Novel putative rhizobial species with different symbiovars nodulate Lotus creticus and their differential preference to distinctive soil properties.

abstract：:In this study we have investigated the ability of nonencapsulated, nontypeable Haemophilus influenzae, NT477 to survive in the J774 mouse macrophage-like cell line. Viable, intracellular nontypeable H. influenzae could still be recovered from macrophages 72 h after phagocytosis. In contrast, H. influenzae strain Rd, a...

journal_title：FEMS microbiology letters

authors： Craig JE,Cliffe A,Garnett K,High NJ

更新日期：2001-09-11 00:00:00

abstract：:Two bifunctional plasmid vectors pZG5 (7.45 kb) and pZG6 (6.95 kb), for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of the multicopy broad-host-range Streptomyces plasmid pIJ350 with E. coli plasmids Bluescribe M13- (pZG5) or pUC18 (pZG6). Both plasmids possess several ...

journal_title：FEMS microbiology letters

authors： Durajlija S,Pigac J,Gamulin V

更新日期：1991-10-15 00:00:00

abstract：:A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cl...

journal_title：FEMS microbiology letters

authors： Palmer HM,Powell NB,Ala'Aldeen DA,Wilton J,Borriello SP

更新日期：1993-06-15 00:00:00

abstract：:The spirochaete, Serpulina pilosicoli, is the agent of intestinal spirochaetosis, a diarrhoeal disease of humans and other species. By mechanisms as yet unknown, large numbers of these spirochaetes intimately attach to the colonic mucosa by one cell end. In some infected individuals, the spirochaetes may invade the la...

journal_title：FEMS microbiology letters

authors： Hartland EL,Mikosza AS,Robins-Browne RM,Hampson DJ

更新日期：1998-08-01 00:00:00

abstract：:The genus Pycnoporus forms a group of four species known especially for producing high redox potential laccases suitable for white biotechnology. A sample of 36 Pycnoporus strains originating from different geographical areas was studied to seek informative molecular markers for the typing of new strains in laboratory...

journal_title：FEMS microbiology letters

authors： Lesage-Meessen L,Haon M,Uzan E,Levasseur A,Piumi F,Navarro D,Taussac S,Favel A,Lomascolo A

更新日期：2011-12-01 00:00:00

abstract：:We developed a multiplex PCR to detect the presence of methicillin- (mecA), cadmium/zinc-(czrC) and antiseptic-resistant (qacA/B) staphylococci and to identify Panton-Valentine leukocidin (PVL)-positive and -negative Staphylococcus aureus and coagulase-negative staphylococci (CoNS) from infected and healthy eyes. The ...

journal_title：FEMS microbiology letters

authors： Panda S,Kar S,Choudhury R,Sharma S,Singh DV

更新日期：2014-03-01 00:00:00

abstract：:The stable carbon isotopic compositions of the inorganic carbon source, bulk cell material, and isoprenoid lipids of the hyperthermophilic crenarchaeon Metallosphaera sedula, which uses a 3-hydroxypropionate-like pathway for autotrophic carbon fixation, have been measured. Bulk cell material was approximately 3 per th...

journal_title：FEMS microbiology letters

authors： van der Meer MT,Schouten S,Rijpstra WI,Fuchs G,Sinninghe Damsté JS

更新日期：2001-03-01 00:00:00

abstract：:A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...

journal_title：FEMS microbiology letters

authors： Law C,Kavanagh K,Whittaker P

更新日期：1994-01-01 00:00:00

abstract：:Two actinomycete strains, CHR3 and CHR28, were isolated from metal-contaminated sediments from Baltimore Inner Harbor. The isolates were classified as Streptomyces spp. Agar diffusion assays showed both isolates to be resistant to mercuric chloride and phenylmercuric acetate. Hybridization experiments indicated that g...

journal_title：FEMS microbiology letters

authors： Ravel J,Amoroso MJ,Colwell RR,Hill RT

更新日期：1998-05-01 00:00:00

abstract：:Phylogenetic relationships between Tuber pseudoexcavatum and other Tuber species were investigated by studying the sequences of four genes: 5.8S-ITS2, beta-tubulin, protein kinase C and elongation factor 1alpha. The four phylogenetic trees allowed to differentiate the black truffle clade, composed of two subclades, on...

journal_title：FEMS microbiology letters

authors： Wang Y,Tan ZM,Zhang DC,Murat C,Jeandroz S,Le Tacon F

更新日期：2006-06-01 00:00:00

abstract：:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

journal_title：FEMS microbiology letters

authors： Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

更新日期：1997-01-15 00:00:00

abstract：:The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning and sequencing of t...

journal_title：FEMS microbiology letters

authors： Kling JJ,Wright RL,Moncrief JS,Wilkins TD

更新日期：1997-01-15 00:00:00

abstract：:Ruminococcus albus is a Gram-positive bacterium that degrades plant cell walls in the rumen of herbivores. It was described to synthesize two major glycoside-hydrolases (Cel9B and Cel48A), which are exported and anchored at the cell surface. In bacteria, proteins destined to cross the cytoplasmic membrane are synthesi...

journal_title：FEMS microbiology letters

authors： Esbelin J,Martin C,Forano E,Mosoni P

更新日期：2009-05-01 00:00:00

abstract：:Testosterone and 4-androstene-3,17-dione (androstenedione) binding proteins were found in the cytosol of Cochliobolus lunatus, a filamentous fungus. The maximal androgen binding occurred by 120 min at 0 degrees C. Androgen binding proteins were degraded by protease treatment and at high temperature. The mean Kd determ...

journal_title：FEMS microbiology letters

authors： Kastelic-Suhadolc T,Lenasi H

更新日期：1993-03-15 00:00:00

abstract：:This study reports the production of astaxanthin in the photosynthetic bacterium Rhodovulum sulfidophilum, which has adequate precursor pools and storage capabilities for heterologous carotenoid production. Chemical mutagenesis was carried out using ethylmethane sulfonate to produce mutants with a modified carotenoid ...

journal_title：FEMS microbiology letters

authors： Mukoyama D,Takeyama H,Kondo Y,Matsunaga T

更新日期：2006-12-01 00:00:00

abstract：:The production of exopolysaccharides (EPSs) by a mucoid clinical isolate of Burkholderia cepacia involved in infections in cystic fibrosis patients, was studied. Depending on the growth conditions, this strain was able to produce two different EPS, namely PS-I and PS-II, either alone or together. PS-I is composed of e...

journal_title：FEMS microbiology letters

authors： Cérantola S,Montrozier H

更新日期：2001-08-07 00:00:00

abstract：:In the dental caries pathogen Streptococcus mutans, an MarR-like transcriptional regulator (RcrR), two ABC efflux pumps (RcrPQ) and two effector peptides encoded in the rcrRPQ operon provide molecular connections between stress tolerance, (p)ppGpp metabolism and genetic competence. Here, we examined the role of RcrRPQ...

journal_title：FEMS microbiology letters

authors： Shields RC,Burne RA

更新日期：2015-08-01 00:00:00

abstract：:For the dissemination of microbiology and science in general, social media is an underutilized but increasingly powerful tool, and one that transcends traditional avenues for scientific outputs. In order to reach and potentially inspire a deeply interested and general public, scientists should engage with platforms su...

journal_title：FEMS microbiology letters

authors： Hines HN

更新日期：2019-08-01 00:00:00

abstract：:The marine foodborne enteropathogen, Vibrio parahaemolyticus, has four putative catalase genes. Function of the katG-homologous genes, katG1(VPA0768) and katG2(VPA0453), was examined using gene deletion mutants, and compared with those of the katE-homologous genes, katE1(VPA1418) and katE2(VPA0305). Bacterial growth o...

journal_title：FEMS microbiology letters

authors： Yu SC,Fen SY,Chien CL,Wong HC

更新日期：2016-03-01 00:00:00

abstract：:A two-step enzymatic synthesis process of 4-hydroxyisoleucine is suggested. In the first step, the aldol condensation of acetaldehyde and alpha-ketobutyrate catalyzed by specific aldolase results in the formation of 4-hydroxy-3-methyl-2-keto-pentanoate (HMKP). In the second step, amination of HMKP by the branched-chai...

journal_title：FEMS microbiology letters

authors： Smirnov SV,Samsonova NN,Novikova AE,Matrosov NG,Rushkevich NY,Kodera T,Ogawa J,Yamanaka H,Shimizu S

更新日期：2007-08-01 00:00:00

abstract：:E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium L...

journal_title：FEMS microbiology letters

authors： Cortes-Perez NG,Bermúdez-Humarán LG,Le Loir Y,Rodriguez-Padilla C,Gruss A,Saucedo-Cárdenas O,Langella P,Montes-de-Oca-Luna R

更新日期：2003-12-05 00:00:00

abstract：:Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MA...

journal_title：FEMS microbiology letters

authors： Balážová T,Makovcová J,Šedo O,Slaný M,Faldyna M,Zdráhal Z

更新日期：2014-04-01 00:00:00

abstract：:A cloning cassette, carrying a modified amino-glycoside resistance gene (neo) from transposon Tn5 was constructed. Three restriction sites internal to the neo gene were eliminated by in vitro mutagenesis, allowing their use in designing new cloning vectors. The original, suboptimal transcription promoter was replaced ...

journal_title：FEMS microbiology letters

authors： Denis F,Brzezinski R

更新日期：1991-07-01 00:00:00

abstract：:Molecular information suggests that there is a broad diversity of acetogens in the rumen, distinct from any currently isolated acetogens. We combined molecular analysis with enrichment culture techniques to investigate this diversity further. Methane-inhibited, hydrogenotrophic enrichment cultures produced acetate as ...

journal_title：FEMS microbiology letters

authors： Gagen EJ,Padmanabha J,Denman SE,McSweeney CS

更新日期：2015-07-01 00:00:00

abstract：:Viable counts and activities of sulfate-reducing bacteria were determined in the oral cavities of 12 healthy volunteers. Of these, 10 harboured viable sulfate-reducing bacteria populations. Six separate sites were sampled: the posterior tongue, anterior tongue, mid buccal mucosa, vestibular mucosa, supragingival plaqu...

journal_title：FEMS microbiology letters

authors： Willis CL,Gibson GR,Allison C,Macfarlane S,Holt JS

更新日期：1995-06-15 00:00:00

abstract：:Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

journal_title：FEMS microbiology letters

authors： de Melo EJ,Mayerhoffer RO,de Souza W

更新日期：2000-04-01 00:00:00

abstract：:Burkholderia pseudomallei, a Gram-negative bacterium is found in the soil and water, mainly in Southeast Asia and Northern Australia. It is responsible for melioidosis in human and animals. The bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. The is...

journal_title：FEMS microbiology letters

authors： Lee MA,Liu Y

更新日期：2000-11-01 00:00:00

abstract：:The viability of probiotics is affected by several factors during manufacturing, storage and gastrointestinal tract passage. Protecting the probiotics from harmful conditions is particularly critical for oxygen sensitive species like Akkermansia muciniphila, a bacterium which recently has been proposed as a next-gener...

journal_title：FEMS microbiology letters

authors： Marcial-Coba MS,Saaby L,Knøchel S,Nielsen DS

更新日期：2019-01-01 00:00:00

abstract：:The devR-devS two-component system of Mycobacterium tuberculosis was identified earlier and partially characterized in our laboratory. A devR::kan mutant of M. tuberculosis was constructed by allelic exchange. The devR mutant strain showed reduced cell-to-cell adherence in comparison to the parental strain in laborato...

journal_title：FEMS microbiology letters

authors： Malhotra V,Sharma D,Ramanathan VD,Shakila H,Saini DK,Chakravorty S,Das TK,Li Q,Silver RF,Narayanan PR,Tyagi JS

更新日期：2004-02-16 00:00:00

abstract：:Weissella cibaria isolated from human saliva produces a soluble glucan that predominantly has alpha-1,6-glucosidic type linkages. Using degenerated primers that were selected based on the amino acid sequences of conserved regions from known glucansucrases, a single 2.7-kb fragment was isolated. In subsequent steps, a ...

journal_title：FEMS microbiology letters

authors： Kang HK,Oh JS,Kim D

更新日期：2009-03-01 00:00:00