Distinct DNA repair pathways involving RecA and nonhomologous end joining in Mycobacterium smegmatis.

Abstract:

:Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and in their response to chemicals that damage DNA. The results provide in vivo evidence that only NHEJ is required for the repair of noncompatible DNA ends. By contrast, the response of mycobacteria to mitomycin C preferentially involved a RecA-dependent pathway.

journal_name

FEMS Microbiol Lett

authors

Korycka-Machala M,Brzostek A,Rozalska S,Rumijowska-Galewicz A,Dziedzic R,Bowater R,Dziadek J

doi

10.1111/j.1574-6968.2006.00199.x

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

83-91

issue

1

eissn

0378-1097

issn

1574-6968

pii

FML199

journal_volume

258

pub_type

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