Human brain endothelial ATP synthase beta-subunit is mannose-insensitive binding target of FimH.

Abstract:

:Binding of meningitis-causing Escherichia coli K1 to human brain microvascular endothelial cells (HBMEC) contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP synthase beta-subunit and actin proteins from the surface biotinylated HBMEC as the mannose-insensitive binding targets for FimH. Co-immunoprecipitation experiments in the presence of alpha-methyl mannose verified the binding of FimH to ATP synthase beta-subunit of HBMEC. ATP synthase beta-subunit antibody decreased E. coli K1 binding to HBMEC in the presence of alpha-methyl mannose. Taken together, these findings demonstrate that FimH of E. coli K1 binds to HBMEC in both mannose-sensitive and -insensitive manner.

journal_name

FEMS Microbiol Lett

authors

Shin S,Kim KS

doi

10.1111/j.1574-6968.2009.01878.x

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

156-62

issue

2

eissn

0378-1097

issn

1574-6968

pii

FML1878

journal_volume

303

pub_type

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