria, that can cause diseases in plants, animals and humans, and also contaminate cell cultures and vaccine preparations. Research in this area has been mainly based o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny185

    authors: Chernov VM,Chernova OA,Mouzykantov AA,Medvedeva ES,Baranova NB,Malygina TY,Aminov RI,Trushin MV

    更新日期:2018-09-01 00:00:00