Detection of Nitrosomonas spp. by polymerase chain reaction.

Abstract:

:A unique genomic DNA fragment was isolated from Nitrosomonas europaea ATCC 19718. Based on the sequence of this fragment, oligonucleotide primers for polymerase chain reaction amplification were prepared which amplify sequences of 775 and 658 bp. The predicted DNA fragments were both amplified from the genome of N. europaea and a Nitrosomonas spp. isolated from a local oxidation pond. The primers failed to amplify DNA from the genomes of the ammonia oxidiser Nitrosolobous multiformis, the nitrite oxidiser Nitrococcus mobilis as well as from the genomes of other unrelated heterotrophic bacteria. These DNA sequences could be amplified from 0.01 ng of N. europaea genomic DNA or from 100 intact cells, and it was possible to detect Nitrosomonas DNA in a DNA mixture extracted from water samples drawn from a local oxidation pond.

journal_name

FEMS Microbiol Lett

authors

Nejidat A,Abeliovich A

doi

10.1111/j.1574-6968.1994.tb07029.x

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

191-4

issue

1-2

eissn

0378-1097

issn

1574-6968

journal_volume

120

pub_type

杂志文章
  • Impact of land use history on the arbuscular mycorrhizal fungal diversity in arid soils of Argentinean farming fields.

    abstract::Arbuscular mycorrhizal fungi (AMF) are a key soil functional group, with an important potential to increase crop productivity and sustainable agriculture including food security. However, there is clear evidence that land uses, crop rotations and soil features affect the AMF diversity and their community functioning i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa114

    authors: Ontivero RE,Voyron S,Allione LVR,Bianco P,Bianciotto V,Iriarte HJ,Lugo MA,Lumini E

    更新日期:2020-07-01 00:00:00

  • Test parameters and cell chain length of Streptococcus thermophilus affect the microbial adhesion to hydrocarbons assay: a methodical approach.

    abstract::The microbial adhesion to hydrocarbons (MATH) test is one of the most common method to determine the hydrophobicity of cell surfaces. Despite its prevalence, no standard test parameters are used in literature, making a direct comparison of data almost impossible. Criticism also focuses on test parameters that may mask...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz150

    authors: Nachtigall C,Weber C,Rothenburger S,Jaros D,Rohm H

    更新日期:2019-06-01 00:00:00

  • Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker.

    abstract::The sacB gene of Bacillus subtilis was successfully applied in various Arthrobacter, Brevibacterium, Corynebacterium and Rhodococcus strains for the isolation of transposable elements. Three different insertion sequence (IS) elements entrapped in sacB were isolated. The IS elements IS-Bl and IS-Cg isolated from Brevib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07381.x

    authors: Jäger W,Schäfer A,Kalinowski J,Pühler A

    更新日期:1995-02-01 00:00:00

  • Genetic evidence that hepA gene is involved in the normal deposition of the envelope of both heterocysts and akinetes in Anabaena variabilis ATCC 29413.

    abstract::The hepA gene in Anabaena sp. strain PCC 7120 is required for normal formation of the polysaccharide layer of the heterocyst envelope. A plasmid bearing hepA, interrupted by a neomycin-resistance cassette, was transferred by conjugation to wild type Anabaena variabilis ATCC 29413, so that the interrupted hepA gene rep...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07202.x

    authors: Leganés F

    更新日期:1994-10-15 00:00:00

  • Lactococcus piscium sp. nov. a new Lactococcus species from salmonid fish.

    abstract::Chemical and molecular taxonomic studies were performed on a representative strain of some lactic acid bacteria of unknown taxonomic position isolated from salmonid fish. The results demonstrate that the fish bacterium represents a new species of the genus Lactococcus for which the name Lactococcus piscium sp. nov. is...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90134-c

    authors: Williams AM,Fryer JL,Collins MD

    更新日期:1990-03-01 00:00:00

  • Beyond the single answer: a process-oriented exam for science students.

    abstract::This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw176

    authors: Nastos S,Rangachari PK

    更新日期:2016-08-01 00:00:00

  • Construction of improved plasmid vectors for promoter characterization in Pseudomonas aeruginosa and other gram-negative bacteria.

    abstract::We report the construction of two broad host range promoter-probe plasmid vectors for rapid analysis of promoters in Gram-negative bacteria. The new vectors, pME4507 and pME4510, carry carbenicillin and gentamycin resistance genes, respectively, and are small sized (4 kb) with a flexible multiple cloning site to facil...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13315.x

    authors: Rist M,Kertesz MA

    更新日期:1998-12-01 00:00:00

  • Ergosterol levels in two L-methionine-enriched mutants of the methylotrophic yeast Candida boidinii ICCF26.

    abstract::Two L-methionine-enriched mutants, SN-78 and SE-57, were isolated in a sulphur-deficient medium from the methylotrophic yeast Candida boidinii ICCF26. No significant differences were detected between the L-cysteine pools of the mutants and the wild-type. In mutant strain SE-57, S-adenosylmethionine and ergosterol leve...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90144-d

    authors: Avram D,Stan R

    更新日期:1992-11-01 00:00:00

  • Correlation between glycosylation of flagellin proteins and sensitivity of flagellar filaments to Triton X-100 in methanogens.

    abstract::The flagellins of Methanospirillum hungatei strains JF1 and GP1, Methanococcus deltae, and Methanothermus fervidus are glycosylated. Isolated flagellar filaments from these organisms are dissociated by low concentrations (0.5% (v/v)) of Triton X-100. Flagellar filaments from other methanogens (Methanococcus voltae, Me...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90616-v

    authors: Faguy DM,Koval SF,Jarrell KF

    更新日期:1992-01-01 00:00:00

  • SfbII protein, a fibronectin binding surface protein of group A streptococci, is a serum opacity factor with high serotype-specific apolipoproteinase activity.

    abstract::Serum opacity factor (SOF) is produced by group A streptococci belonging to certain M types. SOF cleaves the apolipoprotein component of the high density lipoprotein fraction of serum rendering it insoluble which in turn leads to serum opacity. SfbII protein, a fibronectin binding surface protein cloned from group A s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb08692.x

    authors: Kreikemeyer B,Martin DR,Chhatwal GS

    更新日期:1999-09-15 00:00:00

  • Expression of the mphB gene for macrolide 2'-phosphotransferase II from Escherichia coli in Staphylococcus aureus.

    abstract::The genes mphA and mphB encode macrolide 2'-phosphotransferases I and II, respectively, and they confer resistance to macrolide antibiotics in Escherichia coli. To study the expression of these genes in Gram-positive bacteria, we constructed recombinant plasmids that consisted of an mph gene and the pUB110 vector in B...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12880.x

    authors: Noguchi N,Tamura Y,Katayama J,Narui K

    更新日期:1998-02-15 00:00:00

  • Helicobacter pylori HopQ outer membrane protein attenuates bacterial adherence to gastric epithelial cells.

    abstract::Helicobacter pylori genomes contain about 30 hop genes that encode outer membrane proteins. Helicobacter pylori hopQ alleles exhibit a high level of genetic diversity, and two families of hopQ alleles have been described. Type I hopQ alleles are found more commonly in cag-positive H. pylori strains from patients with ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01368.x

    authors: Loh JT,Torres VJ,Algood HM,McClain MS,Cover TL

    更新日期:2008-12-01 00:00:00

  • Cloning and analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp.: evidence that the gene is both plasmid and chromosomally located.

    abstract::The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06817.x

    authors: Schembri MA,Bayly RC,Davies JK

    更新日期:1994-05-01 00:00:00

  • Novel putative rhizobial species with different symbiovars nodulate Lotus creticus and their differential preference to distinctive soil properties.

    abstract::Phylogenetically diverse rhizobial strains endemic to Tunisia were isolated from symbiotic nodules of Lotus creticus, growing in different arid extremophile geographical regions of Tunisia, and speciated using multiloci-phylogenetic analysis as Neorhizobium huautlense (LCK33, LCK35, LCO42 and LCO49), Ensifer numidicus...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa084

    authors: Rejili M,BenAbderrahim MA,Mars M,Sherrier JD

    更新日期:2020-06-01 00:00:00

  • Membrane proteins with molecular masses of 88, 90 and 150 kDa are responsible for binding of human immunoglobulin G Fc fragment to the native cells of Mycoplasma salivarium.

    abstract::Mycoplasma salivarium cells bound the Fc fragment of human immunoglobulin G. The activity was remarkably enhanced by Mn2+, but not by Mg2+ and Ca2+; significantly inhibited by D-mannose; and reduced by pronase treatment of the cells. About 90% of the cells treated with pronase were not disrupted. Sodium dodecyl sulfat...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07239.x

    authors: Shibata K,Sawa Y,Inoue S,Noda M,Watanabe T

    更新日期:1994-11-01 00:00:00

  • Investigation of direct vs. indirect involvement of the c-type cytochrome MacA in Fe(III) reduction by Geobacter sulfurreducens.

    abstract::The electron transfer pathway to Fe(III) reduction in Geobacter sulfurreducens has been hypothesized to consist of a series of c-type cytochromes. Previous genetic studies suggested that the inner membrane-associated, c-type cytochrome, MacA, was a component of the electron transfer chain leading to Fe(III) reduction ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01252.x

    authors: Kim BC,Lovley DR

    更新日期:2008-09-01 00:00:00

  • Comparison of the effect of salinity on the D/H ratio of fatty acids of heterotrophic and photoautotrophic microorganisms.

    abstract::The core metabolism of microorganisms has a major influence on the hydrogen isotopic composition of their fatty acids. Heterotrophic microorganisms produce fatty acids with a deuterium to hydrogen (D/H) ratio either slightly depleted or enriched in D compared to the growth water, while photo- and chemoautotrophic micr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv065

    authors: Heinzelmann SM,Chivall D,M'Boule D,Sinke-Schoen D,Villanueva L,Damsté JS,Schouten S,van der Meer MT

    更新日期:2015-05-01 00:00:00

  • Production of novel pullulanases at high concentrations by two newly isolated thermophilic clostridia.

    abstract::Two thermophilic bacteria, which are capable of growing on starch at 60-70 degrees C under anaerobic conditions, were isolated from a sugar refinery in Uelzen and from Solar lake in Israel. On the basis of their physiological characteristics they were identified as Clostridium thermohydrosulfuricum Uel 1 and C. thermo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90429-t

    authors: Klingeberg M,Hippe H,Antranikian G

    更新日期:1990-05-01 00:00:00

  • Nucleotide sequence and analysis of the gene in Borrelia burgdorferi encoding the immunogenic P39 antigen.

    abstract::The P39 antigen is a specific, highly conserved, and immunogenic protein of Lyme disease spirochetes, Borrelia burgdorferi sensu lato. The nucleotide sequence of the gene encoding this protein was determined and found to be the first of two tandemly arranged open reading frames located on the spirochete's chromosome. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06917.x

    authors: Simpson WJ,Cieplak W,Schrumpf ME,Barbour AG,Schwan TG

    更新日期:1994-06-15 00:00:00

  • PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces.

    abstract::Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10599.x

    authors: Mikosza AS,La T,Margawani KR,Brooke CJ,Hampson DJ

    更新日期:2001-04-13 00:00:00

  • Lipid modification of prelipoproteins is dispensable for growth in vitro but essential for virulence in Streptococcus pneumoniae.

    abstract::A Deltalgt (Lgt, lipoprotein diacylglyceryl transferase) isogenic mutant was obtained which indicates that lgt is not essential for cell growth in vitro, like in the Gram-positive bacterium Bacillus subtilis, but unlike in the proteobacteria Escherichia coli and Salmonella typhimurium. The mutation was transduced to a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10720.x

    authors: Petit CM,Brown JR,Ingraham K,Bryant AP,Holmes DJ

    更新日期:2001-06-25 00:00:00

  • Far different levels of gene expression provided by an oriented cloning system in Bacillus subtilis and Escherichia coli.

    abstract::A gene expression system for both Bacillus subtilis and Escherichia coli was developed. The expression vector, pHASH102, produces any combination of promoter and open reading frame to be expressed based on the T-extended cloning method. Because the pHASH series vectors are designed to shuttle between the genome and a ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00171-X

    authors: Ohashi Y,Ohshima H,Tsuge K,Itaya M

    更新日期:2003-04-11 00:00:00

  • Mycobacterium avium rough-to-smooth colony conversion resulting from growth in Tween 80 without presence of type-specific glycopeptidolipid antigens.

    abstract::Growth of a Mycobacterium avium complex serotype 20 rough-colony variant in 1.0% Tween 80 resulted in a smooth-colony morphological conversion that was reversible upon removal of Tween and was not associated with the presence of serotype-specific glycopeptidolipid antigens. Electron microscopic examination suggested t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90158-k

    authors: Wright EL,Pourshafie M,Barrow WW

    更新日期:1992-11-01 00:00:00

  • The distribution of insertion sequences in the genome of Shigella flexneri strain 2457T.

    abstract::Shigella flexneri, which causes shigellosis in humans, evolved from Escherichia coli. The sequencing of Shigella genomes has revealed that a large number of insertion sequence (IS) elements (over 200 elements) reside in the genome. Although the presence of these elements has been noted previously and summarized, more ...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/j.1574-6968.2007.00957.x

    authors: Zaghloul L,Tang C,Chin HY,Bek EJ,Lan R,Tanaka MM

    更新日期:2007-12-01 00:00:00

  • A novel colorimetric method for the detection of Escherichia coli using cytochrome c peroxidase-encoding bacteriophage.

    abstract::A new rapid and simple method was developed for the detection of Escherichia coli by constructing a recombinant T4 phage carrying the cytochrome c peroxidase gene derived from Saccharomyces cerevisiae (T4ccp) using which, the colorimetric detection of E. coli K12 was examined. The oxidation activity toward the chromog...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12382

    authors: Hoang HA,Abe M,Nakasaki K

    更新日期:2014-03-01 00:00:00

  • Glucomannan utilization operon of Bacillus subtilis.

    abstract::This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong ind...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.01018.x

    authors: Sadaie Y,Nakadate H,Fukui R,Yee LM,Asai K

    更新日期:2008-02-01 00:00:00

  • Proteomics and the stressful life of lactobacilli.

    abstract::Lactobacilli occupy specific ecological niches, where they represent a major component of foods, human and animal microbial communities. Employing these bacteria in industrial fermentations or for human health benefits exposes them to certain life-threatening conditions where their ability to adapt plays a key role in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/1574-6968.12274

    authors: Hussain MA,Hosseini Nezhad M,Sheng Y,Amoafo O

    更新日期:2013-12-01 00:00:00

  • Genetic engineering of Streptococcus gordonii for the simultaneous display of two heterologous proteins at the bacterial surface.

    abstract::The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11172.x

    authors: Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

    更新日期:2002-04-23 00:00:00

  • Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium.

    abstract::The RcsCDB (Rcs) phosphorelay system regulates capsule synthesis, flagella production and other cellular activities in several enteric bacteria. This system consists of three proteins: the sensor RcsC, the cognate response regulator RcsB and the histidine-containing phosphotransfer protein RcsD (YojN), which is hypoth...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01771.x

    authors: Pescaretti Mde L,Morero R,Delgado MA

    更新日期:2009-11-01 00:00:00

  • Characterization of the protein processing and secretion pathways in a comprehensive set of expressed sequence tags from Trichoderma reesei.

    abstract::Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein fo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00916-9

    authors: Diener SE,Dunn-Coleman N,Foreman P,Houfek TD,Teunissen PJ,van Solingen P,Dankmeyer L,Mitchell TK,Ward M,Dean RA

    更新日期:2004-01-30 00:00:00