Concomitant expression of E. coli cytosine deaminase and uracil phosphoribosyltransferase improves the cytotoxicity of 5-fluorocytosine.

abstract：:PhoP was previously shown to be important for Yersinia pestis survival in macrophage and under macrophage-induced stresses. In this work, a phoP disruptant of Y. pestis 201 was generated using the Red cloning procedure. The transcription profile of the wild-type Y. pestis was then compared with that of the phoP mutant...

journal_title：FEMS microbiology letters

authors： Zhou D,Han Y,Qin L,Chen Z,Qiu J,Song Y,Li B,Wang J,Guo Z,Du Z,Wang X,Yang R

更新日期：2005-09-01 00:00:00

abstract：:Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by...

journal_title：FEMS microbiology letters

authors： Fang W,Luther DA,Oliver SP

更新日期：1998-09-15 00:00:00

abstract：:Coxiella is a genus of obligate intracellular bacteria engaged in a variety of interactions with eukaryotes. The type species, Coxiella burnetii, infects several vertebrate species, including humans, and is the causative agent of Q fever. Multiple copies of a specific transposable element, the insertion sequence IS111...

journal_title：FEMS microbiology letters

authors： Duron O

更新日期：2015-09-01 00:00:00

abstract：:The clonal relationship of thirty E. coli strains of 0 antigen serotype 06 isolated from human, dog, pig or cow infections were investigated. Two main clones with serotypes 06 : H1 or 06 : H31, H- were identified. Isolates from humans, dogs, pigs and cows were found in both clones, indicating that animals are a possib...

journal_title：FEMS microbiology letters

authors： Cherifi A,Contrepois M,Picard B,Goullet P,Orskov I,Orskov F,De Rycke J

更新日期：1991-05-15 00:00:00

abstract：:The genes encoding the desulforubidin type of dissimilatory sulfite reductase (Dsr) from the sulfate-reducing bacteria Desulfobacter vibrioformis and Desulfobulbus rhabdoformis were cloned and sequenced. Similar to the genes for dissimilatory sulfite reductase from the genera Archaeoglobus, Desulfovibrio and Desulfoto...

journal_title：FEMS microbiology letters

authors： Larsen,Lien T,Birkeland NK

更新日期：2000-05-01 00:00:00

abstract：:Mycoplasma fermentans has been reported to be pathogenic for man. All fourteen strains tested contain an insertion sequence-like element (ISLE) which may be present in multiple copies. To determine whether ISLE copies are similarly distributed in different strains of M. fermentans, restriction enzyme digest fragments ...

journal_title：FEMS microbiology letters

authors： Pitcher D,Hilbocus J

更新日期：1998-03-01 00:00:00

abstract：:During protein synthesis, elongation factor G (EFG) participates at the steps of translocation and ribosome recycling. Fusidic acid (FA) is a bacteriostatic antibiotic, which traps EFG on ribosomes, stalling them on mRNAs. How the bacterial susceptibility to FA is determined, and which of the two functions of EFG (tra...

journal_title：FEMS microbiology letters

authors： Ayyub SA,Lahry K,Dobriyal D,Mondal S,Varshney U

更新日期：2018-07-01 00:00:00

abstract：:The DnaK chaperone system is involved in various cellular processes such as the control of the folded and oligomeric state of proteins under stress and non-stress conditions. In this study we functionally characterised the homologues of the DnaK system from Clostridium acetobutylicum DnaK, DnaJ, GrpE and OrfA were het...

journal_title：FEMS microbiology letters

authors： Rüngeling E,Laufen T,Bahl H

更新日期：1999-01-01 00:00:00

abstract：:Changes in gene expression play a critical role in enhancing the ability of cyanobacteria to survive under cold conditions. In the present study, Spirulina platensis cultures were grown at the optimal growth temperature, in the light, before being transferred to dark conditions at 22 degrees C. Two dimensional-differe...

journal_title：FEMS microbiology letters

authors： Jeamton W,Mungpakdee S,Sirijuntarut M,Prommeenate P,Cheevadhanarak S,Tanticharoen M,Hongsthong A

更新日期：2008-04-01 00:00:00

abstract：:Testosterone and 4-androstene-3,17-dione (androstenedione) binding proteins were found in the cytosol of Cochliobolus lunatus, a filamentous fungus. The maximal androgen binding occurred by 120 min at 0 degrees C. Androgen binding proteins were degraded by protease treatment and at high temperature. The mean Kd determ...

journal_title：FEMS microbiology letters

authors： Kastelic-Suhadolc T,Lenasi H

更新日期：1993-03-15 00:00:00

abstract：:A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

journal_title：FEMS microbiology letters

authors： Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

更新日期：2008-03-01 00:00:00

abstract：:Cell-associated hemagglutination (cHA) activity with human erythrocytes was examined for 468 clinical and 71 environmental strains of Vibrio parahaemolyticus. Approximately 95% of the strains tested were cHA positive irrespective of source or Kanagawa phenomenon. 75% of clinical strains showed relatively strong mannos...

journal_title：FEMS microbiology letters

authors： Nagayama K,Oguchi T,Arita M,Honda T

更新日期：1994-07-01 00:00:00

abstract：:Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phy...

journal_title：FEMS microbiology letters

authors： Shima S,Yanagi M,Saiki H

更新日期：1994-06-01 00:00:00

abstract：:It has been widely reported that pH mediates cadmium toxicity to bacteria. We used a tripartite approach to investigate mechanisms by which pH affects cadmium toxicity that included analyses of: (1) growth kinetics, (2) global gene expression, and (3) cadmium speciation. Cadmium extended the lag phase at pH 7, but not...

journal_title：FEMS microbiology letters

authors： Worden CR,Kovac WK,Dorn LA,Sandrin TR

更新日期：2009-04-01 00:00:00

abstract：:Pseudomonas sp. TLC6-6.5-4 is a multiple metal resistant plant growth-promoting bacteria isolated from copper-contaminated lake sediments. In this study, a comprehensive analysis of genes involved in copper resistance was performed by generating a library of transposon (Tn5) mutants. Two copper-sensitive mutants with ...

journal_title：FEMS microbiology letters

authors： Li K,Pidatala RR,Ramakrishna W

更新日期：2012-10-01 00:00:00

abstract：:Methanobacterium barkeri was found to catalyze the reductive dehalogenation of trichlorofluoromethane (CFC-11), also known as FREON 11. Products detected were CHFCl2, CH2FCl, CO and fluoride. ...

journal_title：FEMS microbiology letters

authors： Krone UE,Thauer RK

更新日期：1992-01-01 00:00:00

abstract：:The Mfa1 protein of Porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between P. gingivalis and Streptococcus gordonii. We utilized a promoter-lacZ reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. Promoter acti...

journal_title：FEMS microbiology letters

authors： Park Y,James CE,Yoshimura F,Lamont RJ

更新日期：2006-09-01 00:00:00

abstract：:The microbial community structure of two biofilters used for the oxidation of methane and organic trace gases generated in landfills was analysed by phospholipid fatty acid composition. Community structure varied with biofilter depth, reflecting varying conditions of substrate supply as well as of organic carbon conte...

journal_title：FEMS microbiology letters

authors： Gebert J,Gröngröft A,Schloter M,Gattinger A

更新日期：2004-11-01 00:00:00

abstract：:A cyclodextrin glycosyltransferase (CGTase) gene of Brevibacillus brevis CD162 was cloned into Escherichia coli using pUC19 as a vector. Determination of the nucleotide sequence showed the presence of an open reading frame of 2079 bp encoding a polypeptide of 693 amino acid residues, composed of a 20-amino acid signal...

journal_title：FEMS microbiology letters

authors： Kim MH,Sohn CB,Oh TK

更新日期：1998-07-15 00:00:00

abstract：:Bacillus cereus is becoming one of the more important causes of food poisoning in the industrialised world. It produces one emetic toxin and three different enterotoxins. The emetic toxin is a ring-shaped structure of three repeats of four amino and/or oxy acids: [D-O-Leu-D-Ala-L-O-Val-L-Val]3. This ring structure has...

journal_title：FEMS microbiology letters

authors： Granum PE,Lund T

更新日期：1997-12-15 00:00:00

abstract：:The distribution of the alcohol dehydrogenase gene (adh) among different Archaea was investigated by Southern blot analysis revealing the potentiality of the adh gene as a specific marker for the genus Sulfolobus. Moreover, the in vivo expression of the adh gene from a new isolate of Sulfolobus solfataricus, G theta, ...

journal_title：FEMS microbiology letters

authors： Cannio R,Fiorentino G,Rossi M,Bartolucci S

更新日期：1999-01-01 00:00:00

abstract：:The binding and hemagglutinating activities of the B subunit(s) of the heat-labile enterotoxin (LTh-B) isolated from human enterotoxigenic Escherichia coli were investigated. The binding of 125I-labeled LTh-B to neuraminidase-treated human type B erythrocytes was most effectively inhibited by ganglioside GM1. A number...

journal_title：FEMS microbiology letters

authors： Sugii S,Tsuji T

更新日期：1990-01-01 00:00:00

abstract：:The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden-Meyerhof pathway in bacteria and eukarya. This archaeal glycolysis variant consists of several novel enzymes, some of which catalyz...

journal_title：FEMS microbiology letters

authors： van de Werken HJ,Verhees CH,Akerboom J,de Vos WM,van der Oost J

更新日期：2006-07-01 00:00:00

abstract：:We transformed haploid mycelium of Hebeloma cylindrosporum via Agrobacterium tumefaciens and optimised the procedure to develop a new tool for insertional mutagenesis in this fungus. Southern blot analysis of 83 randomly selected transformants showed that they all contained plasmid inserts. Each of them showed a uniqu...

journal_title：FEMS microbiology letters

authors： Combier JP,Melayah D,Raffier C,Gay G,Marmeisse R

更新日期：2003-03-14 00:00:00

abstract：:Bacteriophage M102 is a virulent siphophage that propagates in some serotype c Streptococcus mutans strains, but not in S. mutans of serotype e, f or k. The serotype of S. mutans is determined by the glucose side chain of rhamnose-glucose polysaccharide (RGP). Because the first step in the bacteriophage infection proc...

journal_title：FEMS microbiology letters

authors： Shibata Y,Yamashita Y,van der Ploeg JR

更新日期：2009-05-01 00:00:00

abstract：:Bacillus subtilis cells must have cytochromes for growth and can synthesize cytochromes of a-, b-, c-, d-, and o-types. After a long lag, our knowledge of the structure, genetics and specific role for these cytochromes is now growing exponentially as the result of recent research. This progress is reviewed here and in...

journal_title：FEMS microbiology letters

authors： von Wachenfeldt C,Hederstedt L

更新日期：1992-12-15 00:00:00

abstract：:This study provides a novel qRT-PCR protocol for specific detection and proof of viability of Phytophthora in environmental samples based on differential accumulation of cox II transcripts. Chemical and physical treatments were tested for their ability to induce in vitro the accumulation of cytochrome oxidase genes en...

journal_title：FEMS microbiology letters

authors： Vannini A,Tomassini A,Bruni N,Vettraino AM

更新日期：2014-04-01 00:00:00

abstract：:The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), cons...

journal_title：FEMS microbiology letters

authors： Lanfranco L,Wyss P,Marzachí C,Bonfante P

更新日期：1993-12-15 00:00:00

abstract：:Weissella cibaria isolated from human saliva produces a soluble glucan that predominantly has alpha-1,6-glucosidic type linkages. Using degenerated primers that were selected based on the amino acid sequences of conserved regions from known glucansucrases, a single 2.7-kb fragment was isolated. In subsequent steps, a ...

journal_title：FEMS microbiology letters

authors： Kang HK,Oh JS,Kim D

更新日期：2009-03-01 00:00:00

abstract：:Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and...

journal_title：FEMS microbiology letters

authors： Korycka-Machala M,Brzostek A,Rozalska S,Rumijowska-Galewicz A,Dziedzic R,Bowater R,Dziadek J

更新日期：2006-05-01 00:00:00