Abstract:
:A chromosomal DNA fragment from Streptomyces antibioticus ATCC11891 was isolated by its ability to stimulate actinorhodin and undecylprodigiosin biosynthesis in Streptomyces lividans TK21. This fragment includes two open reading frames, whose deduced translated products resemble enzymes involved in sulfur metabolism (ORF1) and LysR-type transcriptional regulators (ORF2). The cloning of the promoter region of ORF2 (abaB) in high copy number led to overproduction of both antibiotics suggesting that this phenotype might well be due to titration by this region of one or more proteins. Southern blot analysis revealed that abaB gene is highly conserved among all streptomycetes tested.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Scheu AK,Martínez E,Soliveri J,Malpartida Fdoi
10.1111/j.1574-6968.1997.tb10216.xsubject
Has Abstractpub_date
1997-02-01 00:00:00pages
29-36issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(96)00498-3journal_volume
147pub_type
杂志文章abstract::Ralstonia eutropha H16 is a Gram-negative lithoautotrophic bacterium and is one of the best biopolymer-producing bacteria. It can grow to high cell densities either under lithoautotrophic or under heterotrophic conditions, which makes it suitable for a number of biotechnological applications. Also, R. eutropha H16 can...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2010.02041.x
更新日期:2010-08-01 00:00:00
abstract::Outer membrane-associated proteins of 18 kDa were expressed by enteroaggregative Escherichia coli (EAggEC) belonging to serotypes O126:H27 and O44:H18, which hybridized with a probe derived from a plasmid necessary for enteroaggregative adhesion. The 18 kDa proteins expressed by strains of E. coli, belonging to these ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1995.tb07804.x
更新日期:1995-10-01 00:00:00
abstract::We have observed significant cell lysis upon temperature up-shift of recombinant Escherichia coli cultures harboring CI857-repressed lambda-based expression vectors. This event, that becomes evident about 30-40 min after the heat shock, takes place when using the lambda promoter system in Ind- lysogenic strains, but n...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb13750.x
更新日期:1999-08-15 00:00:00
abstract::Acylated homoserine lactone (AHL)-based quorum sensing (QS) has been reported to be present only in Gram-negative microorganisms. Isolation of a novel Gram-positive microorganism from sea water, capable of producing AHL, is reported here. The isolate (GenBank: JF915892, designated as MPO) belonging to the Exiguobacter...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/1574-6968.12123
更新日期:2013-06-01 00:00:00
abstract::A family of stable lactococcal vectors have been constructed based on the pFX1 replicon using either the alpha fragment or the complete Escherichia coli lacZ gene as a selective marker. These vectors also incorporate multiple cloning sites and examples are given of their use for gene cloning and translational fusion s...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1991.tb04321.x
更新日期:1991-01-01 00:00:00
abstract::Plant protection via disease-suppressive bacteria in desert farming requires specific biological control agents (BCAs) adapted to the unique arid conditions. We performed an ecological study of below-ground communities in desert farm soil and untreated desert soil, and based on these findings, selected antagonists wer...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/1574-6968.12089
更新日期:2013-05-01 00:00:00
abstract::Thermophilic bacteria have recently attracted great attention because of their potential application in improving different biochemical processes such as anaerobic digestion of various substrates, wastewater treatment or hydrogen production. In this study we report on the design of a specific 16S rRNA-targeted oligonu...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/1574-6968.12528
更新日期:2014-09-01 00:00:00
abstract::Nocardia asteroides complex organisms derived from human specimens between 1979 and 1992 were identified to the species level. Of 117 N. asteroides complex organisms, 34 (29%) were N. farcinica, 28 (24%) were N. nova, and 55 (47%) were N. asteroides sensu stricto. An analysis of the specimen sites from which the organ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1993.tb06335.x
更新日期:1993-07-01 00:00:00
abstract::Chronological analysis of 125 Vibrio cholerae O139 strains isolated during 1993-2005 in Kolkata revealed the prevalence of two new genotypes of cholera toxin (CT) and novel combinations of ctxB and rstR alleles resulting in variant CTX prophages. One of the new genotypes of ctxB, which first appeared in 1996 with the ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2009.01856.x
更新日期:2010-02-01 00:00:00
abstract::The phyCg gene encoding a new phytase from Citrobacter gillenii was optimized, synthesized, cloned and expressed in Pichia pastoris. Analysis of the amino acid sequence of the enzyme showed that it belongs to the histidine acid phosphatase family. The amino acid sequence of the PhyCg phytase has the highest homology (...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnaa217
更新日期:2020-12-21 00:00:00
abstract::The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracel...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2001.tb10833.x
更新日期:2001-09-25 00:00:00
abstract::Sulfamate is an analogue of thiosulfate, and the sodium and potassium salts of sulfamic acid inhibited the chemolithoautotrophic growth on thiosulfate of Acidithiobacillus ferrooxidans and Halothiobacillus neapolitanus. The chemo-organotrophic growth of Paracoccus versutus on sucrose was similarly inhibited by sulfama...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2006.00437.x
更新日期:2006-11-01 00:00:00
abstract::During our screening of amylolytic microorganisms from Brazilian fruits, we isolated a yeast strain classified as Cryptococcus flavus. When grown on starch-containing medium this strain exhibited the highest amylase production after 24 h of cultivation. The extracellular amylase from C. flavus was purified from the cu...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00955-8
更新日期:2004-02-16 00:00:00
abstract::In this study, we show that berberine chloride (BBR) has antimicrobial activities against all 43 tested strains of Staphylococcus aureus, an important human and animal pathogen. However, the response mechanisms of S. aureus to BBR are still poorly understood. Affymetrix GeneChips were used to determine the global tran...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2007.01031.x
更新日期:2008-02-01 00:00:00
abstract::A plasmid has been constructed which is a bifunctional vector for Escherichia coli and for streptomycetes, containing the cos site of Streptomyces phage phi C31. It can be efficiently transduced into S. lividans 66 and into several other Streptomyces species. The nucleotide sequence of a 311-bp DNA fragment containing...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(91)90183-b
更新日期:1991-03-01 00:00:00
abstract::The beta-barrel outer membrane protease Pla from Yersinia pestis is an important virulence factor in plague and enables initiation of the bubonic plague. Pla is a multifunctional protease whose expression also enhances bacterial adherence to extracellular matrix. It has remained uncertain whether the increase in cellu...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2006.00382.x
更新日期:2006-09-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2008.01140.x
更新日期:2008-06-01 00:00:00
abstract::Environmental persistence of Mycobacterium tuberculosis is subject to speculation. However, the reality that infected postmortem tissues can be a danger to pathologists and embalmers has worrisome implications. A few experimental studies have demonstrated the organism's ability to withstand exposure to embalming fluid...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2008.01150.x
更新日期:2008-06-01 00:00:00
abstract::A hybridized DNA fragment was cloned as a 7.6-kb fragment from Streptomyces lavendulae KCCS0263 using a 1.9-kb SacI-XbaI DNA fragment from S. cellulosae as a probe. The latter fragment encoded a beta-lactamase which can bind blue dextran. The hybridized region was reduced to a 2.8-kb KpnI-BclI fragment and the nucleot...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1993.tb06473.x
更新日期:1993-09-15 00:00:00
abstract::Adenylate kinase isoenzymes localised in the mitochondria and in the cytosol have been detected in extracts of glucose-grown Aspergillus nidulans using specific staining after electrophoresis on cellulose acetate. The isoenzymes have similar Km values for AMP, ADP and MgATP2- but may differ in the mechanism used for i...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(91)90146-2
更新日期:1991-03-01 00:00:00
abstract::The c-type cytochrome (OmcB) and the multicopper protein (OmpB) required for Fe(III) oxide reduction by Geobacter sulfurreducens were predicted previously to be outer membrane proteins, but it is not clear whether they are positioned in a manner that permits the interaction with Fe(III). Treatment of whole cells with ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2007.00915.x
更新日期:2007-12-01 00:00:00
abstract::A succinate semialdehyde dehydrogenase gene (gabD) was identified to be disrupted in a transposon-induced mutant of Ralstonia eutropha exhibiting the phenotype 4-hydroxybutyric acid-leaky. The native gabD gene was cloned by colony hybridization using a homologous gabD-specific DNA probe. DNA sequencing revealed an 145...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb08827.x
更新日期:1999-12-01 00:00:00
abstract::When bacterial cells are subjected to stress, cellular and population characteristics can change significantly. Rapid acquisition of these properties of cells in their native physiological state is necessary in order to precisely interpret their survival modes. The multiparametric analytical capability of flow cytomet...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2008.01410.x
更新日期:2009-01-01 00:00:00
abstract::In Corynebacterium glutamicum the promoter of the araBAD Escherichia coli gene is positively regulated by both arabinose and the araC gene product, as it is the case in the natural host. If the L-arabinose inducer and an active araC gene are present, significant amounts of araBAD promoter expression take place in the ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb13558.x
更新日期:1999-05-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium is a food-borne pathogen and a leading cause of gastroenteritis in humans. Recently, we sequenced a phage-type DT108 strain (L945) and found reads with high similarity to both Salmonella typhi strain CT18 plasmid pHCM2 and bacteriophage SSU5. In this study, we completely sequenc...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnv044
更新日期:2015-04-01 00:00:00
abstract::Cell differentiation is one of the marks of multicellular organisms. Terminally specialised nitrogen-fixing cells, termed heterocysts, evolved in filamentous cyanobacteria more than 2 Gya. The development of their spacing pattern has been thoroughly investigated in model organisms such as Anabaena sp. PCC 7120. This p...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnx154
更新日期:2017-08-15 00:00:00
abstract::When challenged by stresses such as starvation, the soil bacterium Bacillus subtilis produces an endospore surrounded by a proteinaceous coat composed of >70 proteins that are organized into three main layers: an amorphous undercoat, lightly staining lamellar inner coat and electron-dense outer coat. This coat protect...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2009.01737.x
更新日期:2009-10-01 00:00:00
abstract::A total of 129 sRNA candidates were identified in Brucella abortus 2308 in our previous work, and one candidate with potential to regulate expression of hemH gene was further analyzed in this study. We found that the novel sRNA can inhibit the expression of hemH and called it BsrH (Brucella sRNA regulating HemH). The ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnu017
更新日期:2015-01-01 00:00:00
abstract::To analyze regulation of the vanadium-dependent nitrogenase of Azotobacter vinelandii, plasmids carrying vnfE-, vnfH-, or vnfD-lacZ fusions were transferred to Escherichia coli. These genes were expressed only if VnfA was present. Deletions of the vnfE upstream region were constructed and comparison of a region necess...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1996.tb07992.x
更新日期:1996-01-15 00:00:00
abstract::Macrolide is inactivated with ATP plus crude extract of Escherichia coli producing macrolide 2'-phosphotransferase (MPH(2')), but not by living cells. Therefore, a convenient method for detection of MPH(2') using intact cells is needed. In this report, we determine that the modified lysozyme-DNase-RNase (LDR) method (...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1998.tb13227.x
更新日期:1998-10-15 00:00:00