Glucomannan utilization operon of Bacillus subtilis.


:This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong inducers of transcription. It is shown that an internal regulator gene (gmuR) encodes a repressor of the operon, as disruption of this gene enhances transcription of the operon in the absence of inducers. The expression of the glucomannan utilizing operon of B. subtilis is thus induced by degraded glucomannan products, and repressed by an internal repressor.


FEMS Microbiol Lett


Sadaie Y,Nakadate H,Fukui R,Yee LM,Asai K




Has Abstract


2008-02-01 00:00:00














  • Characterisation of integrons and antibiotic resistance genes in Danish multiresistant Salmonella enterica Typhimurium DT104.

    abstract::The presence and genetic content of integrons was investigated in eight Salmonella enteritica Typhimurium DT104 isolates from different pig herds in Denmark. Two different integrons were identified using PCR and sequencing. Each of the integrons carried a single resistance cassette in addition to the sull and qacE del...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sandvang D,Aarestrup FM,Jensen LB

    更新日期:1998-03-01 00:00:00

  • Genomic heterogeneity in Chlorobium limicola: chromosomic and plasmidic differences among strains.

    abstract::Chromosome analysis by pulsed-field gel electrophoresis shows a high level of genetic heterogeneity between the two subspecies of the green sulfur bacteria Chlorobium limicola analysed: C. limicola and C. limicola f. s. thiosulfatophilum. Currently, they are differentiated only by the ability to utilize thiosulfate as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Méndez-Alvarez S,Pavoń V,Esteve I,Guerrero R,Gaju N

    更新日期:1995-12-15 00:00:00

  • Phospholipids of Clostridium perfringens: a reexamination.

    abstract::We have identified phosphatidylethanolamine as one of the major phospholipids of Clostridium perfringens by two dimensional thin layer chromatography of the intact lipids and of their deacylation products and by liquid chromatography followed by mass spectrometry of the intact neutral phospholipid fraction. The princi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Johnston NC,Baker JK,Goldfine H

    更新日期:2004-04-01 00:00:00

  • Characterization of ISR region and development of a PCR assay for rapid detection of the fish pathogen Tenacibaculum soleae.

    abstract::The aims of this work were to characterize the 16S-23S internal spacer region of the fish pathogen Tenacibaculum soleae and to develop a PCR assay for its identification and detection. All T. soleae strains tested displayed a single internal spacer region class, containing tRNA(I) (le) and tRNA(A) ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: López JR,Hamman-Khalifa AM,Navas JI,de la Herran R

    更新日期:2011-11-01 00:00:00

  • Internal size variations in Tn1546-like elements due to the presence of IS1216V.

    abstract::In this study, internal size variations in the VanA gene cluster Tn1546, encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn1546, revealed that these variations were due to the presence of the IS sequenc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jensen LB

    更新日期:1998-12-15 00:00:00

  • Photorhabdus luminescens LN2 requires rpoS for nematicidal activity and nematode development.

    abstract::Photorhabdus (Enterobacteriaceae) bacteria are pathogenic to insects and mutualistic with entomopathogenic Heterorhabditis nematodes. Photorhabdus luminescens subsp. akhurstii LN2, associated with Heterorhabditis indica LN2, shows nematicidal activity against H. bacteriophora H06 infective juveniles (IJs). In the pres...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Qiu X,Wu C,Cao L,Ehlers RU,Han R

    更新日期:2016-03-01 00:00:00

  • Determination of viridans streptococci surface lipoteichoic acid by enzyme linked immune sorbent assay.

    abstract::An enzyme linked immune sorbent assay (ELISA) was developed to measure bacterial surface lipoteichoic acid (LTA). Numerous strains of oral streptococci belonging to the 'viridans' group were examined on three separate occasions. The results show that, under these cultural conditions, oral streptococci do not normally ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hogg SD,Manning JE

    更新日期:1989-04-01 00:00:00

  • vpsA- and luxO-independent biofilms of Vibrio cholerae.

    abstract::The natural life cycle of Vibrio cholerae involves the transitioning of cells between different environmental surfaces such as the chitinous shell of Crustaceae and the epithelial layer of the human intestine. Previous studies using static biofilm systems showed a strict dependence of biofilm formation on the vps and ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Müller J,Miller MC,Nielsen AT,Schoolnik GK,Spormann AM

    更新日期:2007-10-01 00:00:00

  • Properties of the periplasmic nitrate reductases from Paracoccus pantotrophus and Escherichia coli after growth in tungsten-supplemented media.

    abstract::Paracoccus pantotrophus grown anaerobically under denitrifying conditions expressed similar levels of the periplasmic nitrate reductase (NAP) when cultured in molybdate- or tungstate-containing media. A native PAGE gel stained for nitrate reductase activity revealed that only NapA from molybdate-grown cells displayed ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gates AJ,Hughes RO,Sharp SR,Millington PD,Nilavongse A,Cole JA,Leach ER,Jepson B,Richardson DJ,Butler CS

    更新日期:2003-03-28 00:00:00

  • Identification and characterization of a cross-reactive and a unique B-cell epitope on the hsp60 homologue from Neisseria gonorrhoeae.

    abstract::Two monoclonal antibodies (G6 and 7B), generated against a 63-kDa stress protein (GSP63) from Neisseria gonorrhoeae strain VP1, were used to investigate the antigenic heterogeneity of GSP63 among the Neisseriaceae and its antigenic relationship with the Hsp60 heat-shock protein family. Immunoblotting experiments demon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pannekoek Y,Dankert J,van Putten JP

    更新日期:1992-11-15 00:00:00

  • The alternative sigma factor RpoN regulates the quorum sensing gene rhlI in Pseudomonas aeruginosa.

    abstract::The rhl quorum sensing (QS) circuit of Pseudomonas aeruginosa is known to regulate the expression of a number of virulence factors. This study investigates the regulation of rhlI, encoding the auto-inducer synthase RhlI responsible for the synthesis of N-butryl-L-homoserine lactone (BHL). A putative RpoN binding site ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Thompson LS,Webb JS,Rice SA,Kjelleberg S

    更新日期:2003-03-28 00:00:00

  • A plasmid RK2-based broad-host-range cloning vector useful for transfer of metagenomic libraries to a variety of bacterial species.

    abstract::The majority of microorganisms in natural environments are difficult to cultivate, but their genes can be studied via metagenome libraries. To enhance the chances that these genes become expressed we here report the construction of a broad-host-range plasmid vector (pRS44) for fosmid and bacterial artificial chromosom...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Aakvik T,Degnes KF,Dahlsrud R,Schmidt F,Dam R,Yu L,Völker U,Ellingsen TE,Valla S

    更新日期:2009-06-01 00:00:00

  • In vitro activity of deferoxamine against Porphyromonas gingivalis.

    abstract::Deferoxamine (DFO), an FDA-approved iron chelator used for treatment of iron poisoning, affects bacteria as iron availability is intimately connected with growth and several virulence determinants. However, little is known about the effect on oral pathogens. In this study, the effect of DFO on Porphyromonas gingivalis...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Moon JH,Herr Y,Kim SW,Lee JY

    更新日期:2011-10-01 00:00:00

  • Attachment of bacteria to the roots of higher plants.

    abstract::Attachment of soil bacteria to plant cells is supposedly the very early step required in plant-microbe interactions. Attachment also is an initial step for the formation of microbial biofilms on plant roots. For the rhizobia-legume symbiosis, various mechanisms and diverse surface molecules of both partners have been ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Rodríguez-Navarro DN,Dardanelli MS,Ruíz-Saínz JE

    更新日期:2007-07-01 00:00:00

  • Characterization of a novel plasmid from extremely halophilic Archaea: nucleotide sequence and function analysis.

    abstract::We determined the complete nucleotide sequence of the 16341 bp plasmid pHH205 of the extremely halophilic archaeon Halobacterium salinarum J7. The plasmid has a G+C content of 61.1%. A number of direct and inverted repeat sequences were found in pHH205, while no insertion sequences were found. Thirty-eight large open ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ye X,Ou J,Ni L,Shi W,Shen P

    更新日期:2003-04-11 00:00:00

  • Successful enrichment of low-abundant comammox Nitrospira from nitrifying granules under ammonia-limited conditions.

    abstract::In artificial engineered systems, nitrification is a key reaction that accounts for the removal of biological nitrogen. Recently, a single microbe capable of oxidizing ammonia to nitrate, known as a complete ammonia oxidizer (comammox), has been discovered. Although the abundance and diversity of comammox Nitrospira i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fujitani H,Nomachi M,Takahashi Y,Hasebe Y,Eguchi M,Tsuneda S

    更新日期:2020-01-01 00:00:00

  • Characterization of Xanthomonas oryzae pv. oryzae recX, a gene that is required for high-level expression of recA.

    abstract::Analysis of the nucleotide sequence downstream from the Xanthomonas oryzae pv. oryzae recA gene reveals two orfs designated orfX and recX. The former has the potential to code for a 5.6 kDa protein of unknown function while the latter encodes for a putative 14.6 kDa protein with homology to RecX from various bacteria....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sukchawalit R,Vattanaviboon P,Utamapongchai S,Vaughn G,Mongkolsuk S

    更新日期:2001-11-27 00:00:00

  • Biological activity of the azlactone derivative EPA-35 against Trypanosoma cruzi.

    abstract::Chagas disease, caused by Trypanosoma cruzi, affects six to seven million people worldwide. Treatment is based on benznidazole, producing several side effects and debatable efficacy, highlighting the need for new alternative drugs. We investigated the activity of four C-4 functionalized azlactone derivatives (EPA-27, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: de Azeredo CM,Ávila EP,Pinheiro DL,Amarante GW,Soares MJ

    更新日期:2017-02-01 00:00:00

  • Enzymic activity of salivary amylase when bound to the surface of oral streptococci.

    abstract::The enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. Most strains able to bind amylase exhibited functional enzyme on their ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Douglas CW,Heath J,Gwynn JP

    更新日期:1992-04-15 00:00:00

  • Investigation of the role of ZurR in the physiology and pathogenesis of Listeria monocytogenes.

    abstract::Listeria monocytogenes is a Gram positive pathogen that is ubiquitous in the environment. It is a facultative anaerobic rod that causes listeriosis, a disease with potentially lethal consequences for susceptible individuals. During infection, the pathogen is capable of sequestering metal ions to act as vital biocataly...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dowd GC,Casey PG,Begley M,Hill C,Gahan CG

    更新日期:2012-02-01 00:00:00

  • Respective role of the 42- and 51-kDa components of the Bacillus sphaericus toxin overexpressed in Bacillus thuringiensis.

    abstract::The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nicolas L,Nielsen-Leroux C,Charles JF,Delécluse A

    更新日期:1993-02-01 00:00:00

  • Impala, a transposon from Fusarium oxysporum, is active in the genome of Penicillium griseoroseum.

    abstract::An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: de Queiroz MV,Daboussi MJ

    更新日期:2003-01-28 00:00:00

  • Borrelia burgdorferi possesses a collagenolytic activity.

    abstract::Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Grab DJ,Kennedy R,Philipp MT

    更新日期:1996-10-15 00:00:00

  • Discovery of novel Vibrio cholerae VSP-II genomic islands using comparative genomic analysis.

    abstract::This report describes Vibrio seventh pandemic island II (VSP-II) and three novel variants revealed by comparative genomics of 23 Vibrio cholerae strains and their presence among a large and diverse collection of V. cholerae isolates. Three VSP-II variants were reported previously and our results demonstrate the presen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Taviani E,Grim CJ,Choi J,Chun J,Haley B,Hasan NA,Huq A,Colwell RR

    更新日期:2010-07-01 00:00:00

  • Phylogenetic relationships between Tuber pseudoexcavatum, a Chinese truffle, and other Tuber species based on parsimony and distance analysis of four different gene sequences.

    abstract::Phylogenetic relationships between Tuber pseudoexcavatum and other Tuber species were investigated by studying the sequences of four genes: 5.8S-ITS2, beta-tubulin, protein kinase C and elongation factor 1alpha. The four phylogenetic trees allowed to differentiate the black truffle clade, composed of two subclades, on...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wang Y,Tan ZM,Zhang DC,Murat C,Jeandroz S,Le Tacon F

    更新日期:2006-06-01 00:00:00

  • Rapid distinction between Leptospira interrogans and Leptospira biflexa by PCR amplification of 23S ribosomal DNA.

    abstract::Bacterial specific primers were used to amplify 23S rRNA genes from a representative strain from each of the 23 serogroups of the pathogenic Leptospira interrogans and 8 strains from 6 serogroups of the non-pathogenic Leptospira biflexa. Only regions of extreme variability, which had been identified on the basis of ho...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Woo TH,Smythe LD,Symonds ML,Norris MA,Dohnt MF,Patel BK

    更新日期:1997-05-01 00:00:00

  • Growth, incidence and activities of dissimilatory sulfate-reducing bacteria in the human oral cavity.

    abstract::Viable counts and activities of sulfate-reducing bacteria were determined in the oral cavities of 12 healthy volunteers. Of these, 10 harboured viable sulfate-reducing bacteria populations. Six separate sites were sampled: the posterior tongue, anterior tongue, mid buccal mucosa, vestibular mucosa, supragingival plaqu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Willis CL,Gibson GR,Allison C,Macfarlane S,Holt JS

    更新日期:1995-06-15 00:00:00

  • The Salmonella typhimurium flgM gene, which encodes a negative regulator of flagella synthesis and is involved in virulence, is present and functional in other Salmonella species.

    abstract::FlgM inhibits the flagella-specific sigma factor FliA and is involved in the mouse-virulence of Salmonella typhimurium. In recent experiments, we observed that: (i) a flgM gene that could function to negatively regulate flagella synthesis was present in a variety of salmonellae; and (ii) the flgM gene derived from Sal...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schmitt CK,Darnell SC,O'Brien AD

    更新日期:1996-01-15 00:00:00

  • Initiation of sporulation in Clostridium difficile: a twist on the classic model.

    abstract::The formation of dormant endospores is a complex morphological process that permits long-term survival in inhospitable environments for many Gram-positive bacteria. Sporulation for the anaerobic gastrointestinal pathogen Clostridium difficile is necessary for survival outside of the gastrointestinal tract of its host....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Edwards AN,McBride SM

    更新日期:2014-09-01 00:00:00

  • Purification and partial characterization of an antigen specific to Lactobacillus brevis strains with beer spoilage activity.

    abstract::Certain Lactobacillus brevis strains are resistant to hop-derived compounds such as isohumulone and are able to grow in beer. In this study, we raised an antiserum against our beer spoilage laboratory strain L. brevis 578 which reacted with 23 of 24 beer spoilers and two of 13 non-spoilers in precipitation reactions u...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yasui T,Yoda K

    更新日期:1997-06-15 00:00:00